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The map label for this gene is hemB [H]

Identifier: 190571482

GI number: 190571482

Start: 1170884

End: 1171882

Strand: Direct

Name: hemB [H]

Synonym: WPa_1097

Alternate gene names: 190571482

Gene position: 1170884-1171882 (Clockwise)

Preceding gene: 190571481

Following gene: 190571483

Centisome position: 78.98

GC content: 35.94

Gene sequence:

>999_bases
ATGTTTAACTTTCCAAACATAAGATTAAGACGCAGGCGCTCAGGCAAGTGGATTCGCAATTTAACAAGTGAAATCACTTT
ATCAGTAAATGATTTGATTTTTCCCTTATTTGTTCATGACGGAGAAGAAACAATTGAGCCAATTTCTGGCTTACCTGATA
TCAAATGTTATTCAATAGATGGATTAATTTCTATAGTTCAGAAAGCTAAGGACTCGGGGATTAATGCTGTTGCAATTTTT
CCTGTAGTTGATAGTAAACTAAAATCTGAAAAAGCTGAGGAAGCATATAATCCTGATAATTTAATCTGCAAAGCAATTCA
TGCTGTAAAATCGAAGGTACTTGATATTGGCATTATTGCAGACATTGCACTAGATCCATACACTACTCACGGTCATGATG
GCATTTTAAAAGACGGCAAGATGGATGTGGAAAATGATGAAACTGTGTCAATATTGTGTAAGCAAGCGCTTGTTTTAGCA
AAAGCAGGATGCGATATAGTTGCTCCTTCTGATATGATGGATGGCAGGATAGGAAAAATAAGAAAAACATTGGATGATAA
TAACTTTCAGAATGTGTCAATATTGTCCTATGCAGTAAAGTACTGTTCTAGCTTCTATGCGCCATTCAGGCAAGTTGTCG
GTTCGTGCGCATCATCAAATTTCATCGACAAAAGTGGTTATCAAATGGATTATCGAAATGCACGCGAAGCAATCTGCGAA
ATTGAAATGGATATAAATGAAGGTGCAGATTTTATTATGATAAAGCCGGGTATGCCATACTTAGATATAATAAAAACAGC
AAGTGACAAGTTCAATTTTCCGATTTTTGCTTACCAAGTAAGCGGTGAATACGCAATGATAAAAGCTGCAGCAAATAATG
GCTGGTTGGATTATAACAGAGTGATTTATGAGTCCTTAATTGGTTTTAAACGTGCAGGTGCAAGTGCAATATTTACCTAT
GCTGCACTTGATGTTGCAAAAAATTTGGTATCTACTTAG

Upstream 100 bases:

>100_bases
CTAATGCGTCTTGGTTGGAAAGTGTTTCTACCGATATCAGTGCTTTGGGTAATACTCATTTCAGGAGTGTTGCTTTTTAC
TGGGAACTTGCCTGGGTCCA

Downstream 100 bases:

>100_bases
CAGTGTGGAACATCTAAGTAGGTAGCTGGCCGAAGTGACTTGACAAACCTTCCCACTTTCCTTACCATATCAATAAGAGT
ATTTATCCTTGTTTTTAATC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MFNFPNIRLRRRRSGKWIRNLTSEITLSVNDLIFPLFVHDGEETIEPISGLPDIKCYSIDGLISIVQKAKDSGINAVAIF
PVVDSKLKSEKAEEAYNPDNLICKAIHAVKSKVLDIGIIADIALDPYTTHGHDGILKDGKMDVENDETVSILCKQALVLA
KAGCDIVAPSDMMDGRIGKIRKTLDDNNFQNVSILSYAVKYCSSFYAPFRQVVGSCASSNFIDKSGYQMDYRNAREAICE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNGWLDYNRVIYESLIGFKRAGASAIFTY
AALDVAKNLVST

Sequences:

>Translated_332_residues
MFNFPNIRLRRRRSGKWIRNLTSEITLSVNDLIFPLFVHDGEETIEPISGLPDIKCYSIDGLISIVQKAKDSGINAVAIF
PVVDSKLKSEKAEEAYNPDNLICKAIHAVKSKVLDIGIIADIALDPYTTHGHDGILKDGKMDVENDETVSILCKQALVLA
KAGCDIVAPSDMMDGRIGKIRKTLDDNNFQNVSILSYAVKYCSSFYAPFRQVVGSCASSNFIDKSGYQMDYRNAREAICE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNGWLDYNRVIYESLIGFKRAGASAIFTY
AALDVAKNLVST
>Mature_332_residues
MFNFPNIRLRRRRSGKWIRNLTSEITLSVNDLIFPLFVHDGEETIEPISGLPDIKCYSIDGLISIVQKAKDSGINAVAIF
PVVDSKLKSEKAEEAYNPDNLICKAIHAVKSKVLDIGIIADIALDPYTTHGHDGILKDGKMDVENDETVSILCKQALVLA
KAGCDIVAPSDMMDGRIGKIRKTLDDNNFQNVSILSYAVKYCSSFYAPFRQVVGSCASSNFIDKSGYQMDYRNAREAICE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNGWLDYNRVIYESLIGFKRAGASAIFTY
AALDVAKNLVST

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=302, Percent_Identity=38.4105960264901, Blast_Score=215, Evalue=4e-56,
Organism=Escherichia coli, GI87081728, Length=323, Percent_Identity=39.6284829721362, Blast_Score=224, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6321398, Length=309, Percent_Identity=37.2168284789644, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=35.7594936708861, Blast_Score=214, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36779; Mature: 36779

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNFPNIRLRRRRSGKWIRNLTSEITLSVNDLIFPLFVHDGEETIEPISGLPDIKCYSID
CCCCCCCEEEHHHCCHHHHHHHHHEEEEHHHEEEEEEEECCHHHHHHHCCCCCCEEEEHH
GLISIVQKAKDSGINAVAIFPVVDSKLKSEKAEEAYNPDNLICKAIHAVKSKVLDIGIIA
HHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
DIALDPYTTHGHDGILKDGKMDVENDETVSILCKQALVLAKAGCDIVAPSDMMDGRIGKI
EEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCHHHHH
RKTLDDNNFQNVSILSYAVKYCSSFYAPFRQVVGSCASSNFIDKSGYQMDYRNAREAICE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHEE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNGWLDYNR
EEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHH
VIYESLIGFKRAGASAIFTYAALDVAKNLVST
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFNFPNIRLRRRRSGKWIRNLTSEITLSVNDLIFPLFVHDGEETIEPISGLPDIKCYSID
CCCCCCCEEEHHHCCHHHHHHHHHEEEEHHHEEEEEEEECCHHHHHHHCCCCCCEEEEHH
GLISIVQKAKDSGINAVAIFPVVDSKLKSEKAEEAYNPDNLICKAIHAVKSKVLDIGIIA
HHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
DIALDPYTTHGHDGILKDGKMDVENDETVSILCKQALVLAKAGCDIVAPSDMMDGRIGKI
EEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCHHHHH
RKTLDDNNFQNVSILSYAVKYCSSFYAPFRQVVGSCASSNFIDKSGYQMDYRNAREAICE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHEE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNGWLDYNR
EEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHH
VIYESLIGFKRAGASAIFTYAALDVAKNLVST
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]