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The map label for this gene is fmt

Identifier: 190571066

GI number: 190571066

Start: 727759

End: 728658

Strand: Reverse

Name: fmt

Synonym: WPa_0661

Alternate gene names: 190571066

Gene position: 728658-727759 (Counterclockwise)

Preceding gene: 190571067

Following gene: 190571064

Centisome position: 49.15

GC content: 35.44

Gene sequence:

>900_bases
ATGAGAATTATTTTCATGGGGTCACCGGGGTTTGCTGTTGGTGCGTTAAACTTACTATTGAAATTACAGAGTGAAGTAGT
AGCAGTATATACCAAAGCTCCAAAGCCTTCTGGACGTAGGCAGAGGTTAACAAAATCGCCAGTACACATCGTTGCTGAAA
AAAGTGACATAGAGGTATGTACTCCTGCCTCTTTAAAATCTTCGATAGAGCAAGAAAAGTTTGGAAATTTTAAGCCAGAC
GTTGCAGTTGTTGCTGCGTATGGATTGATACTTCCAAAAGAAATTTTAAATATTCCCAAATATGGTTGTATTAATATTCA
TCCATCATTGCTGCCTAGGTGGCGCGGTGCAGCTCCGATACAGCACACAATTTTAGCCGGAGATCAAGAAACCGGAGTTA
GCATTATGCAGCTAGATGAAGGATTAGATTCTGGCCCTATTTTAAAACAGAAAAAATTTCTTATTGAAAAAAGCGATAAT
TATAAGACGTTGTATGATAAATTGTCTGAATTAGGTAGTGATTTACTGCTGAAAGTACTAAACGAAATTGAAAAGCAAGT
TCCCTTAAAACAGAGCGATAACGATGCGTGCTATGCTGACAAAGTAAAAGACTATAAAATTTACGCAAGTGACGCCTGTG
AAATTGCTTATAGAAAGGTTAAAGCATTTTATCCAAAAGCATTCATTAAGGTAGAGAATAAACGTATCAAGATACTTGAT
GCTGAATTTGAAGCCTTCACTTCAGGACAAGGTGAGATCATTAATGATAATATGCATATAAGTTTAAAAGGTGGTACTTT
AATTCCTAAAGTTGTACAAATGGAAGGAAGAAATCCTTGCGATATTAAAGATTTTGTTCGTGGCTTAAAATCAAGTTTAA
CAAAAAAATTTATAGAATAG

Upstream 100 bases:

>100_bases
GCTGAGATGGCTGTTAATCATAAGGATGATTTTGCTAAATTAATAGAAACTGTAAGTAGTAAGTTAGCAGAAAATTCCTA
AAGCAAACACACCTGAAAAC

Downstream 100 bases:

>100_bases
ACTACTTTGAAAGGAGAGTGAGGAGAGCTAAAGCAACTTCTACAATTTGGTATTCCGCTGCTTGTTAGCGGAATCTACAA
CAAGATACCGCGTTTTGGTG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRIIFMGSPGFAVGALNLLLKLQSEVVAVYTKAPKPSGRRQRLTKSPVHIVAEKSDIEVCTPASLKSSIEQEKFGNFKPD
VAVVAAYGLILPKEILNIPKYGCINIHPSLLPRWRGAAPIQHTILAGDQETGVSIMQLDEGLDSGPILKQKKFLIEKSDN
YKTLYDKLSELGSDLLLKVLNEIEKQVPLKQSDNDACYADKVKDYKIYASDACEIAYRKVKAFYPKAFIKVENKRIKILD
AEFEAFTSGQGEIINDNMHISLKGGTLIPKVVQMEGRNPCDIKDFVRGLKSSLTKKFIE

Sequences:

>Translated_299_residues
MRIIFMGSPGFAVGALNLLLKLQSEVVAVYTKAPKPSGRRQRLTKSPVHIVAEKSDIEVCTPASLKSSIEQEKFGNFKPD
VAVVAAYGLILPKEILNIPKYGCINIHPSLLPRWRGAAPIQHTILAGDQETGVSIMQLDEGLDSGPILKQKKFLIEKSDN
YKTLYDKLSELGSDLLLKVLNEIEKQVPLKQSDNDACYADKVKDYKIYASDACEIAYRKVKAFYPKAFIKVENKRIKILD
AEFEAFTSGQGEIINDNMHISLKGGTLIPKVVQMEGRNPCDIKDFVRGLKSSLTKKFIE
>Mature_299_residues
MRIIFMGSPGFAVGALNLLLKLQSEVVAVYTKAPKPSGRRQRLTKSPVHIVAEKSDIEVCTPASLKSSIEQEKFGNFKPD
VAVVAAYGLILPKEILNIPKYGCINIHPSLLPRWRGAAPIQHTILAGDQETGVSIMQLDEGLDSGPILKQKKFLIEKSDN
YKTLYDKLSELGSDLLLKVLNEIEKQVPLKQSDNDACYADKVKDYKIYASDACEIAYRKVKAFYPKAFIKVENKRIKILD
AEFEAFTSGQGEIINDNMHISLKGGTLIPKVVQMEGRNPCDIKDFVRGLKSSLTKKFIE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=258, Percent_Identity=32.9457364341085, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI21614513, Length=173, Percent_Identity=31.7919075144509, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI238814322, Length=173, Percent_Identity=30.0578034682081, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789683, Length=297, Percent_Identity=38.047138047138, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788589, Length=255, Percent_Identity=25.0980392156863, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI133930964, Length=179, Percent_Identity=34.0782122905028, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=227, Percent_Identity=27.3127753303965, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550868, Length=231, Percent_Identity=32.9004329004329, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571984, Length=153, Percent_Identity=38.562091503268, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585660, Length=170, Percent_Identity=32.9411764705882, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_WOLPP (B3CLK1)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975424.1
- EnsemblBacteria:   EBWOLT00000000408
- GeneID:   6385267
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0661
- GeneTree:   EBGT00050000031330
- HOGENOM:   HBG571560
- OMA:   WRGAGPI
- ProtClustDB:   CLSK749560
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33285; Mature: 33285

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFMGSPGFAVGALNLLLKLQSEVVAVYTKAPKPSGRRQRLTKSPVHIVAEKSDIEVC
CEEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHCCCCCEEEEEECCCCCEE
TPASLKSSIEQEKFGNFKPDVAVVAAYGLILPKEILNIPKYGCINIHPSLLPRWRGAAPI
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCCCC
QHTILAGDQETGVSIMQLDEGLDSGPILKQKKFLIEKSDNYKTLYDKLSELGSDLLLKVL
HHEEEECCCCCCCEEEEECCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHH
NEIEKQVPLKQSDNDACYADKVKDYKIYASDACEIAYRKVKAFYPKAFIKVENKRIKILD
HHHHHHCCCCCCCCCCEECCCCCCEEEEECHHHHHHHHHHHHHCCEEEEEECCCEEEEEE
AEFEAFTSGQGEIINDNMHISLKGGTLIPKVVQMEGRNPCDIKDFVRGLKSSLTKKFIE
CCHHHHCCCCCEEECCCEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIIFMGSPGFAVGALNLLLKLQSEVVAVYTKAPKPSGRRQRLTKSPVHIVAEKSDIEVC
CEEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHCCCCCEEEEEECCCCCEE
TPASLKSSIEQEKFGNFKPDVAVVAAYGLILPKEILNIPKYGCINIHPSLLPRWRGAAPI
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCCCC
QHTILAGDQETGVSIMQLDEGLDSGPILKQKKFLIEKSDNYKTLYDKLSELGSDLLLKVL
HHEEEECCCCCCCEEEEECCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHH
NEIEKQVPLKQSDNDACYADKVKDYKIYASDACEIAYRKVKAFYPKAFIKVENKRIKILD
HHHHHHCCCCCCCCCCEECCCCCCEEEEECHHHHHHHHHHHHHCCEEEEEECCCEEEEEE
AEFEAFTSGQGEIINDNMHISLKGGTLIPKVVQMEGRNPCDIKDFVRGLKSSLTKKFIE
CCHHHHCCCCCEEECCCEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA