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The map label for this gene is pheT [H]

Identifier: 190571033

GI number: 190571033

Start: 676579

End: 678918

Strand: Direct

Name: pheT [H]

Synonym: WPa_0625

Alternate gene names: 190571033

Gene position: 676579-678918 (Clockwise)

Preceding gene: 190571032

Following gene: 190571035

Centisome position: 45.64

GC content: 34.79

Gene sequence:

>2340_bases
ATGAAGTTCACATTATCTTGGTTATTAGAGTACTTAGAAACCAATGCTAGTTTAGAGGAAATTACTGATAAATTAACTCA
CATAGGGTTGGAAGTAGAGAATGTGATCGACAATTCCAGATTGGCCGGGTTTGTTGTTGCAGAAGTATTAGAAGTCGCAC
CTCATCCAAATGCTGATAAATTAAAATTATGTAGAGTAAATGATGGAAGTAAAGTTCTGCAGATAGTCTGCGGAGCAAAA
AATGTTAAAGAAGGCATGAAGACTGTACTTGCATCTCTTGGTAGCACATTGCCAGAAAGCAACTTTACAATTAAGCCTAC
AAAAATAAGAGGAGTGCTAAGTGAAGGGATGCTCTGCTCTGCCTCTGAGCTTTCGCTGACTCAAGATGAAAGTGAAGGAA
TAATCGAGCTTTCTGATGATTATAAAACAGGAGATAAATTTTTCAATTGCGATCCTGTAATTGACATAAACATCACTCCA
AACCGTGGAGATTGCTTAGGTGTTTATGGAATAGCACGTGATCTAGCAGCAACTGGAATTGGAACGTTAAAGACTTTAAT
CATTCCACAACTTACCGCTTCTATGAACTCACTAATAAACGTCAAAGTCATCGATGGGGAGAGTTTTATTAGTGGAGTAT
ATATCACTAATGTAAAAAATAAAGAGAGTCCAAAATGGCTAAAAGATAAATTGGAATCGATAGGAATGCGCTCCATTTCT
GCAATAGTTGATATTACTAATTACATTATGATATCTTTCGGGCGCCCGATGCACGCGTATGATGCAAAAAAAATAGAAGG
AGAGCTCATAGTACGCAAAGCAAATGATGAAGAAAAATTTATTGGTTTAAATGATAAAGAGTACTTATTAAACAAAGACA
TAAGCGTTATTTCTGATGATAAAAATGTTCATGGAATTGCTGGAATTATGGGCAGCAAGTATAGCGAATGTACACTTAAA
ACTACTGATATCTTTTTAGAGTCTGCTTGGTTTGACCCCATCTCCATCACTAAATCTTCAAGGCAGCTAGGCATCTCCAC
AGACTCAAGTTACAGATTTGCACGTTCAATTGACCCTGAATTTACCCTTGATGGACTCAATCTTGCAGCCAAAATGATTT
TGGATTTATGTGGCGGAGAAGTGTCAAGCATAGTGTCTGCTGGCAATTTGAATAAGGCTGATACCAAGATAAATTTTGAT
TATCAAGATGCAAACAAACTTGGAAGTGTATCTGCATCACCTAATGAGCTATTCGATATTTTAACAAAGCTAGGGTTTAG
TATCGATAAAAAAACCGAAGGTAATTGGAATATACAAGTACCAAGCTGGAGACCAGACATAAAAATACCTGCTGACCTAG
TTGAAGAGGCAGTAAGAATATACGGCTATGATAAAATAAAGGAAGAGCAACTAGCAGAAGACGTTACAGTAGAAGAAGAG
ACACAAGATGATTTGCGCATTTTAATGACAAGCAGAGGATTTCATGAAGTGATAACTTGGTCTTTTATGAGTGAATCAAT
AGCTGAAAAGTTTGGTTACTTGAATAAGTTATTCATTATCGACAATCCATTTAATAATAACTTCAATATAATGAGGCCAA
GTGTCATACCAAATTTGTTGCAAGTCACTGCAGATAATATTGCTCACGGGATATCTGATCTCGCAATTTTTGAAATTGGA
CCAATTTATAATGGTGAGGCTCAGTCTAAGTACGTTTTAAGTGGAATTAGATCAGGAAATAATTCGCCGAAAAACCACTA
TAACACTGATAGAAAAATAGATATTTTTGACGCAAAGGCTGACCTCATAACAGCTTTGGAACTTTTTAATGTCAATTGTG
ATAATTTAGCAATAGAGAGAGCAGAAAAAGAATATTATCACCCAGGAAAATCAGGCTCCTTATCTTTTAGAAGTAAAATA
GTTGGTTATTTTGGAGAATTGCATCCGAGTATATTGGATTTTTTTAACATCAAACAAAAAATTGTAGGCTTTGAGTTGAT
ATTAGAAAATATCGAAAATTTACCTATAAGTAGAAAAAAATTTATCGATTATAAATATCAAAGTGTAAAACGCGACTTTG
CATTCATTGTAAATAAAGATGTAGCAGCAGGCAGTATAATCAATATGGTGAAAAAAAGCTCAGAGCTCATCACAGAAGTT
TTGATATTTGATGTATATCATGGAAACAATATAGAATCGAATAAGATGTCTATAGCATTTTCAGTTACTTTCTGCTCTCC
AACTCACACTTTAACTGAAGAGGAAATCCAAAAAGAATCAAATATGATAGTCAACTTAGTGTGTGAAAATACTGAAGGAA
CTTTGAGAGCTCATCATTAA

Upstream 100 bases:

>100_bases
TCTCCTTCGTTTTTTACAATTAGAGAAGCTACTCACTACAACAAGCTTTAAAATCATTATATTTTACATTACAATAACTT
TTTTATTTTCTGTTAAATAA

Downstream 100 bases:

>100_bases
GCCATAATCGCCGTTTCCTGATTTTGCTGTGTCACGTTTACTTCATCCAATTTATTTCCTATCTTGAATTCAATATCCTT
GAAAAACCTATCCCACATTC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVENVIDNSRLAGFVVAEVLEVAPHPNADKLKLCRVNDGSKVLQIVCGAK
NVKEGMKTVLASLGSTLPESNFTIKPTKIRGVLSEGMLCSASELSLTQDESEGIIELSDDYKTGDKFFNCDPVIDINITP
NRGDCLGVYGIARDLAATGIGTLKTLIIPQLTASMNSLINVKVIDGESFISGVYITNVKNKESPKWLKDKLESIGMRSIS
AIVDITNYIMISFGRPMHAYDAKKIEGELIVRKANDEEKFIGLNDKEYLLNKDISVISDDKNVHGIAGIMGSKYSECTLK
TTDIFLESAWFDPISITKSSRQLGISTDSSYRFARSIDPEFTLDGLNLAAKMILDLCGGEVSSIVSAGNLNKADTKINFD
YQDANKLGSVSASPNELFDILTKLGFSIDKKTEGNWNIQVPSWRPDIKIPADLVEEAVRIYGYDKIKEEQLAEDVTVEEE
TQDDLRILMTSRGFHEVITWSFMSESIAEKFGYLNKLFIIDNPFNNNFNIMRPSVIPNLLQVTADNIAHGISDLAIFEIG
PIYNGEAQSKYVLSGIRSGNNSPKNHYNTDRKIDIFDAKADLITALELFNVNCDNLAIERAEKEYYHPGKSGSLSFRSKI
VGYFGELHPSILDFFNIKQKIVGFELILENIENLPISRKKFIDYKYQSVKRDFAFIVNKDVAAGSIINMVKKSSELITEV
LIFDVYHGNNIESNKMSIAFSVTFCSPTHTLTEEEIQKESNMIVNLVCENTEGTLRAHH

Sequences:

>Translated_779_residues
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVENVIDNSRLAGFVVAEVLEVAPHPNADKLKLCRVNDGSKVLQIVCGAK
NVKEGMKTVLASLGSTLPESNFTIKPTKIRGVLSEGMLCSASELSLTQDESEGIIELSDDYKTGDKFFNCDPVIDINITP
NRGDCLGVYGIARDLAATGIGTLKTLIIPQLTASMNSLINVKVIDGESFISGVYITNVKNKESPKWLKDKLESIGMRSIS
AIVDITNYIMISFGRPMHAYDAKKIEGELIVRKANDEEKFIGLNDKEYLLNKDISVISDDKNVHGIAGIMGSKYSECTLK
TTDIFLESAWFDPISITKSSRQLGISTDSSYRFARSIDPEFTLDGLNLAAKMILDLCGGEVSSIVSAGNLNKADTKINFD
YQDANKLGSVSASPNELFDILTKLGFSIDKKTEGNWNIQVPSWRPDIKIPADLVEEAVRIYGYDKIKEEQLAEDVTVEEE
TQDDLRILMTSRGFHEVITWSFMSESIAEKFGYLNKLFIIDNPFNNNFNIMRPSVIPNLLQVTADNIAHGISDLAIFEIG
PIYNGEAQSKYVLSGIRSGNNSPKNHYNTDRKIDIFDAKADLITALELFNVNCDNLAIERAEKEYYHPGKSGSLSFRSKI
VGYFGELHPSILDFFNIKQKIVGFELILENIENLPISRKKFIDYKYQSVKRDFAFIVNKDVAAGSIINMVKKSSELITEV
LIFDVYHGNNIESNKMSIAFSVTFCSPTHTLTEEEIQKESNMIVNLVCENTEGTLRAHH
>Mature_779_residues
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVENVIDNSRLAGFVVAEVLEVAPHPNADKLKLCRVNDGSKVLQIVCGAK
NVKEGMKTVLASLGSTLPESNFTIKPTKIRGVLSEGMLCSASELSLTQDESEGIIELSDDYKTGDKFFNCDPVIDINITP
NRGDCLGVYGIARDLAATGIGTLKTLIIPQLTASMNSLINVKVIDGESFISGVYITNVKNKESPKWLKDKLESIGMRSIS
AIVDITNYIMISFGRPMHAYDAKKIEGELIVRKANDEEKFIGLNDKEYLLNKDISVISDDKNVHGIAGIMGSKYSECTLK
TTDIFLESAWFDPISITKSSRQLGISTDSSYRFARSIDPEFTLDGLNLAAKMILDLCGGEVSSIVSAGNLNKADTKINFD
YQDANKLGSVSASPNELFDILTKLGFSIDKKTEGNWNIQVPSWRPDIKIPADLVEEAVRIYGYDKIKEEQLAEDVTVEEE
TQDDLRILMTSRGFHEVITWSFMSESIAEKFGYLNKLFIIDNPFNNNFNIMRPSVIPNLLQVTADNIAHGISDLAIFEIG
PIYNGEAQSKYVLSGIRSGNNSPKNHYNTDRKIDIFDAKADLITALELFNVNCDNLAIERAEKEYYHPGKSGSLSFRSKI
VGYFGELHPSILDFFNIKQKIVGFELILENIENLPISRKKFIDYKYQSVKRDFAFIVNKDVAAGSIINMVKKSSELITEV
LIFDVYHGNNIESNKMSIAFSVTFCSPTHTLTEEEIQKESNMIVNLVCENTEGTLRAHH

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=554, Percent_Identity=23.2851985559567, Blast_Score=84, Evalue=6e-16,
Organism=Escherichia coli, GI1788006, Length=811, Percent_Identity=32.4290998766954, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86780; Mature: 86780

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVENVIDNSRLAGFVVAEVLEVAPHPNADK
CEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
LKLCRVNDGSKVLQIVCGAKNVKEGMKTVLASLGSTLPESNFTIKPTKIRGVLSEGMLCS
EEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCEEC
ASELSLTQDESEGIIELSDDYKTGDKFFNCDPVIDINITPNRGDCLGVYGIARDLAATGI
CCCCCCCCCCCCCEEEECCCCCCCCCEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCH
GTLKTLIIPQLTASMNSLINVKVIDGESFISGVYITNVKNKESPKWLKDKLESIGMRSIS
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHH
AIVDITNYIMISFGRPMHAYDAKKIEGELIVRKANDEEKFIGLNDKEYLLNKDISVISDD
HHHHHHHEEEEECCCCCCCCCCHHCCCEEEEEECCCCCCEECCCCCHHHHCCCCEEEECC
KNVHGIAGIMGSKYSECTLKTTDIFLESAWFDPISITKSSRQLGISTDSSYRFARSIDPE
CCCCCHHHHHCCCCCCEEEEHHHHEEECCCCCCEEEECCCCEECCCCCCCCEEHCCCCCC
FTLDGLNLAAKMILDLCGGEVSSIVSAGNLNKADTKINFDYQDANKLGSVSASPNELFDI
EEECHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEECCCCCHHHCCCCCCCHHHHHHH
LTKLGFSIDKKTEGNWNIQVPSWRPDIKIPADLVEEAVRIYGYDKIKEEQLAEDVTVEEE
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
TQDDLRILMTSRGFHEVITWSFMSESIAEKFGYLNKLFIIDNPFNNNFNIMRPSVIPNLL
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECHHHHHHHH
QVTADNIAHGISDLAIFEIGPIYNGEAQSKYVLSGIRSGNNSPKNHYNTDRKIDIFDAKA
HHHHHHHHHCHHHEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECHH
DLITALELFNVNCDNLAIERAEKEYYHPGKSGSLSFRSKIVGYFGELHPSILDFFNIKQK
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
IVGFELILENIENLPISRKKFIDYKYQSVKRDFAFIVNKDVAAGSIINMVKKSSELITEV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHH
LIFDVYHGNNIESNKMSIAFSVTFCSPTHTLTEEEIQKESNMIVNLVCENTEGTLRAHH
HHEEEECCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHCCCEEEEEEEECCCCCEEECC
>Mature Secondary Structure
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVENVIDNSRLAGFVVAEVLEVAPHPNADK
CEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
LKLCRVNDGSKVLQIVCGAKNVKEGMKTVLASLGSTLPESNFTIKPTKIRGVLSEGMLCS
EEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCEEC
ASELSLTQDESEGIIELSDDYKTGDKFFNCDPVIDINITPNRGDCLGVYGIARDLAATGI
CCCCCCCCCCCCCEEEECCCCCCCCCEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCH
GTLKTLIIPQLTASMNSLINVKVIDGESFISGVYITNVKNKESPKWLKDKLESIGMRSIS
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHH
AIVDITNYIMISFGRPMHAYDAKKIEGELIVRKANDEEKFIGLNDKEYLLNKDISVISDD
HHHHHHHEEEEECCCCCCCCCCHHCCCEEEEEECCCCCCEECCCCCHHHHCCCCEEEECC
KNVHGIAGIMGSKYSECTLKTTDIFLESAWFDPISITKSSRQLGISTDSSYRFARSIDPE
CCCCCHHHHHCCCCCCEEEEHHHHEEECCCCCCEEEECCCCEECCCCCCCCEEHCCCCCC
FTLDGLNLAAKMILDLCGGEVSSIVSAGNLNKADTKINFDYQDANKLGSVSASPNELFDI
EEECHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEECCCCCHHHCCCCCCCHHHHHHH
LTKLGFSIDKKTEGNWNIQVPSWRPDIKIPADLVEEAVRIYGYDKIKEEQLAEDVTVEEE
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
TQDDLRILMTSRGFHEVITWSFMSESIAEKFGYLNKLFIIDNPFNNNFNIMRPSVIPNLL
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECHHHHHHHH
QVTADNIAHGISDLAIFEIGPIYNGEAQSKYVLSGIRSGNNSPKNHYNTDRKIDIFDAKA
HHHHHHHHHCHHHEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECHH
DLITALELFNVNCDNLAIERAEKEYYHPGKSGSLSFRSKIVGYFGELHPSILDFFNIKQK
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
IVGFELILENIENLPISRKKFIDYKYQSVKRDFAFIVNKDVAAGSIINMVKKSSELITEV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHH
LIFDVYHGNNIESNKMSIAFSVTFCSPTHTLTEEEIQKESNMIVNLVCENTEGTLRAHH
HHEEEECCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHCCCEEEEEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA