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The map label for this gene is epsE [H]

Identifier: 193213453

GI number: 193213453

Start: 1987613

End: 1989130

Strand: Direct

Name: epsE [H]

Synonym: Cpar_1814

Alternate gene names: 193213453

Gene position: 1987613-1989130 (Clockwise)

Preceding gene: 193213451

Following gene: 193213455

Centisome position: 86.82

GC content: 47.3

Gene sequence:

>1518_bases
ATGAACCGTCTTTCCGGAATCATCAGACAGCAGGCAAAACATGCCGAGCGAAAAGTTTCGCGCGTTAAATGGCAGCTTCG
TTATGCCTTGGTAAAGAAGGCTCTGAAGAAAAGACCTGAGCATCCCGCGCTTCTGAACAAGCTTGCAAAGATACTTGTAA
AGCTGAAAGATGAACAAGAGTTGCTTCGAGCGACAGAACGTTATGCCATGAATGGATTCAGGCATGACGACCCGCAAACT
GCCCAAGCGCTCTTTCGCCTTGCGCATTCAAGAGCGGCTTCATTCTGGGATAATGGCAGTTATGAGGAGGCTGTAGCGCT
GATTAACAGCGTCATGGAGTGGAATACACCTAATAGCAGTATGCTGCATCAATCGCTTTACTGGCAGTTATGTATAGCAA
AAACAGAAAACAAGAAGAATGTCATCGAGAAACGAGTACTTGACCGGTATGTTGAAATCGAAAAGCAGCATTTACCGCAC
GACTTTGTAAAAGCTCACATGGCAACCGAGGCGATGATGCGTCTCGGCCTTGCTGATCAGGCTAAAAAGACCCTCCTGAA
GCACGAAGACAAGCCAATGGCGCTTTTGGCCCTGTCCAATACCAGCATTGATAATGACGAGCTGTGGCTCGAGTATGTCA
ACCGTTTCTTCAGTTCTCGAAAGCTGGAACCGCTCTCCCTATTTGAGGGTGTCAAGCCACGCTTTCTCCGCCTTGAAGGA
GCCCCGGCTACCTCATACAGCGGTGGCCCGAAAATCAGCGTCATCATGACCGCATTCAACGTCGAAAACTATATTGAAAC
AGCAATACGCTCTGTACTGGCTCAATCATGGAGTAATTTCGAACTGATCGTGGTTGATGACTGCAGCACAGACGCTACTC
GCCGTATCGTAAAACAATTGATGGAACATGACTCAAGAATCAAGCTTATAGAAAACCAAACAAATTGCGGCACCTATATC
AACCGGAACAAGGCTTATGACCTGGCGACAGGCGAATATGTAACCTGCCATGATTCTGATGACTGGGCACACCCGAAAAA
GCTCGAATATCAAGTTACGGCGCTACTTAAAAATCCTGACGCTGTTTCAAGCTCGAGCCACTGGGTGCGAATGTATGAAA
ACGGCCAGTTCATTTTTTATACCCTTGGCACTTTTGCCCAATACAATGCGAACTCGCTGATGTTCAAAATCGACAGGGTA
AAACCGGTGCTTGGTTACTGGGACTCAGTACGAATAAGCGCTGACAGCGAATTCATCAGGAGGCTGCACGCCGTATTTGG
CAAAGAGCGTGAAATCATCCTCGATGACGTTCTTATGTTTGGTCTTAAACGTCCGGAAAGCCTTACAACAGCATCAGGAA
GCGGTCACAGGCCTAACGGCATCTCTCCCTTGCGCAAAAGATATTATGACAGTTATACCAACTGGCATCAAACCATTGAC
AAGAATTCGGCATACATGGCATTTCCTCTGAAAAGTCGGCCATTCGAAGCACCTGAAGAGATACTGGCTAAAGGCTGA

Upstream 100 bases:

>100_bases
AAAAGCACTGTACTGGCGAACAAATATTGAACTCTCTTACAAAAAAGACCATATTTATAAAGTAAAGCCCCCCTACCCCG
CGAACATATTGAAAAGTTCC

Downstream 100 bases:

>100_bases
AGGTTCCTACCCGAAAAAACATCTCATTCCAGAAACCCGTCAGCGGAAATTGTGAACACGCCCGCCTTGTTAGCCGAGAT
TGCTCTGACCTCCCAAGCGT

Product: family 2 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNRLSGIIRQQAKHAERKVSRVKWQLRYALVKKALKKRPEHPALLNKLAKILVKLKDEQELLRATERYAMNGFRHDDPQT
AQALFRLAHSRAASFWDNGSYEEAVALINSVMEWNTPNSSMLHQSLYWQLCIAKTENKKNVIEKRVLDRYVEIEKQHLPH
DFVKAHMATEAMMRLGLADQAKKTLLKHEDKPMALLALSNTSIDNDELWLEYVNRFFSSRKLEPLSLFEGVKPRFLRLEG
APATSYSGGPKISVIMTAFNVENYIETAIRSVLAQSWSNFELIVVDDCSTDATRRIVKQLMEHDSRIKLIENQTNCGTYI
NRNKAYDLATGEYVTCHDSDDWAHPKKLEYQVTALLKNPDAVSSSSHWVRMYENGQFIFYTLGTFAQYNANSLMFKIDRV
KPVLGYWDSVRISADSEFIRRLHAVFGKEREIILDDVLMFGLKRPESLTTASGSGHRPNGISPLRKRYYDSYTNWHQTID
KNSAYMAFPLKSRPFEAPEEILAKG

Sequences:

>Translated_505_residues
MNRLSGIIRQQAKHAERKVSRVKWQLRYALVKKALKKRPEHPALLNKLAKILVKLKDEQELLRATERYAMNGFRHDDPQT
AQALFRLAHSRAASFWDNGSYEEAVALINSVMEWNTPNSSMLHQSLYWQLCIAKTENKKNVIEKRVLDRYVEIEKQHLPH
DFVKAHMATEAMMRLGLADQAKKTLLKHEDKPMALLALSNTSIDNDELWLEYVNRFFSSRKLEPLSLFEGVKPRFLRLEG
APATSYSGGPKISVIMTAFNVENYIETAIRSVLAQSWSNFELIVVDDCSTDATRRIVKQLMEHDSRIKLIENQTNCGTYI
NRNKAYDLATGEYVTCHDSDDWAHPKKLEYQVTALLKNPDAVSSSSHWVRMYENGQFIFYTLGTFAQYNANSLMFKIDRV
KPVLGYWDSVRISADSEFIRRLHAVFGKEREIILDDVLMFGLKRPESLTTASGSGHRPNGISPLRKRYYDSYTNWHQTID
KNSAYMAFPLKSRPFEAPEEILAKG
>Mature_505_residues
MNRLSGIIRQQAKHAERKVSRVKWQLRYALVKKALKKRPEHPALLNKLAKILVKLKDEQELLRATERYAMNGFRHDDPQT
AQALFRLAHSRAASFWDNGSYEEAVALINSVMEWNTPNSSMLHQSLYWQLCIAKTENKKNVIEKRVLDRYVEIEKQHLPH
DFVKAHMATEAMMRLGLADQAKKTLLKHEDKPMALLALSNTSIDNDELWLEYVNRFFSSRKLEPLSLFEGVKPRFLRLEG
APATSYSGGPKISVIMTAFNVENYIETAIRSVLAQSWSNFELIVVDDCSTDATRRIVKQLMEHDSRIKLIENQTNCGTYI
NRNKAYDLATGEYVTCHDSDDWAHPKKLEYQVTALLKNPDAVSSSSHWVRMYENGQFIFYTLGTFAQYNANSLMFKIDRV
KPVLGYWDSVRISADSEFIRRLHAVFGKEREIILDDVLMFGLKRPESLTTASGSGHRPNGISPLRKRYYDSYTNWHQTID
KNSAYMAFPLKSRPFEAPEEILAKG

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788372, Length=101, Percent_Identity=43.5643564356436, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790044, Length=120, Percent_Identity=32.5, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787259, Length=121, Percent_Identity=34.7107438016529, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 58215; Mature: 58215

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLSGIIRQQAKHAERKVSRVKWQLRYALVKKALKKRPEHPALLNKLAKILVKLKDEQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHH
LLRATERYAMNGFRHDDPQTAQALFRLAHSRAASFWDNGSYEEAVALINSVMEWNTPNSS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHH
MLHQSLYWQLCIAKTENKKNVIEKRVLDRYVEIEKQHLPHDFVKAHMATEAMMRLGLADQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHH
AKKTLLKHEDKPMALLALSNTSIDNDELWLEYVNRFFSSRKLEPLSLFEGVKPRFLRLEG
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEC
APATSYSGGPKISVIMTAFNVENYIETAIRSVLAQSWSNFELIVVDDCSTDATRRIVKQL
CCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
MEHDSRIKLIENQTNCGTYINRNKAYDLATGEYVTCHDSDDWAHPKKLEYQVTALLKNPD
HCCCCCEEEEECCCCCCCEECCCCCEEECCCCEEEEECCCCCCCCCHHHEEEEEEECCCC
AVSSSSHWVRMYENGQFIFYTLGTFAQYNANSLMFKIDRVKPVLGYWDSVRISADSEFIR
CCCCCCCEEEEEECCCEEEEEECCHHHCCCCEEEEEEECCCHHHCCCCCEEECCCHHHHH
RLHAVFGKEREIILDDVLMFGLKRPESLTTASGSGHRPNGISPLRKRYYDSYTNWHQTID
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
KNSAYMAFPLKSRPFEAPEEILAKG
CCCCEEEEECCCCCCCCCHHHHCCC
>Mature Secondary Structure
MNRLSGIIRQQAKHAERKVSRVKWQLRYALVKKALKKRPEHPALLNKLAKILVKLKDEQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHH
LLRATERYAMNGFRHDDPQTAQALFRLAHSRAASFWDNGSYEEAVALINSVMEWNTPNSS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHH
MLHQSLYWQLCIAKTENKKNVIEKRVLDRYVEIEKQHLPHDFVKAHMATEAMMRLGLADQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHH
AKKTLLKHEDKPMALLALSNTSIDNDELWLEYVNRFFSSRKLEPLSLFEGVKPRFLRLEG
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEC
APATSYSGGPKISVIMTAFNVENYIETAIRSVLAQSWSNFELIVVDDCSTDATRRIVKQL
CCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
MEHDSRIKLIENQTNCGTYINRNKAYDLATGEYVTCHDSDDWAHPKKLEYQVTALLKNPD
HCCCCCEEEEECCCCCCCEECCCCCEEECCCCEEEEECCCCCCCCCHHHEEEEEEECCCC
AVSSSSHWVRMYENGQFIFYTLGTFAQYNANSLMFKIDRVKPVLGYWDSVRISADSEFIR
CCCCCCCEEEEEECCCEEEEEECCHHHCCCCEEEEEEECCCHHHCCCCCEEECCCHHHHH
RLHAVFGKEREIILDDVLMFGLKRPESLTTASGSGHRPNGISPLRKRYYDSYTNWHQTID
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
KNSAYMAFPLKSRPFEAPEEILAKG
CCCCEEEEECCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]