Click here to switch to the map view.

The map label for this gene is ispE [H]

Identifier: 190570632

GI number: 190570632

Start: 181496

End: 182362

Strand: Reverse

Name: ispE [H]

Synonym: WPa_0174

Alternate gene names: 190570632

Gene position: 182362-181496 (Counterclockwise)

Preceding gene: 190570634

Following gene: 190570631

Centisome position: 12.3

GC content: 36.33

Gene sequence:

>867_bases
ATGGAAAGTTTTTGTGTAAAGGCTCCTGCAAAGATCAATCTTTTTTTACATATTATAAATAGAGAAGAAAGAGGGTATCA
TTTAATTGAAAGTTTGGTTGTCTTTGCTAAACTTTCTAATTTTTTAGAGATAAAAGTAGGAGAAAAAAACTTTAGATATG
ATAACTCTACAGTTGAATTTGTAAATTCTGAGTTTAGAATAAATTATCGATATAATACTATAACGAGAGCAGTGAGTCTA
CTGCTTAGGCATGCTCCTGTACGCACTAAAGTCACTGTGAAGGTTGTAAAAAATGTACCAATTGCTGCGGGATTTGGTAG
TGGTTCCTCAGATGCTGGAGCTGTGATACGCATGCTGGGAGAGCTTTGGAACATTGATAGGCAATTTTTAAATGATATAG
CTTTAAGCGTTGGTGCTGATGTACCTGCAAGTATAGATAGTAAGCCAGTTTTTGTCAGAGGTGTTGGTGAGGAATTATGC
CCCATTAAACAATGCTCTTTACCCACAAGTCTGGTACTTGTGAAACCGAAAAAAAAGTTTTTGAATACATCAGAAGTATT
TTCCAAGTATAAAGAGGATTTCTCTCAGCCAATTAAGTGGAGTGATAACACTGAGGAGAATTTGTTAGAGCTTCTTAAAG
AGACAAAAAATGACCTGCAAGAAGCAGCAATAAGTCTTGTACCTGAAATTCAGAATGTAATATCAACACTAGAGTCACAA
GAAGGCTCTATACTTTCTCGTATGTCAGGTAGTGGTGTAGGGTGTTTTGGAATGTTTGATAGTGAAGAAAGTGCAAAAGC
TGCAGCGGCAAATATCAAAAAGGAATATCCAGAATGGTGGGTGTGCGACACGGAATTAATGGTTTGA

Upstream 100 bases:

>100_bases
TTAGGATTTATGGCATTTGATCTCATTAGGAGTGTATAAAGTTTGACTGAAGTGGCATTTCAAGTATAGTAAATAAGATA
GTGTTAGTGAAGATATACAG

Downstream 100 bases:

>100_bases
TTTATGCCCTTAATTTCCAAGAAGAAAAATCTTATAGATGGTTACAGGGAAGAAAGATTTTGGGTGAATAATCAAGAATA
TCATGGCTCAATTATAATTT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MESFCVKAPAKINLFLHIINREERGYHLIESLVVFAKLSNFLEIKVGEKNFRYDNSTVEFVNSEFRINYRYNTITRAVSL
LLRHAPVRTKVTVKVVKNVPIAAGFGSGSSDAGAVIRMLGELWNIDRQFLNDIALSVGADVPASIDSKPVFVRGVGEELC
PIKQCSLPTSLVLVKPKKKFLNTSEVFSKYKEDFSQPIKWSDNTEENLLELLKETKNDLQEAAISLVPEIQNVISTLESQ
EGSILSRMSGSGVGCFGMFDSEESAKAAAANIKKEYPEWWVCDTELMV

Sequences:

>Translated_288_residues
MESFCVKAPAKINLFLHIINREERGYHLIESLVVFAKLSNFLEIKVGEKNFRYDNSTVEFVNSEFRINYRYNTITRAVSL
LLRHAPVRTKVTVKVVKNVPIAAGFGSGSSDAGAVIRMLGELWNIDRQFLNDIALSVGADVPASIDSKPVFVRGVGEELC
PIKQCSLPTSLVLVKPKKKFLNTSEVFSKYKEDFSQPIKWSDNTEENLLELLKETKNDLQEAAISLVPEIQNVISTLESQ
EGSILSRMSGSGVGCFGMFDSEESAKAAAANIKKEYPEWWVCDTELMV
>Mature_288_residues
MESFCVKAPAKINLFLHIINREERGYHLIESLVVFAKLSNFLEIKVGEKNFRYDNSTVEFVNSEFRINYRYNTITRAVSL
LLRHAPVRTKVTVKVVKNVPIAAGFGSGSSDAGAVIRMLGELWNIDRQFLNDIALSVGADVPASIDSKPVFVRGVGEELC
PIKQCSLPTSLVLVKPKKKFLNTSEVFSKYKEDFSQPIKWSDNTEENLLELLKETKNDLQEAAISLVPEIQNVISTLESQ
EGSILSRMSGSGVGCFGMFDSEESAKAAAANIKKEYPEWWVCDTELMV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=280, Percent_Identity=28.2142857142857, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 32178; Mature: 32178

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESFCVKAPAKINLFLHIINREERGYHLIESLVVFAKLSNFLEIKVGEKNFRYDNSTVEF
CCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEE
VNSEFRINYRYNTITRAVSLLLRHAPVRTKVTVKVVKNVPIAAGFGSGSSDAGAVIRMLG
ECCEEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHH
ELWNIDRQFLNDIALSVGADVPASIDSKPVFVRGVGEELCPIKQCSLPTSLVLVKPKKKF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCCEEEEECCHHHH
LNTSEVFSKYKEDFSQPIKWSDNTEENLLELLKETKNDLQEAAISLVPEIQNVISTLESQ
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGSILSRMSGSGVGCFGMFDSEESAKAAAANIKKEYPEWWVCDTELMV
CCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCEECC
>Mature Secondary Structure
MESFCVKAPAKINLFLHIINREERGYHLIESLVVFAKLSNFLEIKVGEKNFRYDNSTVEF
CCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEE
VNSEFRINYRYNTITRAVSLLLRHAPVRTKVTVKVVKNVPIAAGFGSGSSDAGAVIRMLG
ECCEEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHH
ELWNIDRQFLNDIALSVGADVPASIDSKPVFVRGVGEELCPIKQCSLPTSLVLVKPKKKF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCCEEEEECCHHHH
LNTSEVFSKYKEDFSQPIKWSDNTEENLLELLKETKNDLQEAAISLVPEIQNVISTLESQ
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGSILSRMSGSGVGCFGMFDSEESAKAAAANIKKEYPEWWVCDTELMV
CCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA