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The map label for this gene is dksA [H]

Identifier: 189184772

GI number: 189184772

Start: 1899377

End: 1899808

Strand: Reverse

Name: dksA [H]

Synonym: OTT_1865

Alternate gene names: 189184772

Gene position: 1899808-1899377 (Counterclockwise)

Preceding gene: 189184773

Following gene: 189184771

Centisome position: 94.57

GC content: 30.09

Gene sequence:

>432_bases
ATGAGTAAAATAATATTACCTAAAGGATATAAACCTTCTGAAGAAGAAGAGTATATGAGTCCAAACCAGCTTGAATACTT
TAAGCAAAAGCTTGAGAAATGGAAAGAAGAATTAATTCAAGAATCTTTAGACACTATTGATCATTTAAAAGATGGTCATC
TTAAAGAGCCTGATTTAAACTATCAAGCTTCTAGAGAAGCAGATACTGCTATTGAACTTAAAACAAGAAGTAGGTACAAA
AAGTTAATTGATAAAATTGAAACTGCATTAAAGAAAATTGAATTAAAACAATATGGTTATTGTGAAGAAAGCGGAGAAAA
AATAGGATTAAAAAGGCTTGAAGCTCGCCCAATAGCAACATTATGTATTGAATCACAGGAACGCCATGAAAAATATGAAA
AGCAGCATAATGATGATTATGATGCAGAATAA

Upstream 100 bases:

>100_bases
GATTTTTTAACTTCAGTGTATAATAAATCACACCCATTAGTTAGAGAACAAAATAATAAAACTTAATTAAGTGGTTAATT
AAAAAATAATATAGTGCATT

Downstream 100 bases:

>100_bases
ACTTAAAAAGTTTATTTAATAATATAATGGCAATCATACCAACTAGCTATAGATGAAAAAATAATTAATAAATTGGAGAT
TTGCAGTGTTTATTGAGAAA

Product: dnaK suppressor protein homolog

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSKIILPKGYKPSEEEEYMSPNQLEYFKQKLEKWKEELIQESLDTIDHLKDGHLKEPDLNYQASREADTAIELKTRSRYK
KLIDKIETALKKIELKQYGYCEESGEKIGLKRLEARPIATLCIESQERHEKYEKQHNDDYDAE

Sequences:

>Translated_143_residues
MSKIILPKGYKPSEEEEYMSPNQLEYFKQKLEKWKEELIQESLDTIDHLKDGHLKEPDLNYQASREADTAIELKTRSRYK
KLIDKIETALKKIELKQYGYCEESGEKIGLKRLEARPIATLCIESQERHEKYEKQHNDDYDAE
>Mature_142_residues
SKIILPKGYKPSEEEEYMSPNQLEYFKQKLEKWKEELIQESLDTIDHLKDGHLKEPDLNYQASREADTAIELKTRSRYKK
LIDKIETALKKIELKQYGYCEESGEKIGLKRLEARPIATLCIESQERHEKYEKQHNDDYDAE

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=135, Percent_Identity=42.962962962963, Blast_Score=107, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 16995; Mature: 16864

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIILPKGYKPSEEEEYMSPNQLEYFKQKLEKWKEELIQESLDTIDHLKDGHLKEPDLN
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
YQASREADTAIELKTRSRYKKLIDKIETALKKIELKQYGYCEESGEKIGLKRLEARPIAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHCCCHHH
LCIESQERHEKYEKQHNDDYDAE
HHHCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKIILPKGYKPSEEEEYMSPNQLEYFKQKLEKWKEELIQESLDTIDHLKDGHLKEPDLN
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
YQASREADTAIELKTRSRYKKLIDKIETALKKIELKQYGYCEESGEKIGLKRLEARPIAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHCCCHHH
LCIESQERHEKYEKQHNDDYDAE
HHHCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555902 [H]