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The map label for this gene is ruvB

Identifier: 190571379

GI number: 190571379

Start: 1055016

End: 1056005

Strand: Reverse

Name: ruvB

Synonym: WPa_0987

Alternate gene names: 190571379

Gene position: 1056005-1055016 (Counterclockwise)

Preceding gene: 190571380

Following gene: 190571378

Centisome position: 71.23

GC content: 38.18

Gene sequence:

>990_bases
ATGAAGTCAATATCATGTAGTAAGGAATACTCTGAAGATGTACGTAATTTAAATATCCGGCCTGAGCAACTTGATGATTT
TGTCGGGCAAAAAGACTTAATACAAAATTTAAAAGTGTTTATAAATGCTGCACAGACGAGAGCTGAAGCTTTGGATCACG
TATTGCTCTATGGTCCTCCAGGGCTTGGTAAAACAACTTTAGCACAAATTGTCTCTAAGGAGTTAAGGGTTAGCTTTCGT
GCAACTTCTGGTCCATTGCTTAGTAAAGCTGGGGACTTAGCTGCAGTGCTCACTACTTTAAATGCAAAAGATGTTCTATT
TATCGATGAGATTCATAGATTAAATCGTAGCATTGAGGAAGTTTTATATACTGCTATGGAAGATTTTTGCCTAGATATAC
TAGTAGGTGAGGGCCCATCTACCAGAACTTTAAGAATAGATTTACCACCATTTACACTGATTGGGGCAACAACACGGCTT
GGACTGCTTTCTGCACCGCTGAGGGATCGTTTTGGTATTCCCCTGCATCTTGAGTTTTATTCTTTTGAGGAACTGGTTGA
TATTATAAAAAGAGGTGCAAGAGTTCTTTGTGCTGAAATCGAAAAGGATGCTGTGCAGGAAATTGCCTGCCGCGCCCGTG
GTACTCCAAGAATTGCTTTGAGATTACTCAGAAGAATAAGAGATTTTGTTGAAGTAAAAGATGATAAAAAAATTACCTGT
GAAATTGCCGGTTCTGCACTATCAAAATTGGGTATAGATAAAATGGGACTAAATAAATTAGATATGGATTATCTGAGATT
TTTGTTTAACACCTCAGGACCTGTTGGAATTGACACCATATCTATTGCACTGTCTGAAGATGTTGGCAATATTGAAGAAA
CAGTAGAACCTTATTTAATCAAGGTCAGTTTTGTAAAGCGCACACCAAGAGGACGTGTTTTAACTGATCAAGCAAGGGAA
TATCTCAGCATTAATTCAGTGGTATGCTAG

Upstream 100 bases:

>100_bases
GATATGAAAGAACAAAAGCTTATGATACAATAAAAAAAATAGAAGATGAATCACCAAACTTGGACACTAAAGATATTATT
CGTATGGCGCTTAAAACAAT

Downstream 100 bases:

>100_bases
TATTATACTATATTTTATAACATTGTATAGTATTACATTAAACATTATAATATTCTTTTATTATATGATTTGCAGGTGTT
TTAGAGGCAAATAAGAAAGT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKSISCSKEYSEDVRNLNIRPEQLDDFVGQKDLIQNLKVFINAAQTRAEALDHVLLYGPPGLGKTTLAQIVSKELRVSFR
ATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEEVLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRL
GLLSAPLRDRFGIPLHLEFYSFEELVDIIKRGARVLCAEIEKDAVQEIACRARGTPRIALRLLRRIRDFVEVKDDKKITC
EIAGSALSKLGIDKMGLNKLDMDYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLIKVSFVKRTPRGRVLTDQARE
YLSINSVVC

Sequences:

>Translated_329_residues
MKSISCSKEYSEDVRNLNIRPEQLDDFVGQKDLIQNLKVFINAAQTRAEALDHVLLYGPPGLGKTTLAQIVSKELRVSFR
ATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEEVLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRL
GLLSAPLRDRFGIPLHLEFYSFEELVDIIKRGARVLCAEIEKDAVQEIACRARGTPRIALRLLRRIRDFVEVKDDKKITC
EIAGSALSKLGIDKMGLNKLDMDYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLIKVSFVKRTPRGRVLTDQARE
YLSINSVVC
>Mature_329_residues
MKSISCSKEYSEDVRNLNIRPEQLDDFVGQKDLIQNLKVFINAAQTRAEALDHVLLYGPPGLGKTTLAQIVSKELRVSFR
ATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEEVLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRL
GLLSAPLRDRFGIPLHLEFYSFEELVDIIKRGARVLCAEIEKDAVQEIACRARGTPRIALRLLRRIRDFVEVKDDKKITC
EIAGSALSKLGIDKMGLNKLDMDYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLIKVSFVKRTPRGRVLTDQARE
YLSINSVVC

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=324, Percent_Identity=53.3950617283951, Blast_Score=350, Evalue=8e-98,
Organism=Escherichia coli, GI1787119, Length=316, Percent_Identity=25, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_WOLPP (B3CMH5)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975737.1
- EnsemblBacteria:   EBWOLT00000001005
- GeneID:   6385078
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0987
- GeneTree:   EBGT00050000031430
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 36667; Mature: 36667

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSISCSKEYSEDVRNLNIRPEQLDDFVGQKDLIQNLKVFINAAQTRAEALDHVLLYGPP
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
GLGKTTLAQIVSKELRVSFRATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEE
CCCHHHHHHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
VLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRLGLLSAPLRDRFGIPLHLEFY
HHHHHHHHHHHHHEECCCCCCEEEEEECCCEEEHHHHHHHHHHCCCHHHHCCCCEEEECC
SFEELVDIIKRGARVLCAEIEKDAVQEIACRARGTPRIALRLLRRIRDFVEVKDDKKITC
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
EIAGSALSKLGIDKMGLNKLDMDYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLI
EEHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHCCCEEE
KVSFVKRTPRGRVLTDQAREYLSINSVVC
EEEEEEECCCCCEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSISCSKEYSEDVRNLNIRPEQLDDFVGQKDLIQNLKVFINAAQTRAEALDHVLLYGPP
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
GLGKTTLAQIVSKELRVSFRATSGPLLSKAGDLAAVLTTLNAKDVLFIDEIHRLNRSIEE
CCCHHHHHHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
VLYTAMEDFCLDILVGEGPSTRTLRIDLPPFTLIGATTRLGLLSAPLRDRFGIPLHLEFY
HHHHHHHHHHHHHEECCCCCCEEEEEECCCEEEHHHHHHHHHHCCCHHHHCCCCEEEECC
SFEELVDIIKRGARVLCAEIEKDAVQEIACRARGTPRIALRLLRRIRDFVEVKDDKKITC
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
EIAGSALSKLGIDKMGLNKLDMDYLRFLFNTSGPVGIDTISIALSEDVGNIEETVEPYLI
EEHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHCCCEEE
KVSFVKRTPRGRVLTDQAREYLSINSVVC
EEEEEEECCCCCEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA