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The map label for this gene is pckG [H]

Identifier: 189499033

GI number: 189499033

Start: 37806

End: 39665

Strand: Direct

Name: pckG [H]

Synonym: Cphamn1_0033

Alternate gene names: 189499033

Gene position: 37806-39665 (Clockwise)

Preceding gene: 189499032

Following gene: 189499034

Centisome position: 1.38

GC content: 51.88

Gene sequence:

>1860_bases
ATGCGCATCGATCCCCCCGATTATGTAAAAAACACAAAATTAATTGAGTGGGTACGTGAAACCGCGGAACTCTGTCAACC
GGATTCCATCATCTGGTGTGACGGCACACAGGAAGAATATGACACGTTGTGTCAGGAAATGGTTGATAGCGGTACCTTTA
CCAGACTATCGGATGAGAAGCGTCCGAACAGTTTCCTGTGCAGGTCTGATCCCAGTGACGTAGCCAGGGTTGAGGACAGG
ACCTATATTTGCAGCCTTCGCAGGCAGGATGCCGGTCCGACCAACAACTGGGTGCCGCCAAAAGAGATGAAAGCGACCCT
GAAAGAACTTTATTCTGGGTGTATGAAAGGGCGCACGATGTATGTCATTCCGTTCAGCATGGGTCCGCTTGGATCGCATA
TAGCGCACATTGGTGTAGAGATAACCGATTCACCATACGTCGTGGTGAATATGCGTATCATGACCAGAATGGGTAGAAAG
GTTATGGATATTCTCGCAGATGGCCGGGATTTCGTGCACTGTCTGCATTCGGTCGGCGCGCCGCTTGAAAAGGGTGCGCA
GGATGTCCCATGGCCTTGTAACGATCAGAAGTATATTGTCCATTTTCCTGAAGAGCGCTCAATAATGTCTTTCGGCAGCG
GATACGGAGGTAACGCGCTGCTTGGCAAAAAGTGTTTCGCTCTTCGAATCGCCTCTTCCATGGCTCGTGACGAGGGATGG
CTGGCAGAACATATGCTCATTCTTTGTGTTGAATCTCCCGATGGAGAAAAAACCTATGTGGCAGCCGCCTTCCCGAGTGC
ATGCGGTAAAACCAATTTTGCCATGCTTATTCCTCCGGACTCTTTTGAGGGATGGAAGGTTACTACGATTGGTGATGATA
TTGCCTGGATTAAGCCCGCTGAAGACGGCAGGCTCCACGCCATCAACCCTGAATACGGATTTTTCGGAGTGGCGCCCGGC
ACATCGGTAAAAACCAATCCGAACGCCATGGCGACCCTGAGTGCAAACTGTATTTATACCAATGTCGCATTGACGCCTGA
AGGCGATGTCTGGTGGGAAGGTATGACCGATACCCCTCCTGAGTCGCTCATTGACTGGCAGGGTAATGCATGGACTCCTG
ATGCAGGGAGGCCTGCAGCCCATCCGAACGCCCGTTTTACCTCGCCTGCTTCTCAGTGTCCGAGTATCGATCCCGAATGG
GAGAATCCGGCGGGTGTGCCGATCGACGCCTTTATTTTCGGTGGTAGGCGCAGTAATCTTGTGCCCCTTGTCTACCAGTC
GCCAAACTGGTATTTCGGAGTTTATCTTGCAGCTACCATGGGCTCTGAAAAGACGGCGGCGGCAGCTGGAAAAATCGGCG
AGGTGCGTCGGGATCCTTTTGCCATGCTCCCCTTCTGCGGCTACCATATGGGCGACTATTTCACCCATTGGCTTCATGTC
GGACGACTCCTTCAGGAGCCGCCAAGAATTTTCGGTGTTAACTGGTTCCGCAAAGATGAAAACGGCAAGTTTCTCTGGCC
CGGTTTTGGCGAGAACATGCGAGTGTTGAAGTGGATCGTCGATCGGGTGCATGGGCGTTCGAGCGCAGTGGAAAGTCCTC
TTGGCTGGATGCCTAAATATGAGTCCATGGAGTGGGATGGGCTCGACGAGTTCACTTTAGACGATTTTTCAAAGCTTATG
GCGGTTGATCGTGAAGACTGGAAGAAAGAGCTGTTGTCTCACGAGGCGCTTTTTGAGAAAATTTATGATAAACTGCCCAA
GGAGTTCTCGCATATACGGGAGCTGCTTCTCTCGACCTTCTGGCTCTCTCCTGAGCATTGGGGGCTTGCCCAGGAGCGCT
ATACGGGAGAGCATCACTGA

Upstream 100 bases:

>100_bases
GGGCAAGTAAGTTATGCTTGATGTTTCCGCATGATGGCTTGCACGTGTGATTTCGAGTATTCTTTTTTAACCTTCTGTAA
AACAGACGAACATGAGCTCA

Downstream 100 bases:

>100_bases
ACGCCCGATTTTGCGATCAATGATTTTTATCACAAGCCGCGACAGGATTTTCCCTGTCGTGGCTTTTTTTAACCTCTGCA
GGAAAGAAGGTCCATTTCTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MRIDPPDYVKNTKLIEWVRETAELCQPDSIIWCDGTQEEYDTLCQEMVDSGTFTRLSDEKRPNSFLCRSDPSDVARVEDR
TYICSLRRQDAGPTNNWVPPKEMKATLKELYSGCMKGRTMYVIPFSMGPLGSHIAHIGVEITDSPYVVVNMRIMTRMGRK
VMDILADGRDFVHCLHSVGAPLEKGAQDVPWPCNDQKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASSMARDEGW
LAEHMLILCVESPDGEKTYVAAAFPSACGKTNFAMLIPPDSFEGWKVTTIGDDIAWIKPAEDGRLHAINPEYGFFGVAPG
TSVKTNPNAMATLSANCIYTNVALTPEGDVWWEGMTDTPPESLIDWQGNAWTPDAGRPAAHPNARFTSPASQCPSIDPEW
ENPAGVPIDAFIFGGRRSNLVPLVYQSPNWYFGVYLAATMGSEKTAAAAGKIGEVRRDPFAMLPFCGYHMGDYFTHWLHV
GRLLQEPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIVDRVHGRSSAVESPLGWMPKYESMEWDGLDEFTLDDFSKLM
AVDREDWKKELLSHEALFEKIYDKLPKEFSHIRELLLSTFWLSPEHWGLAQERYTGEHH

Sequences:

>Translated_619_residues
MRIDPPDYVKNTKLIEWVRETAELCQPDSIIWCDGTQEEYDTLCQEMVDSGTFTRLSDEKRPNSFLCRSDPSDVARVEDR
TYICSLRRQDAGPTNNWVPPKEMKATLKELYSGCMKGRTMYVIPFSMGPLGSHIAHIGVEITDSPYVVVNMRIMTRMGRK
VMDILADGRDFVHCLHSVGAPLEKGAQDVPWPCNDQKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASSMARDEGW
LAEHMLILCVESPDGEKTYVAAAFPSACGKTNFAMLIPPDSFEGWKVTTIGDDIAWIKPAEDGRLHAINPEYGFFGVAPG
TSVKTNPNAMATLSANCIYTNVALTPEGDVWWEGMTDTPPESLIDWQGNAWTPDAGRPAAHPNARFTSPASQCPSIDPEW
ENPAGVPIDAFIFGGRRSNLVPLVYQSPNWYFGVYLAATMGSEKTAAAAGKIGEVRRDPFAMLPFCGYHMGDYFTHWLHV
GRLLQEPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIVDRVHGRSSAVESPLGWMPKYESMEWDGLDEFTLDDFSKLM
AVDREDWKKELLSHEALFEKIYDKLPKEFSHIRELLLSTFWLSPEHWGLAQERYTGEHH
>Mature_619_residues
MRIDPPDYVKNTKLIEWVRETAELCQPDSIIWCDGTQEEYDTLCQEMVDSGTFTRLSDEKRPNSFLCRSDPSDVARVEDR
TYICSLRRQDAGPTNNWVPPKEMKATLKELYSGCMKGRTMYVIPFSMGPLGSHIAHIGVEITDSPYVVVNMRIMTRMGRK
VMDILADGRDFVHCLHSVGAPLEKGAQDVPWPCNDQKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASSMARDEGW
LAEHMLILCVESPDGEKTYVAAAFPSACGKTNFAMLIPPDSFEGWKVTTIGDDIAWIKPAEDGRLHAINPEYGFFGVAPG
TSVKTNPNAMATLSANCIYTNVALTPEGDVWWEGMTDTPPESLIDWQGNAWTPDAGRPAAHPNARFTSPASQCPSIDPEW
ENPAGVPIDAFIFGGRRSNLVPLVYQSPNWYFGVYLAATMGSEKTAAAAGKIGEVRRDPFAMLPFCGYHMGDYFTHWLHV
GRLLQEPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIVDRVHGRSSAVESPLGWMPKYESMEWDGLDEFTLDDFSKLM
AVDREDWKKELLSHEALFEKIYDKLPKEFSHIRELLLSTFWLSPEHWGLAQERYTGEHH

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=592, Percent_Identity=52.7027027027027, Blast_Score=605, Evalue=1e-173,
Organism=Homo sapiens, GI187281517, Length=599, Percent_Identity=51.9198664440735, Blast_Score=597, Evalue=1e-171,
Organism=Homo sapiens, GI66346723, Length=386, Percent_Identity=53.3678756476684, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71989645, Length=592, Percent_Identity=48.8175675675676, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989658, Length=592, Percent_Identity=48.8175675675676, Blast_Score=562, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17555492, Length=601, Percent_Identity=48.2529118136439, Blast_Score=557, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989653, Length=578, Percent_Identity=49.1349480968858, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=536, Percent_Identity=51.1194029850746, Blast_Score=539, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI32564407, Length=509, Percent_Identity=50.4911591355599, Blast_Score=500, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71989649, Length=481, Percent_Identity=50.9355509355509, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI32564409, Length=338, Percent_Identity=48.8165680473373, Blast_Score=325, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17540956, Length=513, Percent_Identity=24.7563352826511, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24655085, Length=606, Percent_Identity=50.4950495049505, Blast_Score=606, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24655082, Length=596, Percent_Identity=51.510067114094, Blast_Score=588, Evalue=1e-168,
Organism=Drosophila melanogaster, GI161077226, Length=565, Percent_Identity=52.212389380531, Blast_Score=562, Evalue=1e-160,
Organism=Drosophila melanogaster, GI24655088, Length=505, Percent_Identity=53.0693069306931, Blast_Score=533, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 69855; Mature: 69855

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDPPDYVKNTKLIEWVRETAELCQPDSIIWCDGTQEEYDTLCQEMVDSGTFTRLSDEK
CCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEECCCCC
RPNSFLCRSDPSDVARVEDRTYICSLRRQDAGPTNNWVPPKEMKATLKELYSGCMKGRTM
CCCCEEECCCCHHHHHHCCCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
YVIPFSMGPLGSHIAHIGVEITDSPYVVVNMRIMTRMGRKVMDILADGRDFVHCLHSVGA
EEEEECCCCCHHHHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
PLEKGAQDVPWPCNDQKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASSMARDEGW
CHHHCCCCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
LAEHMLILCVESPDGEKTYVAAAFPSACGKTNFAMLIPPDSFEGWKVTTIGDDIAWIKPA
HEEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEECC
EDGRLHAINPEYGFFGVAPGTSVKTNPNAMATLSANCIYTNVALTPEGDVWWEGMTDTPP
CCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEECCCCCEEECCCCCCCH
ESLIDWQGNAWTPDAGRPAAHPNARFTSPASQCPSIDPEWENPAGVPIDAFIFGGRRSNL
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCE
VPLVYQSPNWYFGVYLAATMGSEKTAAAAGKIGEVRRDPFAMLPFCGYHMGDYFTHWLHV
EEEEEECCCEEEEEEEEEECCCCHHHHHHCCHHHHCCCCCEECCHHHHHHHHHHHHHHHH
GRLLQEPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIVDRVHGRSSAVESPLGWMPKY
HHHHHCCCCEECCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCC
ESMEWDGLDEFTLDDFSKLMAVDREDWKKELLSHEALFEKIYDKLPKEFSHIRELLLSTF
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSPEHWGLAQERYTGEHH
CCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MRIDPPDYVKNTKLIEWVRETAELCQPDSIIWCDGTQEEYDTLCQEMVDSGTFTRLSDEK
CCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEECCCCC
RPNSFLCRSDPSDVARVEDRTYICSLRRQDAGPTNNWVPPKEMKATLKELYSGCMKGRTM
CCCCEEECCCCHHHHHHCCCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
YVIPFSMGPLGSHIAHIGVEITDSPYVVVNMRIMTRMGRKVMDILADGRDFVHCLHSVGA
EEEEECCCCCHHHHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
PLEKGAQDVPWPCNDQKYIVHFPEERSIMSFGSGYGGNALLGKKCFALRIASSMARDEGW
CHHHCCCCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
LAEHMLILCVESPDGEKTYVAAAFPSACGKTNFAMLIPPDSFEGWKVTTIGDDIAWIKPA
HEEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEECC
EDGRLHAINPEYGFFGVAPGTSVKTNPNAMATLSANCIYTNVALTPEGDVWWEGMTDTPP
CCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEECCCCCEEECCCCCCCH
ESLIDWQGNAWTPDAGRPAAHPNARFTSPASQCPSIDPEWENPAGVPIDAFIFGGRRSNL
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCE
VPLVYQSPNWYFGVYLAATMGSEKTAAAAGKIGEVRRDPFAMLPFCGYHMGDYFTHWLHV
EEEEEECCCEEEEEEEEEECCCCHHHHHHCCHHHHCCCCCEECCHHHHHHHHHHHHHHHH
GRLLQEPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIVDRVHGRSSAVESPLGWMPKY
HHHHHCCCCEECCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCC
ESMEWDGLDEFTLDDFSKLMAVDREDWKKELLSHEALFEKIYDKLPKEFSHIRELLLSTF
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSPEHWGLAQERYTGEHH
CCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]