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The map label for this gene is andAc [H]

Identifier: 189352338

GI number: 189352338

Start: 388431

End: 389699

Strand: Direct

Name: andAc [H]

Synonym: BMULJ_03557

Alternate gene names: 189352338

Gene position: 388431-389699 (Clockwise)

Preceding gene: 189352336

Following gene: 189352339

Centisome position: 15.71

GC content: 63.83

Gene sequence:

>1269_bases
ATGGAGCAGACAGAATCGCCCGTCCGGTTCGCCGCGCGCGACGACGCCGACGTGGTTTTCCCGCACGACGACGGCTCGCG
CGTGCCGTACAAGGTGTTCAGCTCGCGCGCGGTGTACGACCGCGAACAGGAACGCATCTTCCGCGGGCCGACCTGGAACT
TCGTCGCGCTGGAAGCGGAAATCCCGAATCCCGGCGACTTCAAGAGCACGTTCGTCGGCGACACGCCCGTCGTCGTCACG
CGCACGGACGACGGCACGCTGGCCGCCTGGGTGAACCGCTGCGCGCATCGCGGCGCGCAGGTCTGCCGCAAGGCGCGCGG
CAACGCGAGTTCGCATACGTGCGTCTATCACCAGTGGAGCTTCGACAACCGCGGCAACCTGCTCGGCGTGCCGTTCCGGC
GCGGCCAGAAGGGTATGACGGGGATGCCGGCCGATTTCGATCCGAAGCAGCACGGGCTGCGCCAGCTGCGCGTCGACAGC
TATCGCGGGCTCGTGTTCGCGACGTTCAGCGACGAGGTCGCGCCGCTGCCCGACTATCTCGGCGAACAGATGCGGCCGTG
GATCGACCGGATCTTCCACAAGCCGATCGAGTATCTCGGCTGTACGCGCCAGTATTCGAAGTCGAACTGGAAGCTCTATC
TCGAGAACGTGAAGGATCCGTATCACGCGAGCATGCTGCATCTGTTCCATACGACGTTCAACATCTTCCGCGTCGGGATG
AAGGCGCGCTCGATTCCGGACGCGAATCACGGGCTGCACAGCATCATCACCGTGACGAAGACGGGCGACGACACGTCGGC
CGCCTACAAGCAGCAGAACATCCGGTCGTTCGACGAGGGCTTTCATCTGGAAGACGAATCGATCCTCGATCTCGTGTCGG
AATACGACGAGGACTGCACGAACCACATTCAGCCGATCTTTCCGCAGCTCGTGATCCAGCAGATCCACAACACGCTGGTC
GCGCGGCAGATCCTGCCGAAGGGGCCCGACAATTTCGAGCTGATCTTCCACTTCTTCGGCTATACCGACGACACGCCCGA
GCTGCGCGCGCTGCGCATCAAGCAGGCGAACCTCGTCGGGCCGGCCGGCTACATCTCGATGGAGGACACCGAAGCGACCG
AGCTCGTGCAGCGCGGCACGGTGCGCGACGGCGACGCGACGTCGGTGATCGAGATGTCGCGCGGCAATCCCGACCAGCAG
GACACGGTGATCACCGAAAGCCTGATCCGCAAGTTCTGGGTCGGCTACCAGAAGCTGATGGGCTATTGA

Upstream 100 bases:

>100_bases
CTGCGCCGCCGCAATCGCACTTTCCGGATAGACGCCGAATATTAGCGTTTCAAAAATCCGCTCCATGGAGTCAACGACAC
GGATCGACAAGGAGTCCGAC

Downstream 100 bases:

>100_bases
ATCGGGAGCGCGCAATGATGGACAACCTGACCGAAGACATGAACACGTGGTTCGCGATCTACATGCTGCAGAACCGCTAC
ATCGGCCACCTCGACAACAA

Product: large terminal subunit of anthranilate dioxygenase

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MEQTESPVRFAARDDADVVFPHDDGSRVPYKVFSSRAVYDREQERIFRGPTWNFVALEAEIPNPGDFKSTFVGDTPVVVT
RTDDGTLAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDNRGNLLGVPFRRGQKGMTGMPADFDPKQHGLRQLRVDS
YRGLVFATFSDEVAPLPDYLGEQMRPWIDRIFHKPIEYLGCTRQYSKSNWKLYLENVKDPYHASMLHLFHTTFNIFRVGM
KARSIPDANHGLHSIITVTKTGDDTSAAYKQQNIRSFDEGFHLEDESILDLVSEYDEDCTNHIQPIFPQLVIQQIHNTLV
ARQILPKGPDNFELIFHFFGYTDDTPELRALRIKQANLVGPAGYISMEDTEATELVQRGTVRDGDATSVIEMSRGNPDQQ
DTVITESLIRKFWVGYQKLMGY

Sequences:

>Translated_422_residues
MEQTESPVRFAARDDADVVFPHDDGSRVPYKVFSSRAVYDREQERIFRGPTWNFVALEAEIPNPGDFKSTFVGDTPVVVT
RTDDGTLAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDNRGNLLGVPFRRGQKGMTGMPADFDPKQHGLRQLRVDS
YRGLVFATFSDEVAPLPDYLGEQMRPWIDRIFHKPIEYLGCTRQYSKSNWKLYLENVKDPYHASMLHLFHTTFNIFRVGM
KARSIPDANHGLHSIITVTKTGDDTSAAYKQQNIRSFDEGFHLEDESILDLVSEYDEDCTNHIQPIFPQLVIQQIHNTLV
ARQILPKGPDNFELIFHFFGYTDDTPELRALRIKQANLVGPAGYISMEDTEATELVQRGTVRDGDATSVIEMSRGNPDQQ
DTVITESLIRKFWVGYQKLMGY
>Mature_422_residues
MEQTESPVRFAARDDADVVFPHDDGSRVPYKVFSSRAVYDREQERIFRGPTWNFVALEAEIPNPGDFKSTFVGDTPVVVT
RTDDGTLAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDNRGNLLGVPFRRGQKGMTGMPADFDPKQHGLRQLRVDS
YRGLVFATFSDEVAPLPDYLGEQMRPWIDRIFHKPIEYLGCTRQYSKSNWKLYLENVKDPYHASMLHLFHTTFNIFRVGM
KARSIPDANHGLHSIITVTKTGDDTSAAYKQQNIRSFDEGFHLEDESILDLVSEYDEDCTNHIQPIFPQLVIQQIHNTLV
ARQILPKGPDNFELIFHFFGYTDDTPELRALRIKQANLVGPAGYISMEDTEATELVQRGTVRDGDATSVIEMSRGNPDQQ
DTVITESLIRKFWVGYQKLMGY

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=407, Percent_Identity=26.2899262899263, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788103, Length=203, Percent_Identity=25.615763546798, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 48133; Mature: 48133

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTESPVRFAARDDADVVFPHDDGSRVPYKVFSSRAVYDREQERIFRGPTWNFVALEAE
CCCCCCCCEEEECCCCCEEEECCCCCCCCHHHHCCCHHHCCHHHHHHCCCCCEEEEEEEC
IPNPGDFKSTFVGDTPVVVTRTDDGTLAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWS
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
FDNRGNLLGVPFRRGQKGMTGMPADFDPKQHGLRQLRVDSYRGLVFATFSDEVAPLPDYL
CCCCCCEEECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
GEQMRPWIDRIFHKPIEYLGCTRQYSKSNWKLYLENVKDPYHASMLHLFHTTFNIFRVGM
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KARSIPDANHGLHSIITVTKTGDDTSAAYKQQNIRSFDEGFHLEDESILDLVSEYDEDCT
HHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NHIQPIFPQLVIQQIHNTLVARQILPKGPDNFELIFHFFGYTDDTPELRALRIKQANLVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCC
PAGYISMEDTEATELVQRGTVRDGDATSVIEMSRGNPDQQDTVITESLIRKFWVGYQKLM
CCCEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
GY
CC
>Mature Secondary Structure
MEQTESPVRFAARDDADVVFPHDDGSRVPYKVFSSRAVYDREQERIFRGPTWNFVALEAE
CCCCCCCCEEEECCCCCEEEECCCCCCCCHHHHCCCHHHCCHHHHHHCCCCCEEEEEEEC
IPNPGDFKSTFVGDTPVVVTRTDDGTLAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWS
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
FDNRGNLLGVPFRRGQKGMTGMPADFDPKQHGLRQLRVDSYRGLVFATFSDEVAPLPDYL
CCCCCCEEECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
GEQMRPWIDRIFHKPIEYLGCTRQYSKSNWKLYLENVKDPYHASMLHLFHTTFNIFRVGM
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KARSIPDANHGLHSIITVTKTGDDTSAAYKQQNIRSFDEGFHLEDESILDLVSEYDEDCT
HHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NHIQPIFPQLVIQQIHNTLVARQILPKGPDNFELIFHFFGYTDDTPELRALRIKQANLVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCC
PAGYISMEDTEATELVQRGTVRDGDATSVIEMSRGNPDQQDTVITESLIRKFWVGYQKLM
CCCEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
GY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]