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The map label for this gene is purH [H]

Identifier: 190570958

GI number: 190570958

Start: 580764

End: 582284

Strand: Direct

Name: purH [H]

Synonym: WPa_0542

Alternate gene names: 190570958

Gene position: 580764-582284 (Clockwise)

Preceding gene: 190570955

Following gene: 190570959

Centisome position: 39.18

GC content: 33.73

Gene sequence:

>1521_bases
ATGAAAATTAAAAGAGCTTTAATATCAGTATACGATAAGACAAATATAATTGAACTTGCATCGTTTTTGATGCAGCAACA
AATAGAAATTCTCTCAACAGGGAATACTTATAAAGTGCTATCAGGTGCGGGAATAAAAACGCAAGAAGTTTCAGATTACA
CACAATTTCCAGAAATACTAGATGGCAGAGTGAAAACTTTACATCCTAAAATTCATGGAGGAATACTCTGTGATCGAAAA
AGGCACGAAAAGGAAATGCAGAATCTAGAAATTAAGCCGATAGATCTGCTTATAACTAACTTATATCCATTTTGGAAAAC
GGTTAATAGCAACTCAAGTGAGAAGCAAATCATAGAACAAATAGATATAGGTGGAGTAGCATTAATTAGAGCTACAGCAA
AAAACTTTCATTTCACTTCAGTGATTTCTAGCATTCAAGATTACGAAACACTGAAAGCTGAGATGATCAAAAATAACAAT
CAGACAACATTAGAATATAGAAAACATCTAGCAACTAAAGCATTTGCTCTGACTGCACAGTATGATTCTAATATTTACAA
TTGGTTTTTATCACAGGGTAAAAGCAATGAGTTACCAGAGTTTTTTACTTTATATGGATGTAAAGCACAAGGACTCAGAT
ATGGTGAAAATCCTCATCAAAAAGCTGCATTTTATAGCAATCAATTTAGCAAGTATCCACTGGAGAAAATACATGGAAAA
GAGTTGAGCTATAATAATATAGTCGACATAGAGTCTGCACTTAACATAATTTCTGAGTTTCAAGAACCTGCAGCAGTGAT
AATAAAGCACAATAATCCATGTGGCGCTGCTGTTAGTGATAGTGCTTTAAAAGCATATGAAAAAGCTCTATCATGTGATG
AAATAAGCAGTTTTGGTGGAATAGTTGCTTTCAATCGGGAGATAGATTTAAAGCTAGCAGAGAAATTAAACGAGATATTT
TTGGAAGTGATGATAGCACCATCGGTAAACAATGAGGCACTAAAAATTTTACAAAAAAAGAAAAATTTAAGAGTGATTAT
TCATCAATCTCTTCAGCAAAATGCAAAATATCAAATCAAAAATGTTGTTGGCGGGTTCTTAGTGCAAGAAAATAATAACC
ATACAGTAAAAGTAGAAGAAATAAACCGAGTGACAGAATGCACTACAACAAAAAAAGAGAAGGAAGATCTTATTTTTGCT
TGGAAAATATGTAAACATGTAAAATCCAATGCAATAGTGATAGCAAAAGACGGTTGTGCTATTGGCATCGGTGCAGGACA
AACAAGCAGAATAGATAGTGTGAATATTGCAGTAAAAAAAGCAGGTGAAAAATGTAAAGGCGCAGCGCTTGCTTCAGATG
CATTTTTTCCATTTCCAGATAGTATAGTAGAAAGTGCAAAGCATGGAATTACAGCTATAATTCAGCCAGGTGGCTCATTG
AAAGATCAAGACGTGATAGCAGCTGCAAATGAGAATAAAATTGCCATGTTTTTCACTGATGTTCGTAACTTTCTCCATTA
A

Upstream 100 bases:

>100_bases
TGTTAGCGGAATCTACAACGAGATACCGCAGTGGTATGACGTAGTAGCTATATATAGACAAATTTTCTTTTCTTAGATAT
AATCTTAAGAAACCTTCCAT

Downstream 100 bases:

>100_bases
GCTTTGTATCCTGTCAAAATTATACCCACACAGGGCTTTGTTGCATAGCAGCTTATAGGTATGAAAATTGTAGTAAATTT
ATGTAGTTACTTTAAACTCA

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKIKRALISVYDKTNIIELASFLMQQQIEILSTGNTYKVLSGAGIKTQEVSDYTQFPEILDGRVKTLHPKIHGGILCDRK
RHEKEMQNLEIKPIDLLITNLYPFWKTVNSNSSEKQIIEQIDIGGVALIRATAKNFHFTSVISSIQDYETLKAEMIKNNN
QTTLEYRKHLATKAFALTAQYDSNIYNWFLSQGKSNELPEFFTLYGCKAQGLRYGENPHQKAAFYSNQFSKYPLEKIHGK
ELSYNNIVDIESALNIISEFQEPAAVIIKHNNPCGAAVSDSALKAYEKALSCDEISSFGGIVAFNREIDLKLAEKLNEIF
LEVMIAPSVNNEALKILQKKKNLRVIIHQSLQQNAKYQIKNVVGGFLVQENNNHTVKVEEINRVTECTTTKKEKEDLIFA
WKICKHVKSNAIVIAKDGCAIGIGAGQTSRIDSVNIAVKKAGEKCKGAALASDAFFPFPDSIVESAKHGITAIIQPGGSL
KDQDVIAAANENKIAMFFTDVRNFLH

Sequences:

>Translated_506_residues
MKIKRALISVYDKTNIIELASFLMQQQIEILSTGNTYKVLSGAGIKTQEVSDYTQFPEILDGRVKTLHPKIHGGILCDRK
RHEKEMQNLEIKPIDLLITNLYPFWKTVNSNSSEKQIIEQIDIGGVALIRATAKNFHFTSVISSIQDYETLKAEMIKNNN
QTTLEYRKHLATKAFALTAQYDSNIYNWFLSQGKSNELPEFFTLYGCKAQGLRYGENPHQKAAFYSNQFSKYPLEKIHGK
ELSYNNIVDIESALNIISEFQEPAAVIIKHNNPCGAAVSDSALKAYEKALSCDEISSFGGIVAFNREIDLKLAEKLNEIF
LEVMIAPSVNNEALKILQKKKNLRVIIHQSLQQNAKYQIKNVVGGFLVQENNNHTVKVEEINRVTECTTTKKEKEDLIFA
WKICKHVKSNAIVIAKDGCAIGIGAGQTSRIDSVNIAVKKAGEKCKGAALASDAFFPFPDSIVESAKHGITAIIQPGGSL
KDQDVIAAANENKIAMFFTDVRNFLH
>Mature_506_residues
MKIKRALISVYDKTNIIELASFLMQQQIEILSTGNTYKVLSGAGIKTQEVSDYTQFPEILDGRVKTLHPKIHGGILCDRK
RHEKEMQNLEIKPIDLLITNLYPFWKTVNSNSSEKQIIEQIDIGGVALIRATAKNFHFTSVISSIQDYETLKAEMIKNNN
QTTLEYRKHLATKAFALTAQYDSNIYNWFLSQGKSNELPEFFTLYGCKAQGLRYGENPHQKAAFYSNQFSKYPLEKIHGK
ELSYNNIVDIESALNIISEFQEPAAVIIKHNNPCGAAVSDSALKAYEKALSCDEISSFGGIVAFNREIDLKLAEKLNEIF
LEVMIAPSVNNEALKILQKKKNLRVIIHQSLQQNAKYQIKNVVGGFLVQENNNHTVKVEEINRVTECTTTKKEKEDLIFA
WKICKHVKSNAIVIAKDGCAIGIGAGQTSRIDSVNIAVKKAGEKCKGAALASDAFFPFPDSIVESAKHGITAIIQPGGSL
KDQDVIAAANENKIAMFFTDVRNFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=470, Percent_Identity=36.3829787234043, Blast_Score=246, Evalue=3e-65,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=47.0363288718929, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71985564, Length=473, Percent_Identity=35.3065539112051, Blast_Score=231, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI71985574, Length=308, Percent_Identity=30.1948051948052, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323768, Length=471, Percent_Identity=34.6072186836518, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=34.2494714587738, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24649832, Length=473, Percent_Identity=35.0951374207188, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56538; Mature: 56538

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRALISVYDKTNIIELASFLMQQQIEILSTGNTYKVLSGAGIKTQEVSDYTQFPEIL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCHHHH
DGRVKTLHPKIHGGILCDRKRHEKEMQNLEIKPIDLLITNLYPFWKTVNSNSSEKQIIEQ
CCCCEEECCHHCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IDIGGVALIRATAKNFHFTSVISSIQDYETLKAEMIKNNNQTTLEYRKHLATKAFALTAQ
HCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEH
YDSNIYNWFLSQGKSNELPEFFTLYGCKAQGLRYGENPHQKAAFYSNQFSKYPLEKIHGK
HCCHHHHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCC
ELSYNNIVDIESALNIISEFQEPAAVIIKHNNPCGAAVSDSALKAYEKALSCDEISSFGG
CCCCCCEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHCCC
IVAFNREIDLKLAEKLNEIFLEVMIAPSVNNEALKILQKKKNLRVIIHQSLQQNAKYQIK
EEEECCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEHHHHHCCCCEEHH
NVVGGFLVQENNNHTVKVEEINRVTECTTTKKEKEDLIFAWKICKHVKSNAIVIAKDGCA
HHHCCEEEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCE
IGIGAGQTSRIDSVNIAVKKAGEKCKGAALASDAFFPFPDSIVESAKHGITAIIQPGGSL
EEECCCCCCCCCEEEEEEECCCCHHCCCEEECCCCCCCCHHHHHHHHCCCEEEECCCCCC
KDQDVIAAANENKIAMFFTDVRNFLH
CCCCEEEECCCCEEEEEEHHHHHHCC
>Mature Secondary Structure
MKIKRALISVYDKTNIIELASFLMQQQIEILSTGNTYKVLSGAGIKTQEVSDYTQFPEIL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCHHHH
DGRVKTLHPKIHGGILCDRKRHEKEMQNLEIKPIDLLITNLYPFWKTVNSNSSEKQIIEQ
CCCCEEECCHHCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IDIGGVALIRATAKNFHFTSVISSIQDYETLKAEMIKNNNQTTLEYRKHLATKAFALTAQ
HCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEH
YDSNIYNWFLSQGKSNELPEFFTLYGCKAQGLRYGENPHQKAAFYSNQFSKYPLEKIHGK
HCCHHHHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCC
ELSYNNIVDIESALNIISEFQEPAAVIIKHNNPCGAAVSDSALKAYEKALSCDEISSFGG
CCCCCCEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHCCC
IVAFNREIDLKLAEKLNEIFLEVMIAPSVNNEALKILQKKKNLRVIIHQSLQQNAKYQIK
EEEECCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEHHHHHCCCCEEHH
NVVGGFLVQENNNHTVKVEEINRVTECTTTKKEKEDLIFAWKICKHVKSNAIVIAKDGCA
HHHCCEEEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCE
IGIGAGQTSRIDSVNIAVKKAGEKCKGAALASDAFFPFPDSIVESAKHGITAIIQPGGSL
EEECCCCCCCCCEEEEEEECCCCHHCCCEEECCCCCCCCHHHHHHHHCCCEEEECCCCCC
KDQDVIAAANENKIAMFFTDVRNFLH
CCCCEEEECCCCEEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA