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The map label for this gene is traA [H]

Identifier: 189184551

GI number: 189184551

Start: 1677641

End: 1678564

Strand: Reverse

Name: traA [H]

Synonym: OTT_1644

Alternate gene names: 189184551

Gene position: 1678564-1677641 (Counterclockwise)

Preceding gene: 189184552

Following gene: 189184550

Centisome position: 83.55

GC content: 34.09

Gene sequence:

>924_bases
TTGCTTAGCACTAGTGGAGTTAGAGTCTTAAGAGGAAGAGCTGGTACAGGAAAATCTTATGTTTTAATAAAAGCGCATAA
GCTTGCAACAAATCGTGGACAAAAAGTTATTGGTCTTGCTCCTACTCATAAGGCAGTATCAGAGCTGAGGAGCAAAGGTT
ATACAGAGGTCTATACAGTAAAAGGATTTTTATATAATCGAAAAAAAATTTTTATGCAAGACAGCTTAATAGTAGTAGAT
GAAGCTGGAATGGTAGGTACTAAAGCTTATGCAGAGCTGTTTAGAGTAGTTAGAAACAATAATTGTCAACTGATACTTGC
TGGAGATGAAAAACAGCTAGCTTCAATAGAAAGAGGTGGAATGTTTGAGATGCTGAGTAATATTTTTGGTTCACATGTTT
TAGTAAATATTCGAAGACAAAGTAAAAACTGGAGCAGAGAAGCAGCAATGGAGTTTGCTGAGAGTAATATTTTAAGCGGT
ATAACCTTACTGAGGCAAAATAACTGCGTTAGGTTTGATAATACGTTGCAGGACTCAATGAGTAAATTAATATACAACTG
GAGTCTAAGCAAATTTAAACCACATGAAAAATTGGTAATTACAGTACGTAATAAAGATGTCGACATTCTTAATTCAAGTA
TTAGGTCTTTGTTAAAAGCAAATGGTACGCTAAAAGGCACAGAATATGAGCGTTCTATTGATGGAAGGAAAAAGTCATAT
ATGGCAGGAGATAGAATCGTATTTCAAAAAAGCTATAAGGATTTACAAATACAAAATAGTGAATTTGCAACTTTAACTTC
GGTTAGTAAAAATGAATTTATAGCTAAGACAGATGCAGGAAAAGAGGTGAGTTTTGATTCGGTAAAATACAATTTAAACA
TGGCTATGCAAGTACTGTTTATAAGGTCCAGGGAGCTTCTGTAA

Upstream 100 bases:

>100_bases
GAATAATCAGGTTTATTACAACGATATTTACAATCTTAAAAGTGATATCGAAGGTCTAGCAAATGTTAGTGAGGAACAGA
AACAAGCGCTAAGGCATATT

Downstream 100 bases:

>100_bases
AAGATGTATACGTTTTGCATAACGGAGTAAGTAATATAAGCAGCTCATACGTAGCTATGACAAGGCATATAGAGAACTTG
CAGCTATATTGCAATAAGAA

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQDSLIVVD
EAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSKNWSREAAMEFAESNILSG
ITLLRQNNCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSY
MAGDRIVFQKSYKDLQIQNSEFATLTSVSKNEFIAKTDAGKEVSFDSVKYNLNMAMQVLFIRSRELL

Sequences:

>Translated_307_residues
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQDSLIVVD
EAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSKNWSREAAMEFAESNILSG
ITLLRQNNCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSY
MAGDRIVFQKSYKDLQIQNSEFATLTSVSKNEFIAKTDAGKEVSFDSVKYNLNMAMQVLFIRSRELL
>Mature_307_residues
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQDSLIVVD
EAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSKNWSREAAMEFAESNILSG
ITLLRQNNCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSY
MAGDRIVFQKSYKDLQIQNSEFATLTSVSKNEFIAKTDAGKEVSFDSVKYNLNMAMQVLFIRSRELL

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 34677; Mature: 34677

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTV
CCCCCCCEEEECCCCCCCEEEEEEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEEE
KGFLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGG
EEEEECCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCC
MFEMLSNIFGSHVLVNIRRQSKNWSREAAMEFAESNILSGITLLRQNNCVRFDNTLQDSM
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHH
SKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSY
HHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHH
MAGDRIVFQKSYKDLQIQNSEFATLTSVSKNEFIAKTDAGKEVSFDSVKYNLNMAMQVLF
CCCCEEEEEECCCCEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEHHHHEEEEE
IRSRELL
EHHHCCC
>Mature Secondary Structure
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTV
CCCCCCCEEEECCCCCCCEEEEEEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEEE
KGFLYNRKKIFMQDSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGG
EEEEECCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCC
MFEMLSNIFGSHVLVNIRRQSKNWSREAAMEFAESNILSGITLLRQNNCVRFDNTLQDSM
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHH
SKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSY
HHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHH
MAGDRIVFQKSYKDLQIQNSEFATLTSVSKNEFIAKTDAGKEVSFDSVKYNLNMAMQVLF
CCCCEEEEEECCCCEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEHHHHEEEEE
IRSRELL
EHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8763953; 11743193; 11743194 [H]