Click here to switch to the map view.

The map label for this gene is xth [H]

Identifier: 190571508

GI number: 190571508

Start: 1209626

End: 1210408

Strand: Reverse

Name: xth [H]

Synonym: WPa_1127

Alternate gene names: 190571508

Gene position: 1210408-1209626 (Counterclockwise)

Preceding gene: 190571509

Following gene: 190571506

Centisome position: 81.65

GC content: 33.21

Gene sequence:

>783_bases
ATGCTAAAGATTGCAACTTGGAATGTAAACTCCATACGTAAAAGAATTAACCAACTTTGTAGTTTTATAGTTGATAGTCA
GATAGATATAGTTTTGCTGCAAGAGATAAAATGTACAGAAGAGCAATTTCCTTATGCAGAGATAGAAAAGTTAGGATATG
AATATGCTGTCTATGGACAGGTTGCAAGAAATGGTGTTTGTGTTTTATCTAAATATCCAATACTGGAAAAATTAAAAATT
GATATTGTAGAGAGTCATCAAGAAGCACGTTATATAGAGTGTGTGATAAAACACTCCAATAATAAGGTAAGGGTAGGAAG
TGTATACGTTCCAAACGGTCAAAGCCCAGACTCTCATACTTTTGAGTATAAACTCAAGTTTTTTGATAACCTATATGAAA
GGATGGGCACTTTGTTGAAAAATGAAGAGTTAACTATTATAGCTGGCGATTATAATGTTGCACTGGATGAAATTGATGTT
TTTGATTCAAATTTATTAAATGGCCAAGTGTGCTTTCATATAAAAGAGCGTGAGAAATTAAGAGCGATCTTGAATCTTGG
TTTTAAAGATGCATTTAGAATATCTCACCTCAACTTACAACAATTCACTTGGTGGCATTATCAAGGTAATTCACTCAGAA
ACAATCAAGGAATGCGAATAGATCATATGCTGCTATCGCCACAAGCTGCAGATAAATTGGAAACATGTTACATAGATGAT
AGGTTGCGTAAACTAGAAAACCCATCTGATCATACTCCTGTTGTATGTGTTATAAAACAATAA

Upstream 100 bases:

>100_bases
ACATTGCTGATAGTTTAAAGTTAATCAAGTGGAAAAAAATTATAACATCGAGGTTGCCCACAGGGGAGAGTTTAATTGAT
ATAATTAATAAGGATTGTTG

Downstream 100 bases:

>100_bases
AAGACCTATTACACAATTCACCTTTGGCAAATAGGTCTTACTTGACTGCTGTCTTTTCTGTGCTTTTGGCTGGCTGATCT
ATAGTAATTTTTCCATCAAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLKIATWNVNSIRKRINQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAVYGQVARNGVCVLSKYPILEKLKI
DIVESHQEARYIECVIKHSNNKVRVGSVYVPNGQSPDSHTFEYKLKFFDNLYERMGTLLKNEELTIIAGDYNVALDEIDV
FDSNLLNGQVCFHIKEREKLRAILNLGFKDAFRISHLNLQQFTWWHYQGNSLRNNQGMRIDHMLLSPQAADKLETCYIDD
RLRKLENPSDHTPVVCVIKQ

Sequences:

>Translated_260_residues
MLKIATWNVNSIRKRINQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAVYGQVARNGVCVLSKYPILEKLKI
DIVESHQEARYIECVIKHSNNKVRVGSVYVPNGQSPDSHTFEYKLKFFDNLYERMGTLLKNEELTIIAGDYNVALDEIDV
FDSNLLNGQVCFHIKEREKLRAILNLGFKDAFRISHLNLQQFTWWHYQGNSLRNNQGMRIDHMLLSPQAADKLETCYIDD
RLRKLENPSDHTPVVCVIKQ
>Mature_260_residues
MLKIATWNVNSIRKRINQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAVYGQVARNGVCVLSKYPILEKLKI
DIVESHQEARYIECVIKHSNNKVRVGSVYVPNGQSPDSHTFEYKLKFFDNLYERMGTLLKNEELTIIAGDYNVALDEIDV
FDSNLLNGQVCFHIKEREKLRAILNLGFKDAFRISHLNLQQFTWWHYQGNSLRNNQGMRIDHMLLSPQAADKLETCYIDD
RLRKLENPSDHTPVVCVIKQ

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=31.4814814814815, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71989536, Length=265, Percent_Identity=29.0566037735849, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221330655, Length=232, Percent_Identity=30.1724137931034, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17136678, Length=232, Percent_Identity=30.1724137931034, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30261; Mature: 30261

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIATWNVNSIRKRINQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAVYGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHHHHHHCCCEEEEEEH
VARNGVCVLSKYPILEKLKIDIVESHQEARYIECVIKHSNNKVRVGSVYVPNGQSPDSHT
HHCCCEEEEECCCCHHHHEEHHHHCCCHHHEEEEEEEECCCEEEEEEEECCCCCCCCCCE
FEYKLKFFDNLYERMGTLLKNEELTIIAGDYNVALDEIDVFDSNLLNGQVCFHIKEREKL
EEEHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEHEEHHCCCCCCCEEEEEEHHHHHH
RAILNLGFKDAFRISHLNLQQFTWWHYQGNSLRNNQGMRIDHMLLSPQAADKLETCYIDD
HHHHHCCCCHHEEEEECCHHHEEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHEEEHHH
RLRKLENPSDHTPVVCVIKQ
HHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MLKIATWNVNSIRKRINQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAVYGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHHHHHHCCCEEEEEEH
VARNGVCVLSKYPILEKLKIDIVESHQEARYIECVIKHSNNKVRVGSVYVPNGQSPDSHT
HHCCCEEEEECCCCHHHHEEHHHHCCCHHHEEEEEEEECCCEEEEEEEECCCCCCCCCCE
FEYKLKFFDNLYERMGTLLKNEELTIIAGDYNVALDEIDVFDSNLLNGQVCFHIKEREKL
EEEHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEHEEHHCCCCCCCEEEEEEHHHHHH
RAILNLGFKDAFRISHLNLQQFTWWHYQGNSLRNNQGMRIDHMLLSPQAADKLETCYIDD
HHHHHCCCCHHEEEEECCHHHEEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHEEEHHH
RLRKLENPSDHTPVVCVIKQ
HHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]