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The map label for this gene is andAa [H]

Identifier: 189352341

GI number: 189352341

Start: 390536

End: 391756

Strand: Direct

Name: andAa [H]

Synonym: BMULJ_03560

Alternate gene names: 189352341

Gene position: 390536-391756 (Clockwise)

Preceding gene: 189352340

Following gene: 189352342

Centisome position: 15.79

GC content: 73.79

Gene sequence:

>1221_bases
ATGTCGGCTGACCCGTTCGTGATCGTCGGCGCCGGCCATGCGGCGCGCCGCACGGCCGAAGCGCTGCGCGCGCGCGATCC
CGGCGTGCGCATCGTGATGATCGGCGCGGAGCGCGAACTGCCGTACGACCGTCCCGCGCTGTCGAAGGACGCGCTGCTCG
GCGACGGCGGCGAACAGCGTGCGTTCGTGCGCGATGCCGGCTGGTACGACGCGCAGCGCATCGCGCTGCGGCTCGGCACG
CGCGTCGACGCGATCGAGCGCGACGCGCAGCGCGTGCGGCTCGACGACGGTTCGAGCCTGCCGTACGCGCGGCTCGTGCT
CGCGACCGGCTCGCGCGTCCGCCCGTTCGCGGGCCCCGTCGACGACGGCGTCGCGCTGCACTACGTGCGTACGGTCGCCG
ACGCGCGTGCGCTGCGCGCGCAGCTCGCGCCCGGCCGTCGCGTCGCGGTGCTCGGCGGCGGCTTCATCGGCCTCGAGGTG
GCGGCGGCCGCGCGGCAGCTCGGCTGCGACGTGACGGTCATCGATCCGGCCGCGCGGCTGCTGCAGCGCGCGCTGCCGGA
AGTGGTCGGCACGTATGCGCGGCAACTGCACGACGGCCGCGGCGTCGCGTTCCGGCTGGCGACGACGCCGCGCGCGGTCC
GCCGCACAGCGGGCGGCGGCGCGATCGTCGACACGGATGGCGGCGACGTGCAGGCCGACGTGGTGGTCGTCGGCATCGGT
GTCGTGCCGAACGTCGAACTGGCGCACGCGGCGGGGCTCGACGTCGACAACGGCATACGCGTCGACGCCGGCTGCCGCAC
GGCCGATCCCGCGATCTTCGCGGCCGGCGAGGTGACGATGCATTTCAATCCGCTGCTCGGCCGCCATGTGCGGATCGAGT
CGTGGCAAGTCGCGGAGAATCAGCCGGCCGTGGCGGCCGCGAACGCGCTCGGCGCCGACGACACGTACGCGGAGCTGCCC
TGGCTGTGGTCCGATCAGTACGACTGCAACCTGCAGATGCTCGGGCTGTTCGGCAGCGAGCACGCGATCGTCGTACGCGG
CGATCCGGCGAGCGGCGCATTCACCGCGTTCGGGCTTGCGCACGACGGCAGGATCGTCGCGGTGGCGGCCGCGAACGCGG
GGCGCGACATCGGCGCATCGCGCAGGCTGATCGCGGCCGGCGCGACGCCCGATCCGGCGCGGCTTGCCGATCCGGCCGTA
AACTTGAAAGCGTTTCTGTAA

Upstream 100 bases:

>100_bases
GGGCTGATCGACATCCGCACCGGTGCGCCGAAGTGTGCACCGATCACCGAGCCGGTGCGCACGTATCCGATCCGGATCGT
CGACGGACAGGTGGAAGTCA

Downstream 100 bases:

>100_bases
CGGGCGTACGGTTGCACGCGCGCATGCGTGGCGGCATGGGCGCGTGCGGTGCATTTGGCGGCAACGGCCGACTCGGTGCA
TATTGGCGGCATCGCGTGCA

Product: ferredoxin-NAD+ reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSADPFVIVGAGHAARRTAEALRARDPGVRIVMIGAERELPYDRPALSKDALLGDGGEQRAFVRDAGWYDAQRIALRLGT
RVDAIERDAQRVRLDDGSSLPYARLVLATGSRVRPFAGPVDDGVALHYVRTVADARALRAQLAPGRRVAVLGGGFIGLEV
AAAARQLGCDVTVIDPAARLLQRALPEVVGTYARQLHDGRGVAFRLATTPRAVRRTAGGGAIVDTDGGDVQADVVVVGIG
VVPNVELAHAAGLDVDNGIRVDAGCRTADPAIFAAGEVTMHFNPLLGRHVRIESWQVAENQPAVAAANALGADDTYAELP
WLWSDQYDCNLQMLGLFGSEHAIVVRGDPASGAFTAFGLAHDGRIVAVAAANAGRDIGASRRLIAAGATPDPARLADPAV
NLKAFL

Sequences:

>Translated_406_residues
MSADPFVIVGAGHAARRTAEALRARDPGVRIVMIGAERELPYDRPALSKDALLGDGGEQRAFVRDAGWYDAQRIALRLGT
RVDAIERDAQRVRLDDGSSLPYARLVLATGSRVRPFAGPVDDGVALHYVRTVADARALRAQLAPGRRVAVLGGGFIGLEV
AAAARQLGCDVTVIDPAARLLQRALPEVVGTYARQLHDGRGVAFRLATTPRAVRRTAGGGAIVDTDGGDVQADVVVVGIG
VVPNVELAHAAGLDVDNGIRVDAGCRTADPAIFAAGEVTMHFNPLLGRHVRIESWQVAENQPAVAAANALGADDTYAELP
WLWSDQYDCNLQMLGLFGSEHAIVVRGDPASGAFTAFGLAHDGRIVAVAAANAGRDIGASRRLIAAGATPDPARLADPAV
NLKAFL
>Mature_405_residues
SADPFVIVGAGHAARRTAEALRARDPGVRIVMIGAERELPYDRPALSKDALLGDGGEQRAFVRDAGWYDAQRIALRLGTR
VDAIERDAQRVRLDDGSSLPYARLVLATGSRVRPFAGPVDDGVALHYVRTVADARALRAQLAPGRRVAVLGGGFIGLEVA
AAARQLGCDVTVIDPAARLLQRALPEVVGTYARQLHDGRGVAFRLATTPRAVRRTAGGGAIVDTDGGDVQADVVVVGIGV
VPNVELAHAAGLDVDNGIRVDAGCRTADPAIFAAGEVTMHFNPLLGRHVRIESWQVAENQPAVAAANALGADDTYAELPW
LWSDQYDCNLQMLGLFGSEHAIVVRGDPASGAFTAFGLAHDGRIVAVAAANAGRDIGASRRLIAAGATPDPARLADPAVN
LKAFL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=390, Percent_Identity=32.0512820512821, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI65787454, Length=390, Percent_Identity=32.0512820512821, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI21389617, Length=390, Percent_Identity=32.0512820512821, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4757732, Length=370, Percent_Identity=29.1891891891892, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI22202629, Length=370, Percent_Identity=29.1891891891892, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI195927006, Length=186, Percent_Identity=28.494623655914, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788892, Length=409, Percent_Identity=37.6528117359413, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789065, Length=319, Percent_Identity=28.5266457680251, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789765, Length=297, Percent_Identity=29.96632996633, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=372, Percent_Identity=30.1075268817204, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32564386, Length=435, Percent_Identity=26.2068965517241, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24639257, Length=405, Percent_Identity=27.1604938271605, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281359715, Length=406, Percent_Identity=27.0935960591133, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281359713, Length=406, Percent_Identity=27.0935960591133, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24639250, Length=406, Percent_Identity=27.0935960591133, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18543267, Length=406, Percent_Identity=27.0935960591133, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24639252, Length=406, Percent_Identity=27.0935960591133, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581020, Length=360, Percent_Identity=26.6666666666667, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28573993, Length=360, Percent_Identity=26.6666666666667, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585130, Length=408, Percent_Identity=22.3039215686275, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42575; Mature: 42444

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADPFVIVGAGHAARRTAEALRARDPGVRIVMIGAERELPYDRPALSKDALLGDGGEQR
CCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCCCCE
AFVRDAGWYDAQRIALRLGTRVDAIERDAQRVRLDDGSSLPYARLVLATGSRVRPFAGPV
EEEECCCCCCHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCC
DDGVALHYVRTVADARALRAQLAPGRRVAVLGGGFIGLEVAAAARQLGCDVTVIDPAARL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEECHHHHH
LQRALPEVVGTYARQLHDGRGVAFRLATTPRAVRRTAGGGAIVDTDGGDVQADVVVVGIG
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCCEEEECCCCCEEEEEEEEEEC
VVPNVELAHAAGLDVDNGIRVDAGCRTADPAIFAAGEVTMHFNPLLGRHVRIESWQVAEN
CCCCCEEHHHCCCCCCCCEEECCCCCCCCCCEEEECEEEEEECCCCCCEEEEEEEEECCC
QPAVAAANALGADDTYAELPWLWSDQYDCNLQMLGLFGSEHAIVVRGDPASGAFTAFGLA
CCCEEEHHCCCCCCCHHHCCEEECCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEEE
HDGRIVAVAAANAGRDIGASRRLIAAGATPDPARLADPAVNLKAFL
CCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHCCCCCCEEECC
>Mature Secondary Structure 
SADPFVIVGAGHAARRTAEALRARDPGVRIVMIGAERELPYDRPALSKDALLGDGGEQR
CCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCCCCE
AFVRDAGWYDAQRIALRLGTRVDAIERDAQRVRLDDGSSLPYARLVLATGSRVRPFAGPV
EEEECCCCCCHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCC
DDGVALHYVRTVADARALRAQLAPGRRVAVLGGGFIGLEVAAAARQLGCDVTVIDPAARL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEECHHHHH
LQRALPEVVGTYARQLHDGRGVAFRLATTPRAVRRTAGGGAIVDTDGGDVQADVVVVGIG
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCCEEEECCCCCEEEEEEEEEEC
VVPNVELAHAAGLDVDNGIRVDAGCRTADPAIFAAGEVTMHFNPLLGRHVRIESWQVAEN
CCCCCEEHHHCCCCCCCCEEECCCCCCCCCCEEEECEEEEEECCCCCCEEEEEEEEECCC
QPAVAAANALGADDTYAELPWLWSDQYDCNLQMLGLFGSEHAIVVRGDPASGAFTAFGLA
CCCEEEHHCCCCCCCHHHCCEEECCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEEE
HDGRIVAVAAANAGRDIGASRRLIAAGATPDPARLADPAVNLKAFL
CCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]