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The map label for this gene is livK [C]

Identifier: 189352147

GI number: 189352147

Start: 173562

End: 174713

Strand: Direct

Name: livK [C]

Synonym: BMULJ_03365

Alternate gene names: 189352147

Gene position: 173562-174713 (Clockwise)

Preceding gene: 189352146

Following gene: 189352148

Centisome position: 7.02

GC content: 66.06

Gene sequence:

>1152_bases
ATGACGTTCCGCCTCCGCCTGCTTTCCGCCGCCGCGCTGACCGTTGCGAACCTCGCGCTCCCGACGGGCGCGGCCCATGC
GCAGGAAGCGATCGTCAAGGTCGGCGTGGCCGTGCCGTTGACGGGCGGCGGCGCCGCATACGGCAAGGACATCGAGAACG
GCGTGCGGATGGCCGTCGATGAAGCGAACGCCGCGCGCACGACGATCGGCGGCAAGCCGGTGAAGTTCGTGATCGCGTCG
CAGGACGATCACAGCGATCCGCGGATCGGCGTGCAGGCCGCGCAGCAACTTGCGGACGGGCAGGTCGCGGTCGTGATCGG
CCACTTCAACTCGGGCACGACGCTGCCCGCCTCGAAGATCTACGCGAAGGCCGGCATTCCGATGATCACGCCGTCGGCGA
CCAATCCGGACATCACGCGCGCGGGCCTCGGCACCGTCTATCGCGTGATCGCGACCGACACGCAGAACGCGGGCAACGCG
GGCGCGTATGCGGCAAGCATCACGAAGGCGAAGCGCATCGCGATCATCGACGATCGCACCGCGTTCGGTCAGGGCGAGGC
CGACGAGTTCGAGAAGGCCGTGAAGGCGAACGGCGGCACGATCGTCGCGCGCGAATTCACGAACGACAAGGCCGTCGACT
TCAGCGCGCAACTCACGAAGATCAAGAGCGTGAATGCCGATCTGGTGTTTTTCGGCGGACTGGACGCGCAGTCCGCCATG
CTGGTGAAGCGGATGCGCCAGCTCGGCATTCGCGCGCAGTTCCTCGCGGGCGGCGGCGTGATGAACGCCAACTTCATCAA
GCTGGCCGGCAGTGCGGCGGAAGGCGCGGCCGTGTGGGAGTACGGACAGCCGCTGTCGCGACTCGCGAAGGGCAAGCTGT
TCGAGACGAAATTCAAGCAGAAGTACGGCGTCGACATGCTCGCGTATGCGCCGTTCGCGTACGACGCGACCTGGATCGCG
ATCAACGCGATGCAGAAGGCGAACTCGACGAAGCCGGCCGATTTCAATGCAGCGCTCAAGCGCACGCAGTACGACGGCAT
CACCGGCACGATCGCGTTCACCGAGACGGGCGACCTCAGGAATCCGAGCTCGACGCTGTACGAAGTGAAGAACGCCGCGT
GGCAGCCCGTCACGACGAAGTCGGCAGACTGA

Upstream 100 bases:

>100_bases
CCGGAGACCGGACCGCAGACCGCTCGGTCGACGCGCGGGAGGCGCGCCGACGCGCAGCCGCGCGTCGCATTTCGACGCTC
AGAACAAGAGGACTGCCCCG

Downstream 100 bases:

>100_bases
GCGCGCCGCGCGCACCGCTTTCGCCGCGCCGACGGACATGCTGAAATACCGTCGGCGACCGGGCGCGGCCGCGAACTCAA
CCTCTTTCGGGCGCGGCCGG

Product: branched-chain amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTFRLRLLSAAALTVANLALPTGAAHAQEAIVKVGVAVPLTGGGAAYGKDIENGVRMAVDEANAARTTIGGKPVKFVIAS
QDDHSDPRIGVQAAQQLADGQVAVVIGHFNSGTTLPASKIYAKAGIPMITPSATNPDITRAGLGTVYRVIATDTQNAGNA
GAYAASITKAKRIAIIDDRTAFGQGEADEFEKAVKANGGTIVAREFTNDKAVDFSAQLTKIKSVNADLVFFGGLDAQSAM
LVKRMRQLGIRAQFLAGGGVMNANFIKLAGSAAEGAAVWEYGQPLSRLAKGKLFETKFKQKYGVDMLAYAPFAYDATWIA
INAMQKANSTKPADFNAALKRTQYDGITGTIAFTETGDLRNPSSTLYEVKNAAWQPVTTKSAD

Sequences:

>Translated_383_residues
MTFRLRLLSAAALTVANLALPTGAAHAQEAIVKVGVAVPLTGGGAAYGKDIENGVRMAVDEANAARTTIGGKPVKFVIAS
QDDHSDPRIGVQAAQQLADGQVAVVIGHFNSGTTLPASKIYAKAGIPMITPSATNPDITRAGLGTVYRVIATDTQNAGNA
GAYAASITKAKRIAIIDDRTAFGQGEADEFEKAVKANGGTIVAREFTNDKAVDFSAQLTKIKSVNADLVFFGGLDAQSAM
LVKRMRQLGIRAQFLAGGGVMNANFIKLAGSAAEGAAVWEYGQPLSRLAKGKLFETKFKQKYGVDMLAYAPFAYDATWIA
INAMQKANSTKPADFNAALKRTQYDGITGTIAFTETGDLRNPSSTLYEVKNAAWQPVTTKSAD
>Mature_382_residues
TFRLRLLSAAALTVANLALPTGAAHAQEAIVKVGVAVPLTGGGAAYGKDIENGVRMAVDEANAARTTIGGKPVKFVIASQ
DDHSDPRIGVQAAQQLADGQVAVVIGHFNSGTTLPASKIYAKAGIPMITPSATNPDITRAGLGTVYRVIATDTQNAGNAG
AYAASITKAKRIAIIDDRTAFGQGEADEFEKAVKANGGTIVAREFTNDKAVDFSAQLTKIKSVNADLVFFGGLDAQSAML
VKRMRQLGIRAQFLAGGGVMNANFIKLAGSAAEGAAVWEYGQPLSRLAKGKLFETKFKQKYGVDMLAYAPFAYDATWIAI
NAMQKANSTKPADFNAALKRTQYDGITGTIAFTETGDLRNPSSTLYEVKNAAWQPVTTKSAD

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=333, Percent_Identity=31.5315315315315, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994941, Length=362, Percent_Identity=29.8342541436464, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40213; Mature: 40082

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRLRLLSAAALTVANLALPTGAAHAQEAIVKVGVAVPLTGGGAAYGKDIENGVRMAVD
CEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHCCCEEEEE
EANAARTTIGGKPVKFVIASQDDHSDPRIGVQAAQQLADGQVAVVIGHFNSGTTLPASKI
CCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
YAKAGIPMITPSATNPDITRAGLGTVYRVIATDTQNAGNAGAYAASITKAKRIAIIDDRT
HHHCCCCEECCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCEEEEEEECCC
AFGQGEADEFEKAVKANGGTIVAREFTNDKAVDFSAQLTKIKSVNADLVFFGGLDAQSAM
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHCCCCCCEEEEECCCHHHHH
LVKRMRQLGIRAQFLAGGGVMNANFIKLAGSAAEGAAVWEYGQPLSRLAKGKLFETKFKQ
HHHHHHHHCCEEEEEECCCEECCCEEEEECCCCCCCEEECCCCHHHHHHCCCHHHHHHHH
KYGVDMLAYAPFAYDATWIAINAMQKANSTKPADFNAALKRTQYDGITGTIAFTETGDLR
HHCCCEEEECCCCCCCEEEEEEEHHHCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCC
NPSSTLYEVKNAAWQPVTTKSAD
CCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TFRLRLLSAAALTVANLALPTGAAHAQEAIVKVGVAVPLTGGGAAYGKDIENGVRMAVD
EEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHCCCEEEEE
EANAARTTIGGKPVKFVIASQDDHSDPRIGVQAAQQLADGQVAVVIGHFNSGTTLPASKI
CCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
YAKAGIPMITPSATNPDITRAGLGTVYRVIATDTQNAGNAGAYAASITKAKRIAIIDDRT
HHHCCCCEECCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCEEEEEEECCC
AFGQGEADEFEKAVKANGGTIVAREFTNDKAVDFSAQLTKIKSVNADLVFFGGLDAQSAM
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHCCCCCCEEEEECCCHHHHH
LVKRMRQLGIRAQFLAGGGVMNANFIKLAGSAAEGAAVWEYGQPLSRLAKGKLFETKFKQ
HHHHHHHHCCEEEEEECCCEECCCEEEEECCCCCCCEEECCCCHHHHHHCCCHHHHHHHH
KYGVDMLAYAPFAYDATWIAINAMQKANSTKPADFNAALKRTQYDGITGTIAFTETGDLR
HHCCCEEEECCCCCCCEEEEEEEHHHCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCC
NPSSTLYEVKNAAWQPVTTKSAD
CCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA