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The map label for this gene is trmE [H]

Identifier: 189184774

GI number: 189184774

Start: 1900807

End: 1902150

Strand: Reverse

Name: trmE [H]

Synonym: OTT_1867

Alternate gene names: 189184774

Gene position: 1902150-1900807 (Counterclockwise)

Preceding gene: 189184777

Following gene: 189184773

Centisome position: 94.68

GC content: 32.89

Gene sequence:

>1344_bases
ATGACTAATAAAACTATATTTGCACAGTCCTCTGCTAAAGGCAAAGCAGGCGTTGCTGTTTTTAGGATTTCTGGTGGTTT
GTCATTATTGATTGTTGAAAGATTATGTGGTAAATTTAATATAGTACCAAGGAAAGTTTATTATCGTACTATTCGCTGTT
ATATTACATCGCAAGTCATTGACAAGGCATTAATAGTGTACTTCCAAGGTGAGTACAGCTTTACAGGAGAAGATGTAGTA
GAAATACATACGCATGGTAGTGTTGCTGTCGCAAAAATGTTAACTAGGTCTATACTGGAATGTGATGGTGTTAGATTGGC
TGAACCTGGTGAATTTGCAAAGCGAGCTTTCTTAAATGGTAAAATGGATCTAACTATGGCAGAAGGATTAGTGGATTTGA
TTGAATCAGAAACACTAATGCAGCATAAGCAAGCAATCAGGCAAATGGGAGGTGAGCTTGAGAAATTATATAGCCACTGG
CGTGGAATGTTAATAAAGATACTAAGTTTTGTTGAAGGATATATTGATTTTCCAGATGAAGAAATACCGCAGTCAGTATT
AAGAGAAGCAAAATCAATTATTAATAATCTAACTCAAGAAATTTCTAATCATTTAGGAGATGCTCGTAAAGGTGAAGTTC
TTAGACATGGGATAGTATTAGCAATTACTGGTGAAACTAATACTGGAAAATCTAGTTTATTAAACTATTTAACTATGAGG
GAAGCAGCTATTGTTTCAGATATTCCAGGAACTACTAGAGATGTTATTGAAGCTCATCTTGATATTGGAGGATATCCAAT
AATAGTACGTGATACTGCTGGTATTAGAGAAAGTGATGATCCAATAGAACAAGAAGGTATTAAAAGGTCATTACTAGCAT
TTAAAAATTCTGATATTAGAATTTTAATGATTGATGCTACAAATATGAACAGCGTTAATCAAACTATTGCTCATTTGTTG
AATGATGTTTATACAATCTTAGTAATTAATAAGATTGATCTAGTAAATTATAGGTATGACGGTAGCAGGTTACCATGCGA
TAAACCAATAGTTGCAATCTCATTGCTAAAACAGGTTGGATTAGATAGATTAATGGCAGAAATAGTAAGCTATGCAGAAA
AAATAGCAGATCCAGGAAATGTTCCTGCCATTACTAGAGAAAGATATCGTAATAGTTTAAATCAGGCACTAGAATTATTA
CAGTTAGTAAATTTAGAAAATGATTTGGTGCTTGCTGCTGAAGATTTAAGAATGGCAATTAGATATCTAGAGCATATTAC
TGGAAAGATTAAAATAGATGATATTTTAGCAGAAATTTTTGCTAGCTTCTGTATAGGTAAATAA

Upstream 100 bases:

>100_bases
CTAAATCTTGATTATTCATATATATAAAATATAATTTTAAAAATAGCAGATAGTTTATGTTATATTATAGCTTTCAGCAA
GAATAAAGTAATATCTAAAA

Downstream 100 bases:

>100_bases
GTTTAATTATATTAATATTTGTATTATGCAACTTAATTCTCAACTTGAAAAAATTATAACTGAGTGGCTTAACTATCTTA
AAAACTATAAAAATTATTCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTNKTIFAQSSAKGKAGVAVFRISGGLSLLIVERLCGKFNIVPRKVYYRTIRCYITSQVIDKALIVYFQGEYSFTGEDVV
EIHTHGSVAVAKMLTRSILECDGVRLAEPGEFAKRAFLNGKMDLTMAEGLVDLIESETLMQHKQAIRQMGGELEKLYSHW
RGMLIKILSFVEGYIDFPDEEIPQSVLREAKSIINNLTQEISNHLGDARKGEVLRHGIVLAITGETNTGKSSLLNYLTMR
EAAIVSDIPGTTRDVIEAHLDIGGYPIIVRDTAGIRESDDPIEQEGIKRSLLAFKNSDIRILMIDATNMNSVNQTIAHLL
NDVYTILVINKIDLVNYRYDGSRLPCDKPIVAISLLKQVGLDRLMAEIVSYAEKIADPGNVPAITRERYRNSLNQALELL
QLVNLENDLVLAAEDLRMAIRYLEHITGKIKIDDILAEIFASFCIGK

Sequences:

>Translated_447_residues
MTNKTIFAQSSAKGKAGVAVFRISGGLSLLIVERLCGKFNIVPRKVYYRTIRCYITSQVIDKALIVYFQGEYSFTGEDVV
EIHTHGSVAVAKMLTRSILECDGVRLAEPGEFAKRAFLNGKMDLTMAEGLVDLIESETLMQHKQAIRQMGGELEKLYSHW
RGMLIKILSFVEGYIDFPDEEIPQSVLREAKSIINNLTQEISNHLGDARKGEVLRHGIVLAITGETNTGKSSLLNYLTMR
EAAIVSDIPGTTRDVIEAHLDIGGYPIIVRDTAGIRESDDPIEQEGIKRSLLAFKNSDIRILMIDATNMNSVNQTIAHLL
NDVYTILVINKIDLVNYRYDGSRLPCDKPIVAISLLKQVGLDRLMAEIVSYAEKIADPGNVPAITRERYRNSLNQALELL
QLVNLENDLVLAAEDLRMAIRYLEHITGKIKIDDILAEIFASFCIGK
>Mature_446_residues
TNKTIFAQSSAKGKAGVAVFRISGGLSLLIVERLCGKFNIVPRKVYYRTIRCYITSQVIDKALIVYFQGEYSFTGEDVVE
IHTHGSVAVAKMLTRSILECDGVRLAEPGEFAKRAFLNGKMDLTMAEGLVDLIESETLMQHKQAIRQMGGELEKLYSHWR
GMLIKILSFVEGYIDFPDEEIPQSVLREAKSIINNLTQEISNHLGDARKGEVLRHGIVLAITGETNTGKSSLLNYLTMRE
AAIVSDIPGTTRDVIEAHLDIGGYPIIVRDTAGIRESDDPIEQEGIKRSLLAFKNSDIRILMIDATNMNSVNQTIAHLLN
DVYTILVINKIDLVNYRYDGSRLPCDKPIVAISLLKQVGLDRLMAEIVSYAEKIADPGNVPAITRERYRNSLNQALELLQ
LVNLENDLVLAAEDLRMAIRYLEHITGKIKIDDILAEIFASFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=462, Percent_Identity=38.3116883116883, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI193082993, Length=450, Percent_Identity=38.6666666666667, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=35.82995951417, Blast_Score=285, Evalue=7e-77,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=36.2255965292842, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI87082120, Length=118, Percent_Identity=38.135593220339, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=453, Percent_Identity=32.2295805739514, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=35.1738241308793, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=36.7864693446089, Blast_Score=285, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49843; Mature: 49711

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKTIFAQSSAKGKAGVAVFRISGGLSLLIVERLCGKFNIVPRKVYYRTIRCYITSQVI
CCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
DKALIVYFQGEYSFTGEDVVEIHTHGSVAVAKMLTRSILECDGVRLAEPGEFAKRAFLNG
HCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHCC
KMDLTMAEGLVDLIESETLMQHKQAIRQMGGELEKLYSHWRGMLIKILSFVEGYIDFPDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EIPQSVLREAKSIINNLTQEISNHLGDARKGEVLRHGIVLAITGETNTGKSSLLNYLTMR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHHHHHH
EAAIVSDIPGTTRDVIEAHLDIGGYPIIVRDTAGIRESDDPIEQEGIKRSLLAFKNSDIR
HHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
ILMIDATNMNSVNQTIAHLLNDVYTILVINKIDLVNYRYDGSRLPCDKPIVAISLLKQVG
EEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHC
LDRLMAEIVSYAEKIADPGNVPAITRERYRNSLNQALELLQLVNLENDLVLAAEDLRMAI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
RYLEHITGKIKIDDILAEIFASFCIGK
HHHHHHCCCEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNKTIFAQSSAKGKAGVAVFRISGGLSLLIVERLCGKFNIVPRKVYYRTIRCYITSQVI
CCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
DKALIVYFQGEYSFTGEDVVEIHTHGSVAVAKMLTRSILECDGVRLAEPGEFAKRAFLNG
HCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHCC
KMDLTMAEGLVDLIESETLMQHKQAIRQMGGELEKLYSHWRGMLIKILSFVEGYIDFPDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EIPQSVLREAKSIINNLTQEISNHLGDARKGEVLRHGIVLAITGETNTGKSSLLNYLTMR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHHHHHH
EAAIVSDIPGTTRDVIEAHLDIGGYPIIVRDTAGIRESDDPIEQEGIKRSLLAFKNSDIR
HHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
ILMIDATNMNSVNQTIAHLLNDVYTILVINKIDLVNYRYDGSRLPCDKPIVAISLLKQVG
EEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHC
LDRLMAEIVSYAEKIADPGNVPAITRERYRNSLNQALELLQLVNLENDLVLAAEDLRMAI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
RYLEHITGKIKIDDILAEIFASFCIGK
HHHHHHCCCEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA