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The map label for this gene is amtB [C]

Identifier: 193212877

GI number: 193212877

Start: 1339478

End: 1340815

Strand: Direct

Name: amtB [C]

Synonym: Cpar_1225

Alternate gene names: 193212877

Gene position: 1339478-1340815 (Clockwise)

Preceding gene: 193212874

Following gene: 193212878

Centisome position: 58.51

GC content: 56.5

Gene sequence:

>1338_bases
ATGAAATCATTTCTGTCGAAATCCGGGGCAATTGCTGCCGGACTGCTTGTAGCAGCGGCAATCTTGAGCCCTCCGCTCCT
GGCGGAAACGGCCGCAGCCGCGCCGACACCGGACGCTGTCAATGCCTTCGCCATCGACAACTTCTTCCTGTTTATTTGCG
CCGTGCTGGTGCTGTTCATGCAGGCGGGATTTGCTTTGGTTGAAACAGGCCTGAACGCCGCCAAAAACACCGTGAACATC
CTGTTCAAGAACCTGATGGATATGGCCATCGGCGCTATCCTCTTCTACTTCATCGGATACGGAATGATGTACCCCGGTGA
TGCCTTCAGCGGCGGCTTCTTTGGTTTCGGCAGCTGGGGAATCACTACTGACATGCCAGATATTGCAGCTGGCAGCCTCT
ATCCTGCGGTGGACTTCCTCTTCCAGGTCGCCTTTGCCGCAACAGCCGCAACGATCGTCTCGGGAGCGGTTGCCGGAAGG
ATGCAGTTCAGGTCTTATCTGATCTATTCAGCAGTCATTTCAGGACTTGTCTATCCCATTAGTGGCTTCTGGCAGTGGGG
TGGCGGCTGGTTGAACGCCATGGGCTTCCACGATTTTGCAGGTTCGCTTGTGGTGCACGCTCTCGGCGGTTTTGCCGGTC
TTGCCGGTGCCATCGTACTCGGCCCCCGTCTTGGCCGCTTCAACGAAGATGGTTCGCCGAACGCCATGCCTGGCCACAAC
CTCGCACTCAGCACCCTTGGTGTGTTCATTCTTCTTGTCGGCTGGTACGGCTTCAACCCCGGCAGCCAGCTTGCCATTGT
CGGCGGTGACAACACGGCTGCCGTCATGAAGATTGCTGTCAACACCACCCTTGCCGCCTGCGCTGGCGCGGTTGTTGCCA
TGATCTTCGCCTGGGGCCTCTTCAAGAAGCCAGACCTTACCATGGCTCTGAACGGTATGCTTGCCGGTTTGGTCGCCATC
ACGGCAAACTGCGACGTGGTTTCCTATAATGCTTCGCTGATTATCGGTGCAGTCGGTGGTGTTCTTGTCGTGCTCGGCAT
CATGCTGCTCGACAAGCTCCGTATCGACGACCCGGTCGGCGCATGGCCGGTACATGGCCTGAACGGCATCTGGGGTGGTA
TCGCCGCCTGGATTTTCGGTGGCCAGCCTATGGTTGCCCAGATCACCGGTTCGCTCGTGATCCCGGCCTGGGGTTTCGGC
ACCATGCTGGTCCTGTTCCTCATCCTGAAAGCTATGGGTATCCTGCGCGTTCACAAGGATGAAGAAATGAAAGGCCTCGA
TATTTCAGAACATGAAGAAGAAGCTTATTATGGCTTCGATATCTACACAACCCAGTAA

Upstream 100 bases:

>100_bases
CATTTAAAATTTATATTGCACCTGTGTGATGAGCTAATAATTCAACGCAAAAGAGCACACTCAGCTTTATTTTTTTCACA
ACCGTAACCTTTCAACAATC

Downstream 100 bases:

>100_bases
CGCATTTAAACGGAGCCTAAGCAATGAAATACATTGTAGCAATGATCCAGCCCCACAAGCTGTCGGACGTGAAAAAAAGC
CTTGCCGACGCCGGGGTTCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKSFLSKSGAIAAGLLVAAAILSPPLLAETAAAAPTPDAVNAFAIDNFFLFICAVLVLFMQAGFALVETGLNAAKNTVNI
LFKNLMDMAIGAILFYFIGYGMMYPGDAFSGGFFGFGSWGITTDMPDIAAGSLYPAVDFLFQVAFAATAATIVSGAVAGR
MQFRSYLIYSAVISGLVYPISGFWQWGGGWLNAMGFHDFAGSLVVHALGGFAGLAGAIVLGPRLGRFNEDGSPNAMPGHN
LALSTLGVFILLVGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKPDLTMALNGMLAGLVAI
TANCDVVSYNASLIIGAVGGVLVVLGIMLLDKLRIDDPVGAWPVHGLNGIWGGIAAWIFGGQPMVAQITGSLVIPAWGFG
TMLVLFLILKAMGILRVHKDEEMKGLDISEHEEEAYYGFDIYTTQ

Sequences:

>Translated_445_residues
MKSFLSKSGAIAAGLLVAAAILSPPLLAETAAAAPTPDAVNAFAIDNFFLFICAVLVLFMQAGFALVETGLNAAKNTVNI
LFKNLMDMAIGAILFYFIGYGMMYPGDAFSGGFFGFGSWGITTDMPDIAAGSLYPAVDFLFQVAFAATAATIVSGAVAGR
MQFRSYLIYSAVISGLVYPISGFWQWGGGWLNAMGFHDFAGSLVVHALGGFAGLAGAIVLGPRLGRFNEDGSPNAMPGHN
LALSTLGVFILLVGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKPDLTMALNGMLAGLVAI
TANCDVVSYNASLIIGAVGGVLVVLGIMLLDKLRIDDPVGAWPVHGLNGIWGGIAAWIFGGQPMVAQITGSLVIPAWGFG
TMLVLFLILKAMGILRVHKDEEMKGLDISEHEEEAYYGFDIYTTQ
>Mature_445_residues
MKSFLSKSGAIAAGLLVAAAILSPPLLAETAAAAPTPDAVNAFAIDNFFLFICAVLVLFMQAGFALVETGLNAAKNTVNI
LFKNLMDMAIGAILFYFIGYGMMYPGDAFSGGFFGFGSWGITTDMPDIAAGSLYPAVDFLFQVAFAATAATIVSGAVAGR
MQFRSYLIYSAVISGLVYPISGFWQWGGGWLNAMGFHDFAGSLVVHALGGFAGLAGAIVLGPRLGRFNEDGSPNAMPGHN
LALSTLGVFILLVGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKPDLTMALNGMLAGLVAI
TANCDVVSYNASLIIGAVGGVLVVLGIMLLDKLRIDDPVGAWPVHGLNGIWGGIAAWIFGGQPMVAQITGSLVIPAWGFG
TMLVLFLILKAMGILRVHKDEEMKGLDISEHEEEAYYGFDIYTTQ

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=412, Percent_Identity=34.7087378640777, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17550180, Length=432, Percent_Identity=34.2592592592593, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=36.2586605080831, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=34.1176470588235, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI133931100, Length=423, Percent_Identity=32.6241134751773, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17563228, Length=257, Percent_Identity=28.4046692607004, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325396, Length=415, Percent_Identity=28.6746987951807, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=432, Percent_Identity=27.0833333333333, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=27.4463007159904, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161078314, Length=409, Percent_Identity=34.4743276283619, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46504; Mature: 46504

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFLSKSGAIAAGLLVAAAILSPPLLAETAAAAPTPDAVNAFAIDNFFLFICAVLVLFM
CCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QAGFALVETGLNAAKNTVNILFKNLMDMAIGAILFYFIGYGMMYPGDAFSGGFFGFGSWG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ITTDMPDIAAGSLYPAVDFLFQVAFAATAATIVSGAVAGRMQFRSYLIYSAVISGLVYPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGFWQWGGGWLNAMGFHDFAGSLVVHALGGFAGLAGAIVLGPRLGRFNEDGSPNAMPGHN
CHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LALSTLGVFILLVGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGL
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHC
FKKPDLTMALNGMLAGLVAITANCDVVSYNASLIIGAVGGVLVVLGIMLLDKLRIDDPVG
CCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHCCCCCCC
AWPVHGLNGIWGGIAAWIFGGQPMVAQITGSLVIPAWGFGTMLVLFLILKAMGILRVHKD
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHEECCC
EEMKGLDISEHEEEAYYGFDIYTTQ
CCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKSFLSKSGAIAAGLLVAAAILSPPLLAETAAAAPTPDAVNAFAIDNFFLFICAVLVLFM
CCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QAGFALVETGLNAAKNTVNILFKNLMDMAIGAILFYFIGYGMMYPGDAFSGGFFGFGSWG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ITTDMPDIAAGSLYPAVDFLFQVAFAATAATIVSGAVAGRMQFRSYLIYSAVISGLVYPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGFWQWGGGWLNAMGFHDFAGSLVVHALGGFAGLAGAIVLGPRLGRFNEDGSPNAMPGHN
CHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LALSTLGVFILLVGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGL
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHC
FKKPDLTMALNGMLAGLVAITANCDVVSYNASLIIGAVGGVLVVLGIMLLDKLRIDDPVG
CCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHCCCCCCC
AWPVHGLNGIWGGIAAWIFGGQPMVAQITGSLVIPAWGFGTMLVLFLILKAMGILRVHKD
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHEECCC
EEMKGLDISEHEEEAYYGFDIYTTQ
CCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]