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The map label for this gene is pheS

Identifier: 194333009

GI number: 194333009

Start: 170642

End: 171673

Strand: Reverse

Name: pheS

Synonym: Paes_0163

Alternate gene names: 194333009

Gene position: 171673-170642 (Counterclockwise)

Preceding gene: 194333010

Following gene: 194332997

Centisome position: 6.83

GC content: 49.61

Gene sequence:

>1032_bases
ATGAAACACAGCATCGACTCTCTTCAGAAAGAAATCGAATCGTTTGCCATCACAAGCAACGAAACGCTCGAGGAGTTCAA
GCTCACCTACCTCGTCAGGAAAGGAAGCATCGCCAACCTCTTCAAACAGCTGAAGGACGTTTCGCCTGATGAACGCCGTG
CAGTAGGGCAGCTTCTGAACACCTTGAAAACCACCGCTGAAAAGAAGTATGAGGATGCCAAAGATACTCTTGCATCCTCA
GCTGCTTCAGAAAAAAACAACATCATAGATCTCACCCTGCCTGGAAGAACCCACTTTCTTGGTTCCGAACACCCGGTACA
GAAGGTCCTCGGCGATATGAAGCAGATCTTCACAGCCATGGGCTTTGCTATAGAAACAGGTCCGGAGCTTGAATGCGGCG
TTTACAACTTCGATAAGCTTAATTTCCCTCCTGACCATCCCGCAAGGGATATGCAGGACACCTTCTTCATCAAGCTCGAC
GAAAAAGATGGGAATGATGTTCTTCTCAGAACACACACCTCCCCGGTCCAGATTCGGGTCATGCTCGACCAGCGACCGCC
GATACGCGTTATCTGCCCTGGCAAAGTCTATCGCAACGAAGCGATCAGCTCCCGAAGCTATTGCGTCTTTCATCAACTCG
AAGGGCTCTATATTGATAAAAACGTCTCGTTTGCAGATCTGAAAGCAACCATCTACTCCTTTGCCAAACAGATGTTTGGC
CAGGACGTACAACTGCGATTCAGACCCAGCTTTTTCCCGTTCACCGAACCCTCCGCCGAGGTTGATGTCACCTGTTACCT
CTGCAACGGCAAGGGATGCAAAGTCTGCAAACAATCAGGATGGCTCGAAATTCTCGGGTGCGGCATGGTACACCCCAACG
TCCTCTGCCAATGCGGTATCGACCCTGAAGAATACTCAGGATATGCCTTCGGCATGGGTGTAGACAGAACGGCCCTGCTT
CGCTATAAGATAGACGATATACGCCTCCTGTTTGAAAACGATATACGAATGCTTGACCAGTTCATGCAGTAA

Upstream 100 bases:

>100_bases
ATCGCAAAGCGCTTGCAGAGATCGCAGTAAAAGACCCGTCTGCGTTCAGCCAGATCGTCAAAACAGCTATGGATTAAGCT
TTCAGAGTACCACTGTCCCT

Downstream 100 bases:

>100_bases
TAAAAAAAGCCTCATGTGATCATGAGGCTTTTTTTATTACTGCATATCTTGAACAGACAACAGGCAGCTACGTGTTCTTC
AATCCAATCGCCCAGGCTTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKHSIDSLQKEIESFAITSNETLEEFKLTYLVRKGSIANLFKQLKDVSPDERRAVGQLLNTLKTTAEKKYEDAKDTLASS
AASEKNNIIDLTLPGRTHFLGSEHPVQKVLGDMKQIFTAMGFAIETGPELECGVYNFDKLNFPPDHPARDMQDTFFIKLD
EKDGNDVLLRTHTSPVQIRVMLDQRPPIRVICPGKVYRNEAISSRSYCVFHQLEGLYIDKNVSFADLKATIYSFAKQMFG
QDVQLRFRPSFFPFTEPSAEVDVTCYLCNGKGCKVCKQSGWLEILGCGMVHPNVLCQCGIDPEEYSGYAFGMGVDRTALL
RYKIDDIRLLFENDIRMLDQFMQ

Sequences:

>Translated_343_residues
MKHSIDSLQKEIESFAITSNETLEEFKLTYLVRKGSIANLFKQLKDVSPDERRAVGQLLNTLKTTAEKKYEDAKDTLASS
AASEKNNIIDLTLPGRTHFLGSEHPVQKVLGDMKQIFTAMGFAIETGPELECGVYNFDKLNFPPDHPARDMQDTFFIKLD
EKDGNDVLLRTHTSPVQIRVMLDQRPPIRVICPGKVYRNEAISSRSYCVFHQLEGLYIDKNVSFADLKATIYSFAKQMFG
QDVQLRFRPSFFPFTEPSAEVDVTCYLCNGKGCKVCKQSGWLEILGCGMVHPNVLCQCGIDPEEYSGYAFGMGVDRTALL
RYKIDDIRLLFENDIRMLDQFMQ
>Mature_343_residues
MKHSIDSLQKEIESFAITSNETLEEFKLTYLVRKGSIANLFKQLKDVSPDERRAVGQLLNTLKTTAEKKYEDAKDTLASS
AASEKNNIIDLTLPGRTHFLGSEHPVQKVLGDMKQIFTAMGFAIETGPELECGVYNFDKLNFPPDHPARDMQDTFFIKLD
EKDGNDVLLRTHTSPVQIRVMLDQRPPIRVICPGKVYRNEAISSRSYCVFHQLEGLYIDKNVSFADLKATIYSFAKQMFG
QDVQLRFRPSFFPFTEPSAEVDVTCYLCNGKGCKVCKQSGWLEILGCGMVHPNVLCQCGIDPEEYSGYAFGMGVDRTALL
RYKIDDIRLLFENDIRMLDQFMQ

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=30.2158273381295, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI5729820, Length=241, Percent_Identity=29.8755186721992, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788007, Length=322, Percent_Identity=45.6521739130435, Blast_Score=296, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI32563657, Length=277, Percent_Identity=30.6859205776173, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=277, Percent_Identity=30.6859205776173, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=279, Percent_Identity=30.4659498207885, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=275, Percent_Identity=30.5454545454545, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=38.6138613861386, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858079, Length=291, Percent_Identity=30.2405498281787, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=27.9527559055118, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PROA2 (B4S3C8)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002014869.1
- ProteinModelPortal:   B4S3C8
- SMR:   B4S3C8
- GeneID:   6459084
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0163
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38949; Mature: 38949

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSIDSLQKEIESFAITSNETLEEFKLTYLVRKGSIANLFKQLKDVSPDERRAVGQLLN
CCCCHHHHHHHHHHHCCCCCCHHHHHEEEHEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
TLKTTAEKKYEDAKDTLASSAASEKNNIIDLTLPGRTHFLGSEHPVQKVLGDMKQIFTAM
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
GFAIETGPELECGVYNFDKLNFPPDHPARDMQDTFFIKLDEKDGNDVLLRTHTSPVQIRV
CEEECCCCCCEECEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEE
MLDQRPPIRVICPGKVYRNEAISSRSYCVFHQLEGLYIDKNVSFADLKATIYSFAKQMFG
EECCCCCEEEECCCHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCC
QDVQLRFRPSFFPFTEPSAEVDVTCYLCNGKGCKVCKQSGWLEILGCGMVHPNVLCQCGI
CCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCEEEEECCCCCCCCEEECCC
DPEEYSGYAFGMGVDRTALLRYKIDDIRLLFENDIRMLDQFMQ
CHHHCCCEEEECCCCCEEEEEEEHHHEEEEHHHHHHHHHHHHC
>Mature Secondary Structure
MKHSIDSLQKEIESFAITSNETLEEFKLTYLVRKGSIANLFKQLKDVSPDERRAVGQLLN
CCCCHHHHHHHHHHHCCCCCCHHHHHEEEHEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
TLKTTAEKKYEDAKDTLASSAASEKNNIIDLTLPGRTHFLGSEHPVQKVLGDMKQIFTAM
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
GFAIETGPELECGVYNFDKLNFPPDHPARDMQDTFFIKLDEKDGNDVLLRTHTSPVQIRV
CEEECCCCCCEECEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEE
MLDQRPPIRVICPGKVYRNEAISSRSYCVFHQLEGLYIDKNVSFADLKATIYSFAKQMFG
EECCCCCEEEECCCHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCC
QDVQLRFRPSFFPFTEPSAEVDVTCYLCNGKGCKVCKQSGWLEILGCGMVHPNVLCQCGI
CCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCEEEEECCCCCCCCEEECCC
DPEEYSGYAFGMGVDRTALLRYKIDDIRLLFENDIRMLDQFMQ
CHHHCCCEEEECCCCCEEEEEEEHHHEEEEHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA