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The map label for this gene is miaA

Identifier: 190570597

GI number: 190570597

Start: 140824

End: 141717

Strand: Direct

Name: miaA

Synonym: WPa_0135

Alternate gene names: 190570597

Gene position: 140824-141717 (Clockwise)

Preceding gene: 190570591

Following gene: 190570598

Centisome position: 9.5

GC content: 34.0

Gene sequence:

>894_bases
ATGAAAGATAATATAGTAATTATTACAGGAATTACAGCCTCAGGTAAATCAGAATTATGCGATAACTTGATAAAAAAGCA
TAAAAACATTAGCATAATAAACTGTGACTCAAAGCAGGTATACAAAGAGATTCCCGTAATTACTGCTCAACCACCAAAGC
AGAAAGAATTTTATAGGCTATACGGTTATGTTTCAGCAAGAGAAAATTATTCCGTAGGTTTATGGCTAGAGGATTTAAAG
AGGGAAGTTAACAACGCGCTGAAAAATTCACGAATACCGATCATCACTGGAGGAAGTGGGCTTTACATTAGCAGCTTAAT
CAAGGGATTATCATCGATTCCACAAATAAGCCAGGAAGTGAGAGAAAACGTAAATGAACTTAGGAAAAATTTAAGTAAAG
AGGAATTCTACAAATTAGTGCTAAGCAAAGATCCAAGGATTCAAGGTAAAATATTTATTAATGACTCACATCGCCTCTCA
AGAGCACTTGAAGTTATCACTGAAACTGGCAAGACAATCTTTGTATGGCAAGAAAATAGACAGCCTCCTTTGTTCAATAA
TTTTAAGGTATATACAATTCTACCTAAACGTGAAGATATATACCGAAAAATAAATTCTCGTTTTATTGAAATGGTTGAAA
ATGGAGCAATTGATGAAGTAAAAAACCTACTTAGCATGAACCCATCTCCACATTTGCCAGCTATGAAAGCACATGGAGTA
CCAGAAATTATAAGATACTTAAAAGGGGAGATTACTTTAGACGAAGCAATACAGATTGCTCAAACAAACACAAGGCATTA
TGCAAAACGCCAGTACACTTGGTTTAAAAATCAATTTCCGAATTCTCAAGTGATTGACTGTGCAAATAAATTGACAGAAT
TTGGAATATTTTGA

Upstream 100 bases:

>100_bases
AGTTATCGTTATCATTTTCTGCCATAAAAGTCTCCAAATTTTAGATTGTAGAATCCAAATTTTTACTACACCCTATCATA
GGTTAGTTAAATTAATATAA

Downstream 100 bases:

>100_bases
TTATTATGTATTATATTGCTATGCCTATGCAAACTTTTCTTGATAGTATTAAACTTTTTTCTTAAAGAACTTTCATCATT
GCCTAAATTGTGATTATTAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKDNIVIITGITASGKSELCDNLIKKHKNISIINCDSKQVYKEIPVITAQPPKQKEFYRLYGYVSARENYSVGLWLEDLK
REVNNALKNSRIPIITGGSGLYISSLIKGLSSIPQISQEVRENVNELRKNLSKEEFYKLVLSKDPRIQGKIFINDSHRLS
RALEVITETGKTIFVWQENRQPPLFNNFKVYTILPKREDIYRKINSRFIEMVENGAIDEVKNLLSMNPSPHLPAMKAHGV
PEIIRYLKGEITLDEAIQIAQTNTRHYAKRQYTWFKNQFPNSQVIDCANKLTEFGIF

Sequences:

>Translated_297_residues
MKDNIVIITGITASGKSELCDNLIKKHKNISIINCDSKQVYKEIPVITAQPPKQKEFYRLYGYVSARENYSVGLWLEDLK
REVNNALKNSRIPIITGGSGLYISSLIKGLSSIPQISQEVRENVNELRKNLSKEEFYKLVLSKDPRIQGKIFINDSHRLS
RALEVITETGKTIFVWQENRQPPLFNNFKVYTILPKREDIYRKINSRFIEMVENGAIDEVKNLLSMNPSPHLPAMKAHGV
PEIIRYLKGEITLDEAIQIAQTNTRHYAKRQYTWFKNQFPNSQVIDCANKLTEFGIF
>Mature_297_residues
MKDNIVIITGITASGKSELCDNLIKKHKNISIINCDSKQVYKEIPVITAQPPKQKEFYRLYGYVSARENYSVGLWLEDLK
REVNNALKNSRIPIITGGSGLYISSLIKGLSSIPQISQEVRENVNELRKNLSKEEFYKLVLSKDPRIQGKIFINDSHRLS
RALEVITETGKTIFVWQENRQPPLFNNFKVYTILPKREDIYRKINSRFIEMVENGAIDEVKNLLSMNPSPHLPAMKAHGV
PEIIRYLKGEITLDEAIQIAQTNTRHYAKRQYTWFKNQFPNSQVIDCANKLTEFGIF

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=23.2258064516129, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=31.0469314079422, Blast_Score=135, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=26.3843648208469, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_WOLPP (B3CNB0)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001974955.1
- EnsemblBacteria:   EBWOLT00000000981
- GeneID:   6385306
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0135
- GeneTree:   EBGT00050000031197
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK749553
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34219; Mature: 34219

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNIVIITGITASGKSELCDNLIKKHKNISIINCDSKQVYKEIPVITAQPPKQKEFYRL
CCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHCCEEECCCCCHHHHHHH
YGYVSARENYSVGLWLEDLKREVNNALKNSRIPIITGGSGLYISSLIKGLSSIPQISQEV
HHEEECCCCCEEEEEHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
RENVNELRKNLSKEEFYKLVLSKDPRIQGKIFINDSHRLSRALEVITETGKTIFVWQENR
HHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
QPPLFNNFKVYTILPKREDIYRKINSRFIEMVENGAIDEVKNLLSMNPSPHLPAMKAHGV
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHCCH
PEIIRYLKGEITLDEAIQIAQTNTRHYAKRQYTWFKNQFPNSQVIDCANKLTEFGIF
HHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDNIVIITGITASGKSELCDNLIKKHKNISIINCDSKQVYKEIPVITAQPPKQKEFYRL
CCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHCCEEECCCCCHHHHHHH
YGYVSARENYSVGLWLEDLKREVNNALKNSRIPIITGGSGLYISSLIKGLSSIPQISQEV
HHEEECCCCCEEEEEHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
RENVNELRKNLSKEEFYKLVLSKDPRIQGKIFINDSHRLSRALEVITETGKTIFVWQENR
HHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
QPPLFNNFKVYTILPKREDIYRKINSRFIEMVENGAIDEVKNLLSMNPSPHLPAMKAHGV
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHCCH
PEIIRYLKGEITLDEAIQIAQTNTRHYAKRQYTWFKNQFPNSQVIDCANKLTEFGIF
HHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA