Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 190571728

GI number: 190571728

Start: 1449707

End: 1450813

Strand: Reverse

Name: dacF [H]

Synonym: WPa_1357

Alternate gene names: 190571728

Gene position: 1450813-1449707 (Counterclockwise)

Preceding gene: 190571730

Following gene: 190571724

Centisome position: 97.87

GC content: 32.61

Gene sequence:

>1107_bases
ATGTTAAGCAAATTAGTAACTTTGCTATTAATTTTTATATTTCCTTTTTACTCATACTCATATCAGTTTAGAACCAAAGC
AAAGCAAGCAATTGTCTTAGATTTAGCTTCAGACTCGTTCATTTTTGAACACAATTCCAGGGAAAAAATGGCCCCATCTT
CAATGAGTAAGTTAATGACTTTATATGTAGCTTTTGACTATCTAAAAGCTGGAATAATAAAAATGGAAGATAAATTTCGA
GTAAGTAGAAAAGCATGGGAAAGGAAAGGCTCTTCTATGTTTTTGAAAGAAGGTCAATTTGTTACGGTAAAAGAACTACT
TGAAGGAATTACAATTGCCTCAGGTAATGATGCATGCATAGCGCTAGCCGAGGGTATCGCAGGATCAGAAGAGAATTTTG
TTGCTGAAATGAATGAAGTTGCACAAGATTTAAATCTTAATGATAGTCATTTTGTTAACTCAAGCGGGTGGCCAGATGAA
AACCATTTTATGAGCGCAAAAGACTTGGTAACACTGGCAAAAAGGATTTTTACTGATTTTCCTGAATACTATGATCTATT
CTCTGAACAATATTTGACATATAACGATATTGTACAAAAAAATAAAAATCTTTTACTTTTCCATGATATTGGAGTTGATG
GTCTAAAGACAGGTCATACAAGTGCTGGTGGTTATGGAATTATAGTATCTGCAAAACGGAACGATAGGAGAATTTTTGCT
GTTGTGAATGGTTTGGATACCGAGAAAGAGCGAATAGAGGAAGCAAAAAGACTAATACAATATTCCTTAAATCATTTTAA
TACCAAGAAAATATTTGCTAAGGATAGCGTAGTTGAAGAAATAAATATTGTTTATGGAAAAGATAAAAAGGTGCCCGTTA
CAGTTGCAAACGATGTTACCATAACTTATAACCGCAGATTACATGATAAAATTAAAGTGCGTATTGAGTATAAAGATATG
GTACCCGCACCAATTAAAAAAGGTCAAGAAGTGGGCAAAATTTTTGTAGAAATACCTGGTACTGAGCAACAAACTATACC
TGTTTATACAGCAAATGATGTGCAGAAATTGAGCTATATAAAAAAAATTTTTAGAAAGTTGTTTTAA

Upstream 100 bases:

>100_bases
TGGCGCAGTTTGTCAAGGAAGTTTTATGCGAGAGATCTATTGAAATGCTCGTGCAAAATCATACTATTAATTAGTACACT
ATAAATTTAAAATTATAAAT

Downstream 100 bases:

>100_bases
TCAGCAAATGTAAGTTAAGGAGACCTGAAATACTAATCTACTTTCAACCTCTCAGCATGTAAACTGCGTGGTAAAGATTC
CAACTCTTTTGCAGCAGTGA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLSKLVTLLLIFIFPFYSYSYQFRTKAKQAIVLDLASDSFIFEHNSREKMAPSSMSKLMTLYVAFDYLKAGIIKMEDKFR
VSRKAWERKGSSMFLKEGQFVTVKELLEGITIASGNDACIALAEGIAGSEENFVAEMNEVAQDLNLNDSHFVNSSGWPDE
NHFMSAKDLVTLAKRIFTDFPEYYDLFSEQYLTYNDIVQKNKNLLLFHDIGVDGLKTGHTSAGGYGIIVSAKRNDRRIFA
VVNGLDTEKERIEEAKRLIQYSLNHFNTKKIFAKDSVVEEINIVYGKDKKVPVTVANDVTITYNRRLHDKIKVRIEYKDM
VPAPIKKGQEVGKIFVEIPGTEQQTIPVYTANDVQKLSYIKKIFRKLF

Sequences:

>Translated_368_residues
MLSKLVTLLLIFIFPFYSYSYQFRTKAKQAIVLDLASDSFIFEHNSREKMAPSSMSKLMTLYVAFDYLKAGIIKMEDKFR
VSRKAWERKGSSMFLKEGQFVTVKELLEGITIASGNDACIALAEGIAGSEENFVAEMNEVAQDLNLNDSHFVNSSGWPDE
NHFMSAKDLVTLAKRIFTDFPEYYDLFSEQYLTYNDIVQKNKNLLLFHDIGVDGLKTGHTSAGGYGIIVSAKRNDRRIFA
VVNGLDTEKERIEEAKRLIQYSLNHFNTKKIFAKDSVVEEINIVYGKDKKVPVTVANDVTITYNRRLHDKIKVRIEYKDM
VPAPIKKGQEVGKIFVEIPGTEQQTIPVYTANDVQKLSYIKKIFRKLF
>Mature_368_residues
MLSKLVTLLLIFIFPFYSYSYQFRTKAKQAIVLDLASDSFIFEHNSREKMAPSSMSKLMTLYVAFDYLKAGIIKMEDKFR
VSRKAWERKGSSMFLKEGQFVTVKELLEGITIASGNDACIALAEGIAGSEENFVAEMNEVAQDLNLNDSHFVNSSGWPDE
NHFMSAKDLVTLAKRIFTDFPEYYDLFSEQYLTYNDIVQKNKNLLLFHDIGVDGLKTGHTSAGGYGIIVSAKRNDRRIFA
VVNGLDTEKERIEEAKRLIQYSLNHFNTKKIFAKDSVVEEINIVYGKDKKVPVTVANDVTITYNRRLHDKIKVRIEYKDM
VPAPIKKGQEVGKIFVEIPGTEQQTIPVYTANDVQKLSYIKKIFRKLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=346, Percent_Identity=33.8150289017341, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082022, Length=317, Percent_Identity=37.2239747634069, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786851, Length=357, Percent_Identity=31.9327731092437, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42064; Mature: 42064

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKLVTLLLIFIFPFYSYSYQFRTKAKQAIVLDLASDSFIFEHNSREKMAPSSMSKLMT
CHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LYVAFDYLKAGIIKMEDKFRVSRKAWERKGSSMFLKEGQFVTVKELLEGITIASGNDACI
HHHHHHHHHHCCEEEHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHCCCEEECCCCCEE
ALAEGIAGSEENFVAEMNEVAQDLNLNDSHFVNSSGWPDENHFMSAKDLVTLAKRIFTDF
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
PEYYDLFSEQYLTYNDIVQKNKNLLLFHDIGVDGLKTGHTSAGGYGIIVSAKRNDRRIFA
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEE
VVNGLDTEKERIEEAKRLIQYSLNHFNTKKIFAKDSVVEEINIVYGKDKKVPVTVANDVT
EECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHEEEEECCCCCCEEEEECCEE
ITYNRRLHDKIKVRIEYKDMVPAPIKKGQEVGKIFVEIPGTEQQTIPVYTANDVQKLSYI
EEECCEECEEEEEEEEECCCCCCCHHCCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHH
KKIFRKLF
HHHHHHHC
>Mature Secondary Structure
MLSKLVTLLLIFIFPFYSYSYQFRTKAKQAIVLDLASDSFIFEHNSREKMAPSSMSKLMT
CHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LYVAFDYLKAGIIKMEDKFRVSRKAWERKGSSMFLKEGQFVTVKELLEGITIASGNDACI
HHHHHHHHHHCCEEEHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHCCCEEECCCCCEE
ALAEGIAGSEENFVAEMNEVAQDLNLNDSHFVNSSGWPDENHFMSAKDLVTLAKRIFTDF
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
PEYYDLFSEQYLTYNDIVQKNKNLLLFHDIGVDGLKTGHTSAGGYGIIVSAKRNDRRIFA
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEE
VVNGLDTEKERIEEAKRLIQYSLNHFNTKKIFAKDSVVEEINIVYGKDKKVPVTVANDVT
EECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHEEEEECCCCCCEEEEECCEE
ITYNRRLHDKIKVRIEYKDMVPAPIKKGQEVGKIFVEIPGTEQQTIPVYTANDVQKLSYI
EEECCEECEEEEEEEEECCCCCCCHHCCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHH
KKIFRKLF
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]