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The map label for this gene is dnaB [H]

Identifier: 189184638

GI number: 189184638

Start: 1752043

End: 1753479

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_1731

Alternate gene names: 189184638

Gene position: 1753479-1752043 (Counterclockwise)

Preceding gene: 189184639

Following gene: 189184637

Centisome position: 87.28

GC content: 31.94

Gene sequence:

>1437_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCGGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCGGAACATTTTTATGAACCATTACATGGCAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAAGCAATGAACCCGCATTTGAT
GAAATAGGTGGAGTAGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGAGGCGTTATTTAATTGAAATTGGAGAAAAAATAGTAACAAATGCGTATTCTTCTACTTTAG
CAGATTTAGCTATAAGTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGTAAAGGA
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGAC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACCTAGCAATAAATGCTTGTAAATATTTTCTTACTCAAAAAAATACTAAA
GATAATGTAGTGCCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCTACTCGAATTCTTTCTATAGA
ATCAGAAATTAATAGCTCTGCATTATTTAACGGTAAAATAGGTGAACAAGATATTGATAAGTTAAAGACTGTACAAGACG
AAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCGGCAATCTCAATATCTGCAATTAGATCTCTAGCTCGTAGACTT
AAACGTACTCATAATTTAGCAATATTATTTGTTGATTATTTACAGCTAATAAAAATTGATAACAGAAGAAGTCAGTATAA
TCGAGTGCAGGAAATTTCTGAAATTAGTCAGAGCTTAAAAGCTCTTGCTAAAGAGCTCAATATTCCAGTCATTGCATTAT
CTCAATTGTCTAGAGCTGTAGAACAAAGGTCAGATAAAAAGCCTATTCTCTCAGATCTAAGAGAATCAGGCTCAATTGAA
CAGGACGCCGATATTGTAATGCTTATATATAGAGAAGAGTATTATTTATCTAGATCAGAACCGCATCCAGATTCTATGGA
ATACGAAGAATGGTTCATCAAACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACCGTAATGGGCCAG
TTGGTACAGTAAACTTACACTATGATAATAGGTATTCTAAATTTGGCAATATTGTTAAAAACTCTCAGCAAGGTTAA

Upstream 100 bases:

>100_bases
CAAGCAACCATATGGAAAATCTTGCGCACGCTTTTAAAGCTCAGGGATTTTCTTTTGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TAAACACTAGATTATGCATAAATGAAAAAATGCTTGTCCAAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAGAG
ATATGCAACTTAAGGTTAAT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLSNEPAFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDIDKLKTVQDEIQKWNFFIDDAPAISISAIRSLARRL
KRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEISQSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIE
QDADIVMLIYREEYYLSRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG

Sequences:

>Translated_478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLSNEPAFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDIDKLKTVQDEIQKWNFFIDDAPAISISAIRSLARRL
KRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEISQSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIE
QDADIVMLIYREEYYLSRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG
>Mature_478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLSNEPAFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDIDKLKTVQDEIQKWNFFIDDAPAISISAIRSLARRL
KRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEISQSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIE
QDADIVMLIYREEYYLSRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=35.8811040339703, Blast_Score=286, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53507; Mature: 53507

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLSNEPAFDEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDIDKLKTVQDEIQKWNF
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCE
FIDDAPAISISAIRSLARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEISQSLK
EECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIEQDADIVMLIYREEYYLSRSE
HHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCC
PHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG
CCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLSNEPAFDEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLGSRGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDIDKLKTVQDEIQKWNF
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCE
FIDDAPAISISAIRSLARRLKRTHNLAILFVDYLQLIKIDNRRSQYNRVQEISEISQSLK
EECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIEQDADIVMLIYREEYYLSRSE
HHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCC
PHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG
CCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA