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The map label for this gene is mjaIM [H]

Identifier: 189499041

GI number: 189499041

Start: 44542

End: 45867

Strand: Direct

Name: mjaIM [H]

Synonym: Cphamn1_0041

Alternate gene names: 189499041

Gene position: 44542-45867 (Clockwise)

Preceding gene: 189499040

Following gene: 189499042

Centisome position: 1.63

GC content: 46.3

Gene sequence:

>1326_bases
ATGTCTATTACTAAATGCTTTAAGAAGAGCGTATTGACAACCCATCATACCATCATACAAGGCGACAGCCGACAGATGAA
CCTGCTGTCCGACAGGTCGATTCATCTTGTTATCACCTCTCCCCCCTACTGGCAGCTAAAGGATTACGGTACGGAAAGTC
AGATCGGCTTTCATGAGAGCTATGAAAGCTATATCAATAATCTGAATCTTGTCTGGAGCGAATGTGAAAGGGCGCTGTAT
CCGGGTTGCCGACTTTGTGTGAATATCGGCGACCAGTTCGCCCGTTCGGTCTACTACGGCAGGTACAAGGTTATTCCCAT
CAGGACGGAGATCATCAGGTTCTGCGAGACTATCGGCTTTGATTATATGGGTGCGGTGATCTGGCAAAAGGTAACGACAA
CCAATACCACAGGTGGGGCATCGATCATGGGCAGCTTTTCCTACCCCCGCAATGGTATTCTGAAGCTGGATTATGAGTTC
ATTCTCATCTTCAAAAAGCTGGGGGACGCGCCAAAGCCGACAAAAGAACAGAAAGAGCGTTCCGCAATGACCAAGGAGGA
ATGGAATACTTATTTCTCGGGCCATTGGAATTTCGCCGGTGCAAAGCAGGACGGACACATAGCCATGTTTCCCGAAGAGT
TGCCAAGACGTCTTATCAAGATGTTTGCTTTTGAGGGCGATACAGTGCTCGATCCGTTTATGGGAAGCGGGACAACCAAT
CTTGCGGCGAGGAACCTCGGGCGAAACTCGGTTGGCTACGAGATCAATTCCGAGTTTGTCGAAATATCAAAACAGAAACT
CGGTGTCAACCAGCCGGATCTTGTGGGTACCAAGTATGACTTTCAATACGATACAGTATCAGGTGATTTCGAGATAGCTA
TCCAGCAACTTCCTTATCGGTTCCAGGATCCTCACAAGCTGGATAAGAAAGTAGATCCAAGAAAGCTCTCCTTCGGTTCA
AGAATAGACAAGGAAAACGGCGCACAACGCGAAGAGCTGTTTACAGTCAAAGAGGCACTGAGTCCCGAAAAGCTGCTGCT
GTCAAATGGATTGACGATCAGGCTGCTTGGGATCAAATCCGATCCTGTACTAAAAGATAAGGCTGTCGGGTATCTGAATG
AAAAGATTAAAGGTAAGCGGATATTTCTTAAGTACGACCGGAAGAAATACGATGAGGAGAACAATCTTCTGTGCTACCTC
TATCTGCAAAACAAGACATTCGTCAACGCTCATTTGATTCGTAGTGGATTCGTCGGCATTGAAATGGACTTCGACTATAA
GTACAAAGGCAAGTTTTTAACTCTATTGGAAAAAGTCAATGGCTAA

Upstream 100 bases:

>100_bases
CTTATCTGTTATTATTGCTTATGGAGAAGACAAAATCGTTTAAAAATTCTATACTTAATAAAGCGGAATAGGTACATCAG
GTTGTGTTTTATTTCCGCAA

Downstream 100 bases:

>100_bases
AGAGTGGATCCTCAACAGCGCGATGAACCGTTTCCAGTTGAATTTCAAAAGGAATGTCGGCCCAACATCCGAATCCATCA
GGAAATGTGCACCCAAAACG

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.MjaI; N-4 cytosine-specific methyltransferase MjaI [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSITKCFKKSVLTTHHTIIQGDSRQMNLLSDRSIHLVITSPPYWQLKDYGTESQIGFHESYESYINNLNLVWSECERALY
PGCRLCVNIGDQFARSVYYGRYKVIPIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGSFSYPRNGILKLDYEF
ILIFKKLGDAPKPTKEQKERSAMTKEEWNTYFSGHWNFAGAKQDGHIAMFPEELPRRLIKMFAFEGDTVLDPFMGSGTTN
LAARNLGRNSVGYEINSEFVEISKQKLGVNQPDLVGTKYDFQYDTVSGDFEIAIQQLPYRFQDPHKLDKKVDPRKLSFGS
RIDKENGAQREELFTVKEALSPEKLLLSNGLTIRLLGIKSDPVLKDKAVGYLNEKIKGKRIFLKYDRKKYDEENNLLCYL
YLQNKTFVNAHLIRSGFVGIEMDFDYKYKGKFLTLLEKVNG

Sequences:

>Translated_441_residues
MSITKCFKKSVLTTHHTIIQGDSRQMNLLSDRSIHLVITSPPYWQLKDYGTESQIGFHESYESYINNLNLVWSECERALY
PGCRLCVNIGDQFARSVYYGRYKVIPIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGSFSYPRNGILKLDYEF
ILIFKKLGDAPKPTKEQKERSAMTKEEWNTYFSGHWNFAGAKQDGHIAMFPEELPRRLIKMFAFEGDTVLDPFMGSGTTN
LAARNLGRNSVGYEINSEFVEISKQKLGVNQPDLVGTKYDFQYDTVSGDFEIAIQQLPYRFQDPHKLDKKVDPRKLSFGS
RIDKENGAQREELFTVKEALSPEKLLLSNGLTIRLLGIKSDPVLKDKAVGYLNEKIKGKRIFLKYDRKKYDEENNLLCYL
YLQNKTFVNAHLIRSGFVGIEMDFDYKYKGKFLTLLEKVNG
>Mature_440_residues
SITKCFKKSVLTTHHTIIQGDSRQMNLLSDRSIHLVITSPPYWQLKDYGTESQIGFHESYESYINNLNLVWSECERALYP
GCRLCVNIGDQFARSVYYGRYKVIPIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGSFSYPRNGILKLDYEFI
LIFKKLGDAPKPTKEQKERSAMTKEEWNTYFSGHWNFAGAKQDGHIAMFPEELPRRLIKMFAFEGDTVLDPFMGSGTTNL
AARNLGRNSVGYEINSEFVEISKQKLGVNQPDLVGTKYDFQYDTVSGDFEIAIQQLPYRFQDPHKLDKKVDPRKLSFGSR
IDKENGAQREELFTVKEALSPEKLLLSNGLTIRLLGIKSDPVLKDKAVGYLNEKIKGKRIFLKYDRKKYDEENNLLCYLY
LQNKTFVNAHLIRSGFVGIEMDFDYKYKGKFLTLLEKVNG

Specific function: This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 50704; Mature: 50573

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITKCFKKSVLTTHHTIIQGDSRQMNLLSDRSIHLVITSPPYWQLKDYGTESQIGFHES
CCHHHHHHHHHHHHHHEEEECCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCCHHHH
YESYINNLNLVWSECERALYPGCRLCVNIGDQFARSVYYGRYKVIPIRTEIIRFCETIGF
HHHHHCCCHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCH
DYMGAVIWQKVTTTNTTGGASIMGSFSYPRNGILKLDYEFILIFKKLGDAPKPTKEQKER
HHHHHHHHHHEECCCCCCCCEEEECCCCCCCCEEEECHHEEEEEHHHCCCCCCCHHHHHH
SAMTKEEWNTYFSGHWNFAGAKQDGHIAMFPEELPRRLIKMFAFEGDTVLDPFMGSGTTN
HCCCHHHCCCEECCEEEECCCCCCCEEEECHHHHHHHHHHHHHCCCCCEECCCCCCCCCH
LAARNLGRNSVGYEINSEFVEISKQKLGVNQPDLVGTKYDFQYDTVSGDFEIAIQQLPYR
HHHHHCCCCCCCEEECHHHHHHHHHHCCCCCCCCCCCEECEEEECCCCCCEEHHHHCCCC
FQDPHKLDKKVDPRKLSFGSRIDKENGAQREELFTVKEALSPEKLLLSNGLTIRLLGIKS
CCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECC
DPVLKDKAVGYLNEKIKGKRIFLKYDRKKYDEENNLLCYLYLQNKTFVNAHLIRSGFVGI
CCCCCHHHHHHHHHCCCCCEEEEEECCHHCCCCCCEEEEEEECCCEEEEHEEHHCCCEEE
EMDFDYKYKGKFLTLLEKVNG
EEECCCEECCHHHHHHHHHCC
>Mature Secondary Structure 
SITKCFKKSVLTTHHTIIQGDSRQMNLLSDRSIHLVITSPPYWQLKDYGTESQIGFHES
CHHHHHHHHHHHHHHEEEECCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCCHHHH
YESYINNLNLVWSECERALYPGCRLCVNIGDQFARSVYYGRYKVIPIRTEIIRFCETIGF
HHHHHCCCHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCH
DYMGAVIWQKVTTTNTTGGASIMGSFSYPRNGILKLDYEFILIFKKLGDAPKPTKEQKER
HHHHHHHHHHEECCCCCCCCEEEECCCCCCCCEEEECHHEEEEEHHHCCCCCCCHHHHHH
SAMTKEEWNTYFSGHWNFAGAKQDGHIAMFPEELPRRLIKMFAFEGDTVLDPFMGSGTTN
HCCCHHHCCCEECCEEEECCCCCCCEEEECHHHHHHHHHHHHHCCCCCEECCCCCCCCCH
LAARNLGRNSVGYEINSEFVEISKQKLGVNQPDLVGTKYDFQYDTVSGDFEIAIQQLPYR
HHHHHCCCCCCCEEECHHHHHHHHHHCCCCCCCCCCCEECEEEECCCCCCEEHHHHCCCC
FQDPHKLDKKVDPRKLSFGSRIDKENGAQREELFTVKEALSPEKLLLSNGLTIRLLGIKS
CCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECC
DPVLKDKAVGYLNEKIKGKRIFLKYDRKKYDEENNLLCYLYLQNKTFVNAHLIRSGFVGI
CCCCCHHHHHHHHHCCCCCEEEEEECCHHCCCCCCEEEEEEECCCEEEEHEEHHCCCEEE
EMDFDYKYKGKFLTLLEKVNG
EEECCCEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]