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The map label for this gene is murB

Identifier: 190570894

GI number: 190570894

Start: 509929

End: 510813

Strand: Reverse

Name: murB

Synonym: WPa_0473

Alternate gene names: 190570894

Gene position: 510813-509929 (Counterclockwise)

Preceding gene: 190570895

Following gene: 190570892

Centisome position: 34.46

GC content: 36.16

Gene sequence:

>885_bases
ATGCTTATAAGCTTACCTAAAGTATGTGGAATCTACCGTTATAATGTTTCAATGTCTAAAATGACTTGGTTAAATGTTGG
TGGCCAAGCTGATGTACTATTTAAGCCACGTGACATTGAAGATCTAATGTGCTTGATAAAAGATGCGGAGTTACCAATTA
GCGTTATCGGTGCAACATCCAACATAATAATTCGAGATAGTGGCATTCGAGGAATAACGGTGAAATTAGGTAAGGAATTT
GCGTATATTAAATGTAAAGATAATAGCTCCATAGTTGCAGGTGGTGCTGCACTGCTTAGTAACCTTGCCTACTTTGCAGG
GGAACAGCAAATTAGTGGGCTTGAGTTTCTTGCTGGAATTCCAGGAACAGTTGGCGGTGGAATAGAAATGAATGCAGGTG
CATATGGTAGTGATATTGCGAGTGTTGTAAAATTTATAAGGGCAGTGAATCTAGAAGATGGAAATTTATATGAATTCTCC
AGCGAAGAAATGGGATATTTTTATCGTGGACATAGTCTAAAAGGCAGGTGGATTTTTATTGAAGCTGAATTTAAAGGAGT
AAGTTCAGAGTATGAGCTTATATTGCAAAGATTGAAAGAAGTTATTGATAAAAAAAATAAAAGTCAACCAGTAAGAGGAA
AAACTGCTGGTTGTATATTCAAAAATCCAATAGGCTGCAAGGCATGGAAACTGATTGATGAATCTGGCTGCCGAGGATTA
GATAATGGTGTAGCTAAAATTTCTAAGAAACATTGTAATTTTCTACTCAATTACAATAATGCAACTGCACTTGACTTAGA
AAACCTTGGCAACAGAGTAAAAGATGCAGTAAAAGATAAATTTAACATTGAACTTGAGTGGGAGATAAGGGTTTTGGGTA
GGTAA

Upstream 100 bases:

>100_bases
TGTTAGCGGGCGAGAGAGTTATATACTTTACTGAACGCACTTCGTTTGTAGAAGATGCAGAAAAAATGGGTATGGATGCA
GGATTAGAGTTAAAATCAAA

Downstream 100 bases:

>100_bases
CTTCTAACCTTCTCTAATATGACCTATTTCAATTGAGTACACTTCAATTTAAGTCCAAGGTACACTATTTTGCGAGGATA
ATTTATGCTTCCTTTGAAGA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLISLPKVCGIYRYNVSMSKMTWLNVGGQADVLFKPRDIEDLMCLIKDAELPISVIGATSNIIIRDSGIRGITVKLGKEF
AYIKCKDNSSIVAGGAALLSNLAYFAGEQQISGLEFLAGIPGTVGGGIEMNAGAYGSDIASVVKFIRAVNLEDGNLYEFS
SEEMGYFYRGHSLKGRWIFIEAEFKGVSSEYELILQRLKEVIDKKNKSQPVRGKTAGCIFKNPIGCKAWKLIDESGCRGL
DNGVAKISKKHCNFLLNYNNATALDLENLGNRVKDAVKDKFNIELEWEIRVLGR

Sequences:

>Translated_294_residues
MLISLPKVCGIYRYNVSMSKMTWLNVGGQADVLFKPRDIEDLMCLIKDAELPISVIGATSNIIIRDSGIRGITVKLGKEF
AYIKCKDNSSIVAGGAALLSNLAYFAGEQQISGLEFLAGIPGTVGGGIEMNAGAYGSDIASVVKFIRAVNLEDGNLYEFS
SEEMGYFYRGHSLKGRWIFIEAEFKGVSSEYELILQRLKEVIDKKNKSQPVRGKTAGCIFKNPIGCKAWKLIDESGCRGL
DNGVAKISKKHCNFLLNYNNATALDLENLGNRVKDAVKDKFNIELEWEIRVLGR
>Mature_294_residues
MLISLPKVCGIYRYNVSMSKMTWLNVGGQADVLFKPRDIEDLMCLIKDAELPISVIGATSNIIIRDSGIRGITVKLGKEF
AYIKCKDNSSIVAGGAALLSNLAYFAGEQQISGLEFLAGIPGTVGGGIEMNAGAYGSDIASVVKFIRAVNLEDGNLYEFS
SEEMGYFYRGHSLKGRWIFIEAEFKGVSSEYELILQRLKEVIDKKNKSQPVRGKTAGCIFKNPIGCKAWKLIDESGCRGL
DNGVAKISKKHCNFLLNYNNATALDLENLGNRVKDAVKDKFNIELEWEIRVLGR

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_WOLPP (B3CPS8)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975252.1
- EnsemblBacteria:   EBWOLT00000000462
- GeneID:   6385136
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0473
- GeneTree:   EBGT00050000030840
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- ProtClustDB:   PRK13905
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32381; Mature: 32381

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 169-169 ACT_SITE 218-218 ACT_SITE 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISLPKVCGIYRYNVSMSKMTWLNVGGQADVLFKPRDIEDLMCLIKDAELPISVIGATS
CEECCCHHHCEEEECCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCC
NIIIRDSGIRGITVKLGKEFAYIKCKDNSSIVAGGAALLSNLAYFAGEQQISGLEFLAGI
EEEEEECCCCEEEEEECCEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHCC
PGTVGGGIEMNAGAYGSDIASVVKFIRAVNLEDGNLYEFSSEEMGYFYRGHSLKGRWIFI
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCCEEEEE
EAEFKGVSSEYELILQRLKEVIDKKNKSQPVRGKTAGCIFKNPIGCKAWKLIDESGCRGL
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHEEEECCCCCCCC
DNGVAKISKKHCNFLLNYNNATALDLENLGNRVKDAVKDKFNIELEWEIRVLGR
HHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHEEEEEEEEEEEECC
>Mature Secondary Structure
MLISLPKVCGIYRYNVSMSKMTWLNVGGQADVLFKPRDIEDLMCLIKDAELPISVIGATS
CEECCCHHHCEEEECCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCC
NIIIRDSGIRGITVKLGKEFAYIKCKDNSSIVAGGAALLSNLAYFAGEQQISGLEFLAGI
EEEEEECCCCEEEEEECCEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHCC
PGTVGGGIEMNAGAYGSDIASVVKFIRAVNLEDGNLYEFSSEEMGYFYRGHSLKGRWIFI
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCCEEEEE
EAEFKGVSSEYELILQRLKEVIDKKNKSQPVRGKTAGCIFKNPIGCKAWKLIDESGCRGL
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHEEEECCCCCCCC
DNGVAKISKKHCNFLLNYNNATALDLENLGNRVKDAVKDKFNIELEWEIRVLGR
HHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA