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The map label for this gene is mdh [H]

Identifier: 189352631

GI number: 189352631

Start: 719399

End: 720385

Strand: Reverse

Name: mdh [H]

Synonym: BMULJ_03862

Alternate gene names: 189352631

Gene position: 720385-719399 (Counterclockwise)

Preceding gene: 189352639

Following gene: 189352630

Centisome position: 29.13

GC content: 65.25

Gene sequence:

>987_bases
ATGGCTAAGCCCGCAAAGCGCGTTGCCGTCACCGGCGCCGCAGGTCAAATCGCTTACTCGCTGCTGTTCCGCATCGCGAA
CGGCGACCTGCTCGGCAAGGACCAGCCGGTCATTCTGCAACTGCTCGACCTCCCGCAAGCCCAAGCCGCCGTCAAAGGCG
TCGTGATGGAACTCGACGATTGCGCGTTCCCGCTGCTCGCGGGCGTCGTGATCACCGACGATCCGAAGGTTGCGTTCAAG
GACGCCGACGTCGCGCTGCTGGTCGGTGCACGTCCGCGCTCGAAGGGCATGGAGCGCAAGGATCTGCTGTCGGCGAACGC
GGAGATCTTCACGGTCCAGGGCGCGGCGCTGAACGAAGTCGCGAGCCGCGACGTGAAGGTGCTGGTCGTCGGCAACCCGG
CGAACACGAACGCCTACATCGCGATGAAGTCGGCGCCGGATCTGCCGAAGAAGAACTTCACCGCGATGCTGCGTCTGGAC
CACAACCGCGCGCTGTCGCAGCTCGCGGCGAAGTCGGGCAAGCCGGTCGCGTCGATCGAGAAGCTCGCCGTGTGGGGCAA
CCACTCGCCGACGATGTACCCCGACTTCCGCTTCGCGACGGCCGAAGGCGAATCGCTGCTGAAGCTGATCAACGACGACG
AATGGAACCGCAACACGTTCATCCCGACGGTCGGCAAGCGCGGCGCGGCGATCATCGAAGCGCGCGGCCTGTCGTCGGCG
GCGTCGGCAGCCAACGCGGCGATCGACCACGTGCGTGACTGGGTGCTCGGCACGAACGGCAAGTGGGTCACGATGGGCAT
TCCGTCGGACGGCTCGTACGGCATTCCCGAAGACATCATCTACGGCGTGCCGGTCACCTGCGAAAACGGCGAATACAAGC
GTGTCGAAGGCCTCGAGATCGACGCGTTCTCGCGCGAGAAGATGGACGCGACGCTCGCCGAGCTGCTCGAAGAGCGCGAC
GGCGTCGCTCACCTGCTGAAGAACTAA

Upstream 100 bases:

>100_bases
CGAGTCCTCCGCCCTGCTTTCAAAGCAACGCTTCGTGTAACGCGCATAGAATGGCGTTTTACCTAGCGTCCATCGCTTCA
TCCCGTCCTGGAGATTTTCA

Downstream 100 bases:

>100_bases
GCAGGCGGCATCGAGCGCCTGTCGGGCCGCGTGCGCGCGGCCCGGCACGGCCGGCCGGCACGCCGCCGCGCCGGCCATCC
GATCCGGACGCGTTTTGCCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQAAVKGVVMELDDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLD
HNRALSQLAAKSGKPVASIEKLAVWGNHSPTMYPDFRFATAEGESLLKLINDDEWNRNTFIPTVGKRGAAIIEARGLSSA
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDATLAELLEERD
GVAHLLKN

Sequences:

>Translated_328_residues
MAKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQAAVKGVVMELDDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLD
HNRALSQLAAKSGKPVASIEKLAVWGNHSPTMYPDFRFATAEGESLLKLINDDEWNRNTFIPTVGKRGAAIIEARGLSSA
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDATLAELLEERD
GVAHLLKN
>Mature_327_residues
AKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQAAVKGVVMELDDCAFPLLAGVVITDDPKVAFKD
ADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLDH
NRALSQLAAKSGKPVASIEKLAVWGNHSPTMYPDFRFATAEGESLLKLINDDEWNRNTFIPTVGKRGAAIIEARGLSSAA
SAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDATLAELLEERDG
VAHLLKN

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=334, Percent_Identity=53.8922155688623, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI89886456, Length=333, Percent_Identity=24.9249249249249, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17561064, Length=320, Percent_Identity=54.6875, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=257, Percent_Identity=54.863813229572, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=50, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=55.6923076923077, Blast_Score=340, Evalue=7e-94,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35161; Mature: 35029

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQAAVKGVVMELDD
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCHHHHHHHHHHEECCC
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCHHHHH
ASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLDHNRALSQLAAKSGKPVASIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHE
KLAVWGNHSPTMYPDFRFATAEGESLLKLINDDEWNRNTFIPTVGKRGAAIIEARGLSSA
EEEEECCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEI
HHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCHHHEECCEEEECCCCEEEECCEEE
DAFSREKMDATLAELLEERDGVAHLLKN
CCCCHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure 
AKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQAAVKGVVMELDD
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCHHHHHHHHHHEECCC
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCHHHHH
ASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLDHNRALSQLAAKSGKPVASIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHE
KLAVWGNHSPTMYPDFRFATAEGESLLKLINDDEWNRNTFIPTVGKRGAAIIEARGLSSA
EEEEECCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEI
HHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCHHHEECCEEEECCCCEEEECCEEE
DAFSREKMDATLAELLEERDGVAHLLKN
CCCCHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA