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The map label for this gene is pdhB [H]

Identifier: 190571649

GI number: 190571649

Start: 1348831

End: 1349829

Strand: Reverse

Name: pdhB [H]

Synonym: WPa_1274

Alternate gene names: 190571649

Gene position: 1349829-1348831 (Counterclockwise)

Preceding gene: 190571650

Following gene: 190571647

Centisome position: 91.05

GC content: 40.94

Gene sequence:

>999_bases
ATGGCAATCTTAAGTGTAAGAGAAGCTTTATGCACAGCGATCAGAGAAGAAATGCAAAATGACCCTGATATATTTATCAT
GGGTGAAGAAGTTGCAGAGTATGATGGTGCTTATAAAGTAACGAAAGGATTACTGAAAGAGTTTGGAGAAAATAGGGTAG
TTGATACACCCATTACTGAACATGGATTTGCTGGTCTTGCTGTTGGAGCAGCATTTGCTGGACTGAGGCCAATAGTCGAG
TTTATGACTTTTAATTTTTCTATGCAGGCTATCGACCAAATTGTGAATTCCGCAGCAAAAACAAATTATATGTCGGGTGG
ACAACTTGGATGCCCTATAGTATTTCGTGGACCAAATGGAGCTGCAGCAAGAGTTGCCGCACAACATTCTCAGTGCTTTG
CATCTTGGTATTCGCATGTTCCAGGTTTGAAAGTAATAGCACCTTACTTTGCCTCCGATTGCAGAGGTCTGCTTAAAGCT
GCAATTCGTGACCCTAATCCGGTAATATTTCTAGAAAACGAGATCGCTTATGGACATGAGCATGAAGTTCCTGACTCTGA
GCTATCAAATAAAGATTATCTACTTGAGATAGGCAAAGCTGCTGTTATACGGGAAGGAAAGGATGTAACTATCACTGCTT
TTTCATTGAAACTAATGGATGCGTTAAATGCGGCAGATTTACTCTCTAGTGAAGGAATAGAAGCTGAAGTTATCGACCTC
AGAACCTTAAGACCACTTGACACTGAAACAGTTATTAACTCTATTAAGAAGACCAATAGATTAGTAAGCATAGAAGAAGG
TTGGCCATTTGCAGGAATAGGAGCAGAACTCTCAGCCATGATTATGGAACAAGGATTTGACTACCTTGACGCTCCGGTTG
TACGGGTAACCGGCAAGGATGTCCCCTTACCTTATGCTGCAAACCTAGAAAAGAAAGCATTACCACAAGTGGAAGATATA
GTTGAAGCTGTGCACCAAGTCTGCTTTAGAAAGAAATAG

Upstream 100 bases:

>100_bases
TATTCAATGCTCAGTGCAACATGATGCTTGTAAAACTTAACTTGTGCGCTTCCTGGTAATTTTGATATAGTTATTTCAGT
TGAAATCTACAGGTTTACAA

Downstream 100 bases:

>100_bases
TCCTATTGACTCTTTTTTTTATCTCCCGTTCCACATTCTTCCATAAAAGAGGCATTTGTAGAGTTTATACCACCCATTAC
TTTTATGACAATAGTTTTAG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAILSVREALCTAIREEMQNDPDIFIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITEHGFAGLAVGAAFAGLRPIVE
FMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAARVAAQHSQCFASWYSHVPGLKVIAPYFASDCRGLLKA
AIRDPNPVIFLENEIAYGHEHEVPDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
RTLRPLDTETVINSIKKTNRLVSIEEGWPFAGIGAELSAMIMEQGFDYLDAPVVRVTGKDVPLPYAANLEKKALPQVEDI
VEAVHQVCFRKK

Sequences:

>Translated_332_residues
MAILSVREALCTAIREEMQNDPDIFIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITEHGFAGLAVGAAFAGLRPIVE
FMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAARVAAQHSQCFASWYSHVPGLKVIAPYFASDCRGLLKA
AIRDPNPVIFLENEIAYGHEHEVPDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
RTLRPLDTETVINSIKKTNRLVSIEEGWPFAGIGAELSAMIMEQGFDYLDAPVVRVTGKDVPLPYAANLEKKALPQVEDI
VEAVHQVCFRKK
>Mature_331_residues
AILSVREALCTAIREEMQNDPDIFIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITEHGFAGLAVGAAFAGLRPIVEF
MTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAARVAAQHSQCFASWYSHVPGLKVIAPYFASDCRGLLKAA
IRDPNPVIFLENEIAYGHEHEVPDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDLR
TLRPLDTETVINSIKKTNRLVSIEEGWPFAGIGAELSAMIMEQGFDYLDAPVVRVTGKDVPLPYAANLEKKALPQVEDIV
EAVHQVCFRKK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=58.641975308642, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=54.9382716049383, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI4557353, Length=304, Percent_Identity=35.1973684210526, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI34101272, Length=304, Percent_Identity=35.1973684210526, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI205277463, Length=292, Percent_Identity=28.0821917808219, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4507521, Length=292, Percent_Identity=28.0821917808219, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI133778974, Length=93, Percent_Identity=39.7849462365591, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=61.9631901840491, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17506935, Length=325, Percent_Identity=35.3846153846154, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=59.3272171253823, Blast_Score=401, Evalue=1e-113,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=61.8461538461539, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=61.8461538461539, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=34.3558282208589, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=34.3558282208589, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=65.5555555555556, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=65.5555555555556, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45551847, Length=274, Percent_Identity=27.7372262773723, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550715, Length=274, Percent_Identity=27.7372262773723, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24645119, Length=274, Percent_Identity=27.7372262773723, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36239; Mature: 36108

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILSVREALCTAIREEMQNDPDIFIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITE
CCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCEECCCCCC
HGFAGLAVGAAFAGLRPIVEFMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
AAARVAAQHSQCFASWYSHVPGLKVIAPYFASDCRGLLKAAIRDPNPVIFLENEIAYGHE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
HEVPDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
CCCCCCCCCCCCCEEECCCHHEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEH
RTLRPLDTETVINSIKKTNRLVSIEEGWPFAGIGAELSAMIMEQGFDYLDAPVVRVTGKD
HCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHCCCEEEECCCC
VPLPYAANLEKKALPQVEDIVEAVHQVCFRKK
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AILSVREALCTAIREEMQNDPDIFIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITE
CHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCEECCCCCC
HGFAGLAVGAAFAGLRPIVEFMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
AAARVAAQHSQCFASWYSHVPGLKVIAPYFASDCRGLLKAAIRDPNPVIFLENEIAYGHE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
HEVPDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
CCCCCCCCCCCCCEEECCCHHEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEH
RTLRPLDTETVINSIKKTNRLVSIEEGWPFAGIGAELSAMIMEQGFDYLDAPVVRVTGKD
HCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHCCCEEEECCCC
VPLPYAANLEKKALPQVEDIVEAVHQVCFRKK
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]