Click here to switch to the map view.

The map label for this gene is fucA [C]

Identifier: 190570645

GI number: 190570645

Start: 197012

End: 197722

Strand: Direct

Name: fucA [C]

Synonym: WPa_0187

Alternate gene names: 190570645

Gene position: 197012-197722 (Clockwise)

Preceding gene: 190570643

Following gene: 190570647

Centisome position: 13.29

GC content: 33.47

Gene sequence:

>711_bases
ATGTTATTAACAAATAGTGAGTTGAAAAGAGACCTAGTTAACGCCTATCAAATTTTATCTTATCTTAAATTAGATGATCA
TACGTATACTCATCTCTCTGTACGTTCTGAAGATAAAAAGTCATTTTATGTTTATCCGTTTGGTATACGTTTTTATGAAG
TAGATGAAAGTTCATTGATGAAAGTATCGTTTGATGGGAATATAATTGAAGGCAAAGAATATCAATATAATAAAACTGGC
TATGTAATCCATGGCTTCATTTATCAAGCAAGGAAAGATATTCAAGCAATTTTTCATCTACATACACCTTCTATTGTAGC
AGTTTCTTCTCTCAAGGATGGATTACTCCCAATAAGTCAGTGGGCGCTGCACTTTTATAATAAGGTATCTTACCATGATT
ATAATTCCCTGGCACTTGATGATACAGAAGGAAAAAGATTAATAGCTGATTTGAAAGAAAATTTTGTGATGTTAATGCGC
AATCATGGATCTATAACATGTGGTCAGACTATACAAGAAGCAATGTTTTATACGTATCACTTAGAACAAGCTTGTAAAAC
TCAATGCCTGATGCTAGCAATGAATAGGGAGTTGTCAATTCCAAGTGAAGAAATTTGCTCAAAAGCCGTAAAGGATCTTC
TGTCTTTCGAAAGCAATCTTGGTGAGAGAGATTGGCACGCATGGGTTAGGTTGATTAAAGGTAAGTTATAA

Upstream 100 bases:

>100_bases
GGCAAGTAAAAGTGCTTAGTATTTTAACGTAGGTTCACCTTTTAGTTTGTAATAAACTTTTTTTTACGTATAAATCTTTA
GAAAATTTTAGTCAAGAGCT

Downstream 100 bases:

>100_bases
AAGTTTCTGACTCTTCGATAGATATTAGATTTCTTGCATAACCAGCCAGTCTGAAATCCAATAATGGTTTCACACGCTGT
AATTCATGCAAGAAGTCTAA

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLLTNSELKRDLVNAYQILSYLKLDDHTYTHLSVRSEDKKSFYVYPFGIRFYEVDESSLMKVSFDGNIIEGKEYQYNKTG
YVIHGFIYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQWALHFYNKVSYHDYNSLALDDTEGKRLIADLKENFVMLMR
NHGSITCGQTIQEAMFYTYHLEQACKTQCLMLAMNRELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKGKL

Sequences:

>Translated_236_residues
MLLTNSELKRDLVNAYQILSYLKLDDHTYTHLSVRSEDKKSFYVYPFGIRFYEVDESSLMKVSFDGNIIEGKEYQYNKTG
YVIHGFIYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQWALHFYNKVSYHDYNSLALDDTEGKRLIADLKENFVMLMR
NHGSITCGQTIQEAMFYTYHLEQACKTQCLMLAMNRELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKGKL
>Mature_236_residues
MLLTNSELKRDLVNAYQILSYLKLDDHTYTHLSVRSEDKKSFYVYPFGIRFYEVDESSLMKVSFDGNIIEGKEYQYNKTG
YVIHGFIYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQWALHFYNKVSYHDYNSLALDDTEGKRLIADLKENFVMLMR
NHGSITCGQTIQEAMFYTYHLEQACKTQCLMLAMNRELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKGKL

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826321, Length=190, Percent_Identity=34.2105263157895, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI29826319, Length=190, Percent_Identity=34.2105263157895, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI29826325, Length=169, Percent_Identity=36.6863905325444, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI29826323, Length=169, Percent_Identity=36.6863905325444, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI297307160, Length=176, Percent_Identity=35.7954545454545, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI89276787, Length=176, Percent_Identity=35.7954545454545, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI9257190, Length=176, Percent_Identity=35.7954545454545, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI297307158, Length=186, Percent_Identity=34.9462365591398, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI9257192, Length=186, Percent_Identity=34.9462365591398, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI9943848, Length=168, Percent_Identity=34.5238095238095, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI9951927, Length=168, Percent_Identity=34.5238095238095, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI62912453, Length=168, Percent_Identity=34.5238095238095, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533218, Length=241, Percent_Identity=33.195020746888, Blast_Score=145, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115533216, Length=183, Percent_Identity=39.344262295082, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71989541, Length=217, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71989550, Length=217, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71989555, Length=217, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71989560, Length=217, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71989544, Length=217, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281363763, Length=161, Percent_Identity=37.2670807453416, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363757, Length=161, Percent_Identity=37.2670807453416, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136738, Length=161, Percent_Identity=37.2670807453416, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363755, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363751, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655690, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655685, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363761, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281363759, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281363753, Length=161, Percent_Identity=37.2670807453416, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 27416; Mature: 27416

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTNSELKRDLVNAYQILSYLKLDDHTYTHLSVRSEDKKSFYVYPFGIRFYEVDESSLM
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEEEEEECCCCEE
KVSFDGNIIEGKEYQYNKTGYVIHGFIYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQ
EEEECCCEECCCCEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCHHHH
WALHFYNKVSYHDYNSLALDDTEGKRLIADLKENFVMLMRNHGSITCGQTIQEAMFYTYH
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHH
LEQACKTQCLMLAMNRELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKGKL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLTNSELKRDLVNAYQILSYLKLDDHTYTHLSVRSEDKKSFYVYPFGIRFYEVDESSLM
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEEEEEECCCCEE
KVSFDGNIIEGKEYQYNKTGYVIHGFIYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQ
EEEECCCEECCCCEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCHHHH
WALHFYNKVSYHDYNSLALDDTEGKRLIADLKENFVMLMRNHGSITCGQTIQEAMFYTYH
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHH
LEQACKTQCLMLAMNRELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKGKL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]