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The map label for this gene is trmB [H]

Identifier: 190571430

GI number: 190571430

Start: 1109920

End: 1110564

Strand: Direct

Name: trmB [H]

Synonym: WPa_1043

Alternate gene names: 190571430

Gene position: 1109920-1110564 (Clockwise)

Preceding gene: 190571426

Following gene: 190571433

Centisome position: 74.87

GC content: 30.7

Gene sequence:

>645_bases
ATGTTATCTAGGAATAATAATTGGATTAGGTCATTTTCCAGAAGATCTAGGTTGAAGTTGAATGTTTTGGAGAAGTATTC
TATTAAAAACAGCAAGGAATCTATAAAAAAGATCGTAGACTTACAAAAAAGAATACGAGTAGAAATAGGTTTCGGTAATG
GTGAAAATATGCTCCATCAGGCATTCAATGAATCTGATCTATTATTTATAGGATGTGAGCCTTATTTAAAGGGGGTCTCT
TCCTTGCTAAAAAGCATAGAAGCACACAGCATAAAAAATATTTTAATATGGACAGAAGATGCAAGAGAATTAATTGCCAA
TTTTCCTGACAACAGTGTTGAAAAATTCTTTATTCTCTTTCCAGATCCATGGCCAAAAAGAAGTCACAACAAAAGACGGT
TAATTAATACGGAATTTTTAAATTTATTAGCAAAAAAAATACTTGTCACAGGTGAAATATTTATTGCAACTGATCATCAG
GATTATGCAGAGTGGATAGAGTTACATATAAAGCAGTGTAATTTTTTAACCTATAAGGAAGATAGTTTTGCAAATTATAC
CCCAACAAAATACCACAGAAAAGCGCTAGAATATCAGCGTAAAATAAAGTTTTTTAAAGTAAATGTTATATCTAAACTGA
CTTAG

Upstream 100 bases:

>100_bases
AACTCATATATTATATAATATTAATAATTAGTGACAAGTAAATTCTCATTAATTCATTTCGAGAATTGTTTAAGCTATAA
AAGATTAAGTTATGTAAGAT

Downstream 100 bases:

>100_bases
ATTTACCTCTGCTTGTTAGTACAGATGTGTACATGACACTCTATAGTGCAAGTAGCCTACCATATACTGGGAATAACAGT
TTACTGAATTCTTTAATCTC

Product: tRNA (guanine-n(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MLSRNNNWIRSFSRRSRLKLNVLEKYSIKNSKESIKKIVDLQKRIRVEIGFGNGENMLHQAFNESDLLFIGCEPYLKGVS
SLLKSIEAHSIKNILIWTEDARELIANFPDNSVEKFFILFPDPWPKRSHNKRRLINTEFLNLLAKKILVTGEIFIATDHQ
DYAEWIELHIKQCNFLTYKEDSFANYTPTKYHRKALEYQRKIKFFKVNVISKLT

Sequences:

>Translated_214_residues
MLSRNNNWIRSFSRRSRLKLNVLEKYSIKNSKESIKKIVDLQKRIRVEIGFGNGENMLHQAFNESDLLFIGCEPYLKGVS
SLLKSIEAHSIKNILIWTEDARELIANFPDNSVEKFFILFPDPWPKRSHNKRRLINTEFLNLLAKKILVTGEIFIATDHQ
DYAEWIELHIKQCNFLTYKEDSFANYTPTKYHRKALEYQRKIKFFKVNVISKLT
>Mature_214_residues
MLSRNNNWIRSFSRRSRLKLNVLEKYSIKNSKESIKKIVDLQKRIRVEIGFGNGENMLHQAFNESDLLFIGCEPYLKGVS
SLLKSIEAHSIKNILIWTEDARELIANFPDNSVEKFFILFPDPWPKRSHNKRRLINTEFLNLLAKKILVTGEIFIATDHQ
DYAEWIELHIKQCNFLTYKEDSFANYTPTKYHRKALEYQRKIKFFKVNVISKLT

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=205, Percent_Identity=30.2439024390244, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 25342; Mature: 25342

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRNNNWIRSFSRRSRLKLNVLEKYSIKNSKESIKKIVDLQKRIRVEIGFGNGENMLHQ
CCCCCCHHHHHHHHHCCEEEEHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHH
AFNESDLLFIGCEPYLKGVSSLLKSIEAHSIKNILIWTEDARELIANFPDNSVEKFFILF
HCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCEEEEEC
PDPWPKRSHNKRRLINTEFLNLLAKKILVTGEIFIATDHQDYAEWIELHIKQCNFLTYKE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCEEEECC
DSFANYTPTKYHRKALEYQRKIKFFKVNVISKLT
CCCCCCCCHHHHHHHHHHHHHHHHEEEEEHHHCC
>Mature Secondary Structure
MLSRNNNWIRSFSRRSRLKLNVLEKYSIKNSKESIKKIVDLQKRIRVEIGFGNGENMLHQ
CCCCCCHHHHHHHHHCCEEEEHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHH
AFNESDLLFIGCEPYLKGVSSLLKSIEAHSIKNILIWTEDARELIANFPDNSVEKFFILF
HCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCEEEEEC
PDPWPKRSHNKRRLINTEFLNLLAKKILVTGEIFIATDHQDYAEWIELHIKQCNFLTYKE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCEEEECC
DSFANYTPTKYHRKALEYQRKIKFFKVNVISKLT
CCCCCCCCHHHHHHHHHHHHHHHHEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA