BacMap

Definition	Rhizobium etli CIAT 652 plasmid pC, complete sequence.
Accession	NC_010997
Length	1,091,523

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The map label for this gene is braF [H]

Identifier: 190894957

GI number: 190894957

Start: 675541

End: 676311

Strand: Reverse

Name: braF [H]

Synonym: RHECIAT_PC0000622

Alternate gene names: 190894957

Gene position: 676311-675541 (Counterclockwise)

Preceding gene: 190894958

Following gene: 190894956

Centisome position: 61.96

GC content: 61.22

Gene sequence:

>771_bases
ATGACCGGTCTTCCCATTCTCGAAATCAGCAATCTCACGAAATCCTACGGCGCGGTGAAGGCGGTCGACGATGTCAGCAT
TCATGTCGAGCGCGGCGAGATCGCCGGTTTGATCGGGCCGAACGGCTCGGGCAAATCGACCTTCTTCGATTGCAGCACCG
GCCTCGGCAAACCCGATAGCGGCAAGGTGAGGCTCGACGGCCAGGATATCACCGGATGGACGCTGAACCGGATCGCCCGC
GAGGGGCGCATGCTGCGCTCCTTCCAGAAGACGGTCGTCTTCAAATCGCTCGACGTCGAGGAAAACCTGATCATCGCGGG
GCAGATGTTCAGCTTCCCGTCGCTGTTTTCCACCTTCGGGATCGGCAAGCTGTCGCGCAGCCGGGTCTCAGGATTGCGCG
AACGTGCGCAGGAACTGATCAGGATGGCCGGGTTGTGGGACGTGCGCAAACAGCCGGCCGGCAATCTTTCCGGCGGGCAG
CAGAAGCTCATCCAGTTCGCTTCCATGCTGATGCCGGAGCCGAAGCTGATCCTGCTCGACGAGCCGATGGCGGGCATCAA
CCCGAAGATCATCGAGCGGGTGGTCGAGACCATTCTCTACGCCAACAAATCGCTCGGCGTCAGTTTCCTGATCATCGAAC
ACAATATCGACGTGGTGACCAGCATCTGCCGGCGGGTGATCGTGCTCGACCAGGGGCGCAAGCTCGCCGAGGGCCTGCCG
CAGGAAATCATCAACGACCAGCGGGTCAGGGAGGCCTATCTCGGTGGCTGA

Upstream 100 bases:

>100_bases
TGATCGCCTACGGGCTCTGCCTGCTCGTCTTGGTTTTTTGGTTCCGGAAGGGTTTTGCGCCCGCTATCAACCGCCTCTGG
GATTCGATCGGGAGCGCGAA

Downstream 100 bases:

>100_bases
TTCAAATCTCTCGATCAAGAGCCTGCGTGCCGGATATGGATCTCTCGATATCCTGAACGGCGTCGATCTCGACGTGCCGG
CAGGCCAGTTCGTGGCACTG

Product: putative amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTGLPILEISNLTKSYGAVKAVDDVSIHVERGEIAGLIGPNGSGKSTFFDCSTGLGKPDSGKVRLDGQDITGWTLNRIAR
EGRMLRSFQKTVVFKSLDVEENLIIAGQMFSFPSLFSTFGIGKLSRSRVSGLRERAQELIRMAGLWDVRKQPAGNLSGGQ
QKLIQFASMLMPEPKLILLDEPMAGINPKIIERVVETILYANKSLGVSFLIIEHNIDVVTSICRRVIVLDQGRKLAEGLP
QEIINDQRVREAYLGG

Sequences:

>Translated_256_residues
MTGLPILEISNLTKSYGAVKAVDDVSIHVERGEIAGLIGPNGSGKSTFFDCSTGLGKPDSGKVRLDGQDITGWTLNRIAR
EGRMLRSFQKTVVFKSLDVEENLIIAGQMFSFPSLFSTFGIGKLSRSRVSGLRERAQELIRMAGLWDVRKQPAGNLSGGQ
QKLIQFASMLMPEPKLILLDEPMAGINPKIIERVVETILYANKSLGVSFLIIEHNIDVVTSICRRVIVLDQGRKLAEGLP
QEIINDQRVREAYLGG
>Mature_255_residues
TGLPILEISNLTKSYGAVKAVDDVSIHVERGEIAGLIGPNGSGKSTFFDCSTGLGKPDSGKVRLDGQDITGWTLNRIARE
GRMLRSFQKTVVFKSLDVEENLIIAGQMFSFPSLFSTFGIGKLSRSRVSGLRERAQELIRMAGLWDVRKQPAGNLSGGQQ
KLIQFASMLMPEPKLILLDEPMAGINPKIIERVVETILYANKSLGVSFLIIEHNIDVVTSICRRVIVLDQGRKLAEGLPQ
EIINDQRVREAYLGG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=221, Percent_Identity=27.1493212669683, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI30795238, Length=221, Percent_Identity=27.1493212669683, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI105990541, Length=239, Percent_Identity=27.1966527196653, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI47078218, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI45446740, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI150417984, Length=245, Percent_Identity=27.3469387755102, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI116734710, Length=244, Percent_Identity=26.6393442622951, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI31657092, Length=237, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI171184400, Length=247, Percent_Identity=27.9352226720648, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI27436953, Length=257, Percent_Identity=26.0700389105058, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI21536376, Length=242, Percent_Identity=28.5123966942149, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27477115, Length=197, Percent_Identity=31.4720812182741, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27262624, Length=231, Percent_Identity=25.5411255411255, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27262626, Length=231, Percent_Identity=25.5411255411255, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=258, Percent_Identity=25.968992248062, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=35.4581673306773, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1789593, Length=257, Percent_Identity=33.4630350194552, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082268, Length=256, Percent_Identity=31.25, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI87081791, Length=248, Percent_Identity=30.6451612903226, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786398, Length=249, Percent_Identity=28.1124497991968, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790544, Length=253, Percent_Identity=29.2490118577075, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787370, Length=245, Percent_Identity=25.3061224489796, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081709, Length=243, Percent_Identity=25.1028806584362, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788761, Length=244, Percent_Identity=24.5901639344262, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787029, Length=251, Percent_Identity=27.4900398406374, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788450, Length=236, Percent_Identity=28.3898305084746, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081782, Length=248, Percent_Identity=32.258064516129, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789672, Length=243, Percent_Identity=27.9835390946502, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787712, Length=250, Percent_Identity=28, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786253, Length=251, Percent_Identity=25.8964143426295, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788225, Length=244, Percent_Identity=26.2295081967213, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786872, Length=242, Percent_Identity=28.099173553719, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994943, Length=252, Percent_Identity=26.984126984127, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1789962, Length=259, Percent_Identity=28.1853281853282, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787500, Length=231, Percent_Identity=30.7359307359307, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=27.5423728813559, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48995001, Length=228, Percent_Identity=29.8245614035088, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790739, Length=250, Percent_Identity=28, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790525, Length=246, Percent_Identity=26.0162601626016, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786803, Length=243, Percent_Identity=25.5144032921811, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786319, Length=227, Percent_Identity=26.431718061674, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1788897, Length=236, Percent_Identity=27.1186440677966, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789586, Length=257, Percent_Identity=27.2373540856031, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787105, Length=234, Percent_Identity=29.4871794871795, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789991, Length=232, Percent_Identity=27.5862068965517, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786563, Length=236, Percent_Identity=26.271186440678, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788644, Length=249, Percent_Identity=28.5140562248996, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI48994997, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790467, Length=242, Percent_Identity=26.4462809917355, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786345, Length=232, Percent_Identity=24.5689655172414, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081834, Length=238, Percent_Identity=27.3109243697479, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789032, Length=225, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789873, Length=238, Percent_Identity=28.1512605042017, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1787792, Length=244, Percent_Identity=25, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790162, Length=219, Percent_Identity=26.4840182648402, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082267, Length=238, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790190, Length=232, Percent_Identity=25.4310344827586, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787547, Length=243, Percent_Identity=25.5144032921811, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786703, Length=239, Percent_Identity=26.3598326359833, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789963, Length=267, Percent_Identity=25.4681647940075, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693107, Length=243, Percent_Identity=27.9835390946502, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787164, Length=232, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788210, Length=251, Percent_Identity=23.5059760956175, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI2367384, Length=63, Percent_Identity=50.7936507936508, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI48994883, Length=227, Percent_Identity=24.2290748898678, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788506, Length=242, Percent_Identity=28.099173553719, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787041, Length=232, Percent_Identity=25.4310344827586, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=238, Percent_Identity=27.3109243697479, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71983516, Length=241, Percent_Identity=28.2157676348548, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71983510, Length=241, Percent_Identity=28.2157676348548, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71983523, Length=241, Percent_Identity=28.2157676348548, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17565586, Length=236, Percent_Identity=24.5762711864407, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=235, Percent_Identity=25.9574468085106, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193211017, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=220, Percent_Identity=26.8181818181818, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17567265, Length=237, Percent_Identity=24.8945147679325, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115534520, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI212646699, Length=246, Percent_Identity=25.2032520325203, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115535101, Length=237, Percent_Identity=25.3164556962025, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI86563255, Length=239, Percent_Identity=24.2677824267782, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221512771, Length=233, Percent_Identity=29.1845493562232, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666092, Length=227, Percent_Identity=26.8722466960352, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28574150, Length=230, Percent_Identity=26.5217391304348, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI116007328, Length=230, Percent_Identity=26.5217391304348, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24650855, Length=237, Percent_Identity=26.1603375527426, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650853, Length=237, Percent_Identity=26.1603375527426, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281362751, Length=244, Percent_Identity=25.4098360655738, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI85725262, Length=244, Percent_Identity=26.6393442622951, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582014, Length=262, Percent_Identity=24.0458015267176, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582012, Length=262, Percent_Identity=24.0458015267176, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648314, Length=187, Percent_Identity=28.3422459893048, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007184, Length=227, Percent_Identity=24.6696035242291, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221500365, Length=227, Percent_Identity=24.6696035242291, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643674, Length=240, Percent_Identity=25.4166666666667, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571209, Length=245, Percent_Identity=24.4897959183673, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363262, Length=232, Percent_Identity=24.5689655172414, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24653245, Length=232, Percent_Identity=24.5689655172414, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28097; Mature: 27966

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLPILEISNLTKSYGAVKAVDDVSIHVERGEIAGLIGPNGSGKSTFFDCSTGLGKPDS
CCCCCEEEHHHHHHHHCCEEEECCEEEEEECCCEEEEECCCCCCCCEEEECCCCCCCCCC
GKVRLDGQDITGWTLNRIAREGRMLRSFQKTVVFKSLDVEENLIIAGQMFSFPSLFSTFG
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHC
IGKLSRSRVSGLRERAQELIRMAGLWDVRKQPAGNLSGGQQKLIQFASMLMPEPKLILLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
EPMAGINPKIIERVVETILYANKSLGVSFLIIEHNIDVVTSICRRVIVLDQGRKLAEGLP
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHCCC
QEIINDQRVREAYLGG
HHHHCHHHHHHHHCCC
>Mature Secondary Structure 
TGLPILEISNLTKSYGAVKAVDDVSIHVERGEIAGLIGPNGSGKSTFFDCSTGLGKPDS
CCCCEEEHHHHHHHHCCEEEECCEEEEEECCCEEEEECCCCCCCCEEEECCCCCCCCCC
GKVRLDGQDITGWTLNRIAREGRMLRSFQKTVVFKSLDVEENLIIAGQMFSFPSLFSTFG
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHC
IGKLSRSRVSGLRERAQELIRMAGLWDVRKQPAGNLSGGQQKLIQFASMLMPEPKLILLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
EPMAGINPKIIERVVETILYANKSLGVSFLIIEHNIDVVTSICRRVIVLDQGRKLAEGLP
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHCCC
QEIINDQRVREAYLGG
HHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]