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The map label for this gene is miaA

Identifier: 193212890

GI number: 193212890

Start: 1354082

End: 1355023

Strand: Direct

Name: miaA

Synonym: Cpar_1238

Alternate gene names: 193212890

Gene position: 1354082-1355023 (Clockwise)

Preceding gene: 193212889

Following gene: 193212891

Centisome position: 59.15

GC content: 58.6

Gene sequence:

>942_bases
ATGAGCCAGAAACCTGTCCTCGTCATCCTCGGCCCCACCGCCTCCGGCAAAACGGAGCTGGCCTTCCGCATCGCCCGTAA
AACCGGCGGAGAAATCATCTCGGCCGACTCGCGCCAGATCTATCGCAGCATGGACATCGGCACCGCCAAGCCGCCGAAGT
GGATGCTCAATGAAGTCAAACACCATTTCATCGACGAAAAGGAGATCGGCGAACCCTTCAGCGCGGGAGATTTCGCAGAA
CAGGCGGCAGAGAGAATCCGCGAACTACGTCAGCGCGGCATTACACCTATCGTGGCGGGCGGCTCGACGCTCTACCTCGA
AGGCCTGCTCAAAGGCTTCGCCGAACTGCCGCAATCCAATCCGGAGATCAGAGCGCGGCTGAAGCATGAACTTGAGCTGC
ACGGCGCAGAAGCGCTCTACCGACGGCTCGAAGCGTTCGACCCGGAGCAGGCCAAAACCCTCGACCCCACCAAAACCCAG
CGCCTCATCCGGAGCCTCGAAATCATCGAAATTTCGGGAACGACGGTCACCGCCCTGCAACGCAAAACCTCCGGCCCGCC
GCCCGGCATCGAGTTCACCGTCATCGCCCTCGATCTGCCCCGCGAAGTGCTCTACGAGCGCATCAACCGACGCACAAGCG
AAATGATGCACGCCGGACTCGAAGCCGAAACCCGCCATCTTTTCGACAAGTTTCGTGACGAATGGCGCAGCAAAAAACTC
AACGCCCTTGCTACCGTTGGCTATCGCGAACTCTTCGAACACTTCGAAGGGCTGTACAATTTGGAGACCGCTGAAGCCCT
TATCGCCCAGCACACCCGCAACTATGCCAAACGCCAGTTGACATTTTTCCGTAACCGACTCGATGTGGAATGGGTAAAAG
GCCCGCTTGATGAAGCCGGGATTGAGGCGCTGGTTGAGTCGCTTTGCGAGAAAATTGCCTGA

Upstream 100 bases:

>100_bases
TCGTCACCGGCATCGACGGCTGCTACTACAACGTCGTCGGCCTGCCGGTCTCGAAAGTGTACGCCGCGCTCAAACCACTC
TTCCCACTGGACGCGAATCG

Downstream 100 bases:

>100_bases
TCTTTTACCACATTCACCTGCCTCTACGCAATAAAGCTCTGAACGCACCCACCTCCCCTCTCAACTCCCACCGATATTTT
ACGCTATCGCAAAAAATATG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSQKPVLVILGPTASGKTELAFRIARKTGGEIISADSRQIYRSMDIGTAKPPKWMLNEVKHHFIDEKEIGEPFSAGDFAE
QAAERIRELRQRGITPIVAGGSTLYLEGLLKGFAELPQSNPEIRARLKHELELHGAEALYRRLEAFDPEQAKTLDPTKTQ
RLIRSLEIIEISGTTVTALQRKTSGPPPGIEFTVIALDLPREVLYERINRRTSEMMHAGLEAETRHLFDKFRDEWRSKKL
NALATVGYRELFEHFEGLYNLETAEALIAQHTRNYAKRQLTFFRNRLDVEWVKGPLDEAGIEALVESLCEKIA

Sequences:

>Translated_313_residues
MSQKPVLVILGPTASGKTELAFRIARKTGGEIISADSRQIYRSMDIGTAKPPKWMLNEVKHHFIDEKEIGEPFSAGDFAE
QAAERIRELRQRGITPIVAGGSTLYLEGLLKGFAELPQSNPEIRARLKHELELHGAEALYRRLEAFDPEQAKTLDPTKTQ
RLIRSLEIIEISGTTVTALQRKTSGPPPGIEFTVIALDLPREVLYERINRRTSEMMHAGLEAETRHLFDKFRDEWRSKKL
NALATVGYRELFEHFEGLYNLETAEALIAQHTRNYAKRQLTFFRNRLDVEWVKGPLDEAGIEALVESLCEKIA
>Mature_312_residues
SQKPVLVILGPTASGKTELAFRIARKTGGEIISADSRQIYRSMDIGTAKPPKWMLNEVKHHFIDEKEIGEPFSAGDFAEQ
AAERIRELRQRGITPIVAGGSTLYLEGLLKGFAELPQSNPEIRARLKHELELHGAEALYRRLEAFDPEQAKTLDPTKTQR
LIRSLEIIEISGTTVTALQRKTSGPPPGIEFTVIALDLPREVLYERINRRTSEMMHAGLEAETRHLFDKFRDEWRSKKLN
ALATVGYRELFEHFEGLYNLETAEALIAQHTRNYAKRQLTFFRNRLDVEWVKGPLDEAGIEALVESLCEKIA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=318, Percent_Identity=30.188679245283, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1790613, Length=286, Percent_Identity=35.6643356643357, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=317, Percent_Identity=26.1829652996845, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=301, Percent_Identity=27.5747508305648, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=30.3703703703704, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLP8 (B3QNZ0)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998843.1
- GeneID:   6420169
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1238
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK637545
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35505; Mature: 35374

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKPVLVILGPTASGKTELAFRIARKTGGEIISADSRQIYRSMDIGTAKPPKWMLNEVK
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCHHHHHHHHHCCCCCCCHHHHHHHHH
HHFIDEKEIGEPFSAGDFAEQAAERIRELRQRGITPIVAGGSTLYLEGLLKGFAELPQSN
HHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
PEIRARLKHELELHGAEALYRRLEAFDPEQAKTLDPTKTQRLIRSLEIIEISGTTVTALQ
HHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEEECCCEEEHHH
RKTSGPPPGIEFTVIALDLPREVLYERINRRTSEMMHAGLEAETRHLFDKFRDEWRSKKL
HHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
NALATVGYRELFEHFEGLYNLETAEALIAQHTRNYAKRQLTFFRNRLDVEWVKGPLDEAG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHH
IEALVESLCEKIA
HHHHHHHHHHHCC
>Mature Secondary Structure 
SQKPVLVILGPTASGKTELAFRIARKTGGEIISADSRQIYRSMDIGTAKPPKWMLNEVK
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCHHHHHHHHHCCCCCCCHHHHHHHHH
HHFIDEKEIGEPFSAGDFAEQAAERIRELRQRGITPIVAGGSTLYLEGLLKGFAELPQSN
HHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
PEIRARLKHELELHGAEALYRRLEAFDPEQAKTLDPTKTQRLIRSLEIIEISGTTVTALQ
HHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEEECCCEEEHHH
RKTSGPPPGIEFTVIALDLPREVLYERINRRTSEMMHAGLEAETRHLFDKFRDEWRSKKL
HHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
NALATVGYRELFEHFEGLYNLETAEALIAQHTRNYAKRQLTFFRNRLDVEWVKGPLDEAG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHH
IEALVESLCEKIA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA