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The map label for this gene is wcaJ [H]

Identifier: 189352376

GI number: 189352376

Start: 430555

End: 431928

Strand: Direct

Name: wcaJ [H]

Synonym: BMULJ_03595

Alternate gene names: 189352376

Gene position: 430555-431928 (Clockwise)

Preceding gene: 189352374

Following gene: 189352377

Centisome position: 17.41

GC content: 64.77

Gene sequence:

>1374_bases
ATGTTGAGCGTGCTGGCGAGAGTCATCGATATCGCGATGGTCGTGGCGGGAGCGCTGCTCGCCGCCGCGCTGCATCGCGG
CAACGTGTGGTTCAGCGACCTGCAGCGCACGATGGTGCTGTTCGACTGCCTGCTCGTCGTCGTGTGCTTTCCCGCATTCG
GCATCTATCAGTCCTGGCGCGGCAAGCGGCTCGTCGGGCTGATGGGCCGCGTCGCGTTCGCATGGCTCGTCGTCGAGCTC
GCGGGCATCCTGATGAGTTTCAGCTTTCATCAGTCGGGCGAACTGTCGCGGCTCTGGCTCGGCTACTGGGCGATCGCGAC
GGTCACGCTGCTCGCCGGCTCGAAGGCGTGCGTGCATGCGGTGCTGCGGCGGCTGCGGCGCGGCGGCTTCAACCTGAAGG
CGGTCGCGATCGTCGGCGGCACGCCGGCCGCGCGGCGGCTGATTGCGCAGATGCGCGCGCGTCCGGAAGCGGGCTTCAAC
CCGGTGTGCGTGTACGACGAGGACGCGGCGCCCGGCGACGTCGCGCTCGACGACGTGCGTATCGAGCGGCAGTTCGAATC
GCTGGTCTGGCTCGTGCGTAGCCGCGCGATTCGCGAGCTGTGGCTGACCTTGCCGATCTCGGAAGAGCGGCGCATTCATC
AGATCGTCACGGTGTTTCGTCATGACTTCGTGAACATCCGTTTCATTCCGGACGTGCGCAGCCTGTCGTTCTTCAACCAG
GAAGTGGTCGAGGTGCTCGGCGTGCCGGCGATCAACCTCGCGGCGTCGCCGATCACCGACGTGCGGATCCTGCCGAAGTT
CGTGTTCGACCGGATGTTCGCGCTTGCCGCGCTCGTGATGCTCGCACCGCTGATGCTCGTGATCGCGTGCCTCATCAAGG
CGACCTCGCCGGGGCCCGTGTTCTTCCGGCAGAAGCGCAAGGGCATCGACGGGCACGAGTTCGAGATCTACAAGTTCCGC
TCGATGAAGGTGCATCAGGAAGCGGCGGGCACGGTGACGCAGGCAACGAAGAACGACTCGCGCGTGACGCCGGTCGGCCG
GTTCCTGCGCCGCACGAGCCTCGACGAACTGCCGCAGTTCATCAACGTGCTGAAGGGCGAGATGTCGGTCGTCGGTCCGC
GTCCGCATGCGCTCGCGCACGACGACATCTACAAGGATTTGGTCAAGGGCTACATGTTCCGCTATCGGATCAAGCCCGGC
ATCACCGGCTGGGCGCAGATCAACGGCTATCGCGGCGAGACCGACCAGATCGAGAAGATGATGGGGCGCGTGAAGCTCGA
TCTGTACTACATGCAGAACTGGTCGTTCTGGCTCGACATCAAGATCGTCGCGCTGACGCTGTGGAAAGGCTTCACGGGCA
GCAACGCGTACTGA

Upstream 100 bases:

>100_bases
CTTGTCGGTTCAATAGCGACATGCAACGTTTGAATCGAAGGTGCGGCACGGGCTGCACGAGGAGCTGTTCGGCAAACGAC
TGTTCAAAGAGGAAGCGGAC

Downstream 100 bases:

>100_bases
CGCGGCGCCGCATGCGCGGCTTCACGAATTTCGAATCATTGGTCAAGAGGTCCGACACATCATGAATCTGACTATCATCG
GCAGCGGTTACGTAGGTCTG

Product: putative colanic acid biosynthesis UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLSVLARVIDIAMVVAGALLAAALHRGNVWFSDLQRTMVLFDCLLVVVCFPAFGIYQSWRGKRLVGLMGRVAFAWLVVEL
AGILMSFSFHQSGELSRLWLGYWAIATVTLLAGSKACVHAVLRRLRRGGFNLKAVAIVGGTPAARRLIAQMRARPEAGFN
PVCVYDEDAAPGDVALDDVRIERQFESLVWLVRSRAIRELWLTLPISEERRIHQIVTVFRHDFVNIRFIPDVRSLSFFNQ
EVVEVLGVPAINLAASPITDVRILPKFVFDRMFALAALVMLAPLMLVIACLIKATSPGPVFFRQKRKGIDGHEFEIYKFR
SMKVHQEAAGTVTQATKNDSRVTPVGRFLRRTSLDELPQFINVLKGEMSVVGPRPHALAHDDIYKDLVKGYMFRYRIKPG
ITGWAQINGYRGETDQIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY

Sequences:

>Translated_457_residues
MLSVLARVIDIAMVVAGALLAAALHRGNVWFSDLQRTMVLFDCLLVVVCFPAFGIYQSWRGKRLVGLMGRVAFAWLVVEL
AGILMSFSFHQSGELSRLWLGYWAIATVTLLAGSKACVHAVLRRLRRGGFNLKAVAIVGGTPAARRLIAQMRARPEAGFN
PVCVYDEDAAPGDVALDDVRIERQFESLVWLVRSRAIRELWLTLPISEERRIHQIVTVFRHDFVNIRFIPDVRSLSFFNQ
EVVEVLGVPAINLAASPITDVRILPKFVFDRMFALAALVMLAPLMLVIACLIKATSPGPVFFRQKRKGIDGHEFEIYKFR
SMKVHQEAAGTVTQATKNDSRVTPVGRFLRRTSLDELPQFINVLKGEMSVVGPRPHALAHDDIYKDLVKGYMFRYRIKPG
ITGWAQINGYRGETDQIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY
>Mature_457_residues
MLSVLARVIDIAMVVAGALLAAALHRGNVWFSDLQRTMVLFDCLLVVVCFPAFGIYQSWRGKRLVGLMGRVAFAWLVVEL
AGILMSFSFHQSGELSRLWLGYWAIATVTLLAGSKACVHAVLRRLRRGGFNLKAVAIVGGTPAARRLIAQMRARPEAGFN
PVCVYDEDAAPGDVALDDVRIERQFESLVWLVRSRAIRELWLTLPISEERRIHQIVTVFRHDFVNIRFIPDVRSLSFFNQ
EVVEVLGVPAINLAASPITDVRILPKFVFDRMFALAALVMLAPLMLVIACLIKATSPGPVFFRQKRKGIDGHEFEIYKFR
SMKVHQEAAGTVTQATKNDSRVTPVGRFLRRTSLDELPQFINVLKGEMSVVGPRPHALAHDDIYKDLVKGYMFRYRIKPG
ITGWAQINGYRGETDQIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=462, Percent_Identity=39.6103896103896, Blast_Score=321, Evalue=5e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51590; Mature: 51590

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVLARVIDIAMVVAGALLAAALHRGNVWFSDLQRTMVLFDCLLVVVCFPAFGIYQSWR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKRLVGLMGRVAFAWLVVELAGILMSFSFHQSGELSRLWLGYWAIATVTLLAGSKACVHA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
VLRRLRRGGFNLKAVAIVGGTPAARRLIAQMRARPEAGFNPVCVYDEDAAPGDVALDDVR
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHH
IERQFESLVWLVRSRAIRELWLTLPISEERRIHQIVTVFRHDFVNIRFIPDVRSLSFFNQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHH
EVVEVLGVPAINLAASPITDVRILPKFVFDRMFALAALVMLAPLMLVIACLIKATSPGPV
HHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FFRQKRKGIDGHEFEIYKFRSMKVHQEAAGTVTQATKNDSRVTPVGRFLRRTSLDELPQF
HHHHHHCCCCCCEEEEEEEECCHHHHHHCCCHHHCCCCCCCCCHHHHHHHHCCHHHHHHH
INVLKGEMSVVGPRPHALAHDDIYKDLVKGYMFRYRIKPGITGWAQINGYRGETDQIEKM
HHHHCCCHHEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCHHHHHHH
MGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY
HHHHEEEEEEEECCCEEEEHEEEEEHHHCCCCCCCCC
>Mature Secondary Structure
MLSVLARVIDIAMVVAGALLAAALHRGNVWFSDLQRTMVLFDCLLVVVCFPAFGIYQSWR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKRLVGLMGRVAFAWLVVELAGILMSFSFHQSGELSRLWLGYWAIATVTLLAGSKACVHA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
VLRRLRRGGFNLKAVAIVGGTPAARRLIAQMRARPEAGFNPVCVYDEDAAPGDVALDDVR
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHH
IERQFESLVWLVRSRAIRELWLTLPISEERRIHQIVTVFRHDFVNIRFIPDVRSLSFFNQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHH
EVVEVLGVPAINLAASPITDVRILPKFVFDRMFALAALVMLAPLMLVIACLIKATSPGPV
HHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FFRQKRKGIDGHEFEIYKFRSMKVHQEAAGTVTQATKNDSRVTPVGRFLRRTSLDELPQF
HHHHHHCCCCCCEEEEEEEECCHHHHHHCCCHHHCCCCCCCCCHHHHHHHHCCHHHHHHH
INVLKGEMSVVGPRPHALAHDDIYKDLVKGYMFRYRIKPGITGWAQINGYRGETDQIEKM
HHHHCCCHHEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCHHHHHHH
MGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY
HHHHEEEEEEEECCCEEEEHEEEEEHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]