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The map label for this gene is fixJ [H]

Identifier: 189352210

GI number: 189352210

Start: 250135

End: 250767

Strand: Direct

Name: fixJ [H]

Synonym: BMULJ_03428

Alternate gene names: 189352210

Gene position: 250135-250767 (Clockwise)

Preceding gene: 189352208

Following gene: 189352216

Centisome position: 10.11

GC content: 67.3

Gene sequence:

>633_bases
ATGACGTCACCCGCTCCGATCGTATATATCGTCGACGACGACGGCGGCATGCGGACCTCGCTCGCATGGCTGCTCGAATC
GGTCGGCATCGCGTCGGAGGGATTCGCGAACGCGGCGGATTTTCTCGCGCGCTTCGATGCGAACCTGCCCGCCTGCCTCG
TGCTCGACGTGCGGATGCCGGAAAAAAGCGGGTTCGACGTGCAGGCCGAGCTGAACGCGCGCGGCGCGACGCTGCCGGTG
ATCTTCGTCAGCGGGCACGGCGACATTCCGATGTCGGTGCGCGCGCTGCAGAACGGCGCGATCGATTTCGTCGAGAAGCC
GTACAACTCGCAGCAGATGCTCGAACGCGTGCAGCGCGCGCTGCGCCTCGCGCAGCAGCGGCATGTGGCCGACCAGCGTC
ACCGCGAACTGCGCGAGCGGCTCGCGGCGCTGACCGCGCGCGAGAAGGAGGTGCTGCAGGGCGTCGTCGACGGCAAGGGC
AGCAAGCAGATCGCATCGGATCTGTCGATCAGCGTGAAGACCGTCGACGTGCATCGCGCGAGCATCAAGGAGAAGCTCGG
CGCGACGTCGATCGCCGCACTCGTGCGCGACGTGATGGTCGTCTGGGGCGACGAACGCGAGCGGCCGCGATAG

Upstream 100 bases:

>100_bases
CGCTATGATGTGCCGCACGTGTGTGCCGCGCCCGACGTGCGCACGTGCGCCATCGCCGATGTACGGCGCACAATCCTCCC
CAATCATGCGAGACACCCCG

Downstream 100 bases:

>100_bases
CGGCCGACGAAACGGTTGCGGCCCGCGGCGCGGGCTGCGTACCTCGCGCGCTCAGCGCATGTACAAGCCGCCGTTGATGT
CCCAGCACGCGCCGTTCGCG

Product: LuxR family two component transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTSPAPIVYIVDDDGGMRTSLAWLLESVGIASEGFANAADFLARFDANLPACLVLDVRMPEKSGFDVQAELNARGATLPV
IFVSGHGDIPMSVRALQNGAIDFVEKPYNSQQMLERVQRALRLAQQRHVADQRHRELRERLAALTAREKEVLQGVVDGKG
SKQIASDLSISVKTVDVHRASIKEKLGATSIAALVRDVMVVWGDERERPR

Sequences:

>Translated_210_residues
MTSPAPIVYIVDDDGGMRTSLAWLLESVGIASEGFANAADFLARFDANLPACLVLDVRMPEKSGFDVQAELNARGATLPV
IFVSGHGDIPMSVRALQNGAIDFVEKPYNSQQMLERVQRALRLAQQRHVADQRHRELRERLAALTAREKEVLQGVVDGKG
SKQIASDLSISVKTVDVHRASIKEKLGATSIAALVRDVMVVWGDERERPR
>Mature_209_residues
TSPAPIVYIVDDDGGMRTSLAWLLESVGIASEGFANAADFLARFDANLPACLVLDVRMPEKSGFDVQAELNARGATLPVI
FVSGHGDIPMSVRALQNGAIDFVEKPYNSQQMLERVQRALRLAQQRHVADQRHRELRERLAALTAREKEVLQGVVDGKGS
KQIASDLSISVKTVDVHRASIKEKLGATSIAALVRDVMVVWGDERERPR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=33.0578512396694, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1790860, Length=205, Percent_Identity=28.2926829268293, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=29.2307692307692, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786747, Length=199, Percent_Identity=25.6281407035176, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=28.2828282828283, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23043; Mature: 22911

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPAPIVYIVDDDGGMRTSLAWLLESVGIASEGFANAADFLARFDANLPACLVLDVRMP
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECC
EKSGFDVQAELNARGATLPVIFVSGHGDIPMSVRALQNGAIDFVEKPYNSQQMLERVQRA
CCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
LRLAQQRHVADQRHRELRERLAALTAREKEVLQGVVDGKGSKQIASDLSISVKTVDVHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEEHHHHH
SIKEKLGATSIAALVRDVMVVWGDERERPR
HHHHHHCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSPAPIVYIVDDDGGMRTSLAWLLESVGIASEGFANAADFLARFDANLPACLVLDVRMP
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECC
EKSGFDVQAELNARGATLPVIFVSGHGDIPMSVRALQNGAIDFVEKPYNSQQMLERVQRA
CCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
LRLAQQRHVADQRHRELRERLAALTAREKEVLQGVVDGKGSKQIASDLSISVKTVDVHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEEHHHHH
SIKEKLGATSIAALVRDVMVVWGDERERPR
HHHHHHCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]