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The map label for this gene is mrpA [H]

Identifier: 189499068

GI number: 189499068

Start: 70536

End: 72833

Strand: Direct

Name: mrpA [H]

Synonym: Cphamn1_0074

Alternate gene names: 189499068

Gene position: 70536-72833 (Clockwise)

Preceding gene: 189499067

Following gene: 189499069

Centisome position: 2.58

GC content: 51.96

Gene sequence:

>2298_bases
ATGCTGATTCTGTTAGTTATAGGATTTCTTGCCTCGGCGATCGCGCCGTTGCTGTATAAACGGTTCAGGGAGAATTTCGG
CTGGATTGCCGTGGTGTTTCCCCTGTTCATGTTTTTCGGCTTTTTGTCGAAATATCCGACGATTGCCGCCGGCGAAGTTC
TCAGGGAGTCGGTCCCGTGGGTGCCTTCTCTGGGGATCAACTTTTCTTTTGTTCTCGACGGCCTGAGCATGACTTTTGCG
CTGATCATCACCCTGATCGGTGCAGCGGTGTTTCTCTATGCGGGAGCCTATATGAAGGCGTATGTGCAAACCGGCAGGTT
TTATGTGTATATCGGCATCTTCATGACCTCGATGCTGGGACTCGTGCTGTCGGACAACATGCTCCTGATGTTTGTATTCT
GGGAACTGACAAGCATCAGCTCCTTTCTCCTGATAGGCTTCACGCATCATAAGGAGGCGTCAAGGAAATCGGCATTGCAG
GCTCTCCTTGTTACGGGTGGCGGGGGACTTGCGCTGCTTGTGGGGATCATAATGCTCTATCGGGTGACGGGGAGTTTCGA
GATCTCTTCGTTTTATGATATGAACGGCGTGATTACCGCTCATAGTCTTTATCCCGCTATCGTTGTTCTGCTCCTGATCG
GCGCGTTTACCAAGTCGGCGCAGTTCCCTTTCCATTTCTGGCTTCCCAACGCCATGGAGGCGCCCACTCCTGTCAGCGCC
TACCTGCATTCGGCAACGATGGTCAAGGCCGGCATTTACCTGATAGCAAGAATGAACCACGAGATCGGCGGCACGCCGGT
CTGGCAGGATACGGTACTGATAGCGGGGGCATCGACCATGCTCTTTACTGCTCTGCTCTCCTTCAGGCAGACCGACCTGA
AAAAATTGCTTGCCTACTCTACCCTGTCTGTGCTGGGAACTCTCGTCATGCTGCTCGGCATTGGTTCGGAGCTAGCCATC
AAGGCGTTTTTTATCTACATCATCGCGCATTCGCTCTACAAGGGCACGCTGTTTCTTGTCGCGGGCACACTCGACCATGC
GACAGGAACACGAGACGTGACGAAACTTGGGGGGTTACGCAAACTGATGCCGGTGACAGCCTTTACGGCAGGGTTGGCGT
CGTTTTCCATGATGGGCGTGATTCCTCTGGTCGGGTTTATCGGTAAGGAAACTGTCTATAAAGCTATTCTCGGACTGGAG
TACTGGGGAGGTTTTCTCATCGTGGCAGCTTTGTTTGCCAATGCTTTTGTTGTGATGATAACGCTGCTTGTTGGATTCAA
GCCCTTCTGGGGGGCCATGCTTCCGACGCCGAAGCAGCCGGAAGAGGCTCCGCTCAAGATGCTTATCGGTCCTGCCCTGC
TTGCGGTTCTGGGATTGCTTTTCGGGCTTTTCCCCGACTTTTTTATCGCCACTATGCTCGAACAGTCGGCACAGAGCATT
TTGTCTGAAAAGCTTAGCCTGAAAATCTCGATATGGGAAGGTTTCAACCTTGTGCTGCTGCTAAGCTTTGTAACGCTTTT
GCTTGGCGGACTCGTCTATCTGATTCGGGGAAGGGTGGCTGACAGGATGAAGCATGTTTCGTTGCCGGCTGTCATCAAGC
CCTCTGCGTGGTATGATGGTGCTATGGCTGGACTGTTGTCGTTTGCGACGCTGCAGACCCGGATTGTGCAGAATGGATAC
CTGCGCAACTATATCATCGTCATTCTGGTAACTGCTCTATCTCTGGCCTCATTTGCTTTTTATCAGGCGGTTGAAGAATT
AATCATTACCCCTGACTTTTCGATTACCTTTTACGAAGGTGCACTGGCGTTCATTATTCTTGTTTCAACGTTTCTTCTGA
TTACCTCCAGGTCAAGACTGAAATCGATCGTTGCGCTCGGCGCACTGGGTTTTTCCATCGGTATCATTTTCGTGATTTAC
GGTGCGCCCGATCTGGCTCTGACGACATTCGCGATTGAGACGCTCAATGTAATCCTGTTCGTGCTGGTGCTCTACAGGCT
CCCCCGTTTCCTGAAACTTTCCCGCTCGTCCAACAGGGTGAGAGATTCTCTGATAGCAACGTGCGTCGGAGTGTTCATGA
CCCTTACGGTACTTTTTGCCACGTCGTTCGAACTTTCGTCCGAACTGAAGCGATATTTTGCTGAAGCCAGTGTTCCTTAC
GGCAAGGGCCATAATGTGGTCAATGTCATCCTTGTTGATTTCAGGGCGATCGACACGCTGGGGGAGATCACGGTGCTTGC
GGTTGCCGCGCTTGGCGTCTTCGCCCTGCTCAAATTGAAACCGCAAGGGAGGGAGTGA

Upstream 100 bases:

>100_bases
GGTTCCTGAAGCTGACGCATTTGCCCCTCTTCAGGGAACAAACGGGAGCATCCGGGGGTTATTTCAGGCTGATCCGCTTC
TATAATGTGAGTGTGAAAAC

Downstream 100 bases:

>100_bases
TCATGTATTCCGTCATACTTGCCACCGCATCCCGTTATCTGCTGATTCTACTGCTGATGTTCTCGGTGTTTCTTCTGCTG
CGCGGGCATAACGAACCGGG

Product: putative monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MLILLVIGFLASAIAPLLYKRFRENFGWIAVVFPLFMFFGFLSKYPTIAAGEVLRESVPWVPSLGINFSFVLDGLSMTFA
LIITLIGAAVFLYAGAYMKAYVQTGRFYVYIGIFMTSMLGLVLSDNMLLMFVFWELTSISSFLLIGFTHHKEASRKSALQ
ALLVTGGGGLALLVGIIMLYRVTGSFEISSFYDMNGVITAHSLYPAIVVLLLIGAFTKSAQFPFHFWLPNAMEAPTPVSA
YLHSATMVKAGIYLIARMNHEIGGTPVWQDTVLIAGASTMLFTALLSFRQTDLKKLLAYSTLSVLGTLVMLLGIGSELAI
KAFFIYIIAHSLYKGTLFLVAGTLDHATGTRDVTKLGGLRKLMPVTAFTAGLASFSMMGVIPLVGFIGKETVYKAILGLE
YWGGFLIVAALFANAFVVMITLLVGFKPFWGAMLPTPKQPEEAPLKMLIGPALLAVLGLLFGLFPDFFIATMLEQSAQSI
LSEKLSLKISIWEGFNLVLLLSFVTLLLGGLVYLIRGRVADRMKHVSLPAVIKPSAWYDGAMAGLLSFATLQTRIVQNGY
LRNYIIVILVTALSLASFAFYQAVEELIITPDFSITFYEGALAFIILVSTFLLITSRSRLKSIVALGALGFSIGIIFVIY
GAPDLALTTFAIETLNVILFVLVLYRLPRFLKLSRSSNRVRDSLIATCVGVFMTLTVLFATSFELSSELKRYFAEASVPY
GKGHNVVNVILVDFRAIDTLGEITVLAVAALGVFALLKLKPQGRE

Sequences:

>Translated_765_residues
MLILLVIGFLASAIAPLLYKRFRENFGWIAVVFPLFMFFGFLSKYPTIAAGEVLRESVPWVPSLGINFSFVLDGLSMTFA
LIITLIGAAVFLYAGAYMKAYVQTGRFYVYIGIFMTSMLGLVLSDNMLLMFVFWELTSISSFLLIGFTHHKEASRKSALQ
ALLVTGGGGLALLVGIIMLYRVTGSFEISSFYDMNGVITAHSLYPAIVVLLLIGAFTKSAQFPFHFWLPNAMEAPTPVSA
YLHSATMVKAGIYLIARMNHEIGGTPVWQDTVLIAGASTMLFTALLSFRQTDLKKLLAYSTLSVLGTLVMLLGIGSELAI
KAFFIYIIAHSLYKGTLFLVAGTLDHATGTRDVTKLGGLRKLMPVTAFTAGLASFSMMGVIPLVGFIGKETVYKAILGLE
YWGGFLIVAALFANAFVVMITLLVGFKPFWGAMLPTPKQPEEAPLKMLIGPALLAVLGLLFGLFPDFFIATMLEQSAQSI
LSEKLSLKISIWEGFNLVLLLSFVTLLLGGLVYLIRGRVADRMKHVSLPAVIKPSAWYDGAMAGLLSFATLQTRIVQNGY
LRNYIIVILVTALSLASFAFYQAVEELIITPDFSITFYEGALAFIILVSTFLLITSRSRLKSIVALGALGFSIGIIFVIY
GAPDLALTTFAIETLNVILFVLVLYRLPRFLKLSRSSNRVRDSLIATCVGVFMTLTVLFATSFELSSELKRYFAEASVPY
GKGHNVVNVILVDFRAIDTLGEITVLAVAALGVFALLKLKPQGRE
>Mature_765_residues
MLILLVIGFLASAIAPLLYKRFRENFGWIAVVFPLFMFFGFLSKYPTIAAGEVLRESVPWVPSLGINFSFVLDGLSMTFA
LIITLIGAAVFLYAGAYMKAYVQTGRFYVYIGIFMTSMLGLVLSDNMLLMFVFWELTSISSFLLIGFTHHKEASRKSALQ
ALLVTGGGGLALLVGIIMLYRVTGSFEISSFYDMNGVITAHSLYPAIVVLLLIGAFTKSAQFPFHFWLPNAMEAPTPVSA
YLHSATMVKAGIYLIARMNHEIGGTPVWQDTVLIAGASTMLFTALLSFRQTDLKKLLAYSTLSVLGTLVMLLGIGSELAI
KAFFIYIIAHSLYKGTLFLVAGTLDHATGTRDVTKLGGLRKLMPVTAFTAGLASFSMMGVIPLVGFIGKETVYKAILGLE
YWGGFLIVAALFANAFVVMITLLVGFKPFWGAMLPTPKQPEEAPLKMLIGPALLAVLGLLFGLFPDFFIATMLEQSAQSI
LSEKLSLKISIWEGFNLVLLLSFVTLLLGGLVYLIRGRVADRMKHVSLPAVIKPSAWYDGAMAGLLSFATLQTRIVQNGY
LRNYIIVILVTALSLASFAFYQAVEELIITPDFSITFYEGALAFIILVSTFLLITSRSRLKSIVALGALGFSIGIIFVIY
GAPDLALTTFAIETLNVILFVLVLYRLPRFLKLSRSSNRVRDSLIATCVGVFMTLTVLFATSFELSSELKRYFAEASVPY
GKGHNVVNVILVDFRAIDTLGEITVLAVAALGVFALLKLKPQGRE

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=345, Percent_Identity=33.6231884057971, Blast_Score=177, Evalue=4e-44,
Organism=Homo sapiens, GI251831116, Length=300, Percent_Identity=26.3333333333333, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1788614, Length=358, Percent_Identity=37.1508379888268, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788829, Length=347, Percent_Identity=35.1585014409222, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1788827, Length=423, Percent_Identity=26.241134751773, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788831, Length=339, Percent_Identity=31.5634218289086, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1788613, Length=347, Percent_Identity=27.9538904899135, Blast_Score=120, Evalue=5e-28,
Organism=Escherichia coli, GI2367154, Length=480, Percent_Identity=26.4583333333333, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693160, Length=384, Percent_Identity=23.1770833333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 83554; Mature: 83554

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILLVIGFLASAIAPLLYKRFRENFGWIAVVFPLFMFFGFLSKYPTIAAGEVLRESVPW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
VPSLGINFSFVLDGLSMTFALIITLIGAAVFLYAGAYMKAYVQTGRFYVYIGIFMTSMLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
LVLSDNMLLMFVFWELTSISSFLLIGFTHHKEASRKSALQALLVTGGGGLALLVGIIMLY
HHHCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHH
RVTGSFEISSFYDMNGVITAHSLYPAIVVLLLIGAFTKSAQFPFHFWLPNAMEAPTPVSA
HHCCCEEECCEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHH
YLHSATMVKAGIYLIARMNHEIGGTPVWQDTVLIAGASTMLFTALLSFRQTDLKKLLAYS
HHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
TLSVLGTLVMLLGIGSELAIKAFFIYIIAHSLYKGTLFLVAGTLDHATGTRDVTKLGGLR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHCCHH
KLMPVTAFTAGLASFSMMGVIPLVGFIGKETVYKAILGLEYWGGFLIVAALFANAFVVMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLLVGFKPFWGAMLPTPKQPEEAPLKMLIGPALLAVLGLLFGLFPDFFIATMLEQSAQSI
HHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSEKLSLKISIWEGFNLVLLLSFVTLLLGGLVYLIRGRVADRMKHVSLPAVIKPSAWYDG
HHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHH
AMAGLLSFATLQTRIVQNGYLRNYIIVILVTALSLASFAFYQAVEELIITPDFSITFYEG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHH
ALAFIILVSTFLLITSRSRLKSIVALGALGFSIGIIFVIYGAPDLALTTFAIETLNVILF
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHH
VLVLYRLPRFLKLSRSSNRVRDSLIATCVGVFMTLTVLFATSFELSSELKRYFAEASVPY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GKGHNVVNVILVDFRAIDTLGEITVLAVAALGVFALLKLKPQGRE
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLILLVIGFLASAIAPLLYKRFRENFGWIAVVFPLFMFFGFLSKYPTIAAGEVLRESVPW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
VPSLGINFSFVLDGLSMTFALIITLIGAAVFLYAGAYMKAYVQTGRFYVYIGIFMTSMLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
LVLSDNMLLMFVFWELTSISSFLLIGFTHHKEASRKSALQALLVTGGGGLALLVGIIMLY
HHHCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHH
RVTGSFEISSFYDMNGVITAHSLYPAIVVLLLIGAFTKSAQFPFHFWLPNAMEAPTPVSA
HHCCCEEECCEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHH
YLHSATMVKAGIYLIARMNHEIGGTPVWQDTVLIAGASTMLFTALLSFRQTDLKKLLAYS
HHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
TLSVLGTLVMLLGIGSELAIKAFFIYIIAHSLYKGTLFLVAGTLDHATGTRDVTKLGGLR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHCCHH
KLMPVTAFTAGLASFSMMGVIPLVGFIGKETVYKAILGLEYWGGFLIVAALFANAFVVMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLLVGFKPFWGAMLPTPKQPEEAPLKMLIGPALLAVLGLLFGLFPDFFIATMLEQSAQSI
HHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSEKLSLKISIWEGFNLVLLLSFVTLLLGGLVYLIRGRVADRMKHVSLPAVIKPSAWYDG
HHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHH
AMAGLLSFATLQTRIVQNGYLRNYIIVILVTALSLASFAFYQAVEELIITPDFSITFYEG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHH
ALAFIILVSTFLLITSRSRLKSIVALGALGFSIGIIFVIYGAPDLALTTFAIETLNVILF
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHH
VLVLYRLPRFLKLSRSSNRVRDSLIATCVGVFMTLTVLFATSFELSSELKRYFAEASVPY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GKGHNVVNVILVDFRAIDTLGEITVLAVAALGVFALLKLKPQGRE
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]