Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 189499154

GI number: 189499154

Start: 163100

End: 164128

Strand: Reverse

Name: pheS

Synonym: Cphamn1_0168

Alternate gene names: 189499154

Gene position: 164128-163100 (Counterclockwise)

Preceding gene: 189499155

Following gene: 189499139

Centisome position: 6.0

GC content: 48.79

Gene sequence:

>1029_bases
ATGAAGAACCGCATAGAAGCAATAAAACAGCAGATTGATGCGTTCGAGCTCAACAGTGCTGATAAACTGGAGGCTTTCCG
TCTCCAGTTTTTGGTTCGTAAAGGAAGTATTGCGGAGCTGTTCAATCAGCTCAAAGAACTTTCCCCTGTTGACCGACCGG
AATACGGGCAACGGCTGAATAAGCTGAAAAAGTTTGCAGAAAGCACCTATGAAGAGGCTGAAAAAAAGCTCGATGTACTC
GCTTCCGGCAAAGGCAAACAGCATAGAGATCTGACGCTTCCCGGCAGACGACAGTATACCGGTTCGGAACATCCTGTACA
GCAGGTACTCGGAGATATGAAACAGATCTTCAAGGCGATGGGCTTTACCATAGCAACCGGTCCTGAACTGGAACTCGACA
GCTATAACTTCGACAAGCTCAACTTTCCGTCCAACCACCCGGCACGGGACATGCAGGATACTTTTTTCATCACGAAAAAC
GGGAAGGATGACGACCTCCTTTTACGCACACATACATCACCTGTGCAGATCAGGGTTATGCTTGACGAAAAACCTCCGAT
ACGGGTCATCTGTCCGGGAAAGGTATACCGTAATGAGGCAATCAGTGCCCGAAGCTACTGTGTATTCCATCAGCTGGAAG
GGCTCTATATCGATAAGCGGGTCTCCTTTGCAGACCTGAAGGCCACAATCTATTCATTTGCCAAACAGATGTTCGGCAGT
GACGTACAATTGCGTTTCAGACCAAGTTTTTTCCCGTTTACCGAACCATCTGCCGAGGTGGACGTCACCTGTTACCTCTG
CGGAGGAAAAGGGTGCCGGGTCTGCAAACAATCCGGATGGCTCGAAATCCTCGGGTGCGGAATGGTACACCCGAACGTGC
TCAGAAACTGCTCCATAGATCCTGACGAATATTCAGGATTTGCCTTTGGCATGGGCGTTGACCGGACAGCCCTCTTACGC
TACAAAATCGACGACATTCGTCTTCTTTTTGAAAACGACGTAAGAATGCTCGAACAGTTTTCCAGGTAA

Upstream 100 bases:

>100_bases
ACCGGAAAGCTCTTGCTGAAATAGCGGTTAAAGATCCAGCCGCTTTCAGCCAGATCGTTAAATCCGCTATGGGCTGAACA
TTCAGGAGTGATCCAGATTC

Downstream 100 bases:

>100_bases
ACAGTCAGACTACCGTTTTTCCAGCTCCCACCACCCTTTCTGTTTCCGGAAAAAACAGTACAAGGGTGGGATGCCTACCG
GACGGTAGCTACCTGTCCCT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKNRIEAIKQQIDAFELNSADKLEAFRLQFLVRKGSIAELFNQLKELSPVDRPEYGQRLNKLKKFAESTYEEAEKKLDVL
ASGKGKQHRDLTLPGRRQYTGSEHPVQQVLGDMKQIFKAMGFTIATGPELELDSYNFDKLNFPSNHPARDMQDTFFITKN
GKDDDLLLRTHTSPVQIRVMLDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKRVSFADLKATIYSFAKQMFGS
DVQLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKQSGWLEILGCGMVHPNVLRNCSIDPDEYSGFAFGMGVDRTALLR
YKIDDIRLLFENDVRMLEQFSR

Sequences:

>Translated_342_residues
MKNRIEAIKQQIDAFELNSADKLEAFRLQFLVRKGSIAELFNQLKELSPVDRPEYGQRLNKLKKFAESTYEEAEKKLDVL
ASGKGKQHRDLTLPGRRQYTGSEHPVQQVLGDMKQIFKAMGFTIATGPELELDSYNFDKLNFPSNHPARDMQDTFFITKN
GKDDDLLLRTHTSPVQIRVMLDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKRVSFADLKATIYSFAKQMFGS
DVQLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKQSGWLEILGCGMVHPNVLRNCSIDPDEYSGFAFGMGVDRTALLR
YKIDDIRLLFENDVRMLEQFSR
>Mature_342_residues
MKNRIEAIKQQIDAFELNSADKLEAFRLQFLVRKGSIAELFNQLKELSPVDRPEYGQRLNKLKKFAESTYEEAEKKLDVL
ASGKGKQHRDLTLPGRRQYTGSEHPVQQVLGDMKQIFKAMGFTIATGPELELDSYNFDKLNFPSNHPARDMQDTFFITKN
GKDDDLLLRTHTSPVQIRVMLDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKRVSFADLKATIYSFAKQMFGS
DVQLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKQSGWLEILGCGMVHPNVLRNCSIDPDEYSGFAFGMGVDRTALLR
YKIDDIRLLFENDVRMLEQFSR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=277, Percent_Identity=30.3249097472924, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI5729820, Length=294, Percent_Identity=27.2108843537415, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1788007, Length=320, Percent_Identity=46.875, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI32563657, Length=309, Percent_Identity=28.4789644012945, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=309, Percent_Identity=28.4789644012945, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=262, Percent_Identity=28.6259541984733, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321087, Length=359, Percent_Identity=27.0194986072423, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=102, Percent_Identity=38.2352941176471, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI18858079, Length=381, Percent_Identity=26.246719160105, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=25.5905511811024, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CHLPB (B3EKG5)

Other databases:

- EMBL:   CP001101
- RefSeq:   YP_001958624.1
- ProteinModelPortal:   B3EKG5
- SMR:   B3EKG5
- GeneID:   6373822
- GenomeReviews:   CP001101_GR
- KEGG:   cpb:Cphamn1_0168
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39186; Mature: 39186

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRIEAIKQQIDAFELNSADKLEAFRLQFLVRKGSIAELFNQLKELSPVDRPEYGQRLN
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHH
KLKKFAESTYEEAEKKLDVLASGKGKQHRDLTLPGRRQYTGSEHPVQQVLGDMKQIFKAM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GFTIATGPELELDSYNFDKLNFPSNHPARDMQDTFFITKNGKDDDLLLRTHTSPVQIRVM
CCEEECCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEEEE
LDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKRVSFADLKATIYSFAKQMFGS
ECCCCCEEEECCCHHHHHHHHCHHHEEEEEHHCCEEEECCCCHHHHHHHHHHHHHHHCCC
DVQLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKQSGWLEILGCGMVHPNVLRNCSID
CEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCEEEEECCCCCHHHHHCCCCC
PDEYSGFAFGMGVDRTALLRYKIDDIRLLFENDVRMLEQFSR
CCCCCCEEEECCCCCEEEEEEEHHHHEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKNRIEAIKQQIDAFELNSADKLEAFRLQFLVRKGSIAELFNQLKELSPVDRPEYGQRLN
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHH
KLKKFAESTYEEAEKKLDVLASGKGKQHRDLTLPGRRQYTGSEHPVQQVLGDMKQIFKAM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GFTIATGPELELDSYNFDKLNFPSNHPARDMQDTFFITKNGKDDDLLLRTHTSPVQIRVM
CCEEECCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEEEE
LDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKRVSFADLKATIYSFAKQMFGS
ECCCCCEEEECCCHHHHHHHHCHHHEEEEEHHCCEEEECCCCHHHHHHHHHHHHHHHCCC
DVQLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKQSGWLEILGCGMVHPNVLRNCSID
CEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCEEEEECCCCCHHHHHCCCCC
PDEYSGFAFGMGVDRTALLRYKIDDIRLLFENDVRMLEQFSR
CCCCCCEEEECCCCCEEEEEEEHHHHEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA