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The map label for this gene is aspS [H]

Identifier: 189184699

GI number: 189184699

Start: 1817410

End: 1819203

Strand: Reverse

Name: aspS [H]

Synonym: OTT_1792

Alternate gene names: 189184699

Gene position: 1819203-1817410 (Counterclockwise)

Preceding gene: 189184700

Following gene: 189184698

Centisome position: 90.55

GC content: 34.0

Gene sequence:

>1794_bases
ATGCATAAATACAGAACACATACATGCGGGGAGCTTAATTTAGCCCACGTTGGGCTGCAAGTTAAGTTATCAGGATGGTT
ATATAGAAGGCGTGATCATGGTGGTTTATTGTTCATTGATTTAAGAGATCATTATGGTATTATTCAACTTGTATTCAATG
ATGATTGTTCTATTATCCAAGCAGAAATTAGTAAAATAAAATTTGAAACTGTGATTACTGTGGAGGGTACTGTTATTGCT
AGAAGTAATGAAACTATTAACAAGAATATTAGTACTGGCGATGTTGAAGTAGTAGTAAGCAGTTATGTTGTTGAATCATT
TGCCGAAGAGTTACCTTTACAGATTAATGGTGAATATGAAGCTCCAGAAGAAACTAGGTTAAAGTACCGCTTTCTTGACC
TGCGTGGAGCAAGATTACATCAGAATATTGTCTTAAGGTCAAAAGTGATTCAAGAGCTAAGAAATCAGATGCTTAGTAAT
GGTTTTATTGAGTTTCAAACCCCTATACTTACAGCAAGCTCTCCTGAGGGAGCTCGAGATTTTTTGGTACCAAGTAGATT
GCATCCTGGTAAATTTTATGCTTTACCACAAGCTCCTCAACAGTTTAAGCAGTTAATAATGATGTCAGGATTTGATAAAT
ATTTTCAAATTGCTCCGTGTTTTAGGGATGAGGATGCAAGAGCTGATCGATCTCCAGGAGAGTTTTATCAATTGGATATT
GAAATGGCTTTTGTTACTCAAGAAGATGTTTTTGGGCTTGTTGAACCAGTTCTTTACAATACATTTAAAAAATTTTCCAA
TTACTCTATTACTGATATACCATTTCCACGTATTACTTATGATGAGTCAATGCTAAGGTATGGTTCAGATAAGCCAGATT
TACGTAATCCTATTCAGATTAGTGATGTAACTGATATTTTTCAATGCTCTGAGTTTACTACCTTAAAAGATAGTATTAAT
TGTGGGGCTGTTGTAAGAGCAATTCCAGCACCAATGTCAGCTAATAGGCCTCGCAGCTTTTTTGATAAAATGATTGAATA
TGCTAAACATCTTGGTGCTAGTGGGCTAGCGTACATTCAGTTTACTAATGACGGTGTTAAGGGATCTATAGCTAAATTTT
TTGATGAAGCATTATTAGCTCGAATTAAAAAACTGGTTAAATGCCAGGATGGAGATGCAATATTTTTTATTTGCGATAAA
GAAGATGATGCTACTAAAGTTGCAGCTAAAATTAGAGCTAAAGTAGGAGAAGAGCTAAACATAATAAAAACAGATTGTTA
TAAATTTTGTTGGGTTATTGATTTTCCTTTTTATCAGTTAGATTCTGAAGTAAATAAAATAACATTTAGTCATAATCCTT
TTTCGATGCCACAAGGAGGAATAGAAGCTTTTAATAAAGCTAAAACTGTTGAGGATTTACTTAGTATTAAAGCGTTTCAA
TATGATATAGTGTGTAATGGAGTTGAATTATCTAGTGGGGCTATTAGAAATCATAAGCCAGATTTAATGTATAAGGCATT
TGCTATAGCTGGTTATTCAAAAGATGAGGTTGATAAGCAATTTGGAGCAATGATTAGGGCGTTTAGCTATGGAGCTCCAC
CTCATGGAGGAATTGCTCCTGGAATTGATCGAATACTAATGCTATTAACTAATTCTGTTAATATTCGTGAAGTAATAGCA
TTTCCAATGAATCAACAGGCTGAAGATTTGTTAATGGGGGCTCCTTCAGCTGTAACTGAGAAACAATTACAGGAGTTACA
GCTAAAGTTAATATCTAAAGAAAGAATTAAATAA

Upstream 100 bases:

>100_bases
ATATCTTCTAGTTCTTGTATACAAAATTGATGAAATGTATTTTTATATCATTTAGCATCTAAAATCATTTTAAGTATTAA
AATATAACTAATAATTTAAT

Downstream 100 bases:

>100_bases
ATTATTTTAATAAAAGAATATAGATATGAAGATAATGAAAATAACAAAATTTTATATTTTATTAGTATTGATTTTACTAC
TAATACCAAGCGCTTCTTTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MHKYRTHTCGELNLAHVGLQVKLSGWLYRRRDHGGLLFIDLRDHYGIIQLVFNDDCSIIQAEISKIKFETVITVEGTVIA
RSNETINKNISTGDVEVVVSSYVVESFAEELPLQINGEYEAPEETRLKYRFLDLRGARLHQNIVLRSKVIQELRNQMLSN
GFIEFQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLIMMSGFDKYFQIAPCFRDEDARADRSPGEFYQLDI
EMAFVTQEDVFGLVEPVLYNTFKKFSNYSITDIPFPRITYDESMLRYGSDKPDLRNPIQISDVTDIFQCSEFTTLKDSIN
CGAVVRAIPAPMSANRPRSFFDKMIEYAKHLGASGLAYIQFTNDGVKGSIAKFFDEALLARIKKLVKCQDGDAIFFICDK
EDDATKVAAKIRAKVGEELNIIKTDCYKFCWVIDFPFYQLDSEVNKITFSHNPFSMPQGGIEAFNKAKTVEDLLSIKAFQ
YDIVCNGVELSSGAIRNHKPDLMYKAFAIAGYSKDEVDKQFGAMIRAFSYGAPPHGGIAPGIDRILMLLTNSVNIREVIA
FPMNQQAEDLLMGAPSAVTEKQLQELQLKLISKERIK

Sequences:

>Translated_597_residues
MHKYRTHTCGELNLAHVGLQVKLSGWLYRRRDHGGLLFIDLRDHYGIIQLVFNDDCSIIQAEISKIKFETVITVEGTVIA
RSNETINKNISTGDVEVVVSSYVVESFAEELPLQINGEYEAPEETRLKYRFLDLRGARLHQNIVLRSKVIQELRNQMLSN
GFIEFQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLIMMSGFDKYFQIAPCFRDEDARADRSPGEFYQLDI
EMAFVTQEDVFGLVEPVLYNTFKKFSNYSITDIPFPRITYDESMLRYGSDKPDLRNPIQISDVTDIFQCSEFTTLKDSIN
CGAVVRAIPAPMSANRPRSFFDKMIEYAKHLGASGLAYIQFTNDGVKGSIAKFFDEALLARIKKLVKCQDGDAIFFICDK
EDDATKVAAKIRAKVGEELNIIKTDCYKFCWVIDFPFYQLDSEVNKITFSHNPFSMPQGGIEAFNKAKTVEDLLSIKAFQ
YDIVCNGVELSSGAIRNHKPDLMYKAFAIAGYSKDEVDKQFGAMIRAFSYGAPPHGGIAPGIDRILMLLTNSVNIREVIA
FPMNQQAEDLLMGAPSAVTEKQLQELQLKLISKERIK
>Mature_597_residues
MHKYRTHTCGELNLAHVGLQVKLSGWLYRRRDHGGLLFIDLRDHYGIIQLVFNDDCSIIQAEISKIKFETVITVEGTVIA
RSNETINKNISTGDVEVVVSSYVVESFAEELPLQINGEYEAPEETRLKYRFLDLRGARLHQNIVLRSKVIQELRNQMLSN
GFIEFQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLIMMSGFDKYFQIAPCFRDEDARADRSPGEFYQLDI
EMAFVTQEDVFGLVEPVLYNTFKKFSNYSITDIPFPRITYDESMLRYGSDKPDLRNPIQISDVTDIFQCSEFTTLKDSIN
CGAVVRAIPAPMSANRPRSFFDKMIEYAKHLGASGLAYIQFTNDGVKGSIAKFFDEALLARIKKLVKCQDGDAIFFICDK
EDDATKVAAKIRAKVGEELNIIKTDCYKFCWVIDFPFYQLDSEVNKITFSHNPFSMPQGGIEAFNKAKTVEDLLSIKAFQ
YDIVCNGVELSSGAIRNHKPDLMYKAFAIAGYSKDEVDKQFGAMIRAFSYGAPPHGGIAPGIDRILMLLTNSVNIREVIA
FPMNQQAEDLLMGAPSAVTEKQLQELQLKLISKERIK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=601, Percent_Identity=35.108153078203, Blast_Score=360, Evalue=3e-99,
Organism=Homo sapiens, GI45439306, Length=240, Percent_Identity=30.8333333333333, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=22.9249011857708, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=595, Percent_Identity=41.8487394957983, Blast_Score=470, Evalue=1e-133,
Organism=Escherichia coli, GI1790571, Length=352, Percent_Identity=25.2840909090909, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789256, Length=320, Percent_Identity=24.375, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=601, Percent_Identity=31.9467554076539, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=30, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325153, Length=656, Percent_Identity=30.9451219512195, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321807, Length=291, Percent_Identity=25.4295532646048, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=242, Percent_Identity=28.5123966942149, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24584738, Length=600, Percent_Identity=34.8333333333333, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI17136276, Length=243, Percent_Identity=27.1604938271605, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67520; Mature: 67520

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYRTHTCGELNLAHVGLQVKLSGWLYRRRDHGGLLFIDLRDHYGIIQLVFNDDCSIIQ
CCCCCCCCCCCCEEEEEEEEEEEECEEEEEECCCCEEEEEECCCCCEEEEEECCCCHHHH
AEISKIKFETVITVEGTVIARSNETINKNISTGDVEVVVSSYVVESFAEELPLQINGEYE
HHHHHEEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCC
APEETRLKYRFLDLRGARLHQNIVLRSKVIQELRNQMLSNGFIEFQTPILTASSPEGARD
CCHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCCCHH
FLVPSRLHPGKFYALPQAPQQFKQLIMMSGFDKYFQIAPCFRDEDARADRSPGEFYQLDI
CCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEE
EMAFVTQEDVFGLVEPVLYNTFKKFSNYSITDIPFPRITYDESMLRYGSDKPDLRNPIQI
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCCCCCCCCEEE
SDVTDIFQCSEFTTLKDSINCGAVVRAIPAPMSANRPRSFFDKMIEYAKHLGASGLAYIQ
CCHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
FTNDGVKGSIAKFFDEALLARIKKLVKCQDGDAIFFICDKEDDATKVAAKIRAKVGEELN
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCC
IIKTDCYKFCWVIDFPFYQLDSEVNKITFSHNPFSMPQGGIEAFNKAKTVEDLLSIKAFQ
EEECCCEEEEEEEECCHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
YDIVCNGVELSSGAIRNHKPDLMYKAFAIAGYSKDEVDKQFGAMIRAFSYGAPPHGGIAP
EEEEECCEEECCCCCCCCCCCHHEEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
GIDRILMLLTNSVNIREVIAFPMNQQAEDLLMGAPSAVTEKQLQELQLKLISKERIK
HHHHHHHHHHCCCCHHHHEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHKYRTHTCGELNLAHVGLQVKLSGWLYRRRDHGGLLFIDLRDHYGIIQLVFNDDCSIIQ
CCCCCCCCCCCCEEEEEEEEEEEECEEEEEECCCCEEEEEECCCCCEEEEEECCCCHHHH
AEISKIKFETVITVEGTVIARSNETINKNISTGDVEVVVSSYVVESFAEELPLQINGEYE
HHHHHEEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCC
APEETRLKYRFLDLRGARLHQNIVLRSKVIQELRNQMLSNGFIEFQTPILTASSPEGARD
CCHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCCCHH
FLVPSRLHPGKFYALPQAPQQFKQLIMMSGFDKYFQIAPCFRDEDARADRSPGEFYQLDI
CCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEE
EMAFVTQEDVFGLVEPVLYNTFKKFSNYSITDIPFPRITYDESMLRYGSDKPDLRNPIQI
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCCCCCCCCEEE
SDVTDIFQCSEFTTLKDSINCGAVVRAIPAPMSANRPRSFFDKMIEYAKHLGASGLAYIQ
CCHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
FTNDGVKGSIAKFFDEALLARIKKLVKCQDGDAIFFICDKEDDATKVAAKIRAKVGEELN
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCC
IIKTDCYKFCWVIDFPFYQLDSEVNKITFSHNPFSMPQGGIEAFNKAKTVEDLLSIKAFQ
EEECCCEEEEEEEECCHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
YDIVCNGVELSSGAIRNHKPDLMYKAFAIAGYSKDEVDKQFGAMIRAFSYGAPPHGGIAP
EEEEECCEEECCCCCCCCCCCHHEEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
GIDRILMLLTNSVNIREVIAFPMNQQAEDLLMGAPSAVTEKQLQELQLKLISKERIK
HHHHHHHHHHCCCCHHHHEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA