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The map label for this gene is spoT [H]

Identifier: 189184604

GI number: 189184604

Start: 1722559

End: 1722954

Strand: Reverse

Name: spoT [H]

Synonym: OTT_1697

Alternate gene names: 189184604

Gene position: 1722954-1722559 (Counterclockwise)

Preceding gene: 189184605

Following gene: 189184603

Centisome position: 85.76

GC content: 31.06

Gene sequence:

>396_bases
ATGGTATCAGACTACAGCTTTGAAACAGATACAATTATTACTGCAATACTACATGATACACTTGAAGACACAAAACTAAC
TAAAGAAAGAATAAGGTACGAATTTGGTAAAAAAATTGCAGAACAGGTTTCAGACCTTACCAGAGTTAGGGATGATAAGA
AAATCAGTGCTATGGAAATGATACAAATATTACGCAGCCAAAATAAAACAGAACTATTACTGATCAAGCTTTTTGATAGA
TTCCATAATATTACAACTATATTCATCAAACCTCCTTACAAAAGGCAAGAAATAATATTTGAAACTCAGCAAGAATTTAT
AGCTCTTGCTAAATATCTTAAACTACCAGAGATTGGAGAACGCTTAAGTGAATATTGTAAACTTCATGCTAGTTAA

Upstream 100 bases:

>100_bases
TTGATAAAGTTGAAAGAGCAATAAAATATGCTAAAAACATGGTCAGCAAAAGAGAGATACTGGAGAACTCTACTACACAC
ATCCATTAGAAGTAGCGTAC

Downstream 100 bases:

>100_bases
AATGTAGTAAATAATTGAGAATGAAAGGATTAAATATATGATATTAAAATCTAAGACTAAGTATACCTTACATAGCGCTA
TTCAAGATGGTGATATTAAA

Product: RelA/SpoT-related protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGKKIAEQVSDLTRVRDDKKISAMEMIQILRSQNKTELLLIKLFDR
FHNITTIFIKPPYKRQEIIFETQQEFIALAKYLKLPEIGERLSEYCKLHAS

Sequences:

>Translated_131_residues
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGKKIAEQVSDLTRVRDDKKISAMEMIQILRSQNKTELLLIKLFDR
FHNITTIFIKPPYKRQEIIFETQQEFIALAKYLKLPEIGERLSEYCKLHAS
>Mature_131_residues
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGKKIAEQVSDLTRVRDDKKISAMEMIQILRSQNKTELLLIKLFDR
FHNITTIFIKPPYKRQEIIFETQQEFIALAKYLKLPEIGERLSEYCKLHAS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=136, Percent_Identity=32.3529411764706, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 15530; Mature: 15530

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGKKIAEQVSDLTRVRDDKKISAMEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IQILRSQNKTELLLIKLFDRFHNITTIFIKPPYKRQEIIFETQQEFIALAKYLKLPEIGE
HHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
RLSEYCKLHAS
HHHHHHHHCCC
>Mature Secondary Structure
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGKKIAEQVSDLTRVRDDKKISAMEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IQILRSQNKTELLLIKLFDRFHNITTIFIKPPYKRQEIIFETQQEFIALAKYLKLPEIGE
HHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
RLSEYCKLHAS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]