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The map label for this gene is hsdM [H]

Identifier: 189499173

GI number: 189499173

Start: 182767

End: 185094

Strand: Reverse

Name: hsdM [H]

Synonym: Cphamn1_0188

Alternate gene names: 189499173

Gene position: 185094-182767 (Counterclockwise)

Preceding gene: 189499174

Following gene: 189499172

Centisome position: 6.76

GC content: 41.07

Gene sequence:

>2328_bases
ATGAATACTGACACAAAAAGACATATTGATTCTGCTCGCCAAGTGTTGGTTGGTGTTGTTCCAAACCCAACTTCACAAAT
CGATCAGATTACCAATGCGCTGATTTATAAATTCATGGACGACATGGATCAAGCGGCTATCAAAGCTGGTGGCTCGCCAT
CTTTTTTTGTTGATGACTTGGAAAATTACGCATGGACACGCTTAATGGATCAACGCATAGGCAATCAAGAGCGTATGAAT
CTTTACAGCGAAGCATTGATCAAGTTTTCTCAAGCCAAGCAGTTGCCGGAGCTTTTCCGTGGTATTTTCAAATCCGCCTT
TTTGCCGTATCGCTCACCCGAAACACTCGGATTGTTTTTGAAAGAAATTGATTATTTTGATTACTCGCATCCTGAAGAAC
TCGGCAATGCATATGAGTATCTTCTTTCTATAATGTCGTCTCAAGGTGATGCCGGACAATTTCGTACCCCGCGTCATATT
ATTGATTTTATAGTGGATGTGGTAAATCCGACCAAGGCTGACAAAGTGCTTGATCCTGCTTGTGGCACAGGCGGATTCTT
GGTCAGCTCATACAAGCACATTTTGGAACAACACGACGGCAAAGACGATCCGAAGAAAAAAGAAAAACCGCTTACGCCTG
ATGAGCGCAAGAAATTGATGACCAATTTTGAGGGCTATGACATAGACCCAACTATGGTGCGTATTGCGCAAGTCAATATG
TATCTGCACCAATTCAAAAATCCAAAAATCTTTCAATACGATTCGCTTTCCAGCGAAGAACGCTGGAATGATAAATTTGA
TGTCATTCTCGCCAATCCTCCGTTTATGTCGCCAAAAGGCGGAATTAAGCCACACAGCAAATTTAGTATTCCATCCAGCC
GTTCTGAAGTGCTGTTTGTGGACTATATAATGAATCATCTCCGGCCAAAAGGTCGTGCCGGTATCATCGTGCCGGAAGGG
ATTATTTTTCAATCAGGCACAGCTCACAAACAGCTTCGCAAGAATCTTGTTGAGGATGGCCTCTATGCCGTGGTTAGTTT
ACCGTCAGGTGTATTTGCGCCATACAGCGGAGTAAAAACCAGTATTTTGCTATTCAATAACGAACTGGCAAAAACAAGTA
CAGAAATTTTGTTTGTAAAAATAGAACAAGACGGCTTTGATCTCGGTGCGACCAAAAGACCAATCAGCAAAAACGATTTA
CCAACGGCGCTGGATATTCTCAACAAATGGAACACAGGCGAAAAAGTAGAAAATAAGCTGGCGGTGTATGTAGAAAAATC
CAAGATTGCCGAAAACGGCGACTATAATCTTTCTGGCGATCGCTATCGCGTAGCGACCGACTACACCAACGCCAAATGGC
CGATGGTGGAGTTGGTGGAGGTGGCGGAGATTTTGAAAGGTTCTGCAATAACTAAAAAAGATACAAAACACGGAAACATT
CCAGTCATTGCTGGCGGACAGGAGCCTGCTTATTATCATAATAAATCAAATAGAGAAGGCGATGTTATCACTGTTAGTGC
TTCAGGTGCATATGCTGGTTTTGTAAATTATTTTACGATTCCAATTTTTGCGTCCGACTGCTCAACCATACAAACAAAAG
ATGAAAACATAGTCAGCACTAGATACCTTTTTAGTATTCTAAAAGCTAAACAAGAAGATATATATGAATTTCAACAAGGT
GGTGGACAGCCGCATGTTTATCCGAAAGACTTAAAAACAATCAAAATCCCTCTCCCACCTCTCGAAATCCAAGAGCAAAT
CGTGGCGGAGCTGGACGGCTACGCAGGCATTATCGCCGGCGCCAAACAAATCGCCCAAAACTGGAAACCCAAAATTGAAA
TTGATCCGGAGTGGGAGAAGGTGAAGTTGGGAGAAATATCTGACAGGGTTACAAAAGGAACTACACCGACTACAAATGGA
TTCCAATTTCAAGAGAGCGGGATTAATTTTATAAAGATTGAATCAATTGATGATGGTGGATATTTTATCAGGGAAAAACT
TGCTCATATAAATCAGGAGTGCAATGAATCACTAAAGAGATCACAATTAAAAGAAAATGATATTTTGTTTTCAATCGCTG
GCGCTCTTGGACGTGTCGCCTCGATAGAATCTTCAATATTACCAGCGAACACTAATCAAGCACTTGCGATAATTTCACCA
AAAAAAGAACTTGATTCAAAATATCTTGAACAAGTTTTGAGATCAGATTTGATTCAGAATCAAATTTTTGGTTTGAAAGT
TGGGGTTGCTCAAAGCAATTTATCATTGGCTCAAGTTTCAGACTTTGAAATCCCTCTCCCATCCCTTGAAATCAAAAACA
AATCGTGA

Upstream 100 bases:

>100_bases
ATCGTTTGATTTTGAAGATTATCAAAAACTTAACGGGTATAAAACTATCGTTCCGCAGTATGTTCGGGATTATGCCTATA
ATTTAACCAATTTATAAAAT

Downstream 100 bases:

>100_bases
AAAAAATCGAAGCCGAGCGTGTGCTTGTGGATGGAAATAAAAAGTTGATTGAAATATACGAACAAAAAACCAAGGATACA
ATCGCCAAACTTTGGAAAGA

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MNTDTKRHIDSARQVLVGVVPNPTSQIDQITNALIYKFMDDMDQAAIKAGGSPSFFVDDLENYAWTRLMDQRIGNQERMN
LYSEALIKFSQAKQLPELFRGIFKSAFLPYRSPETLGLFLKEIDYFDYSHPEELGNAYEYLLSIMSSQGDAGQFRTPRHI
IDFIVDVVNPTKADKVLDPACGTGGFLVSSYKHILEQHDGKDDPKKKEKPLTPDERKKLMTNFEGYDIDPTMVRIAQVNM
YLHQFKNPKIFQYDSLSSEERWNDKFDVILANPPFMSPKGGIKPHSKFSIPSSRSEVLFVDYIMNHLRPKGRAGIIVPEG
IIFQSGTAHKQLRKNLVEDGLYAVVSLPSGVFAPYSGVKTSILLFNNELAKTSTEILFVKIEQDGFDLGATKRPISKNDL
PTALDILNKWNTGEKVENKLAVYVEKSKIAENGDYNLSGDRYRVATDYTNAKWPMVELVEVAEILKGSAITKKDTKHGNI
PVIAGGQEPAYYHNKSNREGDVITVSASGAYAGFVNYFTIPIFASDCSTIQTKDENIVSTRYLFSILKAKQEDIYEFQQG
GGQPHVYPKDLKTIKIPLPPLEIQEQIVAELDGYAGIIAGAKQIAQNWKPKIEIDPEWEKVKLGEISDRVTKGTTPTTNG
FQFQESGINFIKIESIDDGGYFIREKLAHINQECNESLKRSQLKENDILFSIAGALGRVASIESSILPANTNQALAIISP
KKELDSKYLEQVLRSDLIQNQIFGLKVGVAQSNLSLAQVSDFEIPLPSLEIKNKS

Sequences:

>Translated_775_residues
MNTDTKRHIDSARQVLVGVVPNPTSQIDQITNALIYKFMDDMDQAAIKAGGSPSFFVDDLENYAWTRLMDQRIGNQERMN
LYSEALIKFSQAKQLPELFRGIFKSAFLPYRSPETLGLFLKEIDYFDYSHPEELGNAYEYLLSIMSSQGDAGQFRTPRHI
IDFIVDVVNPTKADKVLDPACGTGGFLVSSYKHILEQHDGKDDPKKKEKPLTPDERKKLMTNFEGYDIDPTMVRIAQVNM
YLHQFKNPKIFQYDSLSSEERWNDKFDVILANPPFMSPKGGIKPHSKFSIPSSRSEVLFVDYIMNHLRPKGRAGIIVPEG
IIFQSGTAHKQLRKNLVEDGLYAVVSLPSGVFAPYSGVKTSILLFNNELAKTSTEILFVKIEQDGFDLGATKRPISKNDL
PTALDILNKWNTGEKVENKLAVYVEKSKIAENGDYNLSGDRYRVATDYTNAKWPMVELVEVAEILKGSAITKKDTKHGNI
PVIAGGQEPAYYHNKSNREGDVITVSASGAYAGFVNYFTIPIFASDCSTIQTKDENIVSTRYLFSILKAKQEDIYEFQQG
GGQPHVYPKDLKTIKIPLPPLEIQEQIVAELDGYAGIIAGAKQIAQNWKPKIEIDPEWEKVKLGEISDRVTKGTTPTTNG
FQFQESGINFIKIESIDDGGYFIREKLAHINQECNESLKRSQLKENDILFSIAGALGRVASIESSILPANTNQALAIISP
KKELDSKYLEQVLRSDLIQNQIFGLKVGVAQSNLSLAQVSDFEIPLPSLEIKNKS
>Mature_775_residues
MNTDTKRHIDSARQVLVGVVPNPTSQIDQITNALIYKFMDDMDQAAIKAGGSPSFFVDDLENYAWTRLMDQRIGNQERMN
LYSEALIKFSQAKQLPELFRGIFKSAFLPYRSPETLGLFLKEIDYFDYSHPEELGNAYEYLLSIMSSQGDAGQFRTPRHI
IDFIVDVVNPTKADKVLDPACGTGGFLVSSYKHILEQHDGKDDPKKKEKPLTPDERKKLMTNFEGYDIDPTMVRIAQVNM
YLHQFKNPKIFQYDSLSSEERWNDKFDVILANPPFMSPKGGIKPHSKFSIPSSRSEVLFVDYIMNHLRPKGRAGIIVPEG
IIFQSGTAHKQLRKNLVEDGLYAVVSLPSGVFAPYSGVKTSILLFNNELAKTSTEILFVKIEQDGFDLGATKRPISKNDL
PTALDILNKWNTGEKVENKLAVYVEKSKIAENGDYNLSGDRYRVATDYTNAKWPMVELVEVAEILKGSAITKKDTKHGNI
PVIAGGQEPAYYHNKSNREGDVITVSASGAYAGFVNYFTIPIFASDCSTIQTKDENIVSTRYLFSILKAKQEDIYEFQQG
GGQPHVYPKDLKTIKIPLPPLEIQEQIVAELDGYAGIIAGAKQIAQNWKPKIEIDPEWEKVKLGEISDRVTKGTTPTTNG
FQFQESGINFIKIESIDDGGYFIREKLAHINQECNESLKRSQLKENDILFSIAGALGRVASIESSILPANTNQALAIISP
KKELDSKYLEQVLRSDLIQNQIFGLKVGVAQSNLSLAQVSDFEIPLPSLEIKNKS

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=319, Percent_Identity=27.5862068965517, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 86800; Mature: 86800

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDTKRHIDSARQVLVGVVPNPTSQIDQITNALIYKFMDDMDQAAIKAGGSPSFFVDDL
CCCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHH
ENYAWTRLMDQRIGNQERMNLYSEALIKFSQAKQLPELFRGIFKSAFLPYRSPETLGLFL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KEIDYFDYSHPEELGNAYEYLLSIMSSQGDAGQFRTPRHIIDFIVDVVNPTKADKVLDPA
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCC
CGTGGFLVSSYKHILEQHDGKDDPKKKEKPLTPDERKKLMTNFEGYDIDPTMVRIAQVNM
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YLHQFKNPKIFQYDSLSSEERWNDKFDVILANPPFMSPKGGIKPHSKFSIPSSRSEVLFV
HHHHCCCCCEEEECCCCCHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DYIMNHLRPKGRAGIIVPEGIIFQSGTAHKQLRKNLVEDGLYAVVSLPSGVFAPYSGVKT
HHHHHHCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
SILLFNNELAKTSTEILFVKIEQDGFDLGATKRPISKNDLPTALDILNKWNTGEKVENKL
EEEEECCHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCCE
AVYVEKSKIAENGDYNLSGDRYRVATDYTNAKWPMVELVEVAEILKGSAITKKDTKHGNI
EEEEEHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
PVIAGGQEPAYYHNKSNREGDVITVSASGAYAGFVNYFTIPIFASDCSTIQTKDENIVST
CEEECCCCCCEECCCCCCCCCEEEEECCCCHHHHHHEEEEEEEECCCCCCCCCCCCHHHH
RYLFSILKAKQEDIYEFQQGGGQPHVYPKDLKTIKIPLPPLEIQEQIVAELDGYAGIIAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHH
AKQIAQNWKPKIEIDPEWEKVKLGEISDRVTKGTTPTTNGFQFQESGINFIKIESIDDGG
HHHHHHCCCCCEEECCCCCEEECCCHHHHHHCCCCCCCCCEEECCCCCCEEEEEEECCCC
YFIREKLAHINQECNESLKRSQLKENDILFSIAGALGRVASIESSILPANTNQALAIISP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEECC
KKELDSKYLEQVLRSDLIQNQIFGLKVGVAQSNLSLAQVSDFEIPLPSLEIKNKS
HHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTDTKRHIDSARQVLVGVVPNPTSQIDQITNALIYKFMDDMDQAAIKAGGSPSFFVDDL
CCCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHH
ENYAWTRLMDQRIGNQERMNLYSEALIKFSQAKQLPELFRGIFKSAFLPYRSPETLGLFL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KEIDYFDYSHPEELGNAYEYLLSIMSSQGDAGQFRTPRHIIDFIVDVVNPTKADKVLDPA
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCC
CGTGGFLVSSYKHILEQHDGKDDPKKKEKPLTPDERKKLMTNFEGYDIDPTMVRIAQVNM
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YLHQFKNPKIFQYDSLSSEERWNDKFDVILANPPFMSPKGGIKPHSKFSIPSSRSEVLFV
HHHHCCCCCEEEECCCCCHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DYIMNHLRPKGRAGIIVPEGIIFQSGTAHKQLRKNLVEDGLYAVVSLPSGVFAPYSGVKT
HHHHHHCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
SILLFNNELAKTSTEILFVKIEQDGFDLGATKRPISKNDLPTALDILNKWNTGEKVENKL
EEEEECCHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCCE
AVYVEKSKIAENGDYNLSGDRYRVATDYTNAKWPMVELVEVAEILKGSAITKKDTKHGNI
EEEEEHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
PVIAGGQEPAYYHNKSNREGDVITVSASGAYAGFVNYFTIPIFASDCSTIQTKDENIVST
CEEECCCCCCEECCCCCCCCCEEEEECCCCHHHHHHEEEEEEEECCCCCCCCCCCCHHHH
RYLFSILKAKQEDIYEFQQGGGQPHVYPKDLKTIKIPLPPLEIQEQIVAELDGYAGIIAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHH
AKQIAQNWKPKIEIDPEWEKVKLGEISDRVTKGTTPTTNGFQFQESGINFIKIESIDDGG
HHHHHHCCCCCEEECCCCCEEECCCHHHHHHCCCCCCCCCEEECCCCCCEEEEEEECCCC
YFIREKLAHINQECNESLKRSQLKENDILFSIAGALGRVASIESSILPANTNQALAIISP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEECC
KKELDSKYLEQVLRSDLIQNQIFGLKVGVAQSNLSLAQVSDFEIPLPSLEIKNKS
HHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]