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The map label for this gene is braC [H]

Identifier: 189352326

GI number: 189352326

Start: 376504

End: 377619

Strand: Direct

Name: braC [H]

Synonym: BMULJ_03545

Alternate gene names: 189352326

Gene position: 376504-377619 (Clockwise)

Preceding gene: 189352325

Following gene: 189352329

Centisome position: 15.22

GC content: 68.46

Gene sequence:

>1116_bases
ATGAAGCTGAAATGCATGATTGCCGTGCTGGCGCTGGGCGGATTCACGATCGGCGCCGCCCACGCGGAACGCGTCGTCAC
GATCGCTTTTTCCGGTCCGCTGACGGGGCCGCAAGCGGTGAGCGGGAAGGACGACGAGAACGGGCTGCGGCTCGCGGTCG
AGACGCTGAACCGCACGCCGATCACGATCGACGGACAGCCAGTCCGATTCCGGATCGTCGCCGAGGACGACCAGGCCGAC
CCGCGCACCGGCGTGCAGGTCGCGCAGCGTCTGCTGGACCGCCAGCCGGCTGCGTTCTTCGGCCCCGAGAATTCGGGCGT
CGCGATTCCGGTGGCACGGCTGACGAACGCCGCGCGCGTGCCGATGCTGACGGTCGCGTCGAACCCGCAGCTGACCGCGC
TCGGCTACGACAACGTGTTTCGCACGGGCGCGAGCGATTCGGTGCTCGGCGCGTCGATGGCCGCGTTTGCGGCCGGGAAG
CTCGGCGCGAAAACGGCCGCGGTGATCGACGACCGCACCGCCTACGGGCAGGGGCTCGCCGAGCAGTTCATCGCGAAGGC
GAAGTCGCTCGGGCTGCAGGTCGTCGCGCGCGAGTTCACGCATTCGCAGGCGACCGATTTCCTCGCGATCCTCGCGACGA
TCAAGGGAAAGAACCCGGACGTGATCTTCTTCGGCGGCTATACGGCGCAGGCCGCGCCGATGGCCAAGCAGATGGTCCAG
CGCGGCGTGCGCGCGCGGCTGCTCGGCGGCGACGGAATCTGCTCGGCCAACATGGGCACCGTCGCGCAGACGGCATCGCG
CAACGTGTACTGCGCGATGAGCGGCTCGGGTCTCGACAGGACCGAGGCCGGCCGGGACTACGTTGCGCGATACCGTGCGC
GCTTCAAGAGCGAGCCGCAGACATACGGCATCACGTACTACGACGGCATGCTGCTGCTCGCGAAAACGATGGCATCCGCG
CAGACGACCGATCCCGCGAAGCTGATCGAGGCGCTGCGCTCGGCCTCGCATGAAGGCGTGGCCGGGACGTACGCGTTCAC
GGTGCGCGGCGAGCTGATGAACGCGCCGACGACCGTGTTCACGTTCGAGGACGGGCAGATGGTGCCGGTGCGCTGA

Upstream 100 bases:

>100_bases
CCGCCCTGGTGTCCTTCACCCGCCGGCCGCCACACGGTTGACACCGCCGCGCCCGGCTCATCCCGGCTCGCGTATCGCGA
GCCATCGTCGAGGAGACTCC

Downstream 100 bases:

>100_bases
CGCGTCAGAGAAAACGGATACGCGCGGCCTCTTCGGGCGCGAGCGTCAGCGCGGCCTTTGCCGCGCCGACCTCGCGCCTG
CCGCCCGGCGGCCTGAGCCG

Product: ABC-type branched-chain amino acid transport system periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLKCMIAVLALGGFTIGAAHAERVVTIAFSGPLTGPQAVSGKDDENGLRLAVETLNRTPITIDGQPVRFRIVAEDDQAD
PRTGVQVAQRLLDRQPAAFFGPENSGVAIPVARLTNAARVPMLTVASNPQLTALGYDNVFRTGASDSVLGASMAAFAAGK
LGAKTAAVIDDRTAYGQGLAEQFIAKAKSLGLQVVAREFTHSQATDFLAILATIKGKNPDVIFFGGYTAQAAPMAKQMVQ
RGVRARLLGGDGICSANMGTVAQTASRNVYCAMSGSGLDRTEAGRDYVARYRARFKSEPQTYGITYYDGMLLLAKTMASA
QTTDPAKLIEALRSASHEGVAGTYAFTVRGELMNAPTTVFTFEDGQMVPVR

Sequences:

>Translated_371_residues
MKLKCMIAVLALGGFTIGAAHAERVVTIAFSGPLTGPQAVSGKDDENGLRLAVETLNRTPITIDGQPVRFRIVAEDDQAD
PRTGVQVAQRLLDRQPAAFFGPENSGVAIPVARLTNAARVPMLTVASNPQLTALGYDNVFRTGASDSVLGASMAAFAAGK
LGAKTAAVIDDRTAYGQGLAEQFIAKAKSLGLQVVAREFTHSQATDFLAILATIKGKNPDVIFFGGYTAQAAPMAKQMVQ
RGVRARLLGGDGICSANMGTVAQTASRNVYCAMSGSGLDRTEAGRDYVARYRARFKSEPQTYGITYYDGMLLLAKTMASA
QTTDPAKLIEALRSASHEGVAGTYAFTVRGELMNAPTTVFTFEDGQMVPVR
>Mature_371_residues
MKLKCMIAVLALGGFTIGAAHAERVVTIAFSGPLTGPQAVSGKDDENGLRLAVETLNRTPITIDGQPVRFRIVAEDDQAD
PRTGVQVAQRLLDRQPAAFFGPENSGVAIPVARLTNAARVPMLTVASNPQLTALGYDNVFRTGASDSVLGASMAAFAAGK
LGAKTAAVIDDRTAYGQGLAEQFIAKAKSLGLQVVAREFTHSQATDFLAILATIKGKNPDVIFFGGYTAQAAPMAKQMVQ
RGVRARLLGGDGICSANMGTVAQTASRNVYCAMSGSGLDRTEAGRDYVARYRARFKSEPQTYGITYYDGMLLLAKTMASA
QTTDPAKLIEALRSASHEGVAGTYAFTVRGELMNAPTTVFTFEDGQMVPVR

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=340, Percent_Identity=25.2941176470588, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994941, Length=339, Percent_Identity=24.4837758112094, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39210; Mature: 39210

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKCMIAVLALGGFTIGAAHAERVVTIAFSGPLTGPQAVSGKDDENGLRLAVETLNRTP
CCHHHEEHHHHHCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ITIDGQPVRFRIVAEDDQADPRTGVQVAQRLLDRQPAAFFGPENSGVAIPVARLTNAARV
EEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHCCCCCC
PMLTVASNPQLTALGYDNVFRTGASDSVLGASMAAFAAGKLGAKTAAVIDDRTAYGQGLA
CEEEEECCCCEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCHHH
EQFIAKAKSLGLQVVAREFTHSQATDFLAILATIKGKNPDVIFFGGYTAQAAPMAKQMVQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
RGVRARLLGGDGICSANMGTVAQTASRNVYCAMSGSGLDRTEAGRDYVARYRARFKSEPQ
CCCEEEEECCCCEECCCCCHHHHHCCCCEEEEECCCCCCCHHCCHHHHHHHHHHHCCCCC
TYGITYYDGMLLLAKTMASAQTTDPAKLIEALRSASHEGVAGTYAFTVRGELMNAPTTVF
EEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCEEEE
TFEDGQMVPVR
EECCCCEECCC
>Mature Secondary Structure
MKLKCMIAVLALGGFTIGAAHAERVVTIAFSGPLTGPQAVSGKDDENGLRLAVETLNRTP
CCHHHEEHHHHHCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ITIDGQPVRFRIVAEDDQADPRTGVQVAQRLLDRQPAAFFGPENSGVAIPVARLTNAARV
EEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHCCCCCC
PMLTVASNPQLTALGYDNVFRTGASDSVLGASMAAFAAGKLGAKTAAVIDDRTAYGQGLA
CEEEEECCCCEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCHHH
EQFIAKAKSLGLQVVAREFTHSQATDFLAILATIKGKNPDVIFFGGYTAQAAPMAKQMVQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
RGVRARLLGGDGICSANMGTVAQTASRNVYCAMSGSGLDRTEAGRDYVARYRARFKSEPQ
CCCEEEEECCCCEECCCCCHHHHHCCCCEEEEECCCCCCCHHCCHHHHHHHHHHHCCCCC
TYGITYYDGMLLLAKTMASAQTTDPAKLIEALRSASHEGVAGTYAFTVRGELMNAPTTVF
EEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCEEEE
TFEDGQMVPVR
EECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]