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The map label for this gene is ptsI [H]

Identifier: 193213469

GI number: 193213469

Start: 2010621

End: 2012420

Strand: Direct

Name: ptsI [H]

Synonym: Cpar_1830

Alternate gene names: 193213469

Gene position: 2010621-2012420 (Clockwise)

Preceding gene: 193213468

Following gene: 193213472

Centisome position: 87.83

GC content: 58.5

Gene sequence:

>1800_bases
ATGGTTTACAAAAAAGCACCGAACCCCTCCGGCGATGTGCCGGACGCCAGTGAAACCCCCGGCCATCAATTCGGCCCGGC
AGCAAAGGAACGCCGCTACACCGGCATCGGCAGTTCGAAAGGTTTCGCGATCGGCGAGGCCCACGAGTTCGTTCAGGAGA
TCATCGATCATGAAATCGCTGAACTGAACGAGAGCAACATCGAGGAAGAGATCGAGCGCTTTCAGGCCGCCCTGAACCGC
TCGGAAAAGGAGCTGAAAAAGATCGAGAGGGTCACCACCCGCAAAATCGGCAAGCTCTATTCCGACCTGTTCCAGGCGCA
GATCATGCTGCTCAACGATCCGGTGCTGATCGAAAACATCACCCGCCGCATCCGCGAAGAGCTGAAACCGGCACATCTGG
TCATCGAGCAGGAGTTCGAGCAGTACCTCGGCAACTTCATCCACTCCGACGACCAGATTTTCCGCGAACGGGCCGCCGAC
CTGCACGACATCAAGGAGCGGATCATCCGGAACCTGCACATCCGGAAACTGCACTCCTGGGTGCCCGAAGGCACCATCGT
GGTCTCACACCACCTCTCGCCAGCAGACATCATTCTGCTCAGCCGCAGCAACGTCAAAGGGTTCGCCACCGACACCGGCG
GCAAAACTTCGCACGTCGCCCTGATCTGCAAGTCGCTCAACATTCCGATGGTGGCCGGTCTCGGCAACTTCTCGCAGAAG
ATCGGTTCGGGAATGCCCGTCATTCTTGACGGCACGGAGGGGGTGATCATCACCGAGCCAAGTGAGCAGACCATCGGCGA
GTACCAGCAAAAGCGGGAGGACGTAATCCGTCGGGAAGCCGACGATTCGGCGCTGGCGCATCAAAACGCATTCACGCGCT
GCGGCATGCGGATCACGGTCTGCTCAAACATCGACTTCAAGGAGGAGATCGAACACCTAGAACCAACCGGTTCGGAAGGT
GTCGGACTGTTCCGGACGGAGAACCTCTTCCTCGACGACCTCAAACCGCCGCAGGAGTCGGTGCAGCAGGAGTACTACCT
CGAAATGGCCGAGATGCTCAGCCCGAAACCGCTGGTCATTCGGCTGTTTGACATCGGCGGCGACAAACTGATCTACTCGC
CGGTCAAGGAGCCGAACCCGAACCTCGGCTGGCGAGGCGTCCGGATTCTGATCGACGTGCCGGAAATTCTCGACGCGCAG
CTCCGCTCCATCATCAAAGCCAACGTTCACGGCAACGTCGATCTGATGATTCCCATGATCTCGTCGCTTGAAGAGATCGA
ACACATCAAAGAGCGCCTCGAACACCACTACAAGCGCATCAGCGAAGCGTCGCACGAAACGATCTACAAGCCCGGCCTCG
GAGCGATGATCGAAATGCCGGCGGCCGTCGAACTGATCGATGAAATCACCAGCATGGTCGATTTCATCAGTATCGGAACC
AACGATCTGACCCAGTACACGCTGGCCGTTGACCGCAATAACCTGATCGTGCAGGATCTGTTCGAGAAGTTCCACCCGGC
CATCATCCGCCAGCTCTACCGTATCATCTCGACGGCGCAGAAAAACCGCTGCCGGGCAACCCTCTGCGGCGATATGGGCT
CCGATCCGCTGGCCACACCGTTCCTGATCGGATGCGGTCTCCGGGAGTTCAGCATCGTCAGCTCCGACATCCCGGCGCTC
AAGGCGCGGGTAGCCAATCATTCAATCACTGAGTGTGAAGCGCTGGCCGCCGAGTGCATCAAACTCTCCAGCCCTCAGGC
CATCAAAGCCCGCCTCGAAGTGTTCCTCAAGGAGCACTGA

Upstream 100 bases:

>100_bases
CAGCGAGCTTTCCGGAGCCGCCATCCCTGCCCGAGCCGCCGCCGATTGACGACAGCTATCTCAACAACACGGATACCGCA
CCGTTTTAGCTCTTCCGTCT

Downstream 100 bases:

>100_bases
GGCGGATCGGAAGGCGCGAGCAGGCTGTCGGACTTATCGGGAAAAAACATCTCATCCGGAGTATGATCCAGCTTTTGGTC
ATTCCCGCGTAGGCGGAAAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MVYKKAPNPSGDVPDASETPGHQFGPAAKERRYTGIGSSKGFAIGEAHEFVQEIIDHEIAELNESNIEEEIERFQAALNR
SEKELKKIERVTTRKIGKLYSDLFQAQIMLLNDPVLIENITRRIREELKPAHLVIEQEFEQYLGNFIHSDDQIFRERAAD
LHDIKERIIRNLHIRKLHSWVPEGTIVVSHHLSPADIILLSRSNVKGFATDTGGKTSHVALICKSLNIPMVAGLGNFSQK
IGSGMPVILDGTEGVIITEPSEQTIGEYQQKREDVIRREADDSALAHQNAFTRCGMRITVCSNIDFKEEIEHLEPTGSEG
VGLFRTENLFLDDLKPPQESVQQEYYLEMAEMLSPKPLVIRLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQ
LRSIIKANVHGNVDLMIPMISSLEEIEHIKERLEHHYKRISEASHETIYKPGLGAMIEMPAAVELIDEITSMVDFISIGT
NDLTQYTLAVDRNNLIVQDLFEKFHPAIIRQLYRIISTAQKNRCRATLCGDMGSDPLATPFLIGCGLREFSIVSSDIPAL
KARVANHSITECEALAAECIKLSSPQAIKARLEVFLKEH

Sequences:

>Translated_599_residues
MVYKKAPNPSGDVPDASETPGHQFGPAAKERRYTGIGSSKGFAIGEAHEFVQEIIDHEIAELNESNIEEEIERFQAALNR
SEKELKKIERVTTRKIGKLYSDLFQAQIMLLNDPVLIENITRRIREELKPAHLVIEQEFEQYLGNFIHSDDQIFRERAAD
LHDIKERIIRNLHIRKLHSWVPEGTIVVSHHLSPADIILLSRSNVKGFATDTGGKTSHVALICKSLNIPMVAGLGNFSQK
IGSGMPVILDGTEGVIITEPSEQTIGEYQQKREDVIRREADDSALAHQNAFTRCGMRITVCSNIDFKEEIEHLEPTGSEG
VGLFRTENLFLDDLKPPQESVQQEYYLEMAEMLSPKPLVIRLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQ
LRSIIKANVHGNVDLMIPMISSLEEIEHIKERLEHHYKRISEASHETIYKPGLGAMIEMPAAVELIDEITSMVDFISIGT
NDLTQYTLAVDRNNLIVQDLFEKFHPAIIRQLYRIISTAQKNRCRATLCGDMGSDPLATPFLIGCGLREFSIVSSDIPAL
KARVANHSITECEALAAECIKLSSPQAIKARLEVFLKEH
>Mature_599_residues
MVYKKAPNPSGDVPDASETPGHQFGPAAKERRYTGIGSSKGFAIGEAHEFVQEIIDHEIAELNESNIEEEIERFQAALNR
SEKELKKIERVTTRKIGKLYSDLFQAQIMLLNDPVLIENITRRIREELKPAHLVIEQEFEQYLGNFIHSDDQIFRERAAD
LHDIKERIIRNLHIRKLHSWVPEGTIVVSHHLSPADIILLSRSNVKGFATDTGGKTSHVALICKSLNIPMVAGLGNFSQK
IGSGMPVILDGTEGVIITEPSEQTIGEYQQKREDVIRREADDSALAHQNAFTRCGMRITVCSNIDFKEEIEHLEPTGSEG
VGLFRTENLFLDDLKPPQESVQQEYYLEMAEMLSPKPLVIRLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQ
LRSIIKANVHGNVDLMIPMISSLEEIEHIKERLEHHYKRISEASHETIYKPGLGAMIEMPAAVELIDEITSMVDFISIGT
NDLTQYTLAVDRNNLIVQDLFEKFHPAIIRQLYRIISTAQKNRCRATLCGDMGSDPLATPFLIGCGLREFSIVSSDIPAL
KARVANHSITECEALAAECIKLSSPQAIKARLEVFLKEH

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=569, Percent_Identity=31.9859402460457, Blast_Score=305, Evalue=8e-84,
Organism=Escherichia coli, GI1789193, Length=586, Percent_Identity=30.2047781569966, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI1788726, Length=511, Percent_Identity=33.8551859099804, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI48994992, Length=501, Percent_Identity=32.3353293413174, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1787994, Length=444, Percent_Identity=23.4234234234234, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 67295; Mature: 67295

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYKKAPNPSGDVPDASETPGHQFGPAAKERRYTGIGSSKGFAIGEAHEFVQEIIDHEIA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ELNESNIEEEIERFQAALNRSEKELKKIERVTTRKIGKLYSDLFQAQIMLLNDPVLIENI
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TRRIREELKPAHLVIEQEFEQYLGNFIHSDDQIFRERAADLHDIKERIIRNLHIRKLHSW
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPEGTIVVSHHLSPADIILLSRSNVKGFATDTGGKTSHVALICKSLNIPMVAGLGNFSQK
CCCCEEEEEECCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHH
IGSGMPVILDGTEGVIITEPSEQTIGEYQQKREDVIRREADDSALAHQNAFTRCGMRITV
HCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEE
CSNIDFKEEIEHLEPTGSEGVGLFRTENLFLDDLKPPQESVQQEYYLEMAEMLSPKPLVI
ECCCCHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEE
RLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQLRSIIKANVHGNVDLMIPMI
EEEECCCCEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEHHHHH
SSLEEIEHIKERLEHHYKRISEASHETIYKPGLGAMIEMPAAVELIDEITSMVDFISIGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCC
NDLTQYTLAVDRNNLIVQDLFEKFHPAIIRQLYRIISTAQKNRCRATLCGDMGSDPLATP
CCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCH
FLIGCGLREFSIVSSDIPALKARVANHSITECEALAAECIKLSSPQAIKARLEVFLKEH
HHHHCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVYKKAPNPSGDVPDASETPGHQFGPAAKERRYTGIGSSKGFAIGEAHEFVQEIIDHEIA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ELNESNIEEEIERFQAALNRSEKELKKIERVTTRKIGKLYSDLFQAQIMLLNDPVLIENI
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TRRIREELKPAHLVIEQEFEQYLGNFIHSDDQIFRERAADLHDIKERIIRNLHIRKLHSW
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPEGTIVVSHHLSPADIILLSRSNVKGFATDTGGKTSHVALICKSLNIPMVAGLGNFSQK
CCCCEEEEEECCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHH
IGSGMPVILDGTEGVIITEPSEQTIGEYQQKREDVIRREADDSALAHQNAFTRCGMRITV
HCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEE
CSNIDFKEEIEHLEPTGSEGVGLFRTENLFLDDLKPPQESVQQEYYLEMAEMLSPKPLVI
ECCCCHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEE
RLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQLRSIIKANVHGNVDLMIPMI
EEEECCCCEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEHHHHH
SSLEEIEHIKERLEHHYKRISEASHETIYKPGLGAMIEMPAAVELIDEITSMVDFISIGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCC
NDLTQYTLAVDRNNLIVQDLFEKFHPAIIRQLYRIISTAQKNRCRATLCGDMGSDPLATP
CCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCH
FLIGCGLREFSIVSSDIPALKARVANHSITECEALAAECIKLSSPQAIKARLEVFLKEH
HHHHCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]