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The map label for this gene is rpoH [H]

Identifier: 190571331

GI number: 190571331

Start: 1004753

End: 1005646

Strand: Reverse

Name: rpoH [H]

Synonym: WPa_0936

Alternate gene names: 190571331

Gene position: 1005646-1004753 (Counterclockwise)

Preceding gene: 190571332

Following gene: 190571330

Centisome position: 67.84

GC content: 33.45

Gene sequence:

>894_bases
ATGTTGACCCCAGTAGCAAATTTATGTGTTAATAGTAGCACTAATCTCATGCAATATATTGCTGAAGTGCGAAAATTTCC
TATGCTTTCCCAAGAAGAGGAAGTGCGATTAGCAAAAAATTGGCAAGAATACCAGGATATTTCTTCTGCTCACAGGTTGA
TTACTAGCCATTTGAATTTGGTAGTAAAAATTGCAATGAAATTTAAGAATTACGGTCTATTGTTGATGGACCTGGTCATG
GAAGGAAATATGGGTTTAATGCATGCAGTGAAAAAGTTCAATCCTGACTTGGGATTTCGTTTTTCAACTTATGCAGTATG
GTGGATTGAAGCTTCAATGAAAGACTTTATATTAAAATCTTGGTCACTTGTAAAAATAGGTACAACGCAAGCACAAAGGA
GATTGTTTTTTAGTTTACGTAAAATAAAAAAAAGAATTTTACAATACACAAACAGAGAAACTTTGAGTAAAGAAGAAATA
AAAGCAATATCAAATGAGCTTTCTGTATCTGAAGAAGATGTTGTACAGATGAGCTATCGTCTGGCTTGTAAGGATCAGTC
GTTAAATAATTGTATAAAAATAGGTGATGACTCTAAAAAGGAGTTACAAGATATGATTCCGTGTAATTTAGTTAGTCAAG
AAGTAGCTTACCAGAATCATGAAGAAAAGGCATTAAAAGAAACAATTTTAAGCAATGCGCTGGCAAGTCTTAATGAAAGA
TCAAGAGACATTTTCATTAGCAGATATTTATCTGAGAATACTCAGACTTTGGATGAGCTTAGCAAAAAATATTGTGTTTC
AAGAGAAAGAATAAGACAGCTGGCTGAGCAAGCGATGAGTAAGGTAAAACAACATATAAAATCAGAGAGTTTGAAATTCG
GTTTACATGCGTAG

Upstream 100 bases:

>100_bases
AGATCTCAACTAGATATAAAGTTATGCTTTTTTAATTAAAGCTTGAATTTTTCACTGAAAATCAATATATTATATGTATA
GAGTTTTAATTTAAGTTATT

Downstream 100 bases:

>100_bases
TCTTTTCGTATTTCTAATATTTCTTTCAATAGACACTCTTGCATCGGTTAGTAATGTACAACTGAGCGAAAAATATAAAG
ACTGGTTTGTGTATACTGCA

Product: heat shock sigma factor RpoH

Products: diphosphate; RNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLTPVANLCVNSSTNLMQYIAEVRKFPMLSQEEEVRLAKNWQEYQDISSAHRLITSHLNLVVKIAMKFKNYGLLLMDLVM
EGNMGLMHAVKKFNPDLGFRFSTYAVWWIEASMKDFILKSWSLVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLSKEEI
KAISNELSVSEEDVVQMSYRLACKDQSLNNCIKIGDDSKKELQDMIPCNLVSQEVAYQNHEEKALKETILSNALASLNER
SRDIFISRYLSENTQTLDELSKKYCVSRERIRQLAEQAMSKVKQHIKSESLKFGLHA

Sequences:

>Translated_297_residues
MLTPVANLCVNSSTNLMQYIAEVRKFPMLSQEEEVRLAKNWQEYQDISSAHRLITSHLNLVVKIAMKFKNYGLLLMDLVM
EGNMGLMHAVKKFNPDLGFRFSTYAVWWIEASMKDFILKSWSLVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLSKEEI
KAISNELSVSEEDVVQMSYRLACKDQSLNNCIKIGDDSKKELQDMIPCNLVSQEVAYQNHEEKALKETILSNALASLNER
SRDIFISRYLSENTQTLDELSKKYCVSRERIRQLAEQAMSKVKQHIKSESLKFGLHA
>Mature_297_residues
MLTPVANLCVNSSTNLMQYIAEVRKFPMLSQEEEVRLAKNWQEYQDISSAHRLITSHLNLVVKIAMKFKNYGLLLMDLVM
EGNMGLMHAVKKFNPDLGFRFSTYAVWWIEASMKDFILKSWSLVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLSKEEI
KAISNELSVSEEDVVQMSYRLACKDQSLNNCIKIGDDSKKELQDMIPCNLVSQEVAYQNHEEKALKETILSNALASLNER
SRDIFISRYLSENTQTLDELSKKYCVSRERIRQLAEQAMSKVKQHIKSESLKFGLHA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=296, Percent_Identity=37.5, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789098, Length=293, Percent_Identity=31.0580204778157, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=31.8181818181818, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: 2.7.7.6

Molecular weight: Translated: 34431; Mature: 34431

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPVANLCVNSSTNLMQYIAEVRKFPMLSQEEEVRLAKNWQEYQDISSAHRLITSHLNL
CCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVKIAMKFKNYGLLLMDLVMEGNMGLMHAVKKFNPDLGFRFSTYAVWWIEASMKDFILKS
HHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHEEEEEEEHHHHHHHHHC
WSLVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLSKEEIKAISNELSVSEEDVVQMSYR
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCHHHHHHHHHH
LACKDQSLNNCIKIGDDSKKELQDMIPCNLVSQEVAYQNHEEKALKETILSNALASLNER
HHHCCCCHHHHEECCCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
SRDIFISRYLSENTQTLDELSKKYCVSRERIRQLAEQAMSKVKQHIKSESLKFGLHA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTPVANLCVNSSTNLMQYIAEVRKFPMLSQEEEVRLAKNWQEYQDISSAHRLITSHLNL
CCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVKIAMKFKNYGLLLMDLVMEGNMGLMHAVKKFNPDLGFRFSTYAVWWIEASMKDFILKS
HHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHEEEEEEEHHHHHHHHHC
WSLVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLSKEEIKAISNELSVSEEDVVQMSYR
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCHHHHHHHHHH
LACKDQSLNNCIKIGDDSKKELQDMIPCNLVSQEVAYQNHEEKALKETILSNALASLNER
HHHCCCCHHHHEECCCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
SRDIFISRYLSENTQTLDELSKKYCVSRERIRQLAEQAMSKVKQHIKSESLKFGLHA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]