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The map label for this gene is spoT [H]

Identifier: 189184556

GI number: 189184556

Start: 1681748

End: 1682362

Strand: Reverse

Name: spoT [H]

Synonym: OTT_1649

Alternate gene names: 189184556

Gene position: 1682362-1681748 (Counterclockwise)

Preceding gene: 189184557

Following gene: 189184555

Centisome position: 83.74

GC content: 29.59

Gene sequence:

>615_bases
ATGATAACATATTCAACATGTTACTACAAAACGCCAGTTTCAGATTTTAGTCAAATTCTATTAAATAAACTGATACTAAT
AAATCAACATACACAAGGATATATTAATCTTTATGAAGTTACAAAGGCAATAAAATATGCTAAAAAATATCATGGCCAGC
AAAAGAGAGATACAGGAGAGCCATATTATATACATCCATTGGAAGTAGCCTGCACAGTAGCAGAATACAGCTTTGAAACT
GATACGATTATTACAGCCATACTACATGATACAATCGAAGATACAACATTAACTAAAGAAAAAATTGCTAAGGTCTTTGG
TGTAAAGATTGCAGAACAGGTTTTTGACCTTACCAGAATTAGAAATAATAAAAAAATCAGTTCTAGAGAAATGATTAAAA
TATTGCGTCAGCAAAACAAAAAAGATTTGTTATTAATCAAATTATGCGACCGTTTACATAACATTCAAACCGTATTCATA
AAATCTGATGAAAAAAGACAAAAAATCATCATTGAAACTGAGCAAGAATTTATACCTCTTGCTCGACACCTTAAACTATC
AAAGATTGCTGATAAGTTAAGTGGATACTGTTTACTTAATACTTATCTGGAATAA

Upstream 100 bases:

>100_bases
TAAACAGTATCCACTTAACTTATCAGCAATCTTTGATAGTTTAAGGTATCGTGCCTATTTAACAATCTACCAATATGATC
CAATTAAAGATGGAGGCAAA

Downstream 100 bases:

>100_bases
ATGCTAATTCAAAAGAACAACAATATTTTATTTTTAAATAAATATTGCTGTTTCAAAAAAAGTAGCATTGTTTTTTGAAC
TCTAAGATTTAAGACTATAT

Product: RelA/SpoT-related protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MITYSTCYYKTPVSDFSQILLNKLILINQHTQGYINLYEVTKAIKYAKKYHGQQKRDTGEPYYIHPLEVACTVAEYSFET
DTIITAILHDTIEDTTLTKEKIAKVFGVKIAEQVFDLTRIRNNKKISSREMIKILRQQNKKDLLLIKLCDRLHNIQTVFI
KSDEKRQKIIIETEQEFIPLARHLKLSKIADKLSGYCLLNTYLE

Sequences:

>Translated_204_residues
MITYSTCYYKTPVSDFSQILLNKLILINQHTQGYINLYEVTKAIKYAKKYHGQQKRDTGEPYYIHPLEVACTVAEYSFET
DTIITAILHDTIEDTTLTKEKIAKVFGVKIAEQVFDLTRIRNNKKISSREMIKILRQQNKKDLLLIKLCDRLHNIQTVFI
KSDEKRQKIIIETEQEFIPLARHLKLSKIADKLSGYCLLNTYLE
>Mature_204_residues
MITYSTCYYKTPVSDFSQILLNKLILINQHTQGYINLYEVTKAIKYAKKYHGQQKRDTGEPYYIHPLEVACTVAEYSFET
DTIITAILHDTIEDTTLTKEKIAKVFGVKIAEQVFDLTRIRNNKKISSREMIKILRQQNKKDLLLIKLCDRLHNIQTVFI
KSDEKRQKIIIETEQEFIPLARHLKLSKIADKLSGYCLLNTYLE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=178, Percent_Identity=34.8314606741573, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 23864; Mature: 23864

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITYSTCYYKTPVSDFSQILLNKLILINQHTQGYINLYEVTKAIKYAKKYHGQQKRDTGE
CCEEEEEEEECCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PYYIHPLEVACTVAEYSFETDTIITAILHDTIEDTTLTKEKIAKVFGVKIAEQVFDLTRI
CEEEEHHHHEEEHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RNNKKISSREMIKILRQQNKKDLLLIKLCDRLHNIQTVFIKSDEKRQKIIIETEQEFIPL
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECHHHHHHH
ARHLKLSKIADKLSGYCLLNTYLE
HHHHHHHHHHHHHCCEEEEEECCC
>Mature Secondary Structure
MITYSTCYYKTPVSDFSQILLNKLILINQHTQGYINLYEVTKAIKYAKKYHGQQKRDTGE
CCEEEEEEEECCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PYYIHPLEVACTVAEYSFETDTIITAILHDTIEDTTLTKEKIAKVFGVKIAEQVFDLTRI
CEEEEHHHHEEEHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RNNKKISSREMIKILRQQNKKDLLLIKLCDRLHNIQTVFIKSDEKRQKIIIETEQEFIPL
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECHHHHHHH
ARHLKLSKIADKLSGYCLLNTYLE
HHHHHHHHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]