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The map label for this gene is rlmN [H]

Identifier: 192288832

GI number: 192288832

Start: 429106

End: 430305

Strand: Reverse

Name: rlmN [H]

Synonym: Rpal_0401

Alternate gene names: 192288832

Gene position: 430305-429106 (Counterclockwise)

Preceding gene: 192288833

Following gene: 192288831

Centisome position: 7.49

GC content: 65.0

Gene sequence:

>1200_bases
ATGACGCCTTCTGCAGCCTCGGCCCTGGTCGAGAAGACCCCGCTGGAAACCTATGTGCCGCCGGCCAAGCCGTCGCTGAT
CGGGCTGTCGCGGGCGCAATTGTGCGACCGGCTCGGCGACGTCGGGGTGGCGCCGCCCCAGCGCAAGATGCGGGCGCAGC
AGCTCTGGCACTGGATGTATGTCCGCGGCGCCCGTGACTTCTCCGAAATGACCAATGTCTCCAAGGAGATGCGGGCAACG
CTGGCGGAGCACTTCACGGTCGACCGGCCCGAAGTTGTGGCCGAGCAGATTTCGGCCGACGGCACCCGCAAATGGCTGCT
GCGGCTGCCGAGCGGCGGCGACGGCCAAAAGGCTCACGAGGTCGAGTGCGTCTATATCCCGGAGACCGACCGCGGCACGC
TGTGCGTCTCCAGCCAGGTCGGCTGCACCCTGAATTGCGCGTTCTGTCACACCGGCACCCAGCGCCTGGTGCGCAACCTC
ACGGCCGGCGAGATCGTCGGCCAAGTGATGGTGGCGCGCGACCGGCTGGGTGACTGGATCGACCGCGAGACACCGAACGG
CAACCGCCTGATCACCAACATCGTGATGATGGGGATGGGCGAGCCGCTGTACAATTTCGATGCGGTGCGCGACGCGCTGC
TGATCGTCTCCGACAATGAAGGCATCGGCATCTCGCGGCGGCGGATCACGCTGTCGACCTCCGGCGTGGTGCCGAACATC
AAGCGCACCGGCGACGAGATCGGCGTGATGCTGGCGATTTCGCTGCACGCGGTGCGCGACGAACTCCGCGACGAGCTGGT
GCCGCTGAACCGCAAATATCCGCTGAAGGAATTGCTACAGGCCTGCCGCGACTATCCGGGCGCGTCCAACGCTCGCCGCA
TCACCTTCGAATACGTGATGCTGAAGGGCGTCAACGACTCGCTCGACGATGCGCGCAAGCTGGTACAGTTGCTCAAGGGG
ATTCCGGCCAAGATCAATCTGATCCCGTTCAATCCATGGCCGGGCTCGAACTACGAGTGCTCGGACTGGGACCAGATCGA
GAAGTTCTCGGAATACGTTTTCAACGCCGGCTACTCGTCGCCGGTGCGCACCCCGCGCGGCCGCGACATTCTGGCGGCCT
GCGGCCAGCTCAAATCCGAAACCGAAAAGCTCTCGGTGCGCGAACGCAACGCGCTCCGCGCGATGGCGATGACGGACTGA

Upstream 100 bases:

>100_bases
AACCGGGCGGCGGGTTCTTATATGCATGGCGACCGGCGGCTTGTGCCCCGCTCTGCACGCCCGGCGCGGATTGGCCGGCC
ACCAACCTGAACGTGATGCG

Downstream 100 bases:

>100_bases
TCCAATGCTGCCCGCCAACGAGATCAAGGGCGTGCTCGCCTTCCTGCGGGAGGCCGAGCGGCTCAAGAGCGTGATCCGCA
GCGGGTACACGTCAACCGGC

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTPSAASALVEKTPLETYVPPAKPSLIGLSRAQLCDRLGDVGVAPPQRKMRAQQLWHWMYVRGARDFSEMTNVSKEMRAT
LAEHFTVDRPEVVAEQISADGTRKWLLRLPSGGDGQKAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNL
TAGEIVGQVMVARDRLGDWIDRETPNGNRLITNIVMMGMGEPLYNFDAVRDALLIVSDNEGIGISRRRITLSTSGVVPNI
KRTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARKLVQLLKG
IPAKINLIPFNPWPGSNYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRERNALRAMAMTD

Sequences:

>Translated_399_residues
MTPSAASALVEKTPLETYVPPAKPSLIGLSRAQLCDRLGDVGVAPPQRKMRAQQLWHWMYVRGARDFSEMTNVSKEMRAT
LAEHFTVDRPEVVAEQISADGTRKWLLRLPSGGDGQKAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNL
TAGEIVGQVMVARDRLGDWIDRETPNGNRLITNIVMMGMGEPLYNFDAVRDALLIVSDNEGIGISRRRITLSTSGVVPNI
KRTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARKLVQLLKG
IPAKINLIPFNPWPGSNYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRERNALRAMAMTD
>Mature_398_residues
TPSAASALVEKTPLETYVPPAKPSLIGLSRAQLCDRLGDVGVAPPQRKMRAQQLWHWMYVRGARDFSEMTNVSKEMRATL
AEHFTVDRPEVVAEQISADGTRKWLLRLPSGGDGQKAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLT
AGEIVGQVMVARDRLGDWIDRETPNGNRLITNIVMMGMGEPLYNFDAVRDALLIVSDNEGIGISRRRITLSTSGVVPNIK
RTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARKLVQLLKGI
PAKINLIPFNPWPGSNYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRERNALRAMAMTD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=382, Percent_Identity=47.3821989528796, Blast_Score=320, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 44436; Mature: 44304

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSAASALVEKTPLETYVPPAKPSLIGLSRAQLCDRLGDVGVAPPQRKMRAQQLWHWMY
CCCHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VRGARDFSEMTNVSKEMRATLAEHFTVDRPEVVAEQISADGTRKWLLRLPSGGDGQKAHE
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCE
VECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLTAGEIVGQVMVARDRLGDWI
EEEEEECCCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DRETPNGNRLITNIVMMGMGEPLYNFDAVRDALLIVSDNEGIGISRRRITLSTSGVVPNI
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCCCEEEEEEEECCCCCCCH
KRTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVM
HHCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
LKGVNDSLDDARKLVQLLKGIPAKINLIPFNPWPGSNYECSDWDQIEKFSEYVFNAGYSS
EECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PVRTPRGRDILAACGQLKSETEKLSVRERNALRAMAMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPSAASALVEKTPLETYVPPAKPSLIGLSRAQLCDRLGDVGVAPPQRKMRAQQLWHWMY
CCHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VRGARDFSEMTNVSKEMRATLAEHFTVDRPEVVAEQISADGTRKWLLRLPSGGDGQKAHE
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCE
VECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLTAGEIVGQVMVARDRLGDWI
EEEEEECCCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DRETPNGNRLITNIVMMGMGEPLYNFDAVRDALLIVSDNEGIGISRRRITLSTSGVVPNI
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCCCEEEEEEEECCCCCCCH
KRTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVM
HHCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
LKGVNDSLDDARKLVQLLKGIPAKINLIPFNPWPGSNYECSDWDQIEKFSEYVFNAGYSS
EECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PVRTPRGRDILAACGQLKSETEKLSVRERNALRAMAMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA