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The map label for this gene is bcr [H]

Identifier: 190571598

GI number: 190571598

Start: 1294157

End: 1295344

Strand: Reverse

Name: bcr [H]

Synonym: WPa_1221

Alternate gene names: 190571598

Gene position: 1295344-1294157 (Counterclockwise)

Preceding gene: 190571602

Following gene: 190571597

Centisome position: 87.38

GC content: 33.25

Gene sequence:

>1188_bases
GTGTCGACAGCTTTTTCACAAAATATCATCTCTCTTATTATGATATTATCTGTAGCAGTTGTTGATATGGCAACAGACTT
ATACTCAGTTGCATTGCCAAGTATTGCCAACTATTTTAAGGTGAAAGGTAATATAGCGCAGCTTACGATCAGTTTAAATT
TAGTAGGAGTTGCAATATCTGGATTAATTTATGGGCCACTGTCAGACTATTATGGTAGGCGCCTAATAATGCTGATTGGC
ATGGCAATTTTTACTTTGGCAAGTATTGTATGTTGCATAGCTGATAATATTATACTTTTAATATTAATCCGTTTTATACA
AGGAGCAGGAGCTGGTGTTGCAGGCGTTGTCGGATATGCAGCAATCAGGGATATGTACTCAGGTAGTGAATACTCAAGAG
TAATCTCAAAGTTAAATATGGTAGTAGCACTTTCACCTGGAATAGCTCCAGTGGTAGGCAGTTATATAATTTCACATGGT
TATAGTTGGAAGTTTTTGTTTTTTATCATATCACTTGCCGCAATTATTATGCTCATTTTTATTTACTTGAAGCTACAAGA
AACGCTGACCGTAAATAAAAGTAAAGCCAGTATTAATATCTTCAAGCAGTATATATTAATATTTAAGAATTATCGTTTCC
TTGGGTTTTCAGCCATTCATGGATTAACTTTCATGTGGCTTTGGGCATACATTGCTAACTACCCGTTCATATTTGAATCA
ATGGGCGTTGAAGTACAATATTTTGGCTATCTCATATCAATCATAGTTATATTTTACATAATTGGAACTTTAATTAATAG
AAGGTACGTACCAAAAATAGGAGTGAGCAAGATGCTAATCATAGGTTTGGTATTACCAATAATATCTGATGGTTTATTGG
TATATTTTTATTTCATGAACAAATTGAACGTACTTATTCTTCAAGTTCTTTGGATTCCAAGCAATATAGGACTTGCACTC
GTAATCAGCAATAACGTAACTTCTGCTTTAGAAACGGTCAAAAGTATAGGGCTTGGTAGTGCAGTTCTCTCATTTTGCAA
TATGATGTTTGGGGCTGTTGGAATATACATAGTAGGAAAATTCTTTTCTTATGGCATTTTACCAAATTTACTATTAACAG
TTGTATGTTCTATAATTGCAATTCTTATATATAGTTTACTTAAATATACTGAAAAACACGAAGGATAA

Upstream 100 bases:

>100_bases
GAGATTGCTTACATAATTATTTTAAACTTTAGCAGTTAAATTAATATACTAATGAGAAAAATATACTACAATTAAACATA
TTTAATCCAGTAAATCATTA

Downstream 100 bases:

>100_bases
TCATTATTTCTACAACCCAGATTTCATGAATTATTAATTACCTTACTATAGTAATGCAATTTTATAGAGAAGTGTTAAAT
GAATTATTCAATCAGAAATA

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSTAFSQNIISLIMILSVAVVDMATDLYSVALPSIANYFKVKGNIAQLTISLNLVGVAISGLIYGPLSDYYGRRLIMLIG
MAIFTLASIVCCIADNIILLILIRFIQGAGAGVAGVVGYAAIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHG
YSWKFLFFIISLAAIIMLIFIYLKLQETLTVNKSKASINIFKQYILIFKNYRFLGFSAIHGLTFMWLWAYIANYPFIFES
MGVEVQYFGYLISIIVIFYIIGTLINRRYVPKIGVSKMLIIGLVLPIISDGLLVYFYFMNKLNVLILQVLWIPSNIGLAL
VISNNVTSALETVKSIGLGSAVLSFCNMMFGAVGIYIVGKFFSYGILPNLLLTVVCSIIAILIYSLLKYTEKHEG

Sequences:

>Translated_395_residues
MSTAFSQNIISLIMILSVAVVDMATDLYSVALPSIANYFKVKGNIAQLTISLNLVGVAISGLIYGPLSDYYGRRLIMLIG
MAIFTLASIVCCIADNIILLILIRFIQGAGAGVAGVVGYAAIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHG
YSWKFLFFIISLAAIIMLIFIYLKLQETLTVNKSKASINIFKQYILIFKNYRFLGFSAIHGLTFMWLWAYIANYPFIFES
MGVEVQYFGYLISIIVIFYIIGTLINRRYVPKIGVSKMLIIGLVLPIISDGLLVYFYFMNKLNVLILQVLWIPSNIGLAL
VISNNVTSALETVKSIGLGSAVLSFCNMMFGAVGIYIVGKFFSYGILPNLLLTVVCSIIAILIYSLLKYTEKHEG
>Mature_394_residues
STAFSQNIISLIMILSVAVVDMATDLYSVALPSIANYFKVKGNIAQLTISLNLVGVAISGLIYGPLSDYYGRRLIMLIGM
AIFTLASIVCCIADNIILLILIRFIQGAGAGVAGVVGYAAIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHGY
SWKFLFFIISLAAIIMLIFIYLKLQETLTVNKSKASINIFKQYILIFKNYRFLGFSAIHGLTFMWLWAYIANYPFIFESM
GVEVQYFGYLISIIVIFYIIGTLINRRYVPKIGVSKMLIIGLVLPIISDGLLVYFYFMNKLNVLILQVLWIPSNIGLALV
ISNNVTSALETVKSIGLGSAVLSFCNMMFGAVGIYIVGKFFSYGILPNLLLTVVCSIIAILIYSLLKYTEKHEG

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=278, Percent_Identity=31.294964028777, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI48994889, Length=265, Percent_Identity=29.811320754717, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790146, Length=230, Percent_Identity=28.2608695652174, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787065, Length=221, Percent_Identity=30.316742081448, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790794, Length=415, Percent_Identity=23.6144578313253, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082312, Length=194, Percent_Identity=29.8969072164948, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081983, Length=195, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=183, Percent_Identity=29.5081967213115, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321838, Length=198, Percent_Identity=26.7676767676768, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43559; Mature: 43428

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAFSQNIISLIMILSVAVVDMATDLYSVALPSIANYFKVKGNIAQLTISLNLVGVAIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
GLIYGPLSDYYGRRLIMLIGMAIFTLASIVCCIADNIILLILIRFIQGAGAGVAGVVGYA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLAAIIMLIF
HHHHHHCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IYLKLQETLTVNKSKASINIFKQYILIFKNYRFLGFSAIHGLTFMWLWAYIANYPFIFES
HHHHHHHHHEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MGVEVQYFGYLISIIVIFYIIGTLINRRYVPKIGVSKMLIIGLVLPIISDGLLVYFYFMN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
KLNVLILQVLWIPSNIGLALVISNNVTSALETVKSIGLGSAVLSFCNMMFGAVGIYIVGK
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FFSYGILPNLLLTVVCSIIAILIYSLLKYTEKHEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STAFSQNIISLIMILSVAVVDMATDLYSVALPSIANYFKVKGNIAQLTISLNLVGVAIS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
GLIYGPLSDYYGRRLIMLIGMAIFTLASIVCCIADNIILLILIRFIQGAGAGVAGVVGYA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIRDMYSGSEYSRVISKLNMVVALSPGIAPVVGSYIISHGYSWKFLFFIISLAAIIMLIF
HHHHHHCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IYLKLQETLTVNKSKASINIFKQYILIFKNYRFLGFSAIHGLTFMWLWAYIANYPFIFES
HHHHHHHHHEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MGVEVQYFGYLISIIVIFYIIGTLINRRYVPKIGVSKMLIIGLVLPIISDGLLVYFYFMN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
KLNVLILQVLWIPSNIGLALVISNNVTSALETVKSIGLGSAVLSFCNMMFGAVGIYIVGK
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FFSYGILPNLLLTVVCSIIAILIYSLLKYTEKHEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]