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The map label for this gene is dnaN [H]

Identifier: 190571329

GI number: 190571329

Start: 1003000

End: 1004151

Strand: Reverse

Name: dnaN [H]

Synonym: WPa_0934

Alternate gene names: 190571329

Gene position: 1004151-1003000 (Counterclockwise)

Preceding gene: 190571330

Following gene: 190571327

Centisome position: 67.74

GC content: 34.11

Gene sequence:

>1152_bases
ATGTCAAAAATTGTAGATATAGGAATCAAAGAACAAAGTTCTGTGTGTGAACAAATGCGTTTTAGTGTAAGTCGTGCAAG
CCTTTTAAATACGCTATCCAGGGTAAGTGGAGTGGTTGAAAGGCGCAACGCGATAGATGTTTTAGCGTGTATAAATATCA
AAGCACAATATGGTAGCATAAAATTGAAGGCTACTGATCTTGACATTTCAATATTTGCCTCACTTACTGCAAATGTATCA
GCAGAAGGAGAAGTGAAAATCTCAGCACATACTTTACATGATATAGTGAAAAAATTGCCAACTAATTTGGATGTCAATTT
CGAAATGAATAATCAAGGAAAATTATTGATATCCTGTGGAAATGCAAATTTTTCTTTACCGAACGTAGTTTCAAATAACT
TCCCTGTTCTTGAAGAAGGTGATCATCAATACGATTTCACTCTACTAAGTGCAGATTTGGTAGACTTGTTGACTAAAACG
AAGTTTGCTGTGTCGCTAGATGATACAAGGTATAATTTGAATGGGATATATCTGCATACAGATGAGCAATTTCTGTACTG
CGTTGCAACTGATGGCCACCGTTTATCATGTATAAAAAGGCCTAAACCTGAAGATATCAACGGAGAATTTGGGGTGATCA
TTCCACGTAAAACTATCATGGAACTGTTGAAAGTATTGGATGATTGTAATGAGATAAATATAAAACTTTCAGATAGAAAA
ATCAAATTTACATGTGGTGAGTATATTCTAATATCAAAATTGATAGATGGAACTTTTCCAGATTACAAAGCCGTTATTCC
AACGTCTCAAGATAAACAAATGATTGTTGAGAGTAGCAAACTTGCTAGTGTTATAGACCGAGTTTCCGTTGTTGTGTCCG
ATAAAATAAAATCAATTAAATTTTCATTGCAAGAGAATTTATTAACTCTACATTCAAATTCCCAAGAGTGTAGTGATGCA
ACTGAGTCTATAGAAGTGGATTACGATGGAGCTCTTATGGAAGTAGGATTTAATTCTCGTTATTTGCTTGATGTTTTATC
CTGTATAGAAAACAAGTGTAAATTTAGCTTATCAGATGGTAATAGCGCTACAATTATCACTGATGAAGATGATCCAAGTG
CACTATATATAGTAATGCCAATGAGGACTTAA

Upstream 100 bases:

>100_bases
CTAAAGTATAAGATAGGCTGTTTTGTCTCTCAGACAAAAATATGGATATCACTTTACATAGAATATGAATATCATTTAAA
ATATATTTTTGAGAATTAAT

Downstream 100 bases:

>100_bases
GCTAGTAATCTATCTTTACTAAGTATAAGCCGTGAGGCGGTGCAGTGATTCCAGCAGCGTTTCTTTTACATTGACTTAAT
ATGTTTAGCATTTCTTGTGG

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSKIVDIGIKEQSSVCEQMRFSVSRASLLNTLSRVSGVVERRNAIDVLACINIKAQYGSIKLKATDLDISIFASLTANVS
AEGEVKISAHTLHDIVKKLPTNLDVNFEMNNQGKLLISCGNANFSLPNVVSNNFPVLEEGDHQYDFTLLSADLVDLLTKT
KFAVSLDDTRYNLNGIYLHTDEQFLYCVATDGHRLSCIKRPKPEDINGEFGVIIPRKTIMELLKVLDDCNEINIKLSDRK
IKFTCGEYILISKLIDGTFPDYKAVIPTSQDKQMIVESSKLASVIDRVSVVVSDKIKSIKFSLQENLLTLHSNSQECSDA
TESIEVDYDGALMEVGFNSRYLLDVLSCIENKCKFSLSDGNSATIITDEDDPSALYIVMPMRT

Sequences:

>Translated_383_residues
MSKIVDIGIKEQSSVCEQMRFSVSRASLLNTLSRVSGVVERRNAIDVLACINIKAQYGSIKLKATDLDISIFASLTANVS
AEGEVKISAHTLHDIVKKLPTNLDVNFEMNNQGKLLISCGNANFSLPNVVSNNFPVLEEGDHQYDFTLLSADLVDLLTKT
KFAVSLDDTRYNLNGIYLHTDEQFLYCVATDGHRLSCIKRPKPEDINGEFGVIIPRKTIMELLKVLDDCNEINIKLSDRK
IKFTCGEYILISKLIDGTFPDYKAVIPTSQDKQMIVESSKLASVIDRVSVVVSDKIKSIKFSLQENLLTLHSNSQECSDA
TESIEVDYDGALMEVGFNSRYLLDVLSCIENKCKFSLSDGNSATIITDEDDPSALYIVMPMRT
>Mature_382_residues
SKIVDIGIKEQSSVCEQMRFSVSRASLLNTLSRVSGVVERRNAIDVLACINIKAQYGSIKLKATDLDISIFASLTANVSA
EGEVKISAHTLHDIVKKLPTNLDVNFEMNNQGKLLISCGNANFSLPNVVSNNFPVLEEGDHQYDFTLLSADLVDLLTKTK
FAVSLDDTRYNLNGIYLHTDEQFLYCVATDGHRLSCIKRPKPEDINGEFGVIIPRKTIMELLKVLDDCNEINIKLSDRKI
KFTCGEYILISKLIDGTFPDYKAVIPTSQDKQMIVESSKLASVIDRVSVVVSDKIKSIKFSLQENLLTLHSNSQECSDAT
ESIEVDYDGALMEVGFNSRYLLDVLSCIENKCKFSLSDGNSATIITDEDDPSALYIVMPMRT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=369, Percent_Identity=33.0623306233062, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42533; Mature: 42402

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVDIGIKEQSSVCEQMRFSVSRASLLNTLSRVSGVVERRNAIDVLACINIKAQYGSI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCEE
KLKATDLDISIFASLTANVSAEGEVKISAHTLHDIVKKLPTNLDVNFEMNNQGKLLISCG
EEEEECCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEC
NANFSLPNVVSNNFPVLEEGDHQYDFTLLSADLVDLLTKTKFAVSLDDTRYNLNGIYLHT
CCCCCCCCHHCCCCCCEECCCCEEEEEEEHHHHHHHHHCCEEEEEECCCEEEEEEEEEEE
DEQFLYCVATDGHRLSCIKRPKPEDINGEFGVIIPRKTIMELLKVLDDCNEINIKLSDRK
CCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEEECCE
IKFTCGEYILISKLIDGTFPDYKAVIPTSQDKQMIVESSKLASVIDRVSVVVSDKIKSIK
EEEECCCEEEEEHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLQENLLTLHSNSQECSDATESIEVDYDGALMEVGFNSRYLLDVLSCIENKCKFSLSDG
HHHHHCEEEEECCCHHHCCHHHEEEEECCCEEEEECCCCHHHHHHHHHHHHHCEEEECCC
NSATIITDEDDPSALYIVMPMRT
CEEEEEECCCCCCEEEEEEECCC
>Mature Secondary Structure 
SKIVDIGIKEQSSVCEQMRFSVSRASLLNTLSRVSGVVERRNAIDVLACINIKAQYGSI
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCEE
KLKATDLDISIFASLTANVSAEGEVKISAHTLHDIVKKLPTNLDVNFEMNNQGKLLISCG
EEEEECCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEC
NANFSLPNVVSNNFPVLEEGDHQYDFTLLSADLVDLLTKTKFAVSLDDTRYNLNGIYLHT
CCCCCCCCHHCCCCCCEECCCCEEEEEEEHHHHHHHHHCCEEEEEECCCEEEEEEEEEEE
DEQFLYCVATDGHRLSCIKRPKPEDINGEFGVIIPRKTIMELLKVLDDCNEINIKLSDRK
CCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEEECCE
IKFTCGEYILISKLIDGTFPDYKAVIPTSQDKQMIVESSKLASVIDRVSVVVSDKIKSIK
EEEECCCEEEEEHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLQENLLTLHSNSQECSDATESIEVDYDGALMEVGFNSRYLLDVLSCIENKCKFSLSDG
HHHHHCEEEEECCCHHHCCHHHEEEEECCCEEEEECCCCHHHHHHHHHHHHHCEEEECCC
NSATIITDEDDPSALYIVMPMRT
CEEEEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]