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The map label for this gene is traD [H]

Identifier: 189184391

GI number: 189184391

Start: 1513498

End: 1515252

Strand: Reverse

Name: traD [H]

Synonym: OTT_1484

Alternate gene names: 189184391

Gene position: 1515252-1513498 (Counterclockwise)

Preceding gene: 189184392

Following gene: 189184388

Centisome position: 75.42

GC content: 32.76

Gene sequence:

>1755_bases
ATGAATTTTCAGAATCAAGGAAATTTCACCAGAGGATCGCAGTTATTTGCCCATAAGTTAAGAATGTTTGGGCAAGGTAG
TACCAACGTTTTTATAATTGGATTAGGATTATCGATATTCTGGATTATATGTCGATTATATCAAAAAGTTTTTCTGAGCA
GCTTGTATTATTTTGTAATTGAAAGATATGTGCAGCTTAAGCTAGCAATTGGTGAGCACTTTTATGATATCGATCAAATA
GGCATTAAGTTTTATAGTTTAAGGTTTAAAAAATGGATGCACCTCAATGCTCAAGACTTTTTGCATGAATTTTATACAAG
CCAACATGGATTTAAAATACAGCAATTATTGGAATTCTTAATTAATTCAGCATTGTTAGAAGGATTAATTGTTTTTGCTA
TTGGTGTGATAATCTCAATTGTTTTCTTTACAGCTCAAGGCAAAAACACGATTATTAAGGCCAAAATTAGAGGTGCTGAT
TTTGTAGAGTGCAAATGCCTATCTAAAATGCTAAAAAGCGCTAAAAAGGCCTCGAAAATCTGTTTTGGAGGCTTGCCATT
AGTAAAGAATAGTGAAAGATTACATATTCTGATTACTGGAACAACAGGTACTGGTAAAACTAATATGCTTAACGAACTGC
TACCACAAATTCGATTACATAAAGATCGAGCAATAATTGTAGATACAACTGGAGCTTTTACTGATAGATTTTTTGATTCT
AAATGTGATAAGCTGCTTAATCCTTTGGAAAAAAATAGTGAGCAATGGTTGCCTTGGAATGATTGTTTTGAAGCAGCTAA
TTTTCATGATATAGCTAGTAGTTTTAGTAATTATACTCCTAAACTTGATGACTTTTTTGCTAAAAATGCTGAATTAGTCT
TATCTGAAGCATTGAAGCTATATCAGGATGATAAAGATATCATAAAACTAATTCATACAATCATTTACTCTGATAATAGA
CAATTTGCAAAAGCTTTTAGAAACACAGCTGTATCAGGTATTATAAGCGAAAGTGCGCTTGAAACTTCTGCAGGAATTCA
GTCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGAGGTAGTTTTAGCATCAAGGAATGGTTTAGTA
ATTCGAATGAAACTGGCTGGCTATTTATCACAGCTACCCCAAGTCAAAGAGCTACTTTATGCCCACTTATTTCAGCCTGG
ATAAGCATAGCTATCAAGGCTTTGATGTGTAGAAATCCTAATCATGATAACAAAAACATGTGGTTTATACTTGATGAATT
GCCAGCTCTACAAAAAGTTTCATCTTTACCAGTTGCTTTAGCTGAAAGTAGAAAGTATGGAGGCTGCTTTGTTGCTGTAT
TGCAGAACATTCATCAATTAGAAGCAATATATGGGGCTGCTGAATGTGCTTCTATGCTGGATTTGTTTAATAGTAAATTT
ATTTTTCGAGTTAGTGATCAGGTTACAGCTTATAAGTCAGCATTAACACTAGGTGAGCAAGAAATTATTGAAACTCAAGA
GAACTTGTCATATGGATCAAATACTATGCGAGATGGGGTAAATATGAATAATGTTGAGCGTAAAAAGATTTTAGTTATGC
CATCTGAAATTATGAACCTACCAGACTTAACTTGTTATGTAAAACTTGCTGGTAACTTTCCTATCACAAAACTAACTATG
CAGCTACAAAACTTAAATACAGCTTTTGTTTGTGAATATAAATTGCTCAAAAAACTTAAGTTAGTAGAGTATTAA

Upstream 100 bases:

>100_bases
ATGAAGCAACAATTAAAAGGTTAACAGAATTAGAGAATTTTACTATTGCAAAAGAATTTGAGCAAAAACTACAGATATTG
AAAAAATACTCTTGAGATAG

Downstream 100 bases:

>100_bases
TTCGAAAAATTAATACTCTATGTTATTTTTTAAATTAACCACTTCTTTTTTTGATAACCCAGTATTTTCAGAAATAAATT
CAACTGAAAAGCCAGCTTTT

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFVIERYVQLKLAIGEHFYDIDQI
GIKFYSLRFKKWMHLNAQDFLHEFYTSQHGFKIQQLLEFLINSALLEGLIVFAIGVIISIVFFTAQGKNTIIKAKIRGAD
FVECKCLSKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAANFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNR
QFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITATPSQRATLCPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAVLQNIHQLEAIYGAAECASMLDLFNSKF
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTM
QLQNLNTAFVCEYKLLKKLKLVEY

Sequences:

>Translated_584_residues
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFVIERYVQLKLAIGEHFYDIDQI
GIKFYSLRFKKWMHLNAQDFLHEFYTSQHGFKIQQLLEFLINSALLEGLIVFAIGVIISIVFFTAQGKNTIIKAKIRGAD
FVECKCLSKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAANFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNR
QFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITATPSQRATLCPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAVLQNIHQLEAIYGAAECASMLDLFNSKF
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTM
QLQNLNTAFVCEYKLLKKLKLVEY
>Mature_584_residues
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFVIERYVQLKLAIGEHFYDIDQI
GIKFYSLRFKKWMHLNAQDFLHEFYTSQHGFKIQQLLEFLINSALLEGLIVFAIGVIISIVFFTAQGKNTIIKAKIRGAD
FVECKCLSKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAANFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNR
QFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITATPSQRATLCPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAVLQNIHQLEAIYGAAECASMLDLFNSKF
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTM
QLQNLNTAFVCEYKLLKKLKLVEY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 66188; Mature: 66188

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFVI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
ERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTSQHGFKIQQLLEFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
INSALLEGLIVFAIGVIISIVFFTAQGKNTIIKAKIRGADFVECKCLSKMLKSAKKASKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
CFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
EECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHH
KCDKLLNPLEKNSEQWLPWNDCFEAANFHDIASSFSNYTPKLDDFFAKNAELVLSEALKL
HHHHHHCHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHH
YQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYL
HCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEE
KPGGSFSIKEWFSNSNETGWLFITATPSQRATLCPLISAWISIAIKALMCRNPNHDNKNM
CCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
WFILDELPALQKVSSLPVALAESRKYGGCFVAVLQNIHQLEAIYGAAECASMLDLFNSKF
EEEECCCCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNL
EEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECHHHCCC
PDLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
CCCEEEEEEECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFVI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
ERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWMHLNAQDFLHEFYTSQHGFKIQQLLEFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
INSALLEGLIVFAIGVIISIVFFTAQGKNTIIKAKIRGADFVECKCLSKMLKSAKKASKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
CFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
EECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHH
KCDKLLNPLEKNSEQWLPWNDCFEAANFHDIASSFSNYTPKLDDFFAKNAELVLSEALKL
HHHHHHCHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHH
YQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYL
HCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEE
KPGGSFSIKEWFSNSNETGWLFITATPSQRATLCPLISAWISIAIKALMCRNPNHDNKNM
CCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
WFILDELPALQKVSSLPVALAESRKYGGCFVAVLQNIHQLEAIYGAAECASMLDLFNSKF
EEEECCCCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNL
EEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECHHHCCC
PDLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
CCCEEEEEEECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]