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The map label for this gene is maeB [H]

Identifier: 190571588

GI number: 190571588

Start: 1284018

End: 1285340

Strand: Reverse

Name: maeB [H]

Synonym: WPa_1210

Alternate gene names: 190571588

Gene position: 1285340-1284018 (Counterclockwise)

Preceding gene: 190571596

Following gene: 190571587

Centisome position: 86.7

GC content: 40.29

Gene sequence:

>1323_bases
ATGAATAATGATTTAGATAACACCACAAAACAAGAAGCACTTAAGTACCATAATAAAAGTGGTAAACCTGGTAAGGTATC
AGTTATACCAACAAAACCCTTATCCACACAGCATGACCTATCACTTGCTTATTCCCCTGGTGTTGCAGCCCCATGCCTTG
CAATAGCTAAAAATCCTAAAGCTGTTTATGATTACACGGCAAAAAGCAATTATGTGGCTGTCATTTCAAATGGCACCGCA
GTGCTGGGACTTGGCAATATCGGTCCTCTTGCTTCAAAACCCGTCATGGAAGGCAAAGCTGTTTTATTTAAGCGTTTTGC
TGACATTGATGCAATTGATATAGAAGTTGACACAGAAGATATAGAAGATTTCATCAATGCAGTAAGGTATCTTGGTCCAA
GTTGGGGGGGAATAAATCTAGAAGATATAAGATCTCCTGATTGTTTTATAATAGAGAAACGTCTAAATGAGTTGATGGAT
ATTCCGGTGTTCCACGATGATCAACATGGCACTGCAGTAGTTGTTGCAGCTGGTATAGAAAATGCCCTCGATATTGCTGA
AAAGAAATTAGAGAATGTCAAGATCATCATGAATGGTTCTGGAGCAGCTGGTATTGCGTGTTTGGAAATACTAAAGTCCA
TGGGCGCTAAGAACATAGTGCTATGTGACAAACAAGGAGTAATACACAAAGACAGAAAAGAGGACATGAATAAGTGGAAG
GAAAAATATGCAATTGACACTAAAGAACGTTCTCTACTTGATGCCATAAAAGGCGCTGATGTATTCATCGGACTCTCTGC
AAAGGATGTGCTCAGCGAAGAGATGTTAAAAAGCATGGGTAAAGACCCGATCATTTTTGCTCTTGCTAATCCTGATCCAG
AAATAAGGCCTGAGTTTGCAAAGTCTGTAAGGCCAGATGCAATAATTGCAACTGGTAGGTCAGACTATAACAACCAAGTC
AATAACGTAATGGGGTTTCCTTATATATTCAGAGGAGCACTTGATGTACATGCAACGACAATAAATGAAGAAATGAAAAT
TGCAGCTGCAGATGCAATAGCGAAGCTTGCCCGTGAGCCAGTGCCAAATGAGATATCTGCAGCCTATGGCGGTCGTAAAA
TGAGCTATGGACGTGAATATATAATACCTACTCCATTTGATCCAAGATTAATTTCTATGGTATCTCCTGCTGTTGCAAAA
GCTGCCGTTACCTCAGGTGTGGCAAGAAAGGAAATAGAGGACTGGAATGAGTATGCAAGTCAATTAAAATCTCGCCTCGC
TAAGGCTCTCAGCACGCTCAATTTATTGTCTTCACATACGTAA

Upstream 100 bases:

>100_bases
GATAAATTTATACAGAAGTACAATAGAGTTCACAAGCTAATAGTTGAACATTCATAAAAACCCCATATACTTAGGAAATT
ACTAACAATAAGCTCAAAGC

Downstream 100 bases:

>100_bases
TTGGATAAATTTAAAAGCGATAAACCAATCCAAACTCTAAGCCATATATGCCAAACCTATTGGTAATTGTTGTAGTTGTT
GATTGCATACGTGCAGTTTC

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNNDLDNTTKQEALKYHNKSGKPGKVSVIPTKPLSTQHDLSLAYSPGVAAPCLAIAKNPKAVYDYTAKSNYVAVISNGTA
VLGLGNIGPLASKPVMEGKAVLFKRFADIDAIDIEVDTEDIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMD
IPVFHDDQHGTAVVVAAGIENALDIAEKKLENVKIIMNGSGAAGIACLEILKSMGAKNIVLCDKQGVIHKDRKEDMNKWK
EKYAIDTKERSLLDAIKGADVFIGLSAKDVLSEEMLKSMGKDPIIFALANPDPEIRPEFAKSVRPDAIIATGRSDYNNQV
NNVMGFPYIFRGALDVHATTINEEMKIAAADAIAKLAREPVPNEISAAYGGRKMSYGREYIIPTPFDPRLISMVSPAVAK
AAVTSGVARKEIEDWNEYASQLKSRLAKALSTLNLLSSHT

Sequences:

>Translated_440_residues
MNNDLDNTTKQEALKYHNKSGKPGKVSVIPTKPLSTQHDLSLAYSPGVAAPCLAIAKNPKAVYDYTAKSNYVAVISNGTA
VLGLGNIGPLASKPVMEGKAVLFKRFADIDAIDIEVDTEDIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMD
IPVFHDDQHGTAVVVAAGIENALDIAEKKLENVKIIMNGSGAAGIACLEILKSMGAKNIVLCDKQGVIHKDRKEDMNKWK
EKYAIDTKERSLLDAIKGADVFIGLSAKDVLSEEMLKSMGKDPIIFALANPDPEIRPEFAKSVRPDAIIATGRSDYNNQV
NNVMGFPYIFRGALDVHATTINEEMKIAAADAIAKLAREPVPNEISAAYGGRKMSYGREYIIPTPFDPRLISMVSPAVAK
AAVTSGVARKEIEDWNEYASQLKSRLAKALSTLNLLSSHT
>Mature_440_residues
MNNDLDNTTKQEALKYHNKSGKPGKVSVIPTKPLSTQHDLSLAYSPGVAAPCLAIAKNPKAVYDYTAKSNYVAVISNGTA
VLGLGNIGPLASKPVMEGKAVLFKRFADIDAIDIEVDTEDIEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMD
IPVFHDDQHGTAVVVAAGIENALDIAEKKLENVKIIMNGSGAAGIACLEILKSMGAKNIVLCDKQGVIHKDRKEDMNKWK
EKYAIDTKERSLLDAIKGADVFIGLSAKDVLSEEMLKSMGKDPIIFALANPDPEIRPEFAKSVRPDAIIATGRSDYNNQV
NNVMGFPYIFRGALDVHATTINEEMKIAAADAIAKLAREPVPNEISAAYGGRKMSYGREYIIPTPFDPRLISMVSPAVAK
AAVTSGVARKEIEDWNEYASQLKSRLAKALSTLNLLSSHT

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=395, Percent_Identity=25.0632911392405, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI62420882, Length=400, Percent_Identity=23.75, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI62420880, Length=400, Percent_Identity=23.75, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI239049447, Length=400, Percent_Identity=23.75, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788806, Length=427, Percent_Identity=58.07962529274, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI87081919, Length=282, Percent_Identity=24.468085106383, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=412, Percent_Identity=24.0291262135922, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=29.8892988929889, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646388, Length=401, Percent_Identity=27.6807980049875, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646386, Length=401, Percent_Identity=27.6807980049875, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707242, Length=406, Percent_Identity=25.3694581280788, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI78707238, Length=406, Percent_Identity=25.3694581280788, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI78707240, Length=342, Percent_Identity=25.4385964912281, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 47828; Mature: 47828

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDLDNTTKQEALKYHNKSGKPGKVSVIPTKPLSTQHDLSLAYSPGVAAPCLAIAKNPK
CCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHCCCCC
AVYDYTAKSNYVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAIDIEVDTED
EEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHH
IEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMDIPVFHDDQHGTAVVVAAGIE
HHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEECCCH
NALDIAEKKLENVKIIMNGSGAAGIACLEILKSMGAKNIVLCDKQGVIHKDRKEDMNKWK
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHH
EKYAIDTKERSLLDAIKGADVFIGLSAKDVLSEEMLKSMGKDPIIFALANPDPEIRPEFA
HHHCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
KSVRPDAIIATGRSDYNNQVNNVMGFPYIFRGALDVHATTINEEMKIAAADAIAKLAREP
HCCCCCEEEEECCCCCCHHHHHCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCC
VPNEISAAYGGRKMSYGREYIIPTPFDPRLISMVSPAVAKAAVTSGVARKEIEDWNEYAS
CCCHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLKSRLAKALSTLNLLSSHT
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNDLDNTTKQEALKYHNKSGKPGKVSVIPTKPLSTQHDLSLAYSPGVAAPCLAIAKNPK
CCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHCCCCC
AVYDYTAKSNYVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAIDIEVDTED
EEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHH
IEDFINAVRYLGPSWGGINLEDIRSPDCFIIEKRLNELMDIPVFHDDQHGTAVVVAAGIE
HHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEECCCH
NALDIAEKKLENVKIIMNGSGAAGIACLEILKSMGAKNIVLCDKQGVIHKDRKEDMNKWK
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHH
EKYAIDTKERSLLDAIKGADVFIGLSAKDVLSEEMLKSMGKDPIIFALANPDPEIRPEFA
HHHCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
KSVRPDAIIATGRSDYNNQVNNVMGFPYIFRGALDVHATTINEEMKIAAADAIAKLAREP
HCCCCCEEEEECCCCCCHHHHHCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCC
VPNEISAAYGGRKMSYGREYIIPTPFDPRLISMVSPAVAKAAVTSGVARKEIEDWNEYAS
CCCHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLKSRLAKALSTLNLLSSHT
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]