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The map label for this gene is prpD [H]

Identifier: 189352628

GI number: 189352628

Start: 716004

End: 717458

Strand: Reverse

Name: prpD [H]

Synonym: BMULJ_03859

Alternate gene names: 189352628

Gene position: 717458-716004 (Counterclockwise)

Preceding gene: 189352629

Following gene: 189352627

Centisome position: 29.01

GC content: 66.87

Gene sequence:

>1455_bases
ATGTCCGCTCCGGTCTCCAACGCCCGCCCCGCTCCGGATACGGTACTCGTCGACATCGTCGACTACGTGCTGAATGCCGG
TGCAATCGACAGCGCGCTCGCGCTGGAGACGGCGCGGCATTGCCTGATCGACACGCTGGGCTGCGGACTCGAGGCGCTGT
CCTACCCTGCCTGCACCAAGCTGCTCGGGCCCGTCGTGCCCGGCACGATCGTGCCGAACGGCGCGAAGGTGCCCGGCACC
TCGTTCCAGCTGGACCCGGTCAAGGCCGCGTTCGACATCGGCGCGACGATCCGCTGGCTCGACTTCAACGACACCTGGCT
CGCCGCCGAATGGGGTCATCCGTCCGACAACCTGGGCGGCATCCTGGCGACGGCCGACTGGCTGTCCCGCACCGCCGTCG
CGGCCGGCAAGGCGCCGCTCGCGATGCGCGACGTGCTGATCGCGATGATTCAGGCCCACGAGATCCAGGGCTGTCTCGCA
CTCGAAAATTCGTTCAACGCGGTCGGGCTCGACCACGTGCTGCTCGTGAAGGTCGCCTCGACGGCCGTCGTCGGGCGGCT
GCTCGGGCTCACGCGCGACGAGCTGATCAATGCGGTATCGAACGCGTTCGTCGACGGACATCCGCTGCGCACCTACCGCC
ACGCGCCGAACACCGGTTCGCGCAAGTCGTGGGCGGCCGGCGACGCGACCTCCCGCGCGGTGCGCCTCGCGCTGATCGCG
AAGACGGGTGAAATGGGCTATCCGTCCGCGCTGAGCGCGCGCACGTGGGGCTTCTACGACGTGCTGTTCGGCGGCAAGCC
GTTCCGCTTCCAGCGTCCCTACGGCACCTACGTGATGGAAAACGTGCTGTTCAAGATCGCGTTTCCCGCCGAATTCCATG
CGCAGACGGCCGTCGAGGCCGCGCTGCAGCTGCACGATGCGCTGGCCGCGGCCGGCCGCTCGACCGACGAGATCGCGCGG
ATCACGATCCGCACGCACGCCGCCGCGATCCGCATCATCGACAAGCAGGGGCCGCTCGCGAATCCCGCCGATCGCGACCA
CTGCATTCAATACATGGTTGCCGTGCCGCTGCTGTTCGGCCGGCTGACCGCTGCCGACTACGAAGACGCGGTCGCCGCCG
ACCCGCGCATCGACGCGCTGCGCGCGAAGACGATCTGCGTCGAAGATCCGCAGTTCACGAAGGACTATCACGATCCGAAC
AAGCGGTCGATCGCGAATGCGCTGACGATCGAGTTCGCGAACGGATCGACGCTTGCCGAAGTCGCGGTCGAATATCCGAT
CGGCCACCGGCGGCGCCGCGCCGACGGCATCCCGCTGCTGGTCGACAAGTTCCGCACGAACCTTGCGCGCCGCTTTCCGG
CAAAGCAGCAACAAGCGATTCTCGACGTGTCGCTGGACCAGGCAAAGCTCGAAGCGATGCCGGTCAATGAATACGTCGAT
CTGTATATGATCTAG

Upstream 100 bases:

>100_bases
AATGCGTGACGCCGGAAATGGCGCAAGGCGGCGCCGCACCGACGCAGGCGAACGAGCTGAAGCCGGCCGTCAACTAAGGA
CGCTCCGCGCAGGCGCCTCG

Downstream 100 bases:

>100_bases
CCGTCAGGTACAGAACAAACAGGAGCTCTTCTTTCCTCGCCTTAACCCAGGAAAATCACCATGGCCCACAATCTGCACAA
GACCCTCAAGGAATTCGACA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSAPVSNARPAPDTVLVDIVDYVLNAGAIDSALALETARHCLIDTLGCGLEALSYPACTKLLGPVVPGTIVPNGAKVPGT
SFQLDPVKAAFDIGATIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVAAGKAPLAMRDVLIAMIQAHEIQGCLA
LENSFNAVGLDHVLLVKVASTAVVGRLLGLTRDELINAVSNAFVDGHPLRTYRHAPNTGSRKSWAAGDATSRAVRLALIA
KTGEMGYPSALSARTWGFYDVLFGGKPFRFQRPYGTYVMENVLFKIAFPAEFHAQTAVEAALQLHDALAAAGRSTDEIAR
ITIRTHAAAIRIIDKQGPLANPADRDHCIQYMVAVPLLFGRLTAADYEDAVAADPRIDALRAKTICVEDPQFTKDYHDPN
KRSIANALTIEFANGSTLAEVAVEYPIGHRRRRADGIPLLVDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVNEYVD
LYMI

Sequences:

>Translated_484_residues
MSAPVSNARPAPDTVLVDIVDYVLNAGAIDSALALETARHCLIDTLGCGLEALSYPACTKLLGPVVPGTIVPNGAKVPGT
SFQLDPVKAAFDIGATIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVAAGKAPLAMRDVLIAMIQAHEIQGCLA
LENSFNAVGLDHVLLVKVASTAVVGRLLGLTRDELINAVSNAFVDGHPLRTYRHAPNTGSRKSWAAGDATSRAVRLALIA
KTGEMGYPSALSARTWGFYDVLFGGKPFRFQRPYGTYVMENVLFKIAFPAEFHAQTAVEAALQLHDALAAAGRSTDEIAR
ITIRTHAAAIRIIDKQGPLANPADRDHCIQYMVAVPLLFGRLTAADYEDAVAADPRIDALRAKTICVEDPQFTKDYHDPN
KRSIANALTIEFANGSTLAEVAVEYPIGHRRRRADGIPLLVDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVNEYVD
LYMI
>Mature_483_residues
SAPVSNARPAPDTVLVDIVDYVLNAGAIDSALALETARHCLIDTLGCGLEALSYPACTKLLGPVVPGTIVPNGAKVPGTS
FQLDPVKAAFDIGATIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVAAGKAPLAMRDVLIAMIQAHEIQGCLAL
ENSFNAVGLDHVLLVKVASTAVVGRLLGLTRDELINAVSNAFVDGHPLRTYRHAPNTGSRKSWAAGDATSRAVRLALIAK
TGEMGYPSALSARTWGFYDVLFGGKPFRFQRPYGTYVMENVLFKIAFPAEFHAQTAVEAALQLHDALAAAGRSTDEIARI
TIRTHAAAIRIIDKQGPLANPADRDHCIQYMVAVPLLFGRLTAADYEDAVAADPRIDALRAKTICVEDPQFTKDYHDPNK
RSIANALTIEFANGSTLAEVAVEYPIGHRRRRADGIPLLVDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVNEYVDL
YMI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=484, Percent_Identity=75.8264462809917, Blast_Score=783, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=60.082304526749, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52311; Mature: 52180

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVSNARPAPDTVLVDIVDYVLNAGAIDSALALETARHCLIDTLGCGLEALSYPACTK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHH
LLGPVVPGTIVPNGAKVPGTSFQLDPVKAAFDIGATIRWLDFNDTWLAAEWGHPSDNLGG
HHHCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCC
ILATADWLSRTAVAAGKAPLAMRDVLIAMIQAHEIQGCLALENSFNAVGLDHVLLVKVAS
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHH
TAVVGRLLGLTRDELINAVSNAFVDGHPLRTYRHAPNTGSRKSWAAGDATSRAVRLALIA
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCHHHEEEEEEEE
KTGEMGYPSALSARTWGFYDVLFGGKPFRFQRPYGTYVMENVLFKIAFPAEFHAQTAVEA
ECCCCCCCCCCCCCCCCCEEHHCCCCCEEEECCCHHHHHHHHHEEEECCHHHHHHHHHHH
ALQLHDALAAAGRSTDEIARITIRTHAAAIRIIDKQGPLANPADRDHCIQYMVAVPLLFG
HHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RLTAADYEDAVAADPRIDALRAKTICVEDPQFTKDYHDPNKRSIANALTIEFANGSTLAE
HHHCCCCCHHHCCCCCHHHHHCCEEEECCCCCCCCCCCCCHHHHCCEEEEEECCCCCHHH
VAVEYPIGHRRRRADGIPLLVDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVNEYVD
HHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHEEECCCCCEE
LYMI
EEEC
>Mature Secondary Structure 
SAPVSNARPAPDTVLVDIVDYVLNAGAIDSALALETARHCLIDTLGCGLEALSYPACTK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHH
LLGPVVPGTIVPNGAKVPGTSFQLDPVKAAFDIGATIRWLDFNDTWLAAEWGHPSDNLGG
HHHCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCC
ILATADWLSRTAVAAGKAPLAMRDVLIAMIQAHEIQGCLALENSFNAVGLDHVLLVKVAS
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHH
TAVVGRLLGLTRDELINAVSNAFVDGHPLRTYRHAPNTGSRKSWAAGDATSRAVRLALIA
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCHHHEEEEEEEE
KTGEMGYPSALSARTWGFYDVLFGGKPFRFQRPYGTYVMENVLFKIAFPAEFHAQTAVEA
ECCCCCCCCCCCCCCCCCEEHHCCCCCEEEECCCHHHHHHHHHEEEECCHHHHHHHHHHH
ALQLHDALAAAGRSTDEIARITIRTHAAAIRIIDKQGPLANPADRDHCIQYMVAVPLLFG
HHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RLTAADYEDAVAADPRIDALRAKTICVEDPQFTKDYHDPNKRSIANALTIEFANGSTLAE
HHHCCCCCHHHCCCCCHHHHHCCEEEECCCCCCCCCCCCCHHHHCCEEEEEECCCCCHHH
VAVEYPIGHRRRRADGIPLLVDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVNEYVD
HHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHEEECCCCCEE
LYMI
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]