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The map label for this gene is rlmE [H]

Identifier: 190571015

GI number: 190571015

Start: 660985

End: 661563

Strand: Reverse

Name: rlmE [H]

Synonym: WPa_0607

Alternate gene names: 190571015

Gene position: 661563-660985 (Counterclockwise)

Preceding gene: 190571016

Following gene: 190571014

Centisome position: 44.63

GC content: 33.51

Gene sequence:

>579_bases
TTGAATGATCAGTATGTGCAAAAAACCAATAAGGACGGTTATAGATCGCGCTCGGCATATAAGTTAATAGAAATAGACGA
TAAATTTAAACTACTCCAACAAGGGCAAAAAATTATTGATCTTGGCGCTTTTCCTGGTGGATGGTCACAAGTTGCATCTA
AGAAAGGAGCGAGTGTGATTGCTGTTGACATAAAACCAATAAACGCAATTAGTGGAGTAGAGTGTATACAGTGTGATATT
ATCAGCGAACTTGAAATTTTAAGAGAAAAATTCAAGGATCACAAATTTGATGTAATTTTATCTGATATGGCACCAGAATC
TTGCGGTTTAAAATCGTTAGATCATATCAGAATTATGCTTTTATGCGAAGCAGCGCTCAATTTTGCAAAGCATTTTTTGA
ATCATGATGGCAAATTTGTAGTAAAAATTTTTCAAGGAGAGTCTGATAAAGATTTCTGCAATGAGCTAAAAAAAATGTTC
AAAACAGTAAGATACTTCAAACCAAAGTCAAGTAGATCTGAATCTACAGAAATGTATTTAGTAAGTTTAGGCTTTATTGG
TGGCAACTATTCTGTATAG

Upstream 100 bases:

>100_bases
CAAAGATATAATCAATAATAAGTAGGTTATTAGTATAAAGACCAGAGTAAAAACAGCTAGAGGTAGAAAGTTATCTTCAA
CAAGATGGCTACATCGCCAT

Downstream 100 bases:

>100_bases
CATATACTTACAGAAACGTTGACGAATCTTATCCTATTAAACTGAAGATTAATAACGCTCGCACAAAAGCCCTTTTTCTT
GTTTAAATATTTATGATAAT

Product: ribosomal RNA large subunit methyltransferase J, putative

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNDQYVQKTNKDGYRSRSAYKLIEIDDKFKLLQQGQKIIDLGAFPGGWSQVASKKGASVIAVDIKPINAISGVECIQCDI
ISELEILREKFKDHKFDVILSDMAPESCGLKSLDHIRIMLLCEAALNFAKHFLNHDGKFVVKIFQGESDKDFCNELKKMF
KTVRYFKPKSSRSESTEMYLVSLGFIGGNYSV

Sequences:

>Translated_192_residues
MNDQYVQKTNKDGYRSRSAYKLIEIDDKFKLLQQGQKIIDLGAFPGGWSQVASKKGASVIAVDIKPINAISGVECIQCDI
ISELEILREKFKDHKFDVILSDMAPESCGLKSLDHIRIMLLCEAALNFAKHFLNHDGKFVVKIFQGESDKDFCNELKKMF
KTVRYFKPKSSRSESTEMYLVSLGFIGGNYSV
>Mature_192_residues
MNDQYVQKTNKDGYRSRSAYKLIEIDDKFKLLQQGQKIIDLGAFPGGWSQVASKKGASVIAVDIKPINAISGVECIQCDI
ISELEILREKFKDHKFDVILSDMAPESCGLKSLDHIRIMLLCEAALNFAKHFLNHDGKFVVKIFQGESDKDFCNELKKMF
KTVRYFKPKSSRSESTEMYLVSLGFIGGNYSV

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=207, Percent_Identity=31.8840579710145, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=37.17277486911, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=37.17277486911, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=37.17277486911, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI194097365, Length=190, Percent_Identity=35.2631578947368, Blast_Score=117, Evalue=5e-27,
Organism=Escherichia coli, GI1789569, Length=188, Percent_Identity=42.5531914893617, Blast_Score=149, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17554650, Length=192, Percent_Identity=35.9375, Blast_Score=123, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17553474, Length=193, Percent_Identity=34.7150259067358, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71987561, Length=185, Percent_Identity=33.5135135135135, Blast_Score=102, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=185, Percent_Identity=33.5135135135135, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=33.5135135135135, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=34.3434343434343, Blast_Score=115, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=35.7894736842105, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321302, Length=102, Percent_Identity=36.2745098039216, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356387, Length=205, Percent_Identity=33.1707317073171, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648639, Length=197, Percent_Identity=33.502538071066, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859957, Length=193, Percent_Identity=36.2694300518135, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.8282828282828, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 21731; Mature: 21731

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQYVQKTNKDGYRSRSAYKLIEIDDKFKLLQQGQKIIDLGAFPGGWSQVASKKGASVI
CCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCEEE
AVDIKPINAISGVECIQCDIISELEILREKFKDHKFDVILSDMAPESCGLKSLDHIRIML
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHH
LCEAALNFAKHFLNHDGKFVVKIFQGESDKDFCNELKKMFKTVRYFKPKSSRSESTEMYL
HHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VSLGFIGGNYSV
EEEEECCCCCCC
>Mature Secondary Structure
MNDQYVQKTNKDGYRSRSAYKLIEIDDKFKLLQQGQKIIDLGAFPGGWSQVASKKGASVI
CCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCEEE
AVDIKPINAISGVECIQCDIISELEILREKFKDHKFDVILSDMAPESCGLKSLDHIRIML
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHH
LCEAALNFAKHFLNHDGKFVVKIFQGESDKDFCNELKKMFKTVRYFKPKSSRSESTEMYL
HHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VSLGFIGGNYSV
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA