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The map label for this gene is epsB [H]

Identifier: 189352380

GI number: 189352380

Start: 435125

End: 437350

Strand: Direct

Name: epsB [H]

Synonym: BMULJ_03599

Alternate gene names: 189352380

Gene position: 435125-437350 (Clockwise)

Preceding gene: 189352379

Following gene: 189352381

Centisome position: 17.59

GC content: 68.06

Gene sequence:

>2226_bases
ATGGTGAACACGCAAGCGAAACACTCCTACGCGGATCCGACCGCGAAGACCGACGAAGAGGACGTCGTCCTCGGCCAGCT
GCTGCAGGTGATCCTCGACGACATCTGGCTGCTGCTCGGCATCGCGGTGGCGGTCGTCGCGCTCGCCGGCCTCTACTGCT
ACGTCGCCAAGCCGGTGTATCAGGCCGACGTGCACGTGCGCGTCGAAGGCAACGACAACACGACGCAGGCGCTCACGCAG
ACGCAGACCGGCGCGATGATCAACAGCGGCCCGCAGCAGGCGCCGACCGACGCGGAGATCGAGATCATCAAGAGCCGCGG
CGTCGTCGCGCCGGTCGTCGAGCAGTTCAAGCTGAACTTCTCGGTCGTGCCGAAGACGATCCCGCTGCTCGGCAGCCTCA
CGGCGCGGCTCGCGACGCCCGGCGAACCGGCGCGTCCGTGGCTCGGCCTGAAATCGTACGCGTGGGGCGGCGAGGTCGCC
GACATCGACTCGATCAACGTCGTGCCCGCGCTCGAAGGCAAGAAGCTGACGCTGACGGCCGGGCCGAACGGCAACTACGT
GCTGACCGACGAGAACGGCATGCGGCTGCTGACCGGCCGCGTCGGCGAGTCGGCGCAGGGCGGCGGCGTCACGCTGCTCG
TGCAGAAGCTCGTCGCGCGCCCCGGCACGCAGTTTACGGTCACGCGCTACAACGATCTCGACGCGATCACCGGCTTCCAG
TCGGGCATCCAGGTGTCCGAGCAGGGCAAGCAGACGGGCGTCGTGCAGATCTCGCTCGAAGGCAAGGATCCGGAGCAGAC
TGCCGCGATCGCCAACGCGCTCGCGCAGTCGTATCTGCACCAGCACGTGATCGCGAAGCAGGCCGAGGCGACCAAGATGC
TCGACTTCCTGAAGGGCGAGGAACCGCGCCTGAAGGCCGATCTCGAACGCGCGGAAGCGGCGCTGACGCAGTATCAGCGC
ACGTCCGGCTCGATCAACGCGAGCGACGAGGCGAAGGTCTATCTCGAAGGCAGCGTGCAGTACGAACAGCAGATCGCCGC
GCAGCGGCTGCAGCTCGCGTCGCTCGCGCAGCGCTTCACCGATTCGCATCCGCTCGTGATCGCCGCGAAGCAGCAGCTCG
CGGAGCTGCAGGGCGAGAAGGAGAAATTCAGCAACCGCTTCCGCAGCCTGCCGGCCACCGAAGTGAAGGCCGTGCAGCTG
CAGCGCGACGCGAAGGTCGCCGAAGACATCTACGTGCTGCTGCTGAACCGCGTGCAGGAGCTGTCGGTGCAGAAGGCCGG
CACGGGCGGCAACATCCATCTGATCGACTCGGCGCTGCGTCCGGGCGCGCCGGTCAAGCCGAAGAAGGTGCTGATCCTGT
CGGCCGCGGTGTTCCTCGGGCTGATCCTCGGCACGGGCGTCGTGTTCCTGCGCCGCAACCTGTTCCAGGGGATCGAGGAT
CCGGACCGCATCGAACGCGCGTTCAACCTGCCGCTCTACGGGCTCGTGCCGCAAAGCGCCGAACAGGCGAAGCTCGACGC
GGCGGCCGAGAAGAGCGGCGGCCGTACGCGGCCGATCCTGGCGAGCCTGCGGCCGAAGGATCTCAGCGTCGAAAGCCTGC
GCAGCCTGCGCACCGCGATGCAGTTCGCGATGATGGACGCGAAGAACCGCGTGATCGTGCTGACGGGGCCGACGCCCGGC
ATCGGCAAGAGCTTCCTGACCGTGAACCTCGCGGTGCTGCTCGCGCACTCGGGCAAGCGCGTGCTGCTGATCGACGCCGA
CATGCGCCGCGGCGTGCTCGACCGCTACTTCGGGCTGACCGCGCAGCCGGGCATGTCGGAACTGCTGAGCGGCCAGTCGG
CGCTCGAGGAAGCGGTGCGCGAAACGCCGGTGCAGGGGCTCTCGTTCATCGCGGCCGGCACGCGTCCGCCGAATCCGTCG
GAGTTGCTGATGTCGGCGCGTCTGCCGCAATACCTCGAAGGGCTCGGCAAGCGCTACGACGTCGTGCTGATCGATTCGCC
GCCGGTGCTCGCGGTGACCGACGCGACGATCATCGGCCGCATGGCGGGCTCGACGTTCCTCGTGCTGCGCTCGGGCATGC
ATACGGCGGGCGAGATCGCGGACGCGATCAAGCGGCTGCGCACCGCGGGCGTCGATCTCGAAGGCGGGATCTTCAACGGC
GTGGCGCCGAAGACGCGCGGCTACGGCCGCGGCTATGCGGCCGTCCACGAATACCTGAGCGCATGA

Upstream 100 bases:

>100_bases
AGACGATCTTCTACATGCGGCAGATCACGCGCTGACGCCGCACAGGGCGGCCATCCGCCGGGGCGGCGTCTGCGCCCCGG
CACCACTCAAGCGGGAACGA

Downstream 100 bases:

>100_bases
ACGCGCTGCACGACACCGGGCCGTCGCGCGATGCGGTCGCCCGGCCGACGGGAGAACCGACCATGAAAATTTCCGTGCTC
GTTCCGACCTACCGGCGCCC

Product: protein-tyrosine kinase

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: EPS I polysaccharide export protein epsB [H]

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MVNTQAKHSYADPTAKTDEEDVVLGQLLQVILDDIWLLLGIAVAVVALAGLYCYVAKPVYQADVHVRVEGNDNTTQALTQ
TQTGAMINSGPQQAPTDAEIEIIKSRGVVAPVVEQFKLNFSVVPKTIPLLGSLTARLATPGEPARPWLGLKSYAWGGEVA
DIDSINVVPALEGKKLTLTAGPNGNYVLTDENGMRLLTGRVGESAQGGGVTLLVQKLVARPGTQFTVTRYNDLDAITGFQ
SGIQVSEQGKQTGVVQISLEGKDPEQTAAIANALAQSYLHQHVIAKQAEATKMLDFLKGEEPRLKADLERAEAALTQYQR
TSGSINASDEAKVYLEGSVQYEQQIAAQRLQLASLAQRFTDSHPLVIAAKQQLAELQGEKEKFSNRFRSLPATEVKAVQL
QRDAKVAEDIYVLLLNRVQELSVQKAGTGGNIHLIDSALRPGAPVKPKKVLILSAAVFLGLILGTGVVFLRRNLFQGIED
PDRIERAFNLPLYGLVPQSAEQAKLDAAAEKSGGRTRPILASLRPKDLSVESLRSLRTAMQFAMMDAKNRVIVLTGPTPG
IGKSFLTVNLAVLLAHSGKRVLLIDADMRRGVLDRYFGLTAQPGMSELLSGQSALEEAVRETPVQGLSFIAAGTRPPNPS
ELLMSARLPQYLEGLGKRYDVVLIDSPPVLAVTDATIIGRMAGSTFLVLRSGMHTAGEIADAIKRLRTAGVDLEGGIFNG
VAPKTRGYGRGYAAVHEYLSA

Sequences:

>Translated_741_residues
MVNTQAKHSYADPTAKTDEEDVVLGQLLQVILDDIWLLLGIAVAVVALAGLYCYVAKPVYQADVHVRVEGNDNTTQALTQ
TQTGAMINSGPQQAPTDAEIEIIKSRGVVAPVVEQFKLNFSVVPKTIPLLGSLTARLATPGEPARPWLGLKSYAWGGEVA
DIDSINVVPALEGKKLTLTAGPNGNYVLTDENGMRLLTGRVGESAQGGGVTLLVQKLVARPGTQFTVTRYNDLDAITGFQ
SGIQVSEQGKQTGVVQISLEGKDPEQTAAIANALAQSYLHQHVIAKQAEATKMLDFLKGEEPRLKADLERAEAALTQYQR
TSGSINASDEAKVYLEGSVQYEQQIAAQRLQLASLAQRFTDSHPLVIAAKQQLAELQGEKEKFSNRFRSLPATEVKAVQL
QRDAKVAEDIYVLLLNRVQELSVQKAGTGGNIHLIDSALRPGAPVKPKKVLILSAAVFLGLILGTGVVFLRRNLFQGIED
PDRIERAFNLPLYGLVPQSAEQAKLDAAAEKSGGRTRPILASLRPKDLSVESLRSLRTAMQFAMMDAKNRVIVLTGPTPG
IGKSFLTVNLAVLLAHSGKRVLLIDADMRRGVLDRYFGLTAQPGMSELLSGQSALEEAVRETPVQGLSFIAAGTRPPNPS
ELLMSARLPQYLEGLGKRYDVVLIDSPPVLAVTDATIIGRMAGSTFLVLRSGMHTAGEIADAIKRLRTAGVDLEGGIFNG
VAPKTRGYGRGYAAVHEYLSA
>Mature_741_residues
MVNTQAKHSYADPTAKTDEEDVVLGQLLQVILDDIWLLLGIAVAVVALAGLYCYVAKPVYQADVHVRVEGNDNTTQALTQ
TQTGAMINSGPQQAPTDAEIEIIKSRGVVAPVVEQFKLNFSVVPKTIPLLGSLTARLATPGEPARPWLGLKSYAWGGEVA
DIDSINVVPALEGKKLTLTAGPNGNYVLTDENGMRLLTGRVGESAQGGGVTLLVQKLVARPGTQFTVTRYNDLDAITGFQ
SGIQVSEQGKQTGVVQISLEGKDPEQTAAIANALAQSYLHQHVIAKQAEATKMLDFLKGEEPRLKADLERAEAALTQYQR
TSGSINASDEAKVYLEGSVQYEQQIAAQRLQLASLAQRFTDSHPLVIAAKQQLAELQGEKEKFSNRFRSLPATEVKAVQL
QRDAKVAEDIYVLLLNRVQELSVQKAGTGGNIHLIDSALRPGAPVKPKKVLILSAAVFLGLILGTGVVFLRRNLFQGIED
PDRIERAFNLPLYGLVPQSAEQAKLDAAAEKSGGRTRPILASLRPKDLSVESLRSLRTAMQFAMMDAKNRVIVLTGPTPG
IGKSFLTVNLAVLLAHSGKRVLLIDADMRRGVLDRYFGLTAQPGMSELLSGQSALEEAVRETPVQGLSFIAAGTRPPNPS
ELLMSARLPQYLEGLGKRYDVVLIDSPPVLAVTDATIIGRMAGSTFLVLRSGMHTAGEIADAIKRLRTAGVDLEGGIFNG
VAPKTRGYGRGYAAVHEYLSA

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family [H]

Homologues:

Organism=Escherichia coli, GI87082032, Length=741, Percent_Identity=34.1430499325236, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1787216, Length=724, Percent_Identity=33.1491712707182, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR005700
- InterPro:   IPR003856 [H]

Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 79593; Mature: 79593

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTQAKHSYADPTAKTDEEDVVLGQLLQVILDDIWLLLGIAVAVVALAGLYCYVAKPVY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
QADVHVRVEGNDNTTQALTQTQTGAMINSGPQQAPTDAEIEIIKSRGVVAPVVEQFKLNF
ECEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCE
SVVPKTIPLLGSLTARLATPGEPARPWLGLKSYAWGGEVADIDSINVVPALEGKKLTLTA
EECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEE
GPNGNYVLTDENGMRLLTGRVGESAQGGGVTLLVQKLVARPGTQFTVTRYNDLDAITGFQ
CCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
SGIQVSEQGKQTGVVQISLEGKDPEQTAAIANALAQSYLHQHVIAKQAEATKMLDFLKGE
HCCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPRLKADLERAEAALTQYQRTSGSINASDEAKVYLEGSVQYEQQIAAQRLQLASLAQRFT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
DSHPLVIAAKQQLAELQGEKEKFSNRFRSLPATEVKAVQLQRDAKVAEDIYVLLLNRVQE
CCCCEEEEEHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVQKAGTGGNIHLIDSALRPGAPVKPKKVLILSAAVFLGLILGTGVVFLRRNLFQGIED
HHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDRIERAFNLPLYGLVPQSAEQAKLDAAAEKSGGRTRPILASLRPKDLSVESLRSLRTAM
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHH
QFAMMDAKNRVIVLTGPTPGIGKSFLTVNLAVLLAHSGKRVLLIDADMRRGVLDRYFGLT
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHCCCC
AQPGMSELLSGQSALEEAVRETPVQGLSFIAAGTRPPNPSELLMSARLPQYLEGLGKRYD
CCCCHHHHHCCHHHHHHHHHHCCCHHHHEEECCCCCCCHHHHHHHHCCHHHHHHCCCCEE
VVLIDSPPVLAVTDATIIGRMAGSTFLVLRSGMHTAGEIADAIKRLRTAGVDLEGGIFNG
EEEECCCCEEEEECHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
VAPKTRGYGRGYAAVHEYLSA
CCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MVNTQAKHSYADPTAKTDEEDVVLGQLLQVILDDIWLLLGIAVAVVALAGLYCYVAKPVY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
QADVHVRVEGNDNTTQALTQTQTGAMINSGPQQAPTDAEIEIIKSRGVVAPVVEQFKLNF
ECEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCE
SVVPKTIPLLGSLTARLATPGEPARPWLGLKSYAWGGEVADIDSINVVPALEGKKLTLTA
EECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEE
GPNGNYVLTDENGMRLLTGRVGESAQGGGVTLLVQKLVARPGTQFTVTRYNDLDAITGFQ
CCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
SGIQVSEQGKQTGVVQISLEGKDPEQTAAIANALAQSYLHQHVIAKQAEATKMLDFLKGE
HCCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPRLKADLERAEAALTQYQRTSGSINASDEAKVYLEGSVQYEQQIAAQRLQLASLAQRFT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
DSHPLVIAAKQQLAELQGEKEKFSNRFRSLPATEVKAVQLQRDAKVAEDIYVLLLNRVQE
CCCCEEEEEHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVQKAGTGGNIHLIDSALRPGAPVKPKKVLILSAAVFLGLILGTGVVFLRRNLFQGIED
HHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDRIERAFNLPLYGLVPQSAEQAKLDAAAEKSGGRTRPILASLRPKDLSVESLRSLRTAM
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHH
QFAMMDAKNRVIVLTGPTPGIGKSFLTVNLAVLLAHSGKRVLLIDADMRRGVLDRYFGLT
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHCCCC
AQPGMSELLSGQSALEEAVRETPVQGLSFIAAGTRPPNPSELLMSARLPQYLEGLGKRYD
CCCCHHHHHCCHHHHHHHHHHCCCHHHHEEECCCCCCCHHHHHHHHCCHHHHHHCCCCEE
VVLIDSPPVLAVTDATIIGRMAGSTFLVLRSGMHTAGEIADAIKRLRTAGVDLEGGIFNG
EEEECCCCEEEEECHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
VAPKTRGYGRGYAAVHEYLSA
CCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]