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The map label for this gene is folD

Identifier: 190571146

GI number: 190571146

Start: 811672

End: 812559

Strand: Reverse

Name: folD

Synonym: WPa_0744

Alternate gene names: 190571146

Gene position: 812559-811672 (Counterclockwise)

Preceding gene: 190571147

Following gene: 190571129

Centisome position: 54.81

GC content: 34.68

Gene sequence:

>888_bases
ATGTCAATTATTATTAACGGTAAAAAAACAGCAAATGATCTCTGTGAGGAGCTATCAAAAAAAATTGATATTTTAAAGAA
AGAGTATAATGTTTTTCCTTGTCTTAAAGTAATTCTCGTGGGTAGCAATCCAGCAAGTCAGGTCTACGTTCGTAATAAAC
AAAGAAAAGCAGAATCAATAGGCATAAGTTCTGAAACTATTGTTTTGCCTGATAGTATCTCAGAAGATGAATTGATTGAA
AAAGTTAATACATTAAATGAAGATTCATCTGTGCATGGAATTTTAGTTCAGTTACCTTTACCAAAGCACATCAGTGCAAG
CAGAATAATTAATACAGTAAGTGTTGAAAAAGATGTAGATGGCTTTCATGATGAGAATGTTGGCAGGTTAGTTAAAGGTG
AAAAAAATTGTCTGATACCTTGTACTCCCAAAGGTTCTTTGCATTTAATTAAGTCAGTTGAAAATAATCTGTCCGGTAAA
AATGCGGTAATCATTGGCAGATCAAATATTGTGGGTAAGCCAATGTTTCACCTATTGTTGCAAGAAAATTGCACTGTTAC
CATCTTGCATTCACAAAGTAGGGATTTAGCTGATTATTGCTCTAAGGCGGATATAGTAGTTGCAGCAGTTGGAAAGCCAA
ATTTTGTTCAACCAGATTGGATAAAAAAAGGTGCAATAGTGATTGATGTTGGCATAAATAGCATTAACATAGGAAATCAA
AGTAAGCTTGTGGGTGATGTTGACTTTGAGGGAGTAAAAGAAAAAGTCAAAGCTATTACTCCAGTGCCTGGTGGAGTTGG
CCCGATGACAATAGCATTTCTTATGATCAACACTTTAGTTGCTGCTTGCTTACAGAAGGGAGTTGATCCTTCTGATTTTA
TTGCTTAA

Upstream 100 bases:

>100_bases
ATTAAACCAAAATCTTTGGATGATATTGTAAATCATTCTGTTGGTAAAGTATTGAATTTATTTGATATCGCATTACCTGA
CTTCGAGGAGTGGCAAGGAA

Downstream 100 bases:

>100_bases
GGTTTAGCTTAATGTTAGCATAAACAGAATACCTTTGCAAATAGCACATATATAGCACCAAGCATACAACTGTGTATAGG
GATTCAAAGTGTTCATAGCT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSIIINGKKTANDLCEELSKKIDILKKEYNVFPCLKVILVGSNPASQVYVRNKQRKAESIGISSETIVLPDSISEDELIE
KVNTLNEDSSVHGILVQLPLPKHISASRIINTVSVEKDVDGFHDENVGRLVKGEKNCLIPCTPKGSLHLIKSVENNLSGK
NAVIIGRSNIVGKPMFHLLLQENCTVTILHSQSRDLADYCSKADIVVAAVGKPNFVQPDWIKKGAIVIDVGINSINIGNQ
SKLVGDVDFEGVKEKVKAITPVPGGVGPMTIAFLMINTLVAACLQKGVDPSDFIA

Sequences:

>Translated_295_residues
MSIIINGKKTANDLCEELSKKIDILKKEYNVFPCLKVILVGSNPASQVYVRNKQRKAESIGISSETIVLPDSISEDELIE
KVNTLNEDSSVHGILVQLPLPKHISASRIINTVSVEKDVDGFHDENVGRLVKGEKNCLIPCTPKGSLHLIKSVENNLSGK
NAVIIGRSNIVGKPMFHLLLQENCTVTILHSQSRDLADYCSKADIVVAAVGKPNFVQPDWIKKGAIVIDVGINSINIGNQ
SKLVGDVDFEGVKEKVKAITPVPGGVGPMTIAFLMINTLVAACLQKGVDPSDFIA
>Mature_294_residues
SIIINGKKTANDLCEELSKKIDILKKEYNVFPCLKVILVGSNPASQVYVRNKQRKAESIGISSETIVLPDSISEDELIEK
VNTLNEDSSVHGILVQLPLPKHISASRIINTVSVEKDVDGFHDENVGRLVKGEKNCLIPCTPKGSLHLIKSVENNLSGKN
AVIIGRSNIVGKPMFHLLLQENCTVTILHSQSRDLADYCSKADIVVAAVGKPNFVQPDWIKKGAIVIDVGINSINIGNQS
KLVGDVDFEGVKEKVKAITPVPGGVGPMTIAFLMINTLVAACLQKGVDPSDFIA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=286, Percent_Identity=45.8041958041958, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=48.4641638225256, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=45.3924914675768, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI36796743, Length=211, Percent_Identity=29.3838862559242, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1786741, Length=289, Percent_Identity=47.4048442906574, Blast_Score=254, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI17568735, Length=286, Percent_Identity=41.958041958042, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=49.4773519163763, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=44.4067796610169, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=315, Percent_Identity=28.2539682539683, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=45.4545454545455, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=45.4545454545455, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=45.4545454545455, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=45.4545454545455, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=45.9731543624161, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=45.9731543624161, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_WOLPP (B3CLT4)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975504.1
- EnsemblBacteria:   EBWOLT00000000287
- GeneID:   6384961
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0744
- GeneTree:   EBGT00050000031040
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- ProtClustDB:   PRK14187
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31868; Mature: 31737

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIINGKKTANDLCEELSKKIDILKKEYNVFPCLKVILVGSNPASQVYVRNKQRKAESI
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECHHHHHHHC
GISSETIVLPDSISEDELIEKVNTLNEDSSVHGILVQLPLPKHISASRIINTVSVEKDVD
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHEECCCCCC
GFHDENVGRLVKGEKNCLIPCTPKGSLHLIKSVENNLSGKNAVIIGRSNIVGKPMFHLLL
CCCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
QENCTVTILHSQSRDLADYCSKADIVVAAVGKPNFVQPDWIKKGAIVIDVGINSINIGNQ
HCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCCCCCCCHHCCCCEEEEECCCEEECCCC
SKLVGDVDFEGVKEKVKAITPVPGGVGPMTIAFLMINTLVAACLQKGVDPSDFIA
CCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SIIINGKKTANDLCEELSKKIDILKKEYNVFPCLKVILVGSNPASQVYVRNKQRKAESI
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECHHHHHHHC
GISSETIVLPDSISEDELIEKVNTLNEDSSVHGILVQLPLPKHISASRIINTVSVEKDVD
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHEECCCCCC
GFHDENVGRLVKGEKNCLIPCTPKGSLHLIKSVENNLSGKNAVIIGRSNIVGKPMFHLLL
CCCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
QENCTVTILHSQSRDLADYCSKADIVVAAVGKPNFVQPDWIKKGAIVIDVGINSINIGNQ
HCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCCCCCCCHHCCCCEEEEECCCEEECCCC
SKLVGDVDFEGVKEKVKAITPVPGGVGPMTIAFLMINTLVAACLQKGVDPSDFIA
CCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA