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The map label for this gene is ykfA [H]

Identifier: 194332901

GI number: 194332901

Start: 51861

End: 52805

Strand: Reverse

Name: ykfA [H]

Synonym: Paes_0048

Alternate gene names: 194332901

Gene position: 52805-51861 (Counterclockwise)

Preceding gene: 194332902

Following gene: 194332900

Centisome position: 2.1

GC content: 52.38

Gene sequence:

>945_bases
ATGAAAACACTTGTCCCAAAAGCCCTTCGCCCCGGAGATATCATAGGCCTCGTTTCGCCATCATCCCCATCTCCTGACAA
GGAACGGATCCAGCAGGCTGTCCTCTATCTTGAAAAAATCGGCTACCGCGTCAAACCGGCAAGACACTTCAACTGCAGAG
CCGACAACCGTCATGAACTTGACCGCCAGAAACTCCATGACCTGCATGAAATGTTTGCAGACCGGTCAGTCAAAGGGATT
TTCTGCCTGAGAGGCGGATCGGGCGCAACGCGCCTGCTTCAGGATATCGATTACGATCTGATATCGAAAAATCCGAAAAT
TCTGGCTGGATATTCCGATATTACAGCCCTCTCCCTGGCGATCTATGCCCGATCGGGCCTGATCACCTTTTCCGGCCCCA
TGCTGGCAACCGAACTGCACGCACCAAGCCCCTATACCGAGGAGCACTTCTGGACAATGATTTCCGATCCGGACCGCACC
CATGAAATTGTCAACCATTCTGAACACCCGAGAAACATTCTCCGCCCAGGCTCCGGCGAAGGAATCCTGATTGCAGGAAA
TCTCAGCGTTCTCTCCTGTCTGGCAGGAACCCCGTTCATGCCATCGACAAAGGAAGCAATTCTGCTCTTCGAGGATATCA
ACGAAGAACCTTATCGTATCGACCGCCTGTTATCCCACCTCTACAATGCAGGACTGATTGGCCACTGCCAGGGACTGCTG
TTCGGCCAGTTCCGGAGAGAAGCCATGCGCTTCGACTGCCAGCAGCCGTTAATCGATATTTTTCATTATTACACCGCAAA
AATCCAGAACAGCATCCCCGTCGCCGCAGGCATTTCTTACGGACATATCGAGAACATGTTCACCCTCCCCATCGGGGCAA
GTTGCCGTATTGTGGCCTCAGATGAAAATCTTGTCATCACAACGACAGCTGCTGTAACCCGCTGA

Upstream 100 bases:

>100_bases
CAAACATCAGTGAATCGGGATTATACCGATTCACTGATGTTTTTTTATTTTGAACGGTACCGTCCTCTTCAATCAAGTAG
AACGGATCAGTCCGTATCGT

Downstream 100 bases:

>100_bases
ATTCAGGAACAGCATTTTCCCGTCAACAATGTTTTTATTACTATAGATTGAGTAGTCTGCTCAGAACAAACAGCTCGGTA
TATGGAAAACATGTGGCAGT

Product: Muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKTLVPKALRPGDIIGLVSPSSPSPDKERIQQAVLYLEKIGYRVKPARHFNCRADNRHELDRQKLHDLHEMFADRSVKGI
FCLRGGSGATRLLQDIDYDLISKNPKILAGYSDITALSLAIYARSGLITFSGPMLATELHAPSPYTEEHFWTMISDPDRT
HEIVNHSEHPRNILRPGSGEGILIAGNLSVLSCLAGTPFMPSTKEAILLFEDINEEPYRIDRLLSHLYNAGLIGHCQGLL
FGQFRREAMRFDCQQPLIDIFHYYTAKIQNSIPVAAGISYGHIENMFTLPIGASCRIVASDENLVITTTAAVTR

Sequences:

>Translated_314_residues
MKTLVPKALRPGDIIGLVSPSSPSPDKERIQQAVLYLEKIGYRVKPARHFNCRADNRHELDRQKLHDLHEMFADRSVKGI
FCLRGGSGATRLLQDIDYDLISKNPKILAGYSDITALSLAIYARSGLITFSGPMLATELHAPSPYTEEHFWTMISDPDRT
HEIVNHSEHPRNILRPGSGEGILIAGNLSVLSCLAGTPFMPSTKEAILLFEDINEEPYRIDRLLSHLYNAGLIGHCQGLL
FGQFRREAMRFDCQQPLIDIFHYYTAKIQNSIPVAAGISYGHIENMFTLPIGASCRIVASDENLVITTTAAVTR
>Mature_314_residues
MKTLVPKALRPGDIIGLVSPSSPSPDKERIQQAVLYLEKIGYRVKPARHFNCRADNRHELDRQKLHDLHEMFADRSVKGI
FCLRGGSGATRLLQDIDYDLISKNPKILAGYSDITALSLAIYARSGLITFSGPMLATELHAPSPYTEEHFWTMISDPDRT
HEIVNHSEHPRNILRPGSGEGILIAGNLSVLSCLAGTPFMPSTKEAILLFEDINEEPYRIDRLLSHLYNAGLIGHCQGLL
FGQFRREAMRFDCQQPLIDIFHYYTAKIQNSIPVAAGISYGHIENMFTLPIGASCRIVASDENLVITTTAAVTR

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=302, Percent_Identity=30.4635761589404, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34982; Mature: 34982

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLVPKALRPGDIIGLVSPSSPSPDKERIQQAVLYLEKIGYRVKPARHFNCRADNRHEL
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
DRQKLHDLHEMFADRSVKGIFCLRGGSGATRLLQDIDYDLISKNPKILAGYSDITALSLA
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCEEECCCHHHHHHHH
IYARSGLITFSGPMLATELHAPSPYTEEHFWTMISDPDRTHEIVNHSEHPRNILRPGSGE
HHHHCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHCCCCCCHHHCCCCCCC
GILIAGNLSVLSCLAGTPFMPSTKEAILLFEDINEEPYRIDRLLSHLYNAGLIGHCQGLL
EEEEECCHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHH
FGQFRREAMRFDCQQPLIDIFHYYTAKIQNSIPVAAGISYGHIENMFTLPIGASCRIVAS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHCCEEEECCCCCEEEEEC
DENLVITTTAAVTR
CCCEEEEECCCCCC
>Mature Secondary Structure
MKTLVPKALRPGDIIGLVSPSSPSPDKERIQQAVLYLEKIGYRVKPARHFNCRADNRHEL
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
DRQKLHDLHEMFADRSVKGIFCLRGGSGATRLLQDIDYDLISKNPKILAGYSDITALSLA
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCEEECCCHHHHHHHH
IYARSGLITFSGPMLATELHAPSPYTEEHFWTMISDPDRTHEIVNHSEHPRNILRPGSGE
HHHHCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHCCCCCCHHHCCCCCCC
GILIAGNLSVLSCLAGTPFMPSTKEAILLFEDINEEPYRIDRLLSHLYNAGLIGHCQGLL
EEEEECCHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHH
FGQFRREAMRFDCQQPLIDIFHYYTAKIQNSIPVAAGISYGHIENMFTLPIGASCRIVAS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHCCEEEECCCCCEEEEEC
DENLVITTTAAVTR
CCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]