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The map label for this gene is pleD [H]

Identifier: 190570797

GI number: 190570797

Start: 383131

End: 384507

Strand: Direct

Name: pleD [H]

Synonym: WPa_0358

Alternate gene names: 190570797

Gene position: 383131-384507 (Clockwise)

Preceding gene: 190570796

Following gene: 190570799

Centisome position: 25.84

GC content: 34.28

Gene sequence:

>1377_bases
ATGACAGCAAAAATCCTAGTAGTAGATGATATACCATCTAATGTTAAGCTTTTGGAGGCTCAGCTAAAAGCAGAGTACTA
TACAGTTATTGTAGCTTATGATGGCGAGGAGGCGATAGATTTAGTAGCGGAACAGCAGCCAGATATTATCTTACTTGATG
TCATGATGCCAAAAATGAGTGGTTTTGAGGTTTGTAAGAAGCTAAAGAATGATCCCTTAACGACTTATATTCCAATCATT
ATGGTAACTGCGCTGCATGACACTCATGATAGAGTGGAAGGAATTAATGCTGGTGCGGATGACTTTCTAACTAAGCCGAT
AGATGAAACTGCTTTATCTGCAAGAATTAAGTCTCTTGTGCGCTTAAAGATGATAATAGATGAGTTGTGCTTGAGAGGAG
AAACTAATGCTGAGATTGGTGGAGTAGCAGAAAGCAGTATTATGGATTATTCTAATCAGATTTTTGATGCTAACATACTT
GTTGTAGATGAGGACACCTTTCAAGCAGAGCAGATACACAATGTTCTAAAGCAACGCTTCAGATCAATTAAAATACTGCA
CGATCCAAAAGAAGCATTAAAGGTTGGTATTAAGGATAATTACGACCTGATTATTTCTGACATGCAGTTTTCAAAAACTG
ACGGCCTACGGTTGTGTTCTAAGTTTCGTAGTAAAGTAGAAACACGTTATACACCAATTCTGATCCTTTCTGAAGATTAT
GATAAAAGCAATTTAGTAAAGGCACTTGATGTAGGTGCTAATGATTATTTAACAGTACCTTTAGATGAGAGTGAATTAAT
AGCTAGGGTTAATTTGCAAGTGAAACGCAAAAGGTATCAAGATGCCTTGAGAATGAATTTATTTAATAATGTAGAAATGT
CTATAAAGGATCCATTAACTAACTGTTATAATAGAAGATATTTTGATGCACACTTAAGAAATATTGTTAAGGATTCTGTG
GAAAAAGATAGAAGTTTATCTCTTATGATACTTGATATAGATTATTTTAAGATGGTGAATGATAACTTTGGGCATAATGC
TGGAGATGAACTTTTAAAGGAAATACAGAAAAGAATTTCTGAAAATATCAGAGTGACAGATTTATTAGCTAGGTTTGGTG
GTGAGGAATTTGTTGTTGTAATGCCAGATACCAATGTATCAGATGCGTATACTATTGCAGAGAGAATAAGGAAAATTATT
GCCAAAGAACCTTTTATACTTGTGGGTAAAAATACAACCCATAATGTAACTGTGAGCATTGGAATTTCAGCAATGCAAAG
ATCTGACCTTGATGATGTTAAAAAATTTATAGTACGTGCTGACAAATATTTATATAAAGCAAAAAACAGTGGTAGAAATA
GAGTGGTTACTGGTTGA

Upstream 100 bases:

>100_bases
ATTAAAGAATTATATGAAGGGCATAACGTTGAAAAAGTCAATTACATAATTGGTGAAGATGGCAAGCTTTATCCTACTTA
CAATGTAGATAGTACAAAAA

Downstream 100 bases:

>100_bases
AGTTCTTTCAAATAAGTAGTAACTAAGATTCAAATGATAGCATAAAATTTCTCAACTCCGGAAATTTCGCATTTAACTGC
AGAAATAGATATTTATTTAA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTAKILVVDDIPSNVKLLEAQLKAEYYTVIVAYDGEEAIDLVAEQQPDIILLDVMMPKMSGFEVCKKLKNDPLTTYIPII
MVTALHDTHDRVEGINAGADDFLTKPIDETALSARIKSLVRLKMIIDELCLRGETNAEIGGVAESSIMDYSNQIFDANIL
VVDEDTFQAEQIHNVLKQRFRSIKILHDPKEALKVGIKDNYDLIISDMQFSKTDGLRLCSKFRSKVETRYTPILILSEDY
DKSNLVKALDVGANDYLTVPLDESELIARVNLQVKRKRYQDALRMNLFNNVEMSIKDPLTNCYNRRYFDAHLRNIVKDSV
EKDRSLSLMILDIDYFKMVNDNFGHNAGDELLKEIQKRISENIRVTDLLARFGGEEFVVVMPDTNVSDAYTIAERIRKII
AKEPFILVGKNTTHNVTVSIGISAMQRSDLDDVKKFIVRADKYLYKAKNSGRNRVVTG

Sequences:

>Translated_458_residues
MTAKILVVDDIPSNVKLLEAQLKAEYYTVIVAYDGEEAIDLVAEQQPDIILLDVMMPKMSGFEVCKKLKNDPLTTYIPII
MVTALHDTHDRVEGINAGADDFLTKPIDETALSARIKSLVRLKMIIDELCLRGETNAEIGGVAESSIMDYSNQIFDANIL
VVDEDTFQAEQIHNVLKQRFRSIKILHDPKEALKVGIKDNYDLIISDMQFSKTDGLRLCSKFRSKVETRYTPILILSEDY
DKSNLVKALDVGANDYLTVPLDESELIARVNLQVKRKRYQDALRMNLFNNVEMSIKDPLTNCYNRRYFDAHLRNIVKDSV
EKDRSLSLMILDIDYFKMVNDNFGHNAGDELLKEIQKRISENIRVTDLLARFGGEEFVVVMPDTNVSDAYTIAERIRKII
AKEPFILVGKNTTHNVTVSIGISAMQRSDLDDVKKFIVRADKYLYKAKNSGRNRVVTG
>Mature_457_residues
TAKILVVDDIPSNVKLLEAQLKAEYYTVIVAYDGEEAIDLVAEQQPDIILLDVMMPKMSGFEVCKKLKNDPLTTYIPIIM
VTALHDTHDRVEGINAGADDFLTKPIDETALSARIKSLVRLKMIIDELCLRGETNAEIGGVAESSIMDYSNQIFDANILV
VDEDTFQAEQIHNVLKQRFRSIKILHDPKEALKVGIKDNYDLIISDMQFSKTDGLRLCSKFRSKVETRYTPILILSEDYD
KSNLVKALDVGANDYLTVPLDESELIARVNLQVKRKRYQDALRMNLFNNVEMSIKDPLTNCYNRRYFDAHLRNIVKDSVE
KDRSLSLMILDIDYFKMVNDNFGHNAGDELLKEIQKRISENIRVTDLLARFGGEEFVVVMPDTNVSDAYTIAERIRKIIA
KEPFILVGKNTTHNVTVSIGISAMQRSDLDDVKKFIVRADKYLYKAKNSGRNRVVTG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=201, Percent_Identity=37.3134328358209, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=38.0368098159509, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=41.2121212121212, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786584, Length=196, Percent_Identity=32.6530612244898, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=37.1069182389937, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=35.195530726257, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=35.1851851851852, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=38.8429752066116, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788394, Length=213, Percent_Identity=30.5164319248826, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.9397590361446, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367329, Length=118, Percent_Identity=45.7627118644068, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=35.5932203389831, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=147, Percent_Identity=35.3741496598639, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=30.4878048780488, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=175, Percent_Identity=29.7142857142857, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=30.5084745762712, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=32.2033898305085, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=29.7709923664122, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=32.1428571428571, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=113, Percent_Identity=30.9734513274336, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=36.8932038834951, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=34.4827586206897, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787541, Length=148, Percent_Identity=31.0810810810811, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=31.3559322033898, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52057; Mature: 51925

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKILVVDDIPSNVKLLEAQLKAEYYTVIVAYDGEEAIDLVAEQQPDIILLDVMMPKMS
CCEEEEEEECCCCCHHHHHHHHCCEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCC
GFEVCKKLKNDPLTTYIPIIMVTALHDTHDRVEGINAGADDFLTKPIDETALSARIKSLV
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHH
RLKMIIDELCLRGETNAEIGGVAESSIMDYSNQIFDANILVVDEDTFQAEQIHNVLKQRF
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHH
RSIKILHDPKEALKVGIKDNYDLIISDMQFSKTDGLRLCSKFRSKVETRYTPILILSEDY
HHEEEEECCHHHHEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
DKSNLVKALDVGANDYLTVPLDESELIARVNLQVKRKRYQDALRMNLFNNVEMSIKDPLT
CCHHHHHHEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHH
NCYNRRYFDAHLRNIVKDSVEKDRSLSLMILDIDYFKMVNDNFGHNAGDELLKEIQKRIS
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHH
ENIRVTDLLARFGGEEFVVVMPDTNVSDAYTIAERIRKIIAKEPFILVGKNTTHNVTVSI
CCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEE
GISAMQRSDLDDVKKFIVRADKYLYKAKNSGRNRVVTG
EEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAKILVVDDIPSNVKLLEAQLKAEYYTVIVAYDGEEAIDLVAEQQPDIILLDVMMPKMS
CEEEEEEECCCCCHHHHHHHHCCEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCC
GFEVCKKLKNDPLTTYIPIIMVTALHDTHDRVEGINAGADDFLTKPIDETALSARIKSLV
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHH
RLKMIIDELCLRGETNAEIGGVAESSIMDYSNQIFDANILVVDEDTFQAEQIHNVLKQRF
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHH
RSIKILHDPKEALKVGIKDNYDLIISDMQFSKTDGLRLCSKFRSKVETRYTPILILSEDY
HHEEEEECCHHHHEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
DKSNLVKALDVGANDYLTVPLDESELIARVNLQVKRKRYQDALRMNLFNNVEMSIKDPLT
CCHHHHHHEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHH
NCYNRRYFDAHLRNIVKDSVEKDRSLSLMILDIDYFKMVNDNFGHNAGDELLKEIQKRIS
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHH
ENIRVTDLLARFGGEEFVVVMPDTNVSDAYTIAERIRKIIAKEPFILVGKNTTHNVTVSI
CCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEE
GISAMQRSDLDDVKKFIVRADKYLYKAKNSGRNRVVTG
EEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA